Black Beauty

RSP 11035

Grower: Emerald Farms

General Information

Sample Name
Black Beauty_high THCv
Accession Date
November 19, 2017
Reported Plant Sex
Female
DNA Extracted From
Flower

The strain rarity visualization shows how distant the strain is from the other cultivars in the Kannapedia database. The y-axis represents genetic distance, getting farther as you go up. The width of the visualization at any position along the y-axis shows how many strains there are in the database at that genetic distance. So, a common strain will have a more bottom-heavy shape, while uncommon and rare cultivars will have a visualization that is generally shifted towards the top.

Rarity: Rare
Most Distant Most Similar

Chemical Information

Cannabinoid and terpenoid information provided by the grower.

Cannabinoids

THC + THCA
11.6196%
CBD + CBDA
0.0749%
THCV + THCVA
7.%
CBC + CBCA
n/a
CBG + CBGA
n/a
CBN + CBNA
0.0095%

Terpenoids

No information provided.

Genetic Information

Plant Type
Type I

The bell curve in the heterozygosity visualization shows the distribution of heterozygosity levels for cannabis cultivars in the Kannapedia database. The green line shows where this particular strain fits within the distribution. Heterozygosity is associated with heterosis (aka hybrid vigor) but also leads to the production of more variable offspring. When plants have two genetically different parents, heterozygosity levels will be higher than if it has been inbred or backcrossed repeatedly.

Heterozygosity: 0.79%
Least Heterozygous Most Heterozygous

The ratio of reads mapped to Y-contigs to reads mapped to the whole Cannabis genome (Y-ratios) has been demonstrated to be strongly correlated with plant sex typing. This plot shows the distribution of Y-ratios for all samples in our database which were sequenced with the same method (panel or WGS) as this sample and where this sample falls in the distribution.

Y-Ratio Distribution: 0.0374
male female RSP11035

This chart represents the Illumina sequence coverage over the Bt/Bd allele. These are the three regions in the cannabis genome that impact THCA, CBDA, CBGA production. Coverage over the Active CBDAS gene is highly correlated with Type II and Type III plants as described by Etienne de Meijer. Coverage over the THCA gene is highly correlated with Type I and Type II plants but is anti-correlated with Type III plants. Type I plants require coverage over the inactive CBDA loci and no coverage over the Active CBDA gene. Lack of coverage over the Active CBDA and Active THCA allele are presumed to be Type IV plants (CBGA dominant). While deletions of entire THCAS and CBDAS genes are the most common Bt:Bd alleles observed, it is possible to have plants with these genes where functional expression of the enzyme is disrupted by deactivating point mutations (Kojoma et al. 2006).

Bt/Bd Allele Coverage

This chart represents the Illumina sequence coverage over the CBCA synthase gene.

CBCAS Coverage

Variants (THCAS, CBDAS, and CBCAS)

Gene HGVS.c HGVS.p Annotation Annotation Impact Contig Contig Pos Ref/Alt Var Freq
THCAS c.998C>G p.Pro333Arg missense variant moderate contig741 4416830

IGV: Start, Jump

G/C
NGS:
0.182
C90:
0.000

Variants (Select Genes of Interest)

PKSG-2a

UniProt

c.67T>A p.Phe23Ile missense variant moderate contig700 1945567

IGV: Start, Jump

A/T
NGS:
0.825
C90:
0.904
PKSG-2a

UniProt

c.31A>T p.Thr11Ser missense variant moderate contig700 1945603

IGV: Start, Jump

T/A
NGS:
0.763
C90:
0.876
PKSG-2b

UniProt

c.1152T>A p.Asn384Lys missense variant moderate contig700 1950486

IGV: Start, Jump

A/T
NGS:
0.715
C90:
0.895
PKSG-2b

UniProt

c.1132C>G p.Leu378Val missense variant moderate contig700 1950506

IGV: Start, Jump

G/C
NGS:
0.717
C90:
0.000
PKSG-2b

UniProt

c.1117A>G p.Ile373Val missense variant moderate contig700 1950521

IGV: Start, Jump

T/C
NGS:
0.807
C90:
0.981
PKSG-2b

UniProt

c.948T>G p.Asp316Glu missense variant moderate contig700 1950690

IGV: Start, Jump

A/C
NGS:
0.456
C90:
0.000
PKSG-2b

UniProt

c.945T>G p.Ser315Arg missense variant moderate contig700 1950693

IGV: Start, Jump

A/C
NGS:
0.454
C90:
0.000
PKSG-2b

UniProt

c.944G>A p.Ser315Asn missense variant moderate contig700 1950694

IGV: Start, Jump

C/T
NGS:
0.445
C90:
0.000
PKSG-2b

UniProt

c.934C>G p.His312Asp missense variant moderate contig700 1950704

IGV: Start, Jump

G/C
NGS:
0.410
C90:
0.000
PKSG-2b

UniProt

c.31A>T p.Thr11Ser missense variant moderate contig700 1951851

IGV: Start, Jump

T/A
NGS:
0.844
C90:
0.880
PKSG-2b

UniProt

c.-2_1dupATA start lost & conservative inframe insertion high contig700 1951880

IGV: Start, Jump

A/ATAT
NGS:
0.410
C90:
0.000
PKSG-4b

UniProt

c.496A>G p.Lys166Glu missense variant moderate contig700 2721177

IGV: Start, Jump

T/C
NGS:
0.651
C90:
0.684
PKSG-4b

UniProt

c.489delT p.Phe163fs frameshift variant high contig700 2721183

IGV: Start, Jump

CA/C
NGS:
0.792
C90:
0.761
PKSG-4b

UniProt

c.485A>G p.Lys162Arg missense variant moderate contig700 2721188

IGV: Start, Jump

T/C
NGS:
0.643
C90:
0.301
PKSG-4b

UniProt

c.431T>G p.Val144Gly missense variant moderate contig700 2721242

IGV: Start, Jump

A/C
NGS:
0.575
C90:
0.679
aPT4

UniProt

c.302A>G p.Asn101Ser missense variant moderate contig121 2829099

IGV: Start, Jump

A/G
NGS:
0.349
C90:
0.000
aPT1

UniProt

c.160A>C p.Thr54Pro missense variant moderate contig121 2835867

IGV: Start, Jump

A/C
NGS:
0.086
C90:
0.048
aPT1

UniProt

c.629C>T p.Thr210Ile missense variant moderate contig121 2840237

IGV: Start, Jump

C/T
NGS:
0.561
C90:
0.598
aPT1

UniProt

c.727G>T p.Glu243* stop gained high contig121 2841362

IGV: Start, Jump

G/T
NGS:
0.127
C90:
0.100
aPT1

UniProt

c.864C>G p.Asn288Lys missense variant moderate contig121 2842407

IGV: Start, Jump

C/G
NGS:
0.075
C90:
0.062
HDS-2

UniProt

c.82_93delGTAACCGGAACT p.Val28_Thr31del conservative inframe deletion moderate contig95 1989748

IGV: Start, Jump

CGTAACCGGAACT/C
NGS:
0.805
C90:
0.000

Nearest genetic relatives (All Samples)

0 0.075 0.150 0.225 0.300
closely related moderately related distantly related
  1. 0.001 Black Beauty (RSP11036)
  2. 0.002 Black Beauty (RSP11175)
  3. 0.259 Cbot-2019-006 (RSP11134)
  4. 0.265 RKM-2018-018 (RSP11110)
  5. 0.266 UnObtanium (RSP11611)
  6. 0.269 PP9 (SRR14708260)
  7. 0.269 Harlox (RSP10641)
  8. 0.275 Blueberry Cheesecake (RSP10646)
  9. 0.275 RKM-2018-007 (RSP11098)
  10. 0.276 Lemon G13 (RSP11465)
  11. 0.276 Hindu Kush (SRR14708261)
  12. 0.278 Northern Lights #5 X Haze (RSP10628)
  13. 0.278 Black Jack (RSP10603)
  14. 0.278 BLACK JACK (RSP11346)
  15. 0.279 Alaska USA (RSP11171)
  16. 0.280 Grand Daddy Purple (RSP10635)
  17. 0.280 JL 4th Gen 7 (RSP11153)
  18. 0.280 RKM-2018-010 (RSP11101)
  19. 0.281 Domnesia (RSP11184)
  20. 0.281 Erez 05MAY2017 (RSP10942)

Most genetically distant strains (All Samples)

0 0.117 0.233 0.350 0.467
closely related moderately related distantly related
  1. 0.462 Cherry Blossom (RSP11328)
  2. 0.458 Cherry Blossom (RSP11311)
  3. 0.449 Cherry Blossom (RSP11317)
  4. 0.443 Cherry Blossom (RSP11309)
  5. 0.442 Cherry Blossom (RSP11324)
  6. 0.442 Cherry Blossom (RSP11323)
  7. 0.441 Cherry Blossom (RSP11298)
  8. 0.434 Cherry Blossom (RSP11334)
  9. 0.427 Right Mark (RSP11628)
  10. 0.426 Fatso (RSP11741)
  11. 0.423 Chem 91 (RSP11185)
  12. 0.422 RKM-2018-012 (RSP11103)
  13. 0.421 Cherry Blossom (RSP11330)
  14. 0.421 Cherry Blossom (RSP11333)
  15. 0.420 GMO x Garlic Breath (RSP12507)
  16. 0.418 Cherry Blossom (RSP11301)
  17. 0.416 Absolute OG (RSP11455)
  18. 0.416 Cherry Blossom (RSP11308)
  19. 0.415 CHEM4 (RSP12090)
  20. 0.414 Red Eye OG (RSP11190)

Nearest genetic relative in Phylos dataset

Phylos Strain SRR8349430
Overlapping SNPs:
125
Concordance:
122

Nearest genetic relative in Lynch dataset

Lynch Strain SRR3495251
Overlapping SNPs:
8
Concordance:
7

Blockchain Registration Information

Transaction ID
65516112b459d18c37842e9e213b55951b8138ddee0b13e4e3b1af1c8555178a
Stamping Certificate
Download PDF (854.7 KB)
SHASUM Hash
2c1a9cb47b0f77a170a851ae8a5f59bb5041e8cc18955cf6bf40752a2055affb
QR code for RSP11035

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