Tisza

RSP 11045

Grower: CSU

General Information

Sample Name
Tisza-1
Accession Date
January 7, 2018
Reported Plant Sex
Male

The strain rarity visualization shows how distant the strain is from the other cultivars in the Kannapedia database. The y-axis represents genetic distance, getting farther as you go up. The width of the visualization at any position along the y-axis shows how many strains there are in the database at that genetic distance. So, a common strain will have a more bottom-heavy shape, while uncommon and rare cultivars will have a visualization that is generally shifted towards the top.

Rarity: Uncommon
Most Distant Most Similar

Chemical Information

Cannabinoid and terpenoid information provided by the grower.

Cannabinoids

No information provided.

Terpenoids

No information provided.

Genetic Information

Plant Type
Type II

The bell curve in the heterozygosity visualization shows the distribution of heterozygosity levels for cannabis cultivars in the Kannapedia database. The green line shows where this particular strain fits within the distribution. Heterozygosity is associated with heterosis (aka hybrid vigor) but also leads to the production of more variable offspring. When plants have two genetically different parents, heterozygosity levels will be higher than if it has been inbred or backcrossed repeatedly.

Heterozygosity: 1.31%
Least Heterozygous Most Heterozygous

The ratio of reads mapped to Y-contigs to reads mapped to the whole Cannabis genome (Y-ratios) has been demonstrated to be strongly correlated with plant sex typing. This plot shows the distribution of Y-ratios for all samples in our database which were sequenced with the same method (panel or WGS) as this sample and where this sample falls in the distribution.

Y-Ratio Distribution: 0.0970
male female RSP11045

This chart represents the Illumina sequence coverage over the Bt/Bd allele. These are the three regions in the cannabis genome that impact THCA, CBDA, CBGA production. Coverage over the Active CBDAS gene is highly correlated with Type II and Type III plants as described by Etienne de Meijer. Coverage over the THCA gene is highly correlated with Type I and Type II plants but is anti-correlated with Type III plants. Type I plants require coverage over the inactive CBDA loci and no coverage over the Active CBDA gene. Lack of coverage over the Active CBDA and Active THCA allele are presumed to be Type IV plants (CBGA dominant). While deletions of entire THCAS and CBDAS genes are the most common Bt:Bd alleles observed, it is possible to have plants with these genes where functional expression of the enzyme is disrupted by deactivating point mutations (Kojoma et al. 2006).

Bt/Bd Allele Coverage

This chart represents the Illumina sequence coverage over the CBCA synthase gene.

CBCAS Coverage

Variants (THCAS, CBDAS, and CBCAS)

Gene HGVS.c HGVS.p Annotation Annotation Impact Contig Contig Pos Ref/Alt Var Freq
THCAS c.187A>C p.Ile63Leu missense variant moderate contig741 4417641

IGV: Start, Jump

T/G
NGS:
0.059
C90:
0.679

Variants (Select Genes of Interest)

PKSG-2a

UniProt

c.-22_2delTAAAAATTATAATAATAATAATAT p.Met1del start lost & conservative inframe deletion high contig700 1945631

IGV: Start, Jump

CATATTATTATTATTATAATTTTTA/C
NGS:
0.013
C90:
0.000
PKSG-2b

UniProt

c.1152T>A p.Asn384Lys missense variant moderate contig700 1950486

IGV: Start, Jump

A/T
NGS:
0.715
C90:
0.895
PKSG-2b

UniProt

c.948T>G p.Asp316Glu missense variant moderate contig700 1950690

IGV: Start, Jump

A/C
NGS:
0.456
C90:
0.000
PKSG-2b

UniProt

c.945T>G p.Ser315Arg missense variant moderate contig700 1950693

IGV: Start, Jump

A/C
NGS:
0.454
C90:
0.000
PKSG-2b

UniProt

c.944G>A p.Ser315Asn missense variant moderate contig700 1950694

IGV: Start, Jump

C/T
NGS:
0.445
C90:
0.000
PKSG-2b

UniProt

c.934C>G p.His312Asp missense variant moderate contig700 1950704

IGV: Start, Jump

G/C
NGS:
0.410
C90:
0.000
PKSG-4b

UniProt

c.526T>C p.Trp176Arg missense variant moderate contig700 2721147

IGV: Start, Jump

A/G
NGS:
0.037
C90:
0.000
aPT4

UniProt

c.1168T>C p.Tyr390His missense variant moderate contig121 2833503

IGV: Start, Jump

T/C
NGS:
0.546
C90:
0.000
aPT1

UniProt

c.160A>C p.Thr54Pro missense variant moderate contig121 2835867

IGV: Start, Jump

A/C
NGS:
0.086
C90:
0.048
aPT1

UniProt

c.670T>A p.Ser224Thr missense variant moderate contig121 2840278

IGV: Start, Jump

T/A
NGS:
0.077
C90:
0.000
HDS-2

UniProt

c.82_93delGTAACCGGAACT p.Val28_Thr31del conservative inframe deletion moderate contig95 1989748

IGV: Start, Jump

CGTAACCGGAACT/C
NGS:
0.805
C90:
0.000
HDS-2

UniProt

c.127T>G p.Ser43Ala missense variant moderate contig95 1989794

IGV: Start, Jump

T/G
NGS:
0.721
C90:
0.000

Nearest genetic relatives (All Samples)

0 0.058 0.117 0.175 0.233
clone distance sibling distance more distant
  1. 0.005 Tisza (RSP11044)
  2. 0.197 Carmagnola (RSP10978)
  3. 0.203 Tiborszallasie (RSP11210)
  4. 0.206 Carmagnola (RSP10979)
  5. 0.207 Carmagnola (RSP10976)
  6. 0.207 Tisza (RSP10659)
  7. 0.208 Carmagnola (RSP10982)
  8. 0.208 Santhica 27 (RSP10665)
  9. 0.212 Tygra (RSP10667)
  10. 0.212 KYRG-151 (RSP11052)
  11. 0.213 Fedora 17 (RSP10661)
  12. 0.213 Futura 75 (RSP10664)
  13. 0.215 Carmagnola (RSP11039)
  14. 0.217 Diana (RSP10235)
  15. 0.217 Carmagnola (RSP11037)
  16. 0.218 Carmagnola (RSP11202)
  17. 0.218 Carmagnola USO 31 (RSP11204)
  18. 0.220 Feral (RSP10891)
  19. 0.223 KYRG-21 (RSP11053)
  20. 0.226 Lovrin (RSP10658)

Nearest genetic relatives (Base Tree)

0 0.075 0.150 0.225 0.300
clone distance sibling distance more distant
  1. 0.004 Tisza (RSP11044)
  2. 0.202 Tisza (RSP10659)
  3. 0.210 Carmagnola (RSP10979)
  4. 0.211 Futura 75 (RSP10664)
  5. 0.211 Fedora 17 (RSP10661)
  6. 0.216 Tygra (RSP10667)
  7. 0.221 Carmagnola (RSP11037)
  8. 0.227 Lovrin (RSP10658)
  9. 0.228 Feral (RSP10890)
  10. 0.231 Monoica (RSP10241)
  11. 0.233 Santhica27 (RSP11047)
  12. 0.234 USO 31 (RSP10981)
  13. 0.238 KYRG-11 (RSP11051)
  14. 0.246 Jiangji (RSP10653)
  15. 0.247 Ivory (RSP10668)
  16. 0.256 Kyrgyz Gold (RSP11054)
  17. 0.277 Recon (RSP10755)
  18. 0.282 RKM-2018-029 (RSP11121)
  19. 0.287 Kimbo Slice (RSP10997)
  20. 0.290 Hermaphrodite ResearchSample2 (RSP11050)

Most genetically distant strains (All Samples)

0 0.117 0.233 0.350 0.467
clone distance sibling distance more distant
  1. 0.462 Cherry Blossom (RSP11323)
  2. 0.447 Cherry Blossom (RSP11318)
  3. 0.427 Cherry Blossom (RSP11311)
  4. 0.425 Cherry Blossom (RSP11334)
  5. 0.422 Unknown- Cherry Wine - 001 (RSP11268)
  6. 0.422 Cherry Blossom (RSP11331)
  7. 0.421 Cherry Blossom (RSP11312)
  8. 0.421 Cherry Blossom (RSP11328)
  9. 0.415 Cherry Blossom (RSP11325)
  10. 0.414 Cherry Blossom (RSP11298)
  11. 0.411 Cherry Blossom (RSP11301)
  12. 0.411 Cherry Blossom (RSP11308)
  13. 0.408 Cherry Blossom (RSP11335)
  14. 0.407 Cherry Blossom (RSP11300)
  15. 0.407 Cherry Blossom (RSP11299)
  16. 0.401 Cherry Blossom (RSP11333)
  17. 0.401 Cherry Blossom (RSP11302)
  18. 0.397 Cherry Blossom (RSP11324)
  19. 0.397 Wife (RSP11148)
  20. 0.397 Cherry Blossom (RSP11306)

Most genetically distant strains (Base Tree)

0 0.100 0.200 0.300 0.400
clone distance sibling distance more distant
  1. 0.389 Cbot-2019-005 (RSP11133)
  2. 0.366 Cbot-2019-001 (RSP11129)
  3. 0.359 RKM-2018-006 (RSP11097)
  4. 0.356 RKM-2018-002 (RSP11093)
  5. 0.356 Skunk 18 (RSP11038)
  6. 0.354 Kush Hemp E1 (RSP11128)
  7. 0.354 RKM-2018-028 (RSP11120)
  8. 0.354 RKM-2018-003 (RSP11094)
  9. 0.346 RKM-2018-018 (RSP11110)
  10. 0.345 RKM-2018-022 (RSP11114)
  11. 0.343 Cbot-2019-004 (RSP11132)
  12. 0.343 Cherry (RSP11143)
  13. 0.338 JL yellow (RSP11075)
  14. 0.336 RKM-2018-027 (RSP11119)
  15. 0.335 RKM-2018-032 (RSP11124)
  16. 0.335 Cherry (RSP11142)
  17. 0.335 Blueberry Cheesecake (RSP10672)
  18. 0.334 Sour Raspberry (RSP10551)
  19. 0.333 Hermaphrodite Research Sample1 (RSP11049)
  20. 0.332 RKM-2018-023 (RSP11115)

Nearest genetic relative in Phylos dataset

Phylos Strain SRR4448767
Overlapping SNPs:
86
Concordance:
54

Nearest genetic relative in Lynch dataset

Lynch Strain SRR3495166
Overlapping SNPs:
2
Concordance:
2

Blockchain Registration Information

Transaction ID
1b87fc1258bfe7fd4a1c85de5db5205a7da1e08e8b6f44d315b7ed2bbfbed4ac
Stamping Certificate
Download PDF (849.1 KB)
SHASUM Hash
5e26a33684f8c0f347c660c383eec19d796a8b7b315caa614c445137c74c5052
QR code for RSP11045

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