Durban Poison

RSP 11014

Grower: DigiPath Labs

General Information

Sample Name
F20170411-04-1
Accession Date
September 17, 2017
Reported Plant Sex
Female
DNA Extracted From
Unknown

The strain rarity visualization shows how distant the strain is from the other cultivars in the Kannapedia database. The y-axis represents genetic distance, getting farther as you go up. The width of the visualization at any position along the y-axis shows how many strains there are in the database at that genetic distance. So, a common strain will have a more bottom-heavy shape, while uncommon and rare cultivars will have a visualization that is generally shifted towards the top.

Rarity: Uncommon
Most Distant Most Similar

Chemical Information

Cannabinoid and terpenoid information provided by the grower.

Cannabinoids

THC + THCA
25.7%
CBD + CBDA
0.052%
THCV + THCVA
0.%
CBC + CBCA
0.046%
CBG + CBGA
0.766%
CBN + CBNA
0.355%

Terpenoids

α-Bisabolol
0.026%
Borneol
n/a
Camphene
0.014%
Carene
0.214%
Caryophyllene oxide
0.015%
β-Caryophyllene
0.337%
Fenchol
n/a
Geraniol
0.014%
α-Humulene
0.147%
Limonene
0.656%
Linalool
0.022%
Myrcene
2.163%
α-Phellandrene
n/a
Terpinolene
6.619%
α-Terpineol
n/a
α-Terpinene
0.297%
γ-Terpinene
5.543%
Total Nerolidol
0.044%
Total Ocimene
2.885%
α-Pinene
0.485%
β-Pinene
1.065%

Genetic Information

Plant Type
Type I

The bell curve in the heterozygosity visualization shows the distribution of heterozygosity levels for cannabis cultivars in the Kannapedia database. The green line shows where this particular strain fits within the distribution. Heterozygosity is associated with heterosis (aka hybrid vigor) but also leads to the production of more variable offspring. When plants have two genetically different parents, heterozygosity levels will be higher than if it has been inbred or backcrossed repeatedly.

Heterozygosity: 0.93%
Least Heterozygous Most Heterozygous

The ratio of reads mapped to Y-contigs to reads mapped to the whole Cannabis genome (Y-ratios) has been demonstrated to be strongly correlated with plant sex typing. This plot shows the distribution of Y-ratios for all samples in our database which were sequenced with the same method (panel or WGS) as this sample and where this sample falls in the distribution.

Y-Ratio Distribution: 0.0440
male female RSP11014

This chart represents the Illumina sequence coverage over the Bt/Bd allele. These are the three regions in the cannabis genome that impact THCA, CBDA, CBGA production. Coverage over the Active CBDAS gene is highly correlated with Type II and Type III plants as described by Etienne de Meijer. Coverage over the THCA gene is highly correlated with Type I and Type II plants but is anti-correlated with Type III plants. Type I plants require coverage over the inactive CBDA loci and no coverage over the Active CBDA gene. Lack of coverage over the Active CBDA and Active THCA allele are presumed to be Type IV plants (CBGA dominant). While deletions of entire THCAS and CBDAS genes are the most common Bt:Bd alleles observed, it is possible to have plants with these genes where functional expression of the enzyme is disrupted by deactivating point mutations (Kojoma et al. 2006).

Bt/Bd Allele Coverage

This chart represents the Illumina sequence coverage over the CBCA synthase gene.

CBCAS Coverage

Variants (THCAS, CBDAS, and CBCAS)

No variants to report

Variants (Select Genes of Interest)

PKSG-2a

UniProt

c.67T>A p.Phe23Ile missense variant moderate contig700 1945567

IGV: Start, Jump

A/T
NGS:
0.825
C90:
0.904
PKSG-2a

UniProt

c.31A>T p.Thr11Ser missense variant moderate contig700 1945603

IGV: Start, Jump

T/A
NGS:
0.763
C90:
0.876
PKSG-2b

UniProt

c.1152T>A p.Asn384Lys missense variant moderate contig700 1950486

IGV: Start, Jump

A/T
NGS:
0.715
C90:
0.895
PKSG-2b

UniProt

c.1132C>G p.Leu378Val missense variant moderate contig700 1950506

IGV: Start, Jump

G/C
NGS:
0.717
C90:
0.000
PKSG-2b

UniProt

c.1117A>G p.Ile373Val missense variant moderate contig700 1950521

IGV: Start, Jump

T/C
NGS:
0.807
C90:
0.981
PKSG-4b

UniProt

c.496A>G p.Lys166Glu missense variant moderate contig700 2721177

IGV: Start, Jump

T/C
NGS:
0.651
C90:
0.684
PKSG-4b

UniProt

c.489delT p.Phe163fs frameshift variant high contig700 2721183

IGV: Start, Jump

CA/C
NGS:
0.792
C90:
0.761
PKSG-4b

UniProt

c.485A>G p.Lys162Arg missense variant moderate contig700 2721188

IGV: Start, Jump

T/C
NGS:
0.643
C90:
0.301
aPT1

UniProt

c.406A>G p.Ile136Val missense variant moderate contig121 2839605

IGV: Start, Jump

A/G
NGS:
0.579
C90:
0.761

Nearest genetic relatives (All Samples)

0 0.050 0.100 0.150 0.200
clone distance sibling distance more distant
  1. 0.006 Durban Poison (RSP11226)
  2. 0.007 Durban Poison (RSP10998)
  3. 0.008 Durban Poison #1 (RSP11013)
  4. 0.011 Durban Poison #1 (RSP10996)
  5. 0.139 Black Jack (RSP10603)
  6. 0.142 Domnesia (RSP11184)
  7. 0.150 RKM-2018-025 (RSP11117)
  8. 0.151 RKM-2018-016 (RSP11108)
  9. 0.155 Lift (RSP11378)
  10. 0.156 BLACK JACK (RSP11346)
  11. 0.164 UnObtanium (RSP11611)
  12. 0.169 Gold Cracker (RSP11048)
  13. 0.171 Electra (RSP11366)
  14. 0.173 Doug s Varin (RSP11243)
  15. 0.175 Serious Happiness (RSP10763)
  16. 0.182 Gold Cracker (RSP11041)
  17. 0.184 Saint Jack (RSP11179)
  18. 0.185 Trump x Trump (RSP11466)
  19. 0.187 RKM-2018-027 (RSP11119)
  20. 0.188 Blue Dream (RSP11004)

Most genetically distant strains (All Samples)

0 0.108 0.217 0.325 0.433
clone distance sibling distance more distant
  1. 0.419 Cherry Blossom (RSP11323)
  2. 0.418 80E (RSP11213)
  3. 0.408 80E (RSP11211)
  4. 0.399 80E (RSP11212)
  5. 0.396 CS (RSP11208)
  6. 0.392 Cherry Blossom (RSP11314)
  7. 0.389 Feral (RSP11205)
  8. 0.383 Feral (RSP11206)
  9. 0.382 Cherry Blossom (RSP11318)
  10. 0.379 R1in136 (SRR14708227)
  11. 0.378 R1in136 (SRR14708226)
  12. 0.377 Cherry Blossom (RSP11328)
  13. 0.377 Feral (RSP10891)
  14. 0.377 Feral (RSP10890)
  15. 0.375 Cherry Blossom (RSP11317)
  16. 0.374 Cherry Blossom (RSP11333)
  17. 0.370 Cherry Blossom (RSP11306)
  18. 0.368 IUP2 (SRR14708257)
  19. 0.368 XBL1 (SRR14708207)
  20. 0.367 Cherry Blossom (RSP11311)

Nearest genetic relative in Phylos dataset

Phylos Strain SRR8346957
Overlapping SNPs:
77
Concordance:
76

Nearest genetic relative in Lynch dataset

Lynch Strain SRR3495198
Overlapping SNPs:
10
Concordance:
10

Blockchain Registration Information

Transaction ID
d6e4fab293bf94f923a0f6cac001ab7b1ced8f01640f20139ed20b6054d384f8
Stamping Certificate
Download PDF (854.2 KB)
SHASUM Hash
5b0f2a198fc77e26575b920b088265567e85e94048617010b1b5f7d301e23e00
QR code for RSP11014

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