Queen Jesus

RSP 10105

Grower: ROSEBUD, EMERALD HEART FARMS

General Information

Sample Name
CBDFL50
Accession Date
February 1, 2016
Reported Plant Sex
Female
DNA Extracted From
Flower

The strain rarity visualization shows how distant the strain is from the other cultivars in the Kannapedia database. The y-axis represents genetic distance, getting farther as you go up. The width of the visualization at any position along the y-axis shows how many strains there are in the database at that genetic distance. So, a common strain will have a more bottom-heavy shape, while uncommon and rare cultivars will have a visualization that is generally shifted towards the top.

Rarity: Rare
Most Distant Most Similar

Chemical Information

Cannabinoid and terpenoid information provided by the grower.

Cannabinoids

THC + THCA
0.77%
CBD + CBDA
18.31%
THCV + THCVA
n/a
CBC + CBCA
n/a
CBG + CBGA
n/a
CBN + CBNA
n/a

Terpenoids

α-Bisabolol
0.018%
Borneol
n/a
Camphene
n/a
Carene
n/a
Caryophyllene oxide
n/a
β-Caryophyllene
0.3526%
Fenchol
0.025%
Geraniol
0.0051%
α-Humulene
0.1033%
Limonene
0.392%
Linalool
0.159%
Myrcene
0.95%
α-Phellandrene
n/a
Terpinolene
0.014%
α-Terpineol
n/a
α-Terpinene
n/a
γ-Terpinene
n/a
Total Nerolidol
n/a
Total Ocimene
n/a
α-Pinene
0.042%
β-Pinene
n/a

Genetic Information

Plant Type
Type III

The bell curve in the heterozygosity visualization shows the distribution of heterozygosity levels for cannabis cultivars in the Kannapedia database. The green line shows where this particular strain fits within the distribution. Heterozygosity is associated with heterosis (aka hybrid vigor) but also leads to the production of more variable offspring. When plants have two genetically different parents, heterozygosity levels will be higher than if it has been inbred or backcrossed repeatedly.

Heterozygosity: 1.63%
Least Heterozygous Most Heterozygous

The ratio of reads mapped to Y-contigs to reads mapped to the whole Cannabis genome (Y-ratios) has been demonstrated to be strongly correlated with plant sex typing. This plot shows the distribution of Y-ratios for all samples in our database which were sequenced with the same method (panel or WGS) as this sample and where this sample falls in the distribution.

Y-Ratio Distribution: 0.0443
male female RSP10105

This chart represents the Illumina sequence coverage over the Bt/Bd allele. These are the three regions in the cannabis genome that impact THCA, CBDA, CBGA production. Coverage over the Active CBDAS gene is highly correlated with Type II and Type III plants as described by Etienne de Meijer. Coverage over the THCA gene is highly correlated with Type I and Type II plants but is anti-correlated with Type III plants. Type I plants require coverage over the inactive CBDA loci and no coverage over the Active CBDA gene. Lack of coverage over the Active CBDA and Active THCA allele are presumed to be Type IV plants (CBGA dominant). While deletions of entire THCAS and CBDAS genes are the most common Bt:Bd alleles observed, it is possible to have plants with these genes where functional expression of the enzyme is disrupted by deactivating point mutations (Kojoma et al. 2006).

Bt/Bd Allele Coverage

This chart represents the Illumina sequence coverage over the CBCA synthase gene.

CBCAS Coverage

Variants (THCAS, CBDAS, and CBCAS)

No variants to report

Variants (Select Genes of Interest)

PKSG-2b

UniProt

c.1152T>A p.Asn384Lys missense variant moderate contig700 1950486

IGV: Start, Jump

A/T
NGS:
0.715
C90:
0.895
PKSG-2b

UniProt

c.1132C>G p.Leu378Val missense variant moderate contig700 1950506

IGV: Start, Jump

G/C
NGS:
0.717
C90:
0.000
PKSG-2b

UniProt

c.1117A>G p.Ile373Val missense variant moderate contig700 1950521

IGV: Start, Jump

T/C
NGS:
0.807
C90:
0.981
PKSG-2b

UniProt

c.944G>A p.Ser315Asn missense variant moderate contig700 1950694

IGV: Start, Jump

C/T
NGS:
0.445
C90:
0.000
PKSG-2b

UniProt

c.934C>G p.His312Asp missense variant moderate contig700 1950704

IGV: Start, Jump

G/C
NGS:
0.410
C90:
0.000
PKSG-2b

UniProt

c.31A>T p.Thr11Ser missense variant moderate contig700 1951851

IGV: Start, Jump

T/A
NGS:
0.844
C90:
0.880
PKSG-2b

UniProt

c.-2_1dupATA start lost & conservative inframe insertion high contig700 1951880

IGV: Start, Jump

A/ATAT
NGS:
0.410
C90:
0.000
aPT4

UniProt

c.775delT p.Tyr259fs frameshift variant high contig121 2831380

IGV: Start, Jump

AT/A
NGS:
0.224
C90:
0.000
aPT1

UniProt

c.406A>G p.Ile136Val missense variant moderate contig121 2839605

IGV: Start, Jump

A/G
NGS:
0.579
C90:
0.761
aPT1

UniProt

c.629C>T p.Thr210Ile missense variant moderate contig121 2840237

IGV: Start, Jump

C/T
NGS:
0.561
C90:
0.598

Nearest genetic relatives (All Samples)

0 0.067 0.133 0.200 0.267
clone distance sibling distance more distant
  1. 0.211 Ringo s Angel (RSP10085)
  2. 0.218 Trump x Trump (RSP11466)
  3. 0.218 Doug s Varin (RSP11243)
  4. 0.222 Liberty Haze (RSP11000)
  5. 0.223 Electra (RSP11366)
  6. 0.224 Lift (RSP11378)
  7. 0.226 Suver Haze (RSP11364)
  8. 0.226 UnObtanium (RSP11611)
  9. 0.228 Badger (RSP11614)
  10. 0.229 Domnesia (RSP11184)
  11. 0.229 Cheese (RSP10460)
  12. 0.231 Blue Dream (RSP11017)
  13. 0.232 Liberty Haze (RSP10946)
  14. 0.233 Rest (RSP11377)
  15. 0.235 SPQ -quite resistant- (RSP11370)
  16. 0.235 Serious Happiness (RSP10763)
  17. 0.236 Lemon G13 (RSP11465)
  18. 0.239 Big Skunk (SRR14708269)
  19. 0.240 Blue Dream (RSP11007)
  20. 0.241 Lifter (RSP11365)

Most genetically distant strains (All Samples)

0 0.108 0.217 0.325 0.433
clone distance sibling distance more distant
  1. 0.423 80E (RSP11213)
  2. 0.412 80E (RSP11212)
  3. 0.412 Cherry Blossom (RSP11328)
  4. 0.404 Cherry Blossom (RSP11309)
  5. 0.404 Cherry Blossom (RSP11317)
  6. 0.399 BagSeed (RSP12627)
  7. 0.397 80E (RSP11211)
  8. 0.395 Cherry Blossom (RSP11298)
  9. 0.392 Cherry Blossom (RSP11323)
  10. 0.385 Fatso (RSP11741)
  11. 0.385 Chem 91 (RSP11185)
  12. 0.385 GMO x Garlic Breath (RSP12507)
  13. 0.383 R1in136 (SRR14708226)
  14. 0.380 Cherry Blossom (RSP11311)
  15. 0.380 Cherry Blossom (RSP11301)
  16. 0.380 Cherry Blossom (RSP11306)
  17. 0.378 Cherry Blossom (RSP11333)
  18. 0.377 Cherry Blossom (RSP11324)
  19. 0.376 GG4 (RSP11978)
  20. 0.376 Feral (RSP11205)

Nearest genetic relative in Phylos dataset

Phylos Strain SRR8346567
Overlapping SNPs:
85
Concordance:
51

Nearest genetic relative in Lynch dataset

Lynch Strain SRR3495173
Overlapping SNPs:
6
Concordance:
6

Blockchain Registration Information

Transaction ID
a1ae367a8b87a099f5dbb7828e7bd2ab2efd505f631163f2bbcc87fe7325f7d9
Stamping Certificate
Download PDF (856.1 KB)
SHASUM Hash
24bb539eb8e019b38eb784c4e8b1a45473308708b3fe902c53deab74d461b44c
QR code for RSP10105

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