Durban Poison #1

RSP 11013

Grower: DigiPath Labs

General Information

Sample Name
F20160801-04-15
Accession Date
September 17, 2017
Reported Plant Sex
Female

The strain rarity visualization shows how distant the strain is from the other cultivars in the Kannapedia database. The y-axis represents genetic distance, getting farther as you go up. The width of the visualization at any position along the y-axis shows how many strains there are in the database at that genetic distance. So, a common strain will have a more bottom-heavy shape, while uncommon and rare cultivars will have a visualization that is generally shifted towards the top.

Rarity: Common
Most Distant Most Similar

Chemical Information

Cannabinoid and terpenoid information provided by the grower.

Cannabinoids

THC + THCA
24.9%
CBD + CBDA
0.056%
THCV + THCVA
0.%
CBC + CBCA
0.011%
CBG + CBGA
1.031%
CBN + CBNA
0.09%

Terpenoids

α-Bisabolol
0.005%
Borneol
n/a
Camphene
0.002%
Carene
0.053%
Caryophyllene oxide
0.004%
β-Caryophyllene
0.099%
Fenchol
n/a
Geraniol
0.016%
α-Humulene
0.035%
Limonene
0.071%
Linalool
0.011%
Myrcene
0.107%
α-Phellandrene
n/a
Terpinolene
0.596%
α-Terpineol
n/a
α-Terpinene
0.036%
γ-Terpinene
0.71%
Total Nerolidol
0.006%
Total Ocimene
0.315%
α-Pinene
0.049%
β-Pinene
0.085%

Genetic Information

Plant Type
Type I

The bell curve in the heterozygosity visualization shows the distribution of heterozygosity levels for cannabis cultivars in the Kannapedia database. The green line shows where this particular strain fits within the distribution. Heterozygosity is associated with heterosis (aka hybrid vigor) but also leads to the production of more variable offspring. When plants have two genetically different parents, heterozygosity levels will be higher than if it has been inbred or backcrossed repeatedly.

Heterozygosity: 1%
Least Heterozygous Most Heterozygous

The ratio of reads mapped to Y-contigs to reads mapped to the whole Cannabis genome (Y-ratios) has been demonstrated to be strongly correlated with plant sex typing. This plot shows the distribution of Y-ratios for all samples in our database which were sequenced with the same method (panel or WGS) as this sample and where this sample falls in the distribution.

Y-Ratio Distribution: 0.0441
male female RSP11013

This chart represents the Illumina sequence coverage over the Bt/Bd allele. These are the three regions in the cannabis genome that impact THCA, CBDA, CBGA production. Coverage over the Active CBDAS gene is highly correlated with Type II and Type III plants as described by Etienne de Meijer. Coverage over the THCA gene is highly correlated with Type I and Type II plants but is anti-correlated with Type III plants. Type I plants require coverage over the inactive CBDA loci and no coverage over the Active CBDA gene. Lack of coverage over the Active CBDA and Active THCA allele are presumed to be Type IV plants (CBGA dominant). While deletions of entire THCAS and CBDAS genes are the most common Bt:Bd alleles observed, it is possible to have plants with these genes where functional expression of the enzyme is disrupted by deactivating point mutations (Kojoma et al. 2006).

Bt/Bd Allele Coverage

This chart represents the Illumina sequence coverage over the CBCA synthase gene.

CBCAS Coverage

Variants (THCAS, CBDAS, and CBCAS)

No variants to report

Variants (Select Genes of Interest)

PKSG-2a

UniProt

c.67T>A p.Phe23Ile missense variant moderate contig700 1945567

IGV: Start, Jump

A/T
NGS:
0.825
C90:
0.904
PKSG-2a

UniProt

c.31A>T p.Thr11Ser missense variant moderate contig700 1945603

IGV: Start, Jump

T/A
NGS:
0.763
C90:
0.876
aPT4

UniProt

c.1168T>C p.Tyr390His missense variant moderate contig121 2833503

IGV: Start, Jump

T/C
NGS:
0.546
C90:
0.000
aPT1

UniProt

c.629C>T p.Thr210Ile missense variant moderate contig121 2840237

IGV: Start, Jump

C/T
NGS:
0.561
C90:
0.598
HDS-2

UniProt

c.82_93delGTAACCGGAACT p.Val28_Thr31del conservative inframe deletion moderate contig95 1989748

IGV: Start, Jump

CGTAACCGGAACT/C
NGS:
0.805
C90:
0.000

Nearest genetic relatives (All Samples)

0 0.050 0.100 0.150 0.200
clone distance sibling distance more distant
  1. 0.003 Durban Poison 1 (RSP10996)
  2. 0.003 Durban Poison (RSP11226)
  3. 0.007 Durban Poison (RSP10998)
  4. 0.010 Durban Poison (RSP11014)
  5. 0.134 Black Jack (RSP10603)
  6. 0.142 Domnesia (RSP11184)
  7. 0.146 RKM-2018-025 (RSP11117)
  8. 0.146 BLACK JACK (RSP11346)
  9. 0.146 RKM-2018-016 (RSP11108)
  10. 0.148 Doug s Varin (RSP11243)
  11. 0.153 Whitey (RSP11363)
  12. 0.154 Gold Cracker (RSP11048)
  13. 0.166 Gold Cracker (RSP11041)
  14. 0.167 Erez (RSP10942)
  15. 0.170 Serious Happiness (RSP10763)
  16. 0.175 Saint Jack (RSP11179)
  17. 0.176 Blue Dream (RSP11004)
  18. 0.179 Alaska USA (RSP11171)
  19. 0.182 RKM-2018-027 (RSP11119)
  20. 0.183 Super Blue Dream (RSP11011)

Nearest genetic relatives (Base Tree)

0 0.067 0.133 0.200 0.267
clone distance sibling distance more distant
  1. 0.009 Durban Poison (RSP11014)
  2. 0.151 Gold Cracker (RSP11048)
  3. 0.176 RKM-2018-027 (RSP11119)
  4. 0.184 Liberty Haze (RSP11000)
  5. 0.189 Blueberry Cheesecake (RSP10684)
  6. 0.209 CST (RSP11002)
  7. 0.217 Golden Goat 2 (RSP10991)
  8. 0.224 UP Sunrise (RSP10989)
  9. 0.228 RKM-2018-003 (RSP11094)
  10. 0.233 RKM-2018-020 (RSP11112)
  11. 0.233 RKM-2018-029 (RSP11121)
  12. 0.234 RKM-2018-023 (RSP11115)
  13. 0.238 Hermaphrodite ResearchSample2 (RSP11050)
  14. 0.238 Cbot-2019-006 (RSP11134)
  15. 0.239 Blue Dream (RSP11033)
  16. 0.242 Pie Hoe (RSP11073)
  17. 0.246 RKM-2018-006 (RSP11097)
  18. 0.247 RKM-2018-033 (RSP11125)
  19. 0.247 Skunk 18 (RSP11038)
  20. 0.247 RKM-2018-005 (RSP11096)

Most genetically distant strains (All Samples)

0 0.108 0.217 0.325 0.433
clone distance sibling distance more distant
  1. 0.401 80E (RSP11213)
  2. 0.397 Cherry Blossom (RSP11323)
  3. 0.385 80E (RSP11212)
  4. 0.380 Cherry Blossom (RSP11318)
  5. 0.379 80E (RSP11211)
  6. 0.373 Cherry Blossom (RSP11314)
  7. 0.365 CS (RSP11208)
  8. 0.363 Feral (RSP11205)
  9. 0.363 Kush Hemp E1 (RSP11128)
  10. 0.358 Cbot-2019-005 (RSP11133)
  11. 0.357 Cherry Blossom (RSP11333)
  12. 0.349 Cherry Blossom (RSP11306)
  13. 0.349 Feral (RSP11206)
  14. 0.347 Cherry Blossom (RSP11311)
  15. 0.343 JL yellow (RSP11075)
  16. 0.338 Carmaleonte (RSP11207)
  17. 0.337 Cherry Blossom (RSP11328)
  18. 0.335 Arcata Trainwreck (RSP11176)
  19. 0.334 Ivory (RSP10668)
  20. 0.332 Chem 91 (RSP11185)

Most genetically distant strains (Base Tree)

0 0.092 0.183 0.275 0.367
clone distance sibling distance more distant
  1. 0.362 Kush Hemp E1 (RSP11128)
  2. 0.361 Cbot-2019-005 (RSP11133)
  3. 0.342 Ivory (RSP10668)
  4. 0.342 JL yellow (RSP11075)
  5. 0.333 Monoica (RSP10241)
  6. 0.331 Carmagnola (RSP11037)
  7. 0.326 Santhica27 (RSP11047)
  8. 0.323 Feral (RSP10890)
  9. 0.320 Kyrgyz Gold (RSP11054)
  10. 0.316 KYRG-11 (RSP11051)
  11. 0.313 Fedora 17 (RSP10661)
  12. 0.310 RKM-2018-026 (RSP11118)
  13. 0.307 USO 31 (RSP10981)
  14. 0.304 Lovrin (RSP10658)
  15. 0.301 Futura 75 (RSP10664)
  16. 0.299 RKM-2018-034 (RSP11126)
  17. 0.297 Tisza (RSP10659)
  18. 0.296 Tisza (RSP11044)
  19. 0.294 Carmagnola (RSP10979)
  20. 0.293 Cbot-2019-001 (RSP11129)

Nearest genetic relative in Phylos dataset

Phylos Strain SRR8346689
Overlapping SNPs:
68
Concordance:
67

Nearest genetic relative in Lynch dataset

Lynch Strain SRR3495285
Overlapping SNPs:
6
Concordance:
6

Blockchain Registration Information

Transaction ID
042179e973bd8732d672517c8108ff73541eb43d5b8617949cf87ad1aa0429f7
Stamping Certificate
Download PDF (844.3 KB)
SHASUM Hash
1b68f4b39dd03f70ed5c42be7143c391b8ec36e3e817676b95a5787c608d7720
QR code for RSP11013

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