Recon

RSP 10755

Grower: DNA Genetics

General Information

Accession Date
March 13, 2017
Reported Plant Sex
Female
DNA Extracted From
Flower

The strain rarity visualization shows how distant the strain is from the other cultivars in the Kannapedia database. The y-axis represents genetic distance, getting farther as you go up. The width of the visualization at any position along the y-axis shows how many strains there are in the database at that genetic distance. So, a common strain will have a more bottom-heavy shape, while uncommon and rare cultivars will have a visualization that is generally shifted towards the top.

Rarity: Rare
Most Distant Most Similar

Chemical Information

Cannabinoid and terpenoid information provided by the grower.

Cannabinoids

No information provided.

Terpenoids

No information provided.

Genetic Information

Plant Type
Type I

The bell curve in the heterozygosity visualization shows the distribution of heterozygosity levels for cannabis cultivars in the Kannapedia database. The green line shows where this particular strain fits within the distribution. Heterozygosity is associated with heterosis (aka hybrid vigor) but also leads to the production of more variable offspring. When plants have two genetically different parents, heterozygosity levels will be higher than if it has been inbred or backcrossed repeatedly.

Heterozygosity: 1.15%
Least Heterozygous Most Heterozygous

The ratio of reads mapped to Y-contigs to reads mapped to the whole Cannabis genome (Y-ratios) has been demonstrated to be strongly correlated with plant sex typing. This plot shows the distribution of Y-ratios for all samples in our database which were sequenced with the same method (panel or WGS) as this sample and where this sample falls in the distribution.

Y-Ratio Distribution: 0.0429
male female RSP10755

This chart represents the Illumina sequence coverage over the Bt/Bd allele. These are the three regions in the cannabis genome that impact THCA, CBDA, CBGA production. Coverage over the Active CBDAS gene is highly correlated with Type II and Type III plants as described by Etienne de Meijer. Coverage over the THCA gene is highly correlated with Type I and Type II plants but is anti-correlated with Type III plants. Type I plants require coverage over the inactive CBDA loci and no coverage over the Active CBDA gene. Lack of coverage over the Active CBDA and Active THCA allele are presumed to be Type IV plants (CBGA dominant). While deletions of entire THCAS and CBDAS genes are the most common Bt:Bd alleles observed, it is possible to have plants with these genes where functional expression of the enzyme is disrupted by deactivating point mutations (Kojoma et al. 2006).

Bt/Bd Allele Coverage

This chart represents the Illumina sequence coverage over the CBCA synthase gene.

CBCAS Coverage

Variants (THCAS, CBDAS, and CBCAS)

Gene HGVS.c HGVS.p Annotation Annotation Impact Contig Contig Pos Ref/Alt Var Freq
THCAS c.1229G>A p.Gly410Glu missense variant moderate contig741 4416599

IGV: Start, Jump

C/T
NGS:
0.039
C90:
0.000

Variants (Select Genes of Interest)

PKSG-2a

UniProt

c.67T>A p.Phe23Ile missense variant moderate contig700 1945567

IGV: Start, Jump

A/T
NGS:
0.825
C90:
0.904
PKSG-2a

UniProt

c.31A>T p.Thr11Ser missense variant moderate contig700 1945603

IGV: Start, Jump

T/A
NGS:
0.763
C90:
0.876
PKSG-2b

UniProt

c.1152T>A p.Asn384Lys missense variant moderate contig700 1950486

IGV: Start, Jump

A/T
NGS:
0.715
C90:
0.895
PKSG-2b

UniProt

c.1132C>G p.Leu378Val missense variant moderate contig700 1950506

IGV: Start, Jump

G/C
NGS:
0.717
C90:
0.000
PKSG-2b

UniProt

c.1117A>G p.Ile373Val missense variant moderate contig700 1950521

IGV: Start, Jump

T/C
NGS:
0.807
C90:
0.981
PKSG-2b

UniProt

c.948T>G p.Asp316Glu missense variant moderate contig700 1950690

IGV: Start, Jump

A/C
NGS:
0.456
C90:
0.000
PKSG-2b

UniProt

c.945T>G p.Ser315Arg missense variant moderate contig700 1950693

IGV: Start, Jump

A/C
NGS:
0.454
C90:
0.000
PKSG-2b

UniProt

c.944G>A p.Ser315Asn missense variant moderate contig700 1950694

IGV: Start, Jump

C/T
NGS:
0.445
C90:
0.000
PKSG-2b

UniProt

c.934C>G p.His312Asp missense variant moderate contig700 1950704

IGV: Start, Jump

G/C
NGS:
0.410
C90:
0.000
PKSG-2b

UniProt

c.31A>T p.Thr11Ser missense variant moderate contig700 1951851

IGV: Start, Jump

T/A
NGS:
0.844
C90:
0.880
PKSG-2b

UniProt

c.-2_1dupATA start lost & conservative inframe insertion high contig700 1951880

IGV: Start, Jump

A/ATAT
NGS:
0.410
C90:
0.000
PKSG-4b

UniProt

c.496A>G p.Lys166Glu missense variant moderate contig700 2721177

IGV: Start, Jump

T/C
NGS:
0.651
C90:
0.684
PKSG-4b

UniProt

c.489delT p.Phe163fs frameshift variant high contig700 2721183

IGV: Start, Jump

CA/C
NGS:
0.792
C90:
0.761
PKSG-4b

UniProt

c.485A>G p.Lys162Arg missense variant moderate contig700 2721188

IGV: Start, Jump

T/C
NGS:
0.643
C90:
0.301
PKSG-4b

UniProt

c.431T>G p.Val144Gly missense variant moderate contig700 2721242

IGV: Start, Jump

A/C
NGS:
0.575
C90:
0.679
PKSG-4b

UniProt

c.419A>G p.Asp140Gly missense variant moderate contig700 2721254

IGV: Start, Jump

T/C
NGS:
0.430
C90:
0.440
aPT1

UniProt

c.406A>G p.Ile136Val missense variant moderate contig121 2839605

IGV: Start, Jump

A/G
NGS:
0.579
C90:
0.761

Nearest genetic relatives (All Samples)

0 0.058 0.117 0.175 0.233
clone distance sibling distance more distant
  1. 0.175 Doug s Varin (RSP11243)
  2. 0.184 Serious Happiness (RSP10763)
  3. 0.186 Electra (RSP11366)
  4. 0.196 Lift (RSP11378)
  5. 0.207 Domnesia (RSP11184)
  6. 0.212 Durban Poison #1 (RSP10996)
  7. 0.214 White Label 2 (RSP11337)
  8. 0.214 Liberty Haze (RSP11000)
  9. 0.219 Rest (RSP11377)
  10. 0.220 Joy (RSP11380)
  11. 0.221 Miss X (RSP10999)
  12. 0.221 Durban Poison (RSP11014)
  13. 0.222 JL 4th Gen 7 (RSP11153)
  14. 0.225 Liberty Haze (RSP10946)
  15. 0.225 Blueberry Cheesecake (RSP10684)
  16. 0.227 Strawberry Cough (RSP11356)
  17. 0.227 CBG-#40 (RSP11444)
  18. 0.229 Gold Cracker (RSP11048)
  19. 0.232 Gold Cracker (RSP11041)
  20. 0.232 Up All Day Jay (RSP11258)

Most genetically distant strains (All Samples)

0 0.125 0.250 0.375 0.500
clone distance sibling distance more distant
  1. 0.470 Cherry Blossom (RSP11323)
  2. 0.418 Cherry Blossom (RSP11318)
  3. 0.401 Chem 91 (RSP11185)
  4. 0.397 Unknown--Cherry Wine---001- (RSP11268)
  5. 0.396 Cherry Blossom (RSP11328)
  6. 0.385 Cherry Blossom (RSP11298)
  7. 0.384 Cherry Blossom (RSP11312)
  8. 0.379 Chematonic -Cannatonic x Chemdawg- (RSP11394)
  9. 0.378 Cherry Blossom (RSP11301)
  10. 0.377 Unknown--Cherry Wine---002- (RSP11269)
  11. 0.373 Danny Noonan (RSP11070)
  12. 0.373 Unknown--Cherry Wine---003- (RSP11270)
  13. 0.370 Cherry Blossom (RSP11311)
  14. 0.368 RKM-2018-002 (RSP11093)
  15. 0.368 JL yellow (RSP11075)
  16. 0.366 Cherry Blossom (RSP11274)
  17. 0.363 JL#2 (RSP11076)
  18. 0.363 Tanao Sri -46- (RSP11486)
  19. 0.363 Cherry Blossom (RSP11309)
  20. 0.361 Feral (RSP11205)

Nearest genetic relative in Phylos dataset

Phylos Strain SRR4448289
Overlapping SNPs:
105
Concordance:
69

Nearest genetic relative in Lynch dataset

Lynch Strain SRR3495250
Overlapping SNPs:
2
Concordance:
2

Blockchain Registration Information

Transaction ID
319e22373ec7eadecd67cd200eab05fde7d75f2721b93a927bfbc719b39d7e8b
Stamping Certificate
Download PDF (847.0 KB)
SHASUM Hash
aad0f0a734b315f9118dcdda212473005a14074617a39c8ae3ec9b2058ef8c20
QR code for RSP10755

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