Italian Kiss

RSP 11034

Grower: Italian Grower

General Information

Sample Name
Italian Kiss #1
Accession Date
November 19, 2017
Reported Plant Sex
Female
DNA Extracted From
Stem

The strain rarity visualization shows how distant the strain is from the other cultivars in the Kannapedia database. The y-axis represents genetic distance, getting farther as you go up. The width of the visualization at any position along the y-axis shows how many strains there are in the database at that genetic distance. So, a common strain will have a more bottom-heavy shape, while uncommon and rare cultivars will have a visualization that is generally shifted towards the top.

Rarity: Uncommon
Most Distant Most Similar

Chemical Information

Cannabinoid and terpenoid information provided by the grower.

Cannabinoids

No information provided.

Terpenoids

No information provided.

Genetic Information

Plant Type
Type I

The bell curve in the heterozygosity visualization shows the distribution of heterozygosity levels for cannabis cultivars in the Kannapedia database. The green line shows where this particular strain fits within the distribution. Heterozygosity is associated with heterosis (aka hybrid vigor) but also leads to the production of more variable offspring. When plants have two genetically different parents, heterozygosity levels will be higher than if it has been inbred or backcrossed repeatedly.

Heterozygosity: 0.73%
Least Heterozygous Most Heterozygous

The ratio of reads mapped to Y-contigs to reads mapped to the whole Cannabis genome (Y-ratios) has been demonstrated to be strongly correlated with plant sex typing. This plot shows the distribution of Y-ratios for all samples in our database which were sequenced with the same method (panel or WGS) as this sample and where this sample falls in the distribution.

Y-Ratio Distribution: 0.0416
male female RSP11034

This chart represents the Illumina sequence coverage over the Bt/Bd allele. These are the three regions in the cannabis genome that impact THCA, CBDA, CBGA production. Coverage over the Active CBDAS gene is highly correlated with Type II and Type III plants as described by Etienne de Meijer. Coverage over the THCA gene is highly correlated with Type I and Type II plants but is anti-correlated with Type III plants. Type I plants require coverage over the inactive CBDA loci and no coverage over the Active CBDA gene. Lack of coverage over the Active CBDA and Active THCA allele are presumed to be Type IV plants (CBGA dominant). While deletions of entire THCAS and CBDAS genes are the most common Bt:Bd alleles observed, it is possible to have plants with these genes where functional expression of the enzyme is disrupted by deactivating point mutations (Kojoma et al. 2006).

Bt/Bd Allele Coverage

This chart represents the Illumina sequence coverage over the CBCA synthase gene.

CBCAS Coverage

Variants (THCAS, CBDAS, and CBCAS)

Gene HGVS.c HGVS.p Annotation Annotation Impact Contig Contig Pos Ref/Alt Var Freq
THCAS c.187A>C p.Ile63Leu missense variant moderate contig741 4417641

IGV: Start, Jump

T/G
NGS:
0.059
C90:
0.679

Variants (Select Genes of Interest)

PKSG-2a

UniProt

c.67T>A p.Phe23Ile missense variant moderate contig700 1945567

IGV: Start, Jump

A/T
NGS:
0.825
C90:
0.904
PKSG-2a

UniProt

c.31A>T p.Thr11Ser missense variant moderate contig700 1945603

IGV: Start, Jump

T/A
NGS:
0.763
C90:
0.876
PKSG-2b

UniProt

c.1152T>A p.Asn384Lys missense variant moderate contig700 1950486

IGV: Start, Jump

A/T
NGS:
0.715
C90:
0.895
PKSG-2b

UniProt

c.1132C>G p.Leu378Val missense variant moderate contig700 1950506

IGV: Start, Jump

G/C
NGS:
0.717
C90:
0.000
PKSG-2b

UniProt

c.1117A>G p.Ile373Val missense variant moderate contig700 1950521

IGV: Start, Jump

T/C
NGS:
0.807
C90:
0.981
PKSG-2b

UniProt

c.948T>G p.Asp316Glu missense variant moderate contig700 1950690

IGV: Start, Jump

A/C
NGS:
0.456
C90:
0.000
PKSG-2b

UniProt

c.945T>G p.Ser315Arg missense variant moderate contig700 1950693

IGV: Start, Jump

A/C
NGS:
0.454
C90:
0.000
PKSG-2b

UniProt

c.944G>A p.Ser315Asn missense variant moderate contig700 1950694

IGV: Start, Jump

C/T
NGS:
0.445
C90:
0.000
PKSG-2b

UniProt

c.934C>G p.His312Asp missense variant moderate contig700 1950704

IGV: Start, Jump

G/C
NGS:
0.410
C90:
0.000
PKSG-2b

UniProt

c.31A>T p.Thr11Ser missense variant moderate contig700 1951851

IGV: Start, Jump

T/A
NGS:
0.844
C90:
0.880
PKSG-2b

UniProt

c.-2_1dupATA start lost & conservative inframe insertion high contig700 1951880

IGV: Start, Jump

A/ATAT
NGS:
0.410
C90:
0.000
PKSG-4b

UniProt

c.496A>G p.Lys166Glu missense variant moderate contig700 2721177

IGV: Start, Jump

T/C
NGS:
0.651
C90:
0.684
PKSG-4b

UniProt

c.489delT p.Phe163fs frameshift variant high contig700 2721183

IGV: Start, Jump

CA/C
NGS:
0.792
C90:
0.761
PKSG-4b

UniProt

c.485A>G p.Lys162Arg missense variant moderate contig700 2721188

IGV: Start, Jump

T/C
NGS:
0.643
C90:
0.301
PKSG-4b

UniProt

c.431T>G p.Val144Gly missense variant moderate contig700 2721242

IGV: Start, Jump

A/C
NGS:
0.575
C90:
0.679
PKSG-4b

UniProt

c.419A>G p.Asp140Gly missense variant moderate contig700 2721254

IGV: Start, Jump

T/C
NGS:
0.430
C90:
0.440
aPT4

UniProt

c.626A>G p.Asn209Ser missense variant moderate contig121 2830904

IGV: Start, Jump

A/G
NGS:
0.035
C90:
0.000
aPT4

UniProt

c.1168T>C p.Tyr390His missense variant moderate contig121 2833503

IGV: Start, Jump

T/C
NGS:
0.546
C90:
0.000
aPT1

UniProt

c.160A>C p.Thr54Pro missense variant moderate contig121 2835867

IGV: Start, Jump

A/C
NGS:
0.086
C90:
0.048
aPT1

UniProt

c.406A>G p.Ile136Val missense variant moderate contig121 2839605

IGV: Start, Jump

A/G
NGS:
0.579
C90:
0.761
aPT1

UniProt

c.670T>A p.Ser224Thr missense variant moderate contig121 2840278

IGV: Start, Jump

T/A
NGS:
0.077
C90:
0.000
aPT1

UniProt

c.727G>T p.Glu243* stop gained high contig121 2841362

IGV: Start, Jump

G/T
NGS:
0.127
C90:
0.100
aPT1

UniProt

c.864C>G p.Asn288Lys missense variant moderate contig121 2842407

IGV: Start, Jump

C/G
NGS:
0.075
C90:
0.062
HDS-2

UniProt

c.82_93delGTAACCGGAACT p.Val28_Thr31del conservative inframe deletion moderate contig95 1989748

IGV: Start, Jump

CGTAACCGGAACT/C
NGS:
0.805
C90:
0.000

Nearest genetic relatives (All Samples)

0 0.058 0.117 0.175 0.233
closely related moderately related distantly related
  1. 0.006 Italian Kiss (RSP10990)
  2. 0.167 UP Sunrise (RSP10989)
  3. 0.169 RKM-2018-011 (RSP11102)
  4. 0.173 Top 44 (SRR14708268)
  5. 0.175 Tangerine Haze (RSP10995)
  6. 0.188 Blue Dream (RSP11017)
  7. 0.188 Blue Dream (RSP11007)
  8. 0.190 Super Blue Dream (RSP11011)
  9. 0.191 Blue Dream (RSP11010)
  10. 0.193 OR 05MAY2017 (RSP10940)
  11. 0.194 Blue Dream (RSP11009)
  12. 0.196 Cheese (RSP10460)
  13. 0.197 Blue Dream (RSP11012)
  14. 0.198 Serious Happiness (RSP10763)
  15. 0.200 Blue Dream (RSP11342)
  16. 0.201 Snoops Dream (RSP11003)
  17. 0.201 Blue Dream (RSP11006)
  18. 0.202 Blue Dream (RSP11008)
  19. 0.202 Blue Dream (RSP11004)
  20. 0.205 BLACK JACK (RSP11346)

Most genetically distant strains (All Samples)

0 0.125 0.250 0.375 0.500
closely related moderately related distantly related
  1. 0.482 Cherry Blossom (RSP11311)
  2. 0.467 Cherry Blossom (RSP11328)
  3. 0.459 Cherry Blossom (RSP11334)
  4. 0.443 Cherry Blossom (RSP11333)
  5. 0.442 Cherry Blossom (RSP11323)
  6. 0.436 Cherry Blossom (RSP11309)
  7. 0.434 Cherry Blossom (RSP11300)
  8. 0.433 Cherry Blossom (RSP11312)
  9. 0.428 Cherry Blossom (RSP11314)
  10. 0.425 Cherry Blossom (RSP11324)
  11. 0.423 Right Mark (RSP11628)
  12. 0.422 Cherry Blossom (RSP11335)
  13. 0.421 Cherry Blossom (RSP11308)
  14. 0.419 Cherry Blossom (RSP11321)
  15. 0.419 Feral (RSP11205)
  16. 0.417 Cherry Blossom (RSP11318)
  17. 0.416 Cherry Blossom (RSP11298)
  18. 0.416 RKM-2018-012 (RSP11103)
  19. 0.415 CS (RSP11208)
  20. 0.414 Fatso (RSP11741)

Nearest genetic relative in Phylos dataset

Phylos Strain SRR4448527
Overlapping SNPs:
120
Concordance:
83

Nearest genetic relative in Lynch dataset

Lynch Strain SRR3495218
Overlapping SNPs:
5
Concordance:
5

Blockchain Registration Information

Transaction ID
8d5055fd6895af7b492b27daa4410a9db2ff0549759c8c1438ab5da7e4b372b0
Stamping Certificate
Download PDF (853.3 KB)
SHASUM Hash
e3f7cdaa9cb00f91416271deaa7c12db7668c004bff6bfe93cf9a2ed42403003
QR code for RSP11034

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