Hermaphrodite_ResearchSample2

RSP 11043

Grower: Iron Laboratories

General Information

Accession Date
December 18, 2017
Reported Plant Sex
not reported
DNA Extracted From
Stem

The strain rarity visualization shows how distant the strain is from the other cultivars in the Kannapedia database. The y-axis represents genetic distance, getting farther as you go up. The width of the visualization at any position along the y-axis shows how many strains there are in the database at that genetic distance. So, a common strain will have a more bottom-heavy shape, while uncommon and rare cultivars will have a visualization that is generally shifted towards the top.

Rarity: Rare
Most Distant Most Similar

Chemical Information

Cannabinoid and terpenoid information provided by the grower.

Cannabinoids

No information provided.

Terpenoids

No information provided.

Genetic Information

Plant Type
Type I

The bell curve in the heterozygosity visualization shows the distribution of heterozygosity levels for cannabis cultivars in the Kannapedia database. The green line shows where this particular strain fits within the distribution. Heterozygosity is associated with heterosis (aka hybrid vigor) but also leads to the production of more variable offspring. When plants have two genetically different parents, heterozygosity levels will be higher than if it has been inbred or backcrossed repeatedly.

Heterozygosity: 0.86%
Least Heterozygous Most Heterozygous

The ratio of reads mapped to Y-contigs to reads mapped to the whole Cannabis genome (Y-ratios) has been demonstrated to be strongly correlated with plant sex typing. This plot shows the distribution of Y-ratios for all samples in our database which were sequenced with the same method (panel or WGS) as this sample and where this sample falls in the distribution.

Y-Ratio Distribution: 0.0525
male female RSP11043

This chart represents the Illumina sequence coverage over the Bt/Bd allele. These are the three regions in the cannabis genome that impact THCA, CBDA, CBGA production. Coverage over the Active CBDAS gene is highly correlated with Type II and Type III plants as described by Etienne de Meijer. Coverage over the THCA gene is highly correlated with Type I and Type II plants but is anti-correlated with Type III plants. Type I plants require coverage over the inactive CBDA loci and no coverage over the Active CBDA gene. Lack of coverage over the Active CBDA and Active THCA allele are presumed to be Type IV plants (CBGA dominant). While deletions of entire THCAS and CBDAS genes are the most common Bt:Bd alleles observed, it is possible to have plants with these genes where functional expression of the enzyme is disrupted by deactivating point mutations (Kojoma et al. 2006).

Bt/Bd Allele Coverage

This chart represents the Illumina sequence coverage over the CBCA synthase gene.

CBCAS Coverage

Variants (THCAS, CBDAS, and CBCAS)

Gene HGVS.c HGVS.p Annotation Annotation Impact Contig Contig Pos Ref/Alt Var Freq
THCAS c.998C>G p.Pro333Arg missense variant moderate contig741 4416830

IGV: Start, Jump

G/C
NGS:
0.182
C90:
0.000

Variants (Select Genes of Interest)

PKSG-4a

UniProt

c.1015T>C p.Tyr339His missense variant moderate contig700 1938426

IGV: Start, Jump

T/C
NGS:
0.004
C90:
0.000
PKSG-4a

UniProt

c.1033C>T p.Pro345Ser missense variant moderate contig700 1938444

IGV: Start, Jump

C/T
NGS:
0.002
C90:
0.000
PKSG-2a

UniProt

c.67T>A p.Phe23Ile missense variant moderate contig700 1945567

IGV: Start, Jump

A/T
NGS:
0.825
C90:
0.904
PKSG-2a

UniProt

c.31A>T p.Thr11Ser missense variant moderate contig700 1945603

IGV: Start, Jump

T/A
NGS:
0.763
C90:
0.876
aPT1

UniProt

c.406A>G p.Ile136Val missense variant moderate contig121 2839605

IGV: Start, Jump

A/G
NGS:
0.579
C90:
0.761
aPT1

UniProt

c.629C>T p.Thr210Ile missense variant moderate contig121 2840237

IGV: Start, Jump

C/T
NGS:
0.561
C90:
0.598

Nearest genetic relatives (All Samples)

0 0.067 0.133 0.200 0.267
closely related moderately related distantly related
  1. 0.006 Hermaphrodite ResearchSample2 (RSP11050)
  2. 0.126 Sunday Driver (RSP11071)
  3. 0.179 Gorilla Cookies (RSP11231)
  4. 0.184 Wedding Pie (RSP11745)
  5. 0.215 Blueberry Cheesecake (RSP10680)
  6. 0.220 Center Mark (RSP11629)
  7. 0.221 Grape Stomper (RSP11180)
  8. 0.226 Pie Hoe (RSP11073)
  9. 0.226 Peanut Butter Breath (RSP11640)
  10. 0.227 GMO x Poison Momosa (RSP12500)
  11. 0.232 TropCookies (RSP12097)
  12. 0.233 MENDO BREATH (RSP11242)
  13. 0.237 SHERBERT (RSP11355)
  14. 0.238 Pure Power Plant (RSP11265)
  15. 0.238 Dominion Skunk (RSP11354)
  16. 0.239 Power Plant (RSP11223)
  17. 0.239 Garlic (RSP11341)
  18. 0.241 GMO x The inhaler (RSP12508)
  19. 0.241 RKM-2018-034 (RSP11126)
  20. 0.243 Old Family Purple (RSP12098)

Most genetically distant strains (All Samples)

0 0.117 0.233 0.350 0.467
closely related moderately related distantly related
  1. 0.457 Cherry Blossom (RSP11318)
  2. 0.451 Cherry Blossom (RSP11311)
  3. 0.435 Cherry Blossom (RSP11328)
  4. 0.419 Cherry Blossom (RSP11323)
  5. 0.418 Cherry Blossom (RSP11312)
  6. 0.411 Avidekel 05MAY2017 (RSP10938)
  7. 0.407 Cherry Blossom (RSP11317)
  8. 0.406 Cherry Blossom (RSP11298)
  9. 0.406 Unknown--Cherry Wine---001- (RSP11268)
  10. 0.404 Cherry Blossom (RSP11301)
  11. 0.404 Tanao Sri -46- (RSP11486)
  12. 0.403 Cherry Blossom (RSP11309)
  13. 0.402 Chematonic -Cannatonic x Chemdawg- (RSP11394)
  14. 0.401 Cherry Blossom (RSP11330)
  15. 0.401 Cherry Blossom (RSP11300)
  16. 0.398 80E (RSP11213)
  17. 0.396 Cherry Blossom (RSP11325)
  18. 0.395 Cherry Blossom (RSP11326)
  19. 0.394 Cherry Blossom (RSP11327)
  20. 0.393 Unknown--Cherry Wine---003- (RSP11270)

Nearest genetic relative in Phylos dataset

Phylos Strain SRR8349373
Overlapping SNPs:
113
Concordance:
71

Nearest genetic relative in Lynch dataset

Lynch Strain SRR3495250
Overlapping SNPs:
2
Concordance:
2

Blockchain Registration Information

Transaction ID
1e8e35a423e6d78261f5b65a1c02625b1607dea743dbbf8e5599512da7cf8226
Stamping Certificate
Download PDF (859.4 KB)
SHASUM Hash
23fba41e97af7d5db574fcdeda806c284b886f67eef84309da427ff213b22757
QR code for RSP11043

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