Hermaphrodite_Research_Sample1

RSP 11049

Grower: Iron Laboratories

General Information

Accession Date
January 7, 2018
Reported Plant Sex
not reported
DNA Extracted From
Stem

The strain rarity visualization shows how distant the strain is from the other cultivars in the Kannapedia database. The y-axis represents genetic distance, getting farther as you go up. The width of the visualization at any position along the y-axis shows how many strains there are in the database at that genetic distance. So, a common strain will have a more bottom-heavy shape, while uncommon and rare cultivars will have a visualization that is generally shifted towards the top.

Rarity: Uncommon
Most Distant Most Similar

Chemical Information

Cannabinoid and terpenoid information provided by the grower.

Cannabinoids

No information provided.

Terpenoids

No information provided.

Genetic Information

Plant Type
Type II

The bell curve in the heterozygosity visualization shows the distribution of heterozygosity levels for cannabis cultivars in the Kannapedia database. The green line shows where this particular strain fits within the distribution. Heterozygosity is associated with heterosis (aka hybrid vigor) but also leads to the production of more variable offspring. When plants have two genetically different parents, heterozygosity levels will be higher than if it has been inbred or backcrossed repeatedly.

Heterozygosity: 0.79%
Least Heterozygous Most Heterozygous

The ratio of reads mapped to Y-contigs to reads mapped to the whole Cannabis genome (Y-ratios) has been demonstrated to be strongly correlated with plant sex typing. This plot shows the distribution of Y-ratios for all samples in our database which were sequenced with the same method (panel or WGS) as this sample and where this sample falls in the distribution.

Y-Ratio Distribution: 0.0445
male female RSP11049

This chart represents the Illumina sequence coverage over the Bt/Bd allele. These are the three regions in the cannabis genome that impact THCA, CBDA, CBGA production. Coverage over the Active CBDAS gene is highly correlated with Type II and Type III plants as described by Etienne de Meijer. Coverage over the THCA gene is highly correlated with Type I and Type II plants but is anti-correlated with Type III plants. Type I plants require coverage over the inactive CBDA loci and no coverage over the Active CBDA gene. Lack of coverage over the Active CBDA and Active THCA allele are presumed to be Type IV plants (CBGA dominant). While deletions of entire THCAS and CBDAS genes are the most common Bt:Bd alleles observed, it is possible to have plants with these genes where functional expression of the enzyme is disrupted by deactivating point mutations (Kojoma et al. 2006).

Bt/Bd Allele Coverage

This chart represents the Illumina sequence coverage over the CBCA synthase gene.

CBCAS Coverage

Variants (THCAS, CBDAS, and CBCAS)

No variants to report

Variants (Select Genes of Interest)

PKSG-2a

UniProt

c.67T>A p.Phe23Ile missense variant moderate contig700 1945567

IGV: Start, Jump

A/T
NGS:
0.825
C90:
0.904
PKSG-2a

UniProt

c.31A>T p.Thr11Ser missense variant moderate contig700 1945603

IGV: Start, Jump

T/A
NGS:
0.763
C90:
0.876
PKSG-2b

UniProt

c.1152T>A p.Asn384Lys missense variant moderate contig700 1950486

IGV: Start, Jump

A/T
NGS:
0.715
C90:
0.895
PKSG-2b

UniProt

c.1132C>G p.Leu378Val missense variant moderate contig700 1950506

IGV: Start, Jump

G/C
NGS:
0.717
C90:
0.000
PKSG-2b

UniProt

c.1117A>G p.Ile373Val missense variant moderate contig700 1950521

IGV: Start, Jump

T/C
NGS:
0.807
C90:
0.981
PKSG-2b

UniProt

c.31A>T p.Thr11Ser missense variant moderate contig700 1951851

IGV: Start, Jump

T/A
NGS:
0.844
C90:
0.880
PKSG-4b

UniProt

c.496A>G p.Lys166Glu missense variant moderate contig700 2721177

IGV: Start, Jump

T/C
NGS:
0.651
C90:
0.684
PKSG-4b

UniProt

c.489delT p.Phe163fs frameshift variant high contig700 2721183

IGV: Start, Jump

CA/C
NGS:
0.792
C90:
0.761
PKSG-4b

UniProt

c.485A>G p.Lys162Arg missense variant moderate contig700 2721188

IGV: Start, Jump

T/C
NGS:
0.643
C90:
0.301
PKSG-4b

UniProt

c.431T>G p.Val144Gly missense variant moderate contig700 2721242

IGV: Start, Jump

A/C
NGS:
0.575
C90:
0.679
PKSG-4b

UniProt

c.419A>G p.Asp140Gly missense variant moderate contig700 2721254

IGV: Start, Jump

T/C
NGS:
0.430
C90:
0.440
aPT1

UniProt

c.406A>G p.Ile136Val missense variant moderate contig121 2839605

IGV: Start, Jump

A/G
NGS:
0.579
C90:
0.761
HDS-2

UniProt

c.82_93delGTAACCGGAACT p.Val28_Thr31del conservative inframe deletion moderate contig95 1989748

IGV: Start, Jump

CGTAACCGGAACT/C
NGS:
0.805
C90:
0.000
HDS-2

UniProt

c.127T>G p.Ser43Ala missense variant moderate contig95 1989794

IGV: Start, Jump

T/G
NGS:
0.721
C90:
0.000

Nearest genetic relatives (All Samples)

0 0.058 0.117 0.175 0.233
clone distance sibling distance more distant
  1. 0.004 Hermaphrodite Research Sample1 (RSP11042)
  2. 0.005 Bordello (RSP11228)
  3. 0.148 RKM-2018-018 (RSP11110)
  4. 0.169 Blue Dream (RSP11009)
  5. 0.176 Blue Dream (RSP11017)
  6. 0.178 Blue Dream (RSP11010)
  7. 0.190 Blue Dream (RSP11008)
  8. 0.190 Serious Happiness (RSP10763)
  9. 0.190 Blue Dream (RSP11004)
  10. 0.190 Blue Dream (RSP11007)
  11. 0.191 TI (RSP11149)
  12. 0.191 Sour D (RSP11343)
  13. 0.197 Rest (RSP11377)
  14. 0.199 Blue Dream (RSP11006)
  15. 0.200 Super Blue Dream (RSP11011)
  16. 0.201 Blue Dream (RSP11012)
  17. 0.206 JL Cross 13 (RSP11514)
  18. 0.206 Domnesia (RSP11184)
  19. 0.207 East Coast Sour Diesel (RSP10243)
  20. 0.209 OR 05MAY2017 (RSP10940)

Most genetically distant strains (All Samples)

0 0.125 0.250 0.375 0.500
clone distance sibling distance more distant
  1. 0.481 Cherry Blossom (RSP11311)
  2. 0.478 Cherry Blossom (RSP11328)
  3. 0.453 Cherry Blossom (RSP11314)
  4. 0.447 Cherry Blossom (RSP11334)
  5. 0.445 Unknown--Cherry Wine---001- (RSP11268)
  6. 0.443 Cherry Blossom (RSP11300)
  7. 0.438 Cherry Blossom (RSP11309)
  8. 0.437 Tiger Tail -30- (RSP11484)
  9. 0.431 Unknown--Cherry Wine---003- (RSP11270)
  10. 0.431 Cherry Blossom (RSP11312)
  11. 0.431 Unknown--Cherry Wine---002- (RSP11269)
  12. 0.431 80E (RSP11213)
  13. 0.430 Cherry Blossom (RSP11333)
  14. 0.430 Cherry Blossom (RSP11298)
  15. 0.427 Northern Skunk (RSP11456)
  16. 0.426 Cherry Blossom (RSP11324)
  17. 0.421 Cherry Blossom (RSP11317)
  18. 0.420 JL 3rd Gen Father (RSP11196)
  19. 0.416 80E (RSP11211)
  20. 0.415 80E (RSP11212)

Nearest genetic relative in Phylos dataset

Phylos Strain SRR8347003
Overlapping SNPs:
117
Concordance:
110

Nearest genetic relative in Lynch dataset

Lynch Strain SRR3495250
Overlapping SNPs:
3
Concordance:
3

Blockchain Registration Information

Transaction ID
9c948f694a4754e3d1579e56324a993e42c45505ad943a92a8a24a509dfa5eca
Stamping Certificate
Download PDF (862.5 KB)
SHASUM Hash
c279b7f1ca72f05cdc47f99df7715efae539334ae12d575bb74de8ad3221e19b
QR code for RSP11049

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