Midnight_05MAY2017

RSP 10941

Grower: DigiPath Labs

General Information

Accession Date
May 4, 2017
Reported Plant Sex
not reported

The strain rarity visualization shows how distant the strain is from the other cultivars in the Kannapedia database. The y-axis represents genetic distance, getting farther as you go up. The width of the visualization at any position along the y-axis shows how many strains there are in the database at that genetic distance. So, a common strain will have a more bottom-heavy shape, while uncommon and rare cultivars will have a visualization that is generally shifted towards the top.

Rarity: Uncommon
Most Distant Most Similar

Chemical Information

Cannabinoid and terpenoid information provided by the grower.

Cannabinoids

No information provided.

Terpenoids

No information provided.

Genetic Information

Plant Type
Type II

The bell curve in the heterozygosity visualization shows the distribution of heterozygosity levels for cannabis cultivars in the Kannapedia database. The green line shows where this particular strain fits within the distribution. Heterozygosity is associated with heterosis (aka hybrid vigor) but also leads to the production of more variable offspring. When plants have two genetically different parents, heterozygosity levels will be higher than if it has been inbred or backcrossed repeatedly.

Heterozygosity: 1.11%
Least Heterozygous Most Heterozygous

The ratio of reads mapped to Y-contigs to reads mapped to the whole Cannabis genome (Y-ratios) has been demonstrated to be strongly correlated with plant sex typing. This plot shows the distribution of Y-ratios for all samples in our database which were sequenced with the same method (panel or WGS) as this sample and where this sample falls in the distribution.

Y-Ratio Distribution: 0.0427
male female RSP10941

This chart represents the Illumina sequence coverage over the Bt/Bd allele. These are the three regions in the cannabis genome that impact THCA, CBDA, CBGA production. Coverage over the Active CBDAS gene is highly correlated with Type II and Type III plants as described by Etienne de Meijer. Coverage over the THCA gene is highly correlated with Type I and Type II plants but is anti-correlated with Type III plants. Type I plants require coverage over the inactive CBDA loci and no coverage over the Active CBDA gene. Lack of coverage over the Active CBDA and Active THCA allele are presumed to be Type IV plants (CBGA dominant). While deletions of entire THCAS and CBDAS genes are the most common Bt:Bd alleles observed, it is possible to have plants with these genes where functional expression of the enzyme is disrupted by deactivating point mutations (Kojoma et al. 2006).

Bt/Bd Allele Coverage

This chart represents the Illumina sequence coverage over the CBCA synthase gene.

CBCAS Coverage

Variants (THCAS, CBDAS, and CBCAS)

No variants to report

Variants (Select Genes of Interest)

PKSG-2a

UniProt

c.67T>A p.Phe23Ile missense variant moderate contig700 1945567

IGV: Start, Jump

A/T
NGS:
0.825
C90:
0.904
PKSG-2a

UniProt

c.31A>T p.Thr11Ser missense variant moderate contig700 1945603

IGV: Start, Jump

T/A
NGS:
0.763
C90:
0.876
PKSG-2b

UniProt

c.1152T>A p.Asn384Lys missense variant moderate contig700 1950486

IGV: Start, Jump

A/T
NGS:
0.715
C90:
0.895
PKSG-2b

UniProt

c.1132C>G p.Leu378Val missense variant moderate contig700 1950506

IGV: Start, Jump

G/C
NGS:
0.717
C90:
0.000
PKSG-2b

UniProt

c.1117A>G p.Ile373Val missense variant moderate contig700 1950521

IGV: Start, Jump

T/C
NGS:
0.807
C90:
0.981
PKSG-2b

UniProt

c.948T>G p.Asp316Glu missense variant moderate contig700 1950690

IGV: Start, Jump

A/C
NGS:
0.456
C90:
0.000
PKSG-2b

UniProt

c.945T>G p.Ser315Arg missense variant moderate contig700 1950693

IGV: Start, Jump

A/C
NGS:
0.454
C90:
0.000
PKSG-2b

UniProt

c.944G>A p.Ser315Asn missense variant moderate contig700 1950694

IGV: Start, Jump

C/T
NGS:
0.445
C90:
0.000
PKSG-2b

UniProt

c.934C>G p.His312Asp missense variant moderate contig700 1950704

IGV: Start, Jump

G/C
NGS:
0.410
C90:
0.000
PKSG-2b

UniProt

c.31A>T p.Thr11Ser missense variant moderate contig700 1951851

IGV: Start, Jump

T/A
NGS:
0.844
C90:
0.880
PKSG-2b

UniProt

c.-2_1dupATA start lost & conservative inframe insertion high contig700 1951880

IGV: Start, Jump

A/ATAT
NGS:
0.410
C90:
0.000
PKSG-4b

UniProt

c.496A>G p.Lys166Glu missense variant moderate contig700 2721177

IGV: Start, Jump

T/C
NGS:
0.651
C90:
0.684
PKSG-4b

UniProt

c.492G>C p.Glu164Asp missense variant moderate contig700 2721181

IGV: Start, Jump

C/G
NGS:
0.048
C90:
0.000
PKSG-4b

UniProt

c.489delT p.Phe163fs frameshift variant high contig700 2721183

IGV: Start, Jump

CA/C
NGS:
0.792
C90:
0.761
PKSG-4b

UniProt

c.485A>G p.Lys162Arg missense variant moderate contig700 2721188

IGV: Start, Jump

T/C
NGS:
0.643
C90:
0.301
PKSG-4b

UniProt

c.431T>G p.Val144Gly missense variant moderate contig700 2721242

IGV: Start, Jump

A/C
NGS:
0.575
C90:
0.679
PKSG-4b

UniProt

c.419A>G p.Asp140Gly missense variant moderate contig700 2721254

IGV: Start, Jump

T/C
NGS:
0.430
C90:
0.440
PKSG-4b

UniProt

c.352_355delACAG p.Thr118fs frameshift variant high contig700 2721317

IGV: Start, Jump

CCTGT/C
NGS:
0.529
C90:
0.000
DXR-1

UniProt

c.20G>T p.Cys7Phe missense variant moderate contig380 235947

IGV: Start, Jump

C/A
NGS:
0.020
C90:
0.000
DXR-2

UniProt

c.1319T>C p.Ile440Thr missense variant moderate contig380 285250

IGV: Start, Jump

A/G
NGS:
0.480
C90:
0.000
DXR-2

UniProt

c.1140A>C p.Lys380Asn missense variant moderate contig380 285582

IGV: Start, Jump

T/G
NGS:
0.018
C90:
0.000
HDS-2

UniProt

c.82_93delGTAACCGGAACT p.Val28_Thr31del conservative inframe deletion moderate contig95 1989748

IGV: Start, Jump

CGTAACCGGAACT/C
NGS:
0.805
C90:
0.000
HDS-2

UniProt

c.127T>G p.Ser43Ala missense variant moderate contig95 1989794

IGV: Start, Jump

T/G
NGS:
0.721
C90:
0.000

Nearest genetic relatives (All Samples)

0 0.058 0.117 0.175 0.233
clone distance sibling distance more distant
  1. 0.155 Cherry Wine (RSP11305)
  2. 0.159 Cherry Wine (RSP11307)
  3. 0.183 101st AIRBORN CBD (RSP11350)
  4. 0.185 FL30 (RSP11361)
  5. 0.185 RAPHAEL 4 (RSP11173)
  6. 0.187 Avidekel (RSP10938)
  7. 0.192 Unknown- Cherry Wine - 005 (RSP11272)
  8. 0.193 Cherry Wine (RSP11145)
  9. 0.194 Wife (RSP11148)
  10. 0.200 Queen Dream CBG (RSP11281)
  11. 0.202 Cherry Blossom (RSP11299)
  12. 0.202 Cherry Blossom (RSP11332)
  13. 0.209 RAPHAEL 2 (RSP11172)
  14. 0.213 Cherry Blossom (RSP11304)
  15. 0.214 Alaska (RSP10939)
  16. 0.214 Cbot-2019-003 (RSP11131)
  17. 0.215 Durban Poison 1 (RSP11013)
  18. 0.219 Cherry Blossom (RSP11310)
  19. 0.223 AVIDEKEL USA (RSP11169)
  20. 0.224 Otto (RSP11147)

Nearest genetic relatives (Base Tree)

0 0.092 0.183 0.275 0.367
clone distance sibling distance more distant
  1. 0.243 Cbot-2019-001 (RSP11129)
  2. 0.249 Cherry (RSP11143)
  3. 0.254 Durban Poison (RSP11014)
  4. 0.287 Blueberry Cheesecake (RSP10684)
  5. 0.287 Cbot-2019-004 (RSP11132)
  6. 0.290 Liberty Haze (RSP11000)
  7. 0.298 Blueberry Cheesecake (RSP10672)
  8. 0.303 UP Sunrise (RSP10989)
  9. 0.314 RKM-2018-003 (RSP11094)
  10. 0.322 CST (RSP11002)
  11. 0.322 Recon (RSP10755)
  12. 0.322 Cherry (RSP11142)
  13. 0.322 QUEEN JESUS (RSP10105)
  14. 0.323 RKM-2018-006 (RSP11097)
  15. 0.331 RKM-2018-027 (RSP11119)
  16. 0.338 Gold Cracker (RSP11048)
  17. 0.338 Golden Goat 2 (RSP10991)
  18. 0.340 Blueberry Cheesecake (RSP10680)
  19. 0.345 RKM-2018-031 (RSP11123)
  20. 0.346 RKM-2018-020 (RSP11112)

Most genetically distant strains (All Samples)

0 0.117 0.233 0.350 0.467
clone distance sibling distance more distant
  1. 0.450 80E (RSP11213)
  2. 0.442 80E (RSP11211)
  3. 0.442 80E (RSP11212)
  4. 0.435 Red Eye OG (RSP11190)
  5. 0.434 Abacus (RSP11266)
  6. 0.429 Chem 91 (RSP11185)
  7. 0.425 RKM-2018-026 (RSP11118)
  8. 0.423 Skywalker OG (RSP10837)
  9. 0.421 RKM-2018-013 (RSP11104)
  10. 0.421 Rugburn OG (RSP11353)
  11. 0.419 RKM-2018-012 (RSP11103)
  12. 0.418 Garlic (RSP11341)
  13. 0.417 JL yellow (RSP11075)
  14. 0.417 Kush Hemp E1 (RSP11128)
  15. 0.415 Star Dawg (RSP11352)
  16. 0.407 Feral (RSP11205)
  17. 0.407 501st OG (RSP11241)
  18. 0.406 JL 4th Gen 5 (RSP11199)
  19. 0.405 SFVxTK (RSP11072)
  20. 0.405 RKM-2018-022 (RSP11114)

Most genetically distant strains (Base Tree)

0 0.117 0.233 0.350 0.467
clone distance sibling distance more distant
  1. 0.436 RKM-2018-026 (RSP11118)
  2. 0.433 Skywalker OG (RSP10837)
  3. 0.420 RKM-2018-022 (RSP11114)
  4. 0.417 Kush Hemp E1 (RSP11128)
  5. 0.416 JL yellow (RSP11075)
  6. 0.404 Cbot-2019-006 (RSP11134)
  7. 0.403 Monoica (RSP10241)
  8. 0.401 RKM-2018-002 (RSP11093)
  9. 0.399 Santhica27 (RSP11047)
  10. 0.397 USO 31 (RSP10981)
  11. 0.393 Carmagnola (RSP11037)
  12. 0.392 RKM-2018-033 (RSP11125)
  13. 0.391 Ivory (RSP10668)
  14. 0.391 RKM-2018-019 (RSP11111)
  15. 0.387 Feral (RSP10890)
  16. 0.387 Fedora 17 (RSP10661)
  17. 0.387 Tisza (RSP11044)
  18. 0.386 KYRG-11 (RSP11051)
  19. 0.386 RKM-2018-018 (RSP11110)
  20. 0.385 Futura 75 (RSP10664)

Nearest genetic relative in Phylos dataset

Phylos Strain SRR8346788
Overlapping SNPs:
73
Concordance:
70

Nearest genetic relative in Lynch dataset

Lynch Strain SRR3495196
Overlapping SNPs:
2
Concordance:
2

Blockchain Registration Information

Transaction ID
af459809edecfd863596846fed0007102546e470351d721f2decac20aea34578
Stamping Certificate
Download PDF (852.7 KB)
SHASUM Hash
c7aab846485b67db7063ffb0f7f11d8349f6e61140889399632b53b185910065
QR code for RSP10941

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