Erez_05MAY2017

RSP 10942

Grower: DigiPath Labs

General Information

Accession Date
May 4, 2017
Reported Plant Sex
not reported

The strain rarity visualization shows how distant the strain is from the other cultivars in the Kannapedia database. The y-axis represents genetic distance, getting farther as you go up. The width of the visualization at any position along the y-axis shows how many strains there are in the database at that genetic distance. So, a common strain will have a more bottom-heavy shape, while uncommon and rare cultivars will have a visualization that is generally shifted towards the top.

Rarity: Uncommon
Most Distant Most Similar

Chemical Information

Cannabinoid and terpenoid information provided by the grower.

Cannabinoids

No information provided.

Terpenoids

No information provided.

Genetic Information

Plant Type
Type I

The bell curve in the heterozygosity visualization shows the distribution of heterozygosity levels for cannabis cultivars in the Kannapedia database. The green line shows where this particular strain fits within the distribution. Heterozygosity is associated with heterosis (aka hybrid vigor) but also leads to the production of more variable offspring. When plants have two genetically different parents, heterozygosity levels will be higher than if it has been inbred or backcrossed repeatedly.

Heterozygosity: 1.08%
Least Heterozygous Most Heterozygous

The ratio of reads mapped to Y-contigs to reads mapped to the whole Cannabis genome (Y-ratios) has been demonstrated to be strongly correlated with plant sex typing. This plot shows the distribution of Y-ratios for all samples in our database which were sequenced with the same method (panel or WGS) as this sample and where this sample falls in the distribution.

Y-Ratio Distribution: 0.0398
male female RSP10942

This chart represents the Illumina sequence coverage over the Bt/Bd allele. These are the three regions in the cannabis genome that impact THCA, CBDA, CBGA production. Coverage over the Active CBDAS gene is highly correlated with Type II and Type III plants as described by Etienne de Meijer. Coverage over the THCA gene is highly correlated with Type I and Type II plants but is anti-correlated with Type III plants. Type I plants require coverage over the inactive CBDA loci and no coverage over the Active CBDA gene. Lack of coverage over the Active CBDA and Active THCA allele are presumed to be Type IV plants (CBGA dominant). While deletions of entire THCAS and CBDAS genes are the most common Bt:Bd alleles observed, it is possible to have plants with these genes where functional expression of the enzyme is disrupted by deactivating point mutations (Kojoma et al. 2006).

Bt/Bd Allele Coverage

This chart represents the Illumina sequence coverage over the CBCA synthase gene.

CBCAS Coverage

Variants (THCAS, CBDAS, and CBCAS)

Gene HGVS.c HGVS.p Annotation Annotation Impact Contig Contig Pos Ref/Alt Var Freq
THCAS c.998C>G p.Pro333Arg missense variant moderate contig741 4416830

IGV: Start, Jump

G/C
NGS:
0.182
C90:
0.000

Variants (Select Genes of Interest)

PKSG-2a

UniProt

c.67T>A p.Phe23Ile missense variant moderate contig700 1945567

IGV: Start, Jump

A/T
NGS:
0.825
C90:
0.904
PKSG-2a

UniProt

c.31A>T p.Thr11Ser missense variant moderate contig700 1945603

IGV: Start, Jump

T/A
NGS:
0.763
C90:
0.876
PKSG-2b

UniProt

c.1152T>A p.Asn384Lys missense variant moderate contig700 1950486

IGV: Start, Jump

A/T
NGS:
0.715
C90:
0.895
PKSG-2b

UniProt

c.1132C>G p.Leu378Val missense variant moderate contig700 1950506

IGV: Start, Jump

G/C
NGS:
0.717
C90:
0.000
PKSG-2b

UniProt

c.1117A>G p.Ile373Val missense variant moderate contig700 1950521

IGV: Start, Jump

T/C
NGS:
0.807
C90:
0.981
PKSG-2b

UniProt

c.948T>G p.Asp316Glu missense variant moderate contig700 1950690

IGV: Start, Jump

A/C
NGS:
0.456
C90:
0.000
PKSG-2b

UniProt

c.945T>G p.Ser315Arg missense variant moderate contig700 1950693

IGV: Start, Jump

A/C
NGS:
0.454
C90:
0.000
PKSG-2b

UniProt

c.944G>A p.Ser315Asn missense variant moderate contig700 1950694

IGV: Start, Jump

C/T
NGS:
0.445
C90:
0.000
PKSG-2b

UniProt

c.934C>G p.His312Asp missense variant moderate contig700 1950704

IGV: Start, Jump

G/C
NGS:
0.410
C90:
0.000
PKSG-2b

UniProt

c.31A>T p.Thr11Ser missense variant moderate contig700 1951851

IGV: Start, Jump

T/A
NGS:
0.844
C90:
0.880
PKSG-2b

UniProt

c.-2_1dupATA start lost & conservative inframe insertion high contig700 1951880

IGV: Start, Jump

A/ATAT
NGS:
0.410
C90:
0.000
PKSG-4b

UniProt

c.496A>G p.Lys166Glu missense variant moderate contig700 2721177

IGV: Start, Jump

T/C
NGS:
0.651
C90:
0.684
PKSG-4b

UniProt

c.489delT p.Phe163fs frameshift variant high contig700 2721183

IGV: Start, Jump

CA/C
NGS:
0.792
C90:
0.761
PKSG-4b

UniProt

c.485A>G p.Lys162Arg missense variant moderate contig700 2721188

IGV: Start, Jump

T/C
NGS:
0.643
C90:
0.301
PKSG-4b

UniProt

c.431T>G p.Val144Gly missense variant moderate contig700 2721242

IGV: Start, Jump

A/C
NGS:
0.575
C90:
0.679
PKSG-4b

UniProt

c.352_355delACAG p.Thr118fs frameshift variant high contig700 2721317

IGV: Start, Jump

CCTGT/C
NGS:
0.529
C90:
0.000
PKSG-4b

UniProt

c.323A>G p.Glu108Gly missense variant moderate contig700 2721350

IGV: Start, Jump

T/C
NGS:
0.469
C90:
0.000
aPT4

UniProt

c.16G>A p.Val6Ile missense variant moderate contig121 2828672

IGV: Start, Jump

G/A
NGS:
0.158
C90:
0.000
aPT4

UniProt

c.97T>C p.Tyr33His missense variant moderate contig121 2828753

IGV: Start, Jump

T/C
NGS:
0.439
C90:
0.000
aPT4

UniProt

c.153A>C p.Lys51Asn missense variant moderate contig121 2828809

IGV: Start, Jump

A/C
NGS:
0.375
C90:
0.000
aPT4

UniProt

c.775delT p.Tyr259fs frameshift variant high contig121 2831380

IGV: Start, Jump

AT/A
NGS:
0.224
C90:
0.000
aPT4

UniProt

c.1168T>C p.Tyr390His missense variant moderate contig121 2833503

IGV: Start, Jump

T/C
NGS:
0.546
C90:
0.000
aPT1

UniProt

c.406A>G p.Ile136Val missense variant moderate contig121 2839605

IGV: Start, Jump

A/G
NGS:
0.579
C90:
0.761
aPT1

UniProt

c.629C>T p.Thr210Ile missense variant moderate contig121 2840237

IGV: Start, Jump

C/T
NGS:
0.561
C90:
0.598
HDS-2

UniProt

c.82_93delGTAACCGGAACT p.Val28_Thr31del conservative inframe deletion moderate contig95 1989748

IGV: Start, Jump

CGTAACCGGAACT/C
NGS:
0.805
C90:
0.000
HDS-2

UniProt

c.127T>G p.Ser43Ala missense variant moderate contig95 1989794

IGV: Start, Jump

T/G
NGS:
0.721
C90:
0.000

Nearest genetic relatives (All Samples)

0 0.058 0.117 0.175 0.233
clone distance sibling distance more distant
  1. 0.000 Alaska USA (RSP11171)
  2. 0.152 Serious Happiness (RSP10763)
  3. 0.161 Domnesia (RSP11184)
  4. 0.162 Liberty Haze (RSP11000)
  5. 0.165 Durban Poison 1 (RSP10996)
  6. 0.167 Durban Poison 1 (RSP11013)
  7. 0.171 Liberty Haze (RSP10946)
  8. 0.177 Gold Cracker (RSP11048)
  9. 0.178 Blueberry Cheesecake (RSP10684)
  10. 0.181 Durban Poison (RSP11014)
  11. 0.185 Doug s Varin (RSP11243)
  12. 0.185 Big Bud (RSP11221)
  13. 0.188 Black Jack (RSP10603)
  14. 0.189 Saint Jack (RSP11179)
  15. 0.191 Blue Dream (RSP11017)
  16. 0.192 Gold Cracker (RSP11041)
  17. 0.196 Jacks Cleaner (RSP11347)
  18. 0.201 RKM-2018-025 (RSP11117)
  19. 0.202 RKM-2018-001 (RSP11092)
  20. 0.203 RKM-2018-016 (RSP11108)

Nearest genetic relatives (Base Tree)

0 0.067 0.133 0.200 0.267
clone distance sibling distance more distant
  1. 0.166 Liberty Haze (RSP11000)
  2. 0.186 Durban Poison (RSP11014)
  3. 0.187 Blueberry Cheesecake (RSP10684)
  4. 0.193 Gold Cracker (RSP11048)
  5. 0.204 Golden Goat 2 (RSP10991)
  6. 0.204 RKM-2018-031 (RSP11123)
  7. 0.205 RKM-2018-023 (RSP11115)
  8. 0.217 RKM-2018-022 (RSP11114)
  9. 0.229 Italian Kiss (RSP11034)
  10. 0.236 UP Sunrise (RSP10989)
  11. 0.237 RKM-2018-027 (RSP11119)
  12. 0.239 RKM-2018-019 (RSP11111)
  13. 0.244 QUEEN JESUS (RSP10105)
  14. 0.244 Cbot-2019-006 (RSP11134)
  15. 0.250 RKM-2018-018 (RSP11110)
  16. 0.254 Hermaphrodite Research Sample1 (RSP11049)
  17. 0.258 Recon (RSP10755)
  18. 0.259 Pie Hoe (RSP11073)
  19. 0.262 CST (RSP11002)
  20. 0.262 RKM-2018-020 (RSP11112)

Most genetically distant strains (All Samples)

0 0.117 0.233 0.350 0.467
clone distance sibling distance more distant
  1. 0.462 Cherry Blossom (RSP11323)
  2. 0.455 Unknown- Cherry Wine - 001 (RSP11268)
  3. 0.447 Cbot-2019-005 (RSP11133)
  4. 0.443 Cherry Blossom (RSP11328)
  5. 0.432 Cherry Blossom (RSP11311)
  6. 0.431 Cherry Blossom (RSP11301)
  7. 0.422 Cherry Blossom (RSP11334)
  8. 0.417 80E (RSP11213)
  9. 0.416 Cherry Blossom (RSP11325)
  10. 0.412 Cherry Blossom (RSP11314)
  11. 0.411 Cherry Blossom (RSP11309)
  12. 0.411 Unknown- Cherry Wine - 003 (RSP11270)
  13. 0.410 Unknown- Cherry Wine - 002 (RSP11269)
  14. 0.407 Unknown- Cherry Wine - 004 (RSP11271)
  15. 0.403 Feral (RSP11205)
  16. 0.397 Cherry Blossom (RSP11306)
  17. 0.395 80E (RSP11212)
  18. 0.395 Cherry Blossom (RSP11318)
  19. 0.392 Cherry Blossom CBG (RSP11303)
  20. 0.389 80E (RSP11211)

Most genetically distant strains (Base Tree)

0 0.117 0.233 0.350 0.467
clone distance sibling distance more distant
  1. 0.465 Cbot-2019-005 (RSP11133)
  2. 0.379 Kush Hemp E1 (RSP11128)
  3. 0.372 Ivory (RSP10668)
  4. 0.367 Feral (RSP10890)
  5. 0.360 Santhica27 (RSP11047)
  6. 0.358 Monoica (RSP10241)
  7. 0.357 Carmagnola (RSP11037)
  8. 0.354 RKM-2018-026 (RSP11118)
  9. 0.350 Carmagnola (RSP10979)
  10. 0.350 USO 31 (RSP10981)
  11. 0.349 Cbot-2019-004 (RSP11132)
  12. 0.348 Tisza (RSP11044)
  13. 0.348 Lovrin (RSP10658)
  14. 0.340 JL yellow (RSP11075)
  15. 0.339 Futura 75 (RSP10664)
  16. 0.338 Jiangji (RSP10653)
  17. 0.336 KYRG-11 (RSP11051)
  18. 0.332 Kyrgyz Gold (RSP11054)
  19. 0.331 Cherry (RSP11143)
  20. 0.327 Tisza (RSP10659)

Nearest genetic relative in Phylos dataset

Phylos Strain SRR8346496
Overlapping SNPs:
71
Concordance:
68

Nearest genetic relative in Lynch dataset

Lynch Strain SRR3495164
Overlapping SNPs:
5
Concordance:
5

Blockchain Registration Information

Transaction ID
4e1c5357acbcf292819ee5350da323c382c0bb5e54cfbc97b8e5cc1224b48812
Stamping Certificate
Download PDF (854.7 KB)
SHASUM Hash
d0f60e588188b4b77472826d61b55cc79256c3147108a7ab5c6ca1938a72ea8f
QR code for RSP10942

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