Serious Happiness

RSP 10763

Grower: Alternative Therapies Group

General Information

Accession Date
March 13, 2017
Reported Plant Sex
Female
DNA Extracted From
Flower

The strain rarity visualization shows how distant the strain is from the other cultivars in the Kannapedia database. The y-axis represents genetic distance, getting farther as you go up. The width of the visualization at any position along the y-axis shows how many strains there are in the database at that genetic distance. So, a common strain will have a more bottom-heavy shape, while uncommon and rare cultivars will have a visualization that is generally shifted towards the top.

Rarity: Common
Most Distant Most Similar

Chemical Information

Cannabinoid and terpenoid information provided by the grower.

Cannabinoids

THC + THCA
16.%
CBD + CBDA
1.%
THCV + THCVA
n/a
CBC + CBCA
n/a
CBG + CBGA
n/a
CBN + CBNA
n/a

Terpenoids

No information provided.

Genetic Information

Plant Type
Type I

The bell curve in the heterozygosity visualization shows the distribution of heterozygosity levels for cannabis cultivars in the Kannapedia database. The green line shows where this particular strain fits within the distribution. Heterozygosity is associated with heterosis (aka hybrid vigor) but also leads to the production of more variable offspring. When plants have two genetically different parents, heterozygosity levels will be higher than if it has been inbred or backcrossed repeatedly.

Heterozygosity: 1.05%
Least Heterozygous Most Heterozygous

The ratio of reads mapped to Y-contigs to reads mapped to the whole Cannabis genome (Y-ratios) has been demonstrated to be strongly correlated with plant sex typing. This plot shows the distribution of Y-ratios for all samples in our database which were sequenced with the same method (panel or WGS) as this sample and where this sample falls in the distribution.

Y-Ratio Distribution: 0.0411
male female RSP10763

This chart represents the Illumina sequence coverage over the Bt/Bd allele. These are the three regions in the cannabis genome that impact THCA, CBDA, CBGA production. Coverage over the Active CBDAS gene is highly correlated with Type II and Type III plants as described by Etienne de Meijer. Coverage over the THCA gene is highly correlated with Type I and Type II plants but is anti-correlated with Type III plants. Type I plants require coverage over the inactive CBDA loci and no coverage over the Active CBDA gene. Lack of coverage over the Active CBDA and Active THCA allele are presumed to be Type IV plants (CBGA dominant). While deletions of entire THCAS and CBDAS genes are the most common Bt:Bd alleles observed, it is possible to have plants with these genes where functional expression of the enzyme is disrupted by deactivating point mutations (Kojoma et al. 2006).

Bt/Bd Allele Coverage

This chart represents the Illumina sequence coverage over the CBCA synthase gene.

CBCAS Coverage

Variants (THCAS, CBDAS, and CBCAS)

No variants to report

Variants (Select Genes of Interest)

PKSG-2a

UniProt

c.67T>A p.Phe23Ile missense variant moderate contig700 1945567

IGV: Start, Jump

A/T
NGS:
0.825
C90:
0.904
PKSG-4b

UniProt

c.496A>G p.Lys166Glu missense variant moderate contig700 2721177

IGV: Start, Jump

T/C
NGS:
0.651
C90:
0.684
PKSG-4b

UniProt

c.489delT p.Phe163fs frameshift variant high contig700 2721183

IGV: Start, Jump

CA/C
NGS:
0.792
C90:
0.761
PKSG-4b

UniProt

c.485A>G p.Lys162Arg missense variant moderate contig700 2721188

IGV: Start, Jump

T/C
NGS:
0.643
C90:
0.301
aPT1

UniProt

c.160A>C p.Thr54Pro missense variant moderate contig121 2835867

IGV: Start, Jump

A/C
NGS:
0.086
C90:
0.048
aPT1

UniProt

c.406A>G p.Ile136Val missense variant moderate contig121 2839605

IGV: Start, Jump

A/G
NGS:
0.579
C90:
0.761
aPT1

UniProt

c.629C>T p.Thr210Ile missense variant moderate contig121 2840237

IGV: Start, Jump

C/T
NGS:
0.561
C90:
0.598
HDS-2

UniProt

c.82_93delGTAACCGGAACT p.Val28_Thr31del conservative inframe deletion moderate contig95 1989748

IGV: Start, Jump

CGTAACCGGAACT/C
NGS:
0.805
C90:
0.000

Nearest genetic relatives (All Samples)

0 0.033 0.067 0.100 0.133
clone distance sibling distance more distant
  1. 0.062 Doug s Varin (RSP11243)
  2. 0.063 Electra (RSP11366)
  3. 0.075 Domnesia (RSP11184)
  4. 0.078 Lift (RSP11378)
  5. 0.080 Blue Dream (RSP11004)
  6. 0.085 Blue Dream (RSP11010)
  7. 0.092 Blue Dream (RSP11017)
  8. 0.096 Joy (RSP11380)
  9. 0.096 Blue Dream (RSP11007)
  10. 0.103 Super Blue Dream (RSP11011)
  11. 0.104 Blue Dream (RSP11006)
  12. 0.111 JL 4th Gen 7 (RSP11153)
  13. 0.112 Blue Dream (RSP11009)
  14. 0.112 Blue Dream (RSP11008)
  15. 0.114 JL X NSPM1 14 (RSP11473)
  16. 0.121 Blueberry Cheesecake (RSP10684)
  17. 0.123 Rest (RSP11377)
  18. 0.125 UnObtanium (RSP11611)
  19. 0.128 Badger (RSP11614)
  20. 0.130 Suver Haze (RSP11364)

Most genetically distant strains (All Samples)

0 0.108 0.217 0.325 0.433
clone distance sibling distance more distant
  1. 0.407 80E (RSP11213)
  2. 0.384 80E (RSP11211)
  3. 0.369 IUP3 (SRR14708256)
  4. 0.366 Feral (RSP11205)
  5. 0.366 80E (RSP11212)
  6. 0.365 IUP2 (SRR14708257)
  7. 0.365 R3in134 (SRR14708220)
  8. 0.363 R1in136 (SRR14708226)
  9. 0.362 Cherry Blossom (RSP11328)
  10. 0.359 R3in134 (SRR14708235)
  11. 0.358 R1in136 (SRR14708237)
  12. 0.356 Cbot-2019-005 (RSP11133)
  13. 0.354 IUP1 (SRR14708258)
  14. 0.354 Feral (RSP10891)
  15. 0.353 CS (RSP11208)
  16. 0.353 Feral (RSP10892)
  17. 0.353 Feral (RSP10890)
  18. 0.352 JL yellow (RSP11075)
  19. 0.351 Beniko (SRR14708275)
  20. 0.350 PCL2 (SRR14708245)

Nearest genetic relative in Phylos dataset

Phylos Strain SRR4450115
Overlapping SNPs:
77
Concordance:
59

Nearest genetic relative in Lynch dataset

Lynch Strain SRR3495196
Overlapping SNPs:
3
Concordance:
3

Blockchain Registration Information

Transaction ID
3c70b33d8182a953556a7a7e40395ec2c1746480ca50facdff3aadbdc87d5efe
Stamping Certificate
Download PDF (862.1 KB)
SHASUM Hash
7560398874cda756c78f2f4ffd35bf044e8b7da592d9a76e6a129cf0b9f0e2e9
QR code for RSP10763

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