RKM-2018-004

RSP 11095

Grower: R-Kiem Seeds

General Information

Accession Date
September 6, 2018
Reported Plant Sex
Female
DNA Extracted From
Stem

The strain rarity visualization shows how distant the strain is from the other cultivars in the Kannapedia database. The y-axis represents genetic distance, getting farther as you go up. The width of the visualization at any position along the y-axis shows how many strains there are in the database at that genetic distance. So, a common strain will have a more bottom-heavy shape, while uncommon and rare cultivars will have a visualization that is generally shifted towards the top.

Rarity: Uncommon
Most Distant Most Similar

Chemical Information

Cannabinoid and terpenoid information provided by the grower.

Cannabinoids

No information provided.

Terpenoids

No information provided.

Genetic Information

Plant Type
Type I

The bell curve in the heterozygosity visualization shows the distribution of heterozygosity levels for cannabis cultivars in the Kannapedia database. The green line shows where this particular strain fits within the distribution. Heterozygosity is associated with heterosis (aka hybrid vigor) but also leads to the production of more variable offspring. When plants have two genetically different parents, heterozygosity levels will be higher than if it has been inbred or backcrossed repeatedly.

Heterozygosity: 0.87%
Least Heterozygous Most Heterozygous

The ratio of reads mapped to Y-contigs to reads mapped to the whole Cannabis genome (Y-ratios) has been demonstrated to be strongly correlated with plant sex typing. This plot shows the distribution of Y-ratios for all samples in our database which were sequenced with the same method (panel or WGS) as this sample and where this sample falls in the distribution.

Y-Ratio Distribution: 0.0408
male female RSP11095

This chart represents the Illumina sequence coverage over the Bt/Bd allele. These are the three regions in the cannabis genome that impact THCA, CBDA, CBGA production. Coverage over the Active CBDAS gene is highly correlated with Type II and Type III plants as described by Etienne de Meijer. Coverage over the THCA gene is highly correlated with Type I and Type II plants but is anti-correlated with Type III plants. Type I plants require coverage over the inactive CBDA loci and no coverage over the Active CBDA gene. Lack of coverage over the Active CBDA and Active THCA allele are presumed to be Type IV plants (CBGA dominant). While deletions of entire THCAS and CBDAS genes are the most common Bt:Bd alleles observed, it is possible to have plants with these genes where functional expression of the enzyme is disrupted by deactivating point mutations (Kojoma et al. 2006).

Bt/Bd Allele Coverage

This chart represents the Illumina sequence coverage over the CBCA synthase gene.

CBCAS Coverage

Variants (THCAS, CBDAS, and CBCAS)

No variants to report

Variants (Select Genes of Interest)

PKSG-2a

UniProt

c.67T>A p.Phe23Ile missense variant moderate contig700 1945567

IGV: Start, Jump

A/T
NGS:
0.825
C90:
0.904
PKSG-2a

UniProt

c.31A>T p.Thr11Ser missense variant moderate contig700 1945603

IGV: Start, Jump

T/A
NGS:
0.763
C90:
0.876
PKSG-2b

UniProt

c.1152T>A p.Asn384Lys missense variant moderate contig700 1950486

IGV: Start, Jump

A/T
NGS:
0.715
C90:
0.895
PKSG-2b

UniProt

c.1132C>G p.Leu378Val missense variant moderate contig700 1950506

IGV: Start, Jump

G/C
NGS:
0.717
C90:
0.000
PKSG-2b

UniProt

c.1117A>G p.Ile373Val missense variant moderate contig700 1950521

IGV: Start, Jump

T/C
NGS:
0.807
C90:
0.981
PKSG-2b

UniProt

c.948T>G p.Asp316Glu missense variant moderate contig700 1950690

IGV: Start, Jump

A/C
NGS:
0.456
C90:
0.000
PKSG-2b

UniProt

c.945T>G p.Ser315Arg missense variant moderate contig700 1950693

IGV: Start, Jump

A/C
NGS:
0.454
C90:
0.000
PKSG-2b

UniProt

c.944G>A p.Ser315Asn missense variant moderate contig700 1950694

IGV: Start, Jump

C/T
NGS:
0.445
C90:
0.000
PKSG-2b

UniProt

c.934C>G p.His312Asp missense variant moderate contig700 1950704

IGV: Start, Jump

G/C
NGS:
0.410
C90:
0.000
PKSG-2b

UniProt

c.31A>T p.Thr11Ser missense variant moderate contig700 1951851

IGV: Start, Jump

T/A
NGS:
0.844
C90:
0.880
PKSG-2b

UniProt

c.-2_1dupATA start lost & conservative inframe insertion high contig700 1951880

IGV: Start, Jump

A/ATAT
NGS:
0.410
C90:
0.000
PKSG-4b

UniProt

c.489delT p.Phe163fs frameshift variant high contig700 2721183

IGV: Start, Jump

CA/C
NGS:
0.792
C90:
0.761
aPT1

UniProt

c.406A>G p.Ile136Val missense variant moderate contig121 2839605

IGV: Start, Jump

A/G
NGS:
0.579
C90:
0.761
aPT1

UniProt

c.629C>T p.Thr210Ile missense variant moderate contig121 2840237

IGV: Start, Jump

C/T
NGS:
0.561
C90:
0.598
HDS-2

UniProt

c.82_93delGTAACCGGAACT p.Val28_Thr31del conservative inframe deletion moderate contig95 1989748

IGV: Start, Jump

CGTAACCGGAACT/C
NGS:
0.805
C90:
0.000
HDS-2

UniProt

c.127T>G p.Ser43Ala missense variant moderate contig95 1989794

IGV: Start, Jump

T/G
NGS:
0.721
C90:
0.000

Nearest genetic relatives (All Samples)

0 0.058 0.117 0.175 0.233
closely related moderately related distantly related
  1. 0.003 RKM-2018-004 (RSP11096)
  2. 0.174 East Coast Sour Diesel (RSP10243)
  3. 0.181 RKM-2018-033 (RSP11125)
  4. 0.183 Motor Breath #15 (RSP12093)
  5. 0.183 Pai 60w (RSP11743)
  6. 0.184 Sour D (RSP11343)
  7. 0.188 New York City Deisel (RSP11225)
  8. 0.191 RKM-2018-002 (RSP11093)
  9. 0.194 Dog Patch (RSP11725)
  10. 0.197 Super Sour Diesel (RSP11191)
  11. 0.201 501st OG (RSP11241)
  12. 0.219 NSPM1 (RSP11362)
  13. 0.221 Peach Cresendo (RSP12483)
  14. 0.223 Rugburn OG (RSP11353)
  15. 0.226 CHEM4 (RSP12090)
  16. 0.227 Triangle Kush x Square Wave BX (RSP12100)
  17. 0.229 RKM-2018-012 (RSP11103)
  18. 0.230 Chem 91 (RSP11185)
  19. 0.230 unknown (RSP11432)
  20. 0.231 Golden Road (RSP11345)

Most genetically distant strains (All Samples)

0 0.117 0.233 0.350 0.467
closely related moderately related distantly related
  1. 0.447 Northern Skunk (RSP11456)
  2. 0.440 80E (RSP11213)
  3. 0.427 Cherry Blossom (RSP11317)
  4. 0.424 80E (RSP11212)
  5. 0.420 Cherry Blossom (RSP11324)
  6. 0.419 Cherry Blossom (RSP11333)
  7. 0.418 Cherry Blossom (RSP11300)
  8. 0.416 Tiger Tail -30- (RSP11484)
  9. 0.415 Tanao Sri -46- (RSP11486)
  10. 0.415 CS (RSP11208)
  11. 0.414 Cherry Fog XL (RSP11458)
  12. 0.414 Unknown--Cherry Wine---001- (RSP11268)
  13. 0.413 JL 3rd Gen Father (RSP11196)
  14. 0.413 Cherry Blossom (RSP11301)
  15. 0.411 Cherry Blossom (RSP11311)
  16. 0.408 80E (RSP11211)
  17. 0.408 Big Bud (SRR14708270)
  18. 0.406 Cherry Blossom (RSP11306)
  19. 0.406 Cherry Blossom (RSP11318)
  20. 0.403 Cherry Blossom (RSP11329)

Nearest genetic relative in Phylos dataset

Phylos Strain SRR8349234
Overlapping SNPs:
117
Concordance:
78

Nearest genetic relative in Lynch dataset

Lynch Strain SRR3495204
Overlapping SNPs:
14
Concordance:
14

Blockchain Registration Information

Transaction ID
75b62074699a431d645b42589ec75ddd839a88af3d8ff5594e4e63db7db5ae50
Stamping Certificate
Download PDF (865.4 KB)
SHASUM Hash
596c6eed73daab9da130db684cf90e597e4b8f5c8147ee90af9636e9afbc1a13
QR code for RSP11095

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