Kimbo Slice

RSP 10997

Grower: DigiPath Labs

General Information

Sample Name
F20161209-03-7
Accession Date
August 28, 2017
Reported Plant Sex
Female

The strain rarity visualization shows how distant the strain is from the other cultivars in the Kannapedia database. The y-axis represents genetic distance, getting farther as you go up. The width of the visualization at any position along the y-axis shows how many strains there are in the database at that genetic distance. So, a common strain will have a more bottom-heavy shape, while uncommon and rare cultivars will have a visualization that is generally shifted towards the top.

Rarity: Rare
Most Distant Most Similar

Chemical Information

Cannabinoid and terpenoid information provided by the grower.

Cannabinoids

THC + THCA
23.1%
CBD + CBDA
0.037%
THCV + THCVA
0.%
CBC + CBCA
0.%
CBG + CBGA
0.944%
CBN + CBNA
0.053%

Terpenoids

α-Bisabolol
0.001%
Borneol
n/a
Camphene
0.004%
Carene
0.052%
Caryophyllene oxide
0.%
β-Caryophyllene
0.026%
Fenchol
n/a
Geraniol
0.001%
α-Humulene
0.024%
Limonene
0.114%
Linalool
0.01%
Myrcene
0.103%
α-Phellandrene
n/a
Terpinolene
0.317%
α-Terpineol
n/a
α-Terpinene
0.023%
γ-Terpinene
0.486%
Total Nerolidol
0.003%
Total Ocimene
0.189%
α-Pinene
0.08%
β-Pinene
0.073%

Genetic Information

Plant Type
Type I

The bell curve in the heterozygosity visualization shows the distribution of heterozygosity levels for cannabis cultivars in the Kannapedia database. The green line shows where this particular strain fits within the distribution. Heterozygosity is associated with heterosis (aka hybrid vigor) but also leads to the production of more variable offspring. When plants have two genetically different parents, heterozygosity levels will be higher than if it has been inbred or backcrossed repeatedly.

Heterozygosity: 1.1%
Least Heterozygous Most Heterozygous

The ratio of reads mapped to Y-contigs to reads mapped to the whole Cannabis genome (Y-ratios) has been demonstrated to be strongly correlated with plant sex typing. This plot shows the distribution of Y-ratios for all samples in our database which were sequenced with the same method (panel or WGS) as this sample and where this sample falls in the distribution.

Y-Ratio Distribution: 0.0480
male female RSP10997

This chart represents the Illumina sequence coverage over the Bt/Bd allele. These are the three regions in the cannabis genome that impact THCA, CBDA, CBGA production. Coverage over the Active CBDAS gene is highly correlated with Type II and Type III plants as described by Etienne de Meijer. Coverage over the THCA gene is highly correlated with Type I and Type II plants but is anti-correlated with Type III plants. Type I plants require coverage over the inactive CBDA loci and no coverage over the Active CBDA gene. Lack of coverage over the Active CBDA and Active THCA allele are presumed to be Type IV plants (CBGA dominant). While deletions of entire THCAS and CBDAS genes are the most common Bt:Bd alleles observed, it is possible to have plants with these genes where functional expression of the enzyme is disrupted by deactivating point mutations (Kojoma et al. 2006).

Bt/Bd Allele Coverage

This chart represents the Illumina sequence coverage over the CBCA synthase gene.

CBCAS Coverage

Variants (THCAS, CBDAS, and CBCAS)

THCAS c.1229G>A p.Gly410Glu missense variant moderate contig741 4416599

IGV: Start, Jump

C/T
NGS:
0.039
C90:
0.000
THCAS c.998C>G p.Pro333Arg missense variant moderate contig741 4416830

IGV: Start, Jump

G/C
NGS:
0.182
C90:
0.000

Variants (Select Genes of Interest)

PKSG-2a

UniProt

c.67T>A p.Phe23Ile missense variant moderate contig700 1945567

IGV: Start, Jump

A/T
NGS:
0.825
C90:
0.904
PKSG-2a

UniProt

c.31A>T p.Thr11Ser missense variant moderate contig700 1945603

IGV: Start, Jump

T/A
NGS:
0.763
C90:
0.876
PKSG-2b

UniProt

c.31A>T p.Thr11Ser missense variant moderate contig700 1951851

IGV: Start, Jump

T/A
NGS:
0.844
C90:
0.880
PKSG-4b

UniProt

c.535_545delATTGGAGTGGG p.Ile179fs frameshift variant high contig700 2721127

IGV: Start, Jump

CCCCACTCCAAT/C
NGS:
0.105
C90:
0.000
PKSG-4b

UniProt

c.523C>T p.His175Tyr missense variant moderate contig700 2721150

IGV: Start, Jump

G/A
NGS:
0.112
C90:
0.325
PKSG-4b

UniProt

c.489delT p.Phe163fs frameshift variant high contig700 2721183

IGV: Start, Jump

CA/C
NGS:
0.792
C90:
0.761
aPT1

UniProt

c.160A>C p.Thr54Pro missense variant moderate contig121 2835867

IGV: Start, Jump

A/C
NGS:
0.086
C90:
0.048
aPT1

UniProt

c.727G>T p.Glu243* stop gained high contig121 2841362

IGV: Start, Jump

G/T
NGS:
0.127
C90:
0.100
aPT1

UniProt

c.864C>G p.Asn288Lys missense variant moderate contig121 2842407

IGV: Start, Jump

C/G
NGS:
0.075
C90:
0.062
HDS-2

UniProt

c.82_93delGTAACCGGAACT p.Val28_Thr31del conservative inframe deletion moderate contig95 1989748

IGV: Start, Jump

CGTAACCGGAACT/C
NGS:
0.805
C90:
0.000
HDS-2

UniProt

c.127T>G p.Ser43Ala missense variant moderate contig95 1989794

IGV: Start, Jump

T/G
NGS:
0.721
C90:
0.000

Nearest genetic relatives (All Samples)

0 0.067 0.133 0.200 0.267
clone distance sibling distance more distant
  1. 0.186 The Gift (RSP10988)
  2. 0.212 RKM-2018-008 (RSP11099)
  3. 0.214 JL 4th Gen 7 (RSP11153)
  4. 0.217 Serious Happiness (RSP10763)
  5. 0.222 ILM (RSP12623)
  6. 0.230 Electra (RSP11366)
  7. 0.231 Blue Dream (RSP11004)
  8. 0.235 Sunday Driver (RSP11071)
  9. 0.236 Triangle Kush x Square Wave BX (RSP12100)
  10. 0.236 Lift (RSP11378)
  11. 0.238 Super Blue Dream (RSP11011)
  12. 0.239 Pie Hoe (RSP11073)
  13. 0.239 KYRG-151 (RSP11052)
  14. 0.239 Recon (RSP10755)
  15. 0.240 Liberty Haze (RSP11000)
  16. 0.241 Blue Dream (RSP11007)
  17. 0.241 JABBA S STASH (RSP11348)
  18. 0.242 JL Cross 26 (RSP11527)
  19. 0.242 Blue Dream (RSP11006)
  20. 0.243 Doug s Varin (RSP11243)

Nearest genetic relatives (Base Tree)

0 0.075 0.150 0.225 0.300
clone distance sibling distance more distant
  1. 0.188 The Gift (RSP10988)
  2. 0.242 Recon (RSP10755)
  3. 0.245 Pie Hoe (RSP11073)
  4. 0.254 RKM-2018-032 (RSP11124)
  5. 0.260 Liberty Haze (RSP11000)
  6. 0.265 Blueberry Cheesecake (RSP10684)
  7. 0.266 Blueberry Cheesecake (RSP10680)
  8. 0.266 KYRG-11 (RSP11051)
  9. 0.268 Tisza (RSP10659)
  10. 0.270 Skywalker OG (RSP10837)
  11. 0.270 Gold Cracker (RSP11048)
  12. 0.272 Skunk#18 (RSP11038)
  13. 0.276 RKM-2018-020 (RSP11112)
  14. 0.278 Golden Goat 2 (RSP10991)
  15. 0.279 RKM-2018-034 (RSP11126)
  16. 0.279 Hermaphrodite ResearchSample2 (RSP11050)
  17. 0.280 RKM-2018-026 (RSP11118)
  18. 0.285 Durban Poison (RSP11014)
  19. 0.289 Tygra (RSP10667)
  20. 0.290 Blue Dream (RSP11033)

Most genetically distant strains (All Samples)

0 0.117 0.233 0.350 0.467
clone distance sibling distance more distant
  1. 0.440 Cherry Blossom (RSP11318)
  2. 0.437 Cherry Blossom (RSP11323)
  3. 0.402 Unknown--Cherry Wine---001- (RSP11268)
  4. 0.396 Cherry Blossom (RSP11312)
  5. 0.394 BagSeed (RSP12627)
  6. 0.387 Cherry Blossom (RSP11328)
  7. 0.386 Cherry Blossom (RSP11300)
  8. 0.385 Unknown--Cherry Wine---002- (RSP11269)
  9. 0.383 80E (RSP11213)
  10. 0.380 Cherry Blossom (RSP11298)
  11. 0.378 JL#2 (RSP11076)
  12. 0.378 Northern Lights (RSP11501)
  13. 0.377 Cherry Blossom (RSP11274)
  14. 0.377 Chematonic -Cannatonic x Chemdawg- (RSP11394)
  15. 0.375 Northern Skunk (RSP11456)
  16. 0.374 Cherry Blossom (RSP11301)
  17. 0.374 Avidekel 05MAY2017 (RSP10938)
  18. 0.374 Cherry Blossom (RSP11308)
  19. 0.373 Chem 91 (RSP11185)
  20. 0.373 B52 (SRR14708255)

Most genetically distant strains (Base Tree)

0 0.092 0.183 0.275 0.367
clone distance sibling distance more distant
  1. 0.366 Cbot-2019-005 (RSP11133)
  2. 0.362 Cherry (RSP11142)
  3. 0.354 Blueberry Cheesecake (RSP10672)
  4. 0.351 RKM-2018-002 (RSP11093)
  5. 0.349 Cbot-2019-004 (RSP11132)
  6. 0.349 RKM-2018-028 (RSP11120)
  7. 0.346 RKM-2018-022 (RSP11114)
  8. 0.342 Cherry (RSP11143)
  9. 0.340 JL yellow (RSP11075)
  10. 0.335 Kush Hemp E1 (RSP11128)
  11. 0.332 RKM-2018-023 (RSP11115)
  12. 0.330 RKM-2018-006 (RSP11097)
  13. 0.329 Cbot-2019-001 (RSP11129)
  14. 0.329 Monoica (RSP10241)
  15. 0.326 RKM-2018-009 (RSP11100)
  16. 0.326 RKM-2018-019 (RSP11111)
  17. 0.324 Black Beauty (RSP11035)
  18. 0.323 RKM-2018-027 (RSP11119)
  19. 0.320 Cbot-2019-006 (RSP11134)
  20. 0.320 RKM-2018-018 (RSP11110)

Nearest genetic relative in Phylos dataset

Phylos Strain SRR4448408
Overlapping SNPs:
111
Concordance:
68

Nearest genetic relative in Lynch dataset

Lynch Strain SRR3495192
Overlapping SNPs:
6
Concordance:
5

Blockchain Registration Information

Transaction ID
7afa2f444d39280afefacfe1fc4d66c41a3bbc7594e0c6145790aaebc0ef1eae
Stamping Certificate
Download PDF (842.3 KB)
SHASUM Hash
6d27bad6db7d908faa240eb9daf4295fa6182fe80fabc594cb44b0d4ad25a1ed
QR code for RSP10997

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