RKM-2018-008

RSP 11099

Grower: R-Kiem Seeds

General Information

Accession Date
September 6, 2018
Reported Plant Sex
Female

The strain rarity visualization shows how distant the strain is from the other cultivars in the Kannapedia database. The y-axis represents genetic distance, getting farther as you go up. The width of the visualization at any position along the y-axis shows how many strains there are in the database at that genetic distance. So, a common strain will have a more bottom-heavy shape, while uncommon and rare cultivars will have a visualization that is generally shifted towards the top.

Rarity: Uncommon
Most Distant Most Similar

Chemical Information

Cannabinoid and terpenoid information provided by the grower.

Cannabinoids

No information provided.

Terpenoids

No information provided.

Genetic Information

Plant Type
Type I

The bell curve in the heterozygosity visualization shows the distribution of heterozygosity levels for cannabis cultivars in the Kannapedia database. The green line shows where this particular strain fits within the distribution. Heterozygosity is associated with heterosis (aka hybrid vigor) but also leads to the production of more variable offspring. When plants have two genetically different parents, heterozygosity levels will be higher than if it has been inbred or backcrossed repeatedly.

Heterozygosity: 1%
Least Heterozygous Most Heterozygous

The ratio of reads mapped to Y-contigs to reads mapped to the whole Cannabis genome (Y-ratios) has been demonstrated to be strongly correlated with plant sex typing. This plot shows the distribution of Y-ratios for all samples in our database which were sequenced with the same method (panel or WGS) as this sample and where this sample falls in the distribution.

Y-Ratio Distribution: 0.0411
male female RSP11099

This chart represents the Illumina sequence coverage over the Bt/Bd allele. These are the three regions in the cannabis genome that impact THCA, CBDA, CBGA production. Coverage over the Active CBDAS gene is highly correlated with Type II and Type III plants as described by Etienne de Meijer. Coverage over the THCA gene is highly correlated with Type I and Type II plants but is anti-correlated with Type III plants. Type I plants require coverage over the inactive CBDA loci and no coverage over the Active CBDA gene. Lack of coverage over the Active CBDA and Active THCA allele are presumed to be Type IV plants (CBGA dominant). While deletions of entire THCAS and CBDAS genes are the most common Bt:Bd alleles observed, it is possible to have plants with these genes where functional expression of the enzyme is disrupted by deactivating point mutations (Kojoma et al. 2006).

Bt/Bd Allele Coverage

This chart represents the Illumina sequence coverage over the CBCA synthase gene.

CBCAS Coverage

Variants (THCAS, CBDAS, and CBCAS)

Gene HGVS.c HGVS.p Annotation Annotation Impact Contig Contig Pos Ref/Alt Var Freq
THCAS c.998C>G p.Pro333Arg missense variant moderate contig741 4416830

IGV: Start, Jump

G/C
NGS:
0.182
C90:
0.000

Variants (Select Genes of Interest)

PKSG-2a

UniProt

c.67T>A p.Phe23Ile missense variant moderate contig700 1945567

IGV: Start, Jump

A/T
NGS:
0.825
C90:
0.904
PKSG-2b

UniProt

c.1152T>A p.Asn384Lys missense variant moderate contig700 1950486

IGV: Start, Jump

A/T
NGS:
0.715
C90:
0.895
PKSG-2b

UniProt

c.1132C>G p.Leu378Val missense variant moderate contig700 1950506

IGV: Start, Jump

G/C
NGS:
0.717
C90:
0.000
PKSG-2b

UniProt

c.1124G>C p.Arg375Thr missense variant moderate contig700 1950514

IGV: Start, Jump

C/G
NGS:
0.015
C90:
0.000
PKSG-2b

UniProt

c.1117A>G p.Ile373Val missense variant moderate contig700 1950521

IGV: Start, Jump

T/C
NGS:
0.807
C90:
0.981
PKSG-2b

UniProt

c.1105C>G p.Pro369Ala missense variant moderate contig700 1950533

IGV: Start, Jump

G/C
NGS:
0.013
C90:
0.000
PKSG-2b

UniProt

c.995C>T p.Ser332Phe missense variant moderate contig700 1950643

IGV: Start, Jump

G/A
NGS:
0.042
C90:
0.000
PKSG-2b

UniProt

c.948T>G p.Asp316Glu missense variant moderate contig700 1950690

IGV: Start, Jump

A/C
NGS:
0.456
C90:
0.000
PKSG-2b

UniProt

c.945T>G p.Ser315Arg missense variant moderate contig700 1950693

IGV: Start, Jump

A/C
NGS:
0.454
C90:
0.000
PKSG-2b

UniProt

c.944G>A p.Ser315Asn missense variant moderate contig700 1950694

IGV: Start, Jump

C/T
NGS:
0.445
C90:
0.000
PKSG-2b

UniProt

c.934C>G p.His312Asp missense variant moderate contig700 1950704

IGV: Start, Jump

G/C
NGS:
0.410
C90:
0.000
PKSG-2b

UniProt

c.31A>T p.Thr11Ser missense variant moderate contig700 1951851

IGV: Start, Jump

T/A
NGS:
0.844
C90:
0.880
PKSG-4b

UniProt

c.431T>G p.Val144Gly missense variant moderate contig700 2721242

IGV: Start, Jump

A/C
NGS:
0.575
C90:
0.679
aPT1

UniProt

c.406A>G p.Ile136Val missense variant moderate contig121 2839605

IGV: Start, Jump

A/G
NGS:
0.579
C90:
0.761
aPT1

UniProt

c.629C>T p.Thr210Ile missense variant moderate contig121 2840237

IGV: Start, Jump

C/T
NGS:
0.561
C90:
0.598

Nearest genetic relatives (All Samples)

0 0.058 0.117 0.175 0.233
clone distance sibling distance more distant
  1. 0.008 The Gift (RSP10988)
  2. 0.142 Pure Power Plant (RSP11265)
  3. 0.162 Gorilla Cookies (RSP11231)
  4. 0.164 RKM-2018-032 (RSP11124)
  5. 0.164 RKM-2018-017 (RSP11109)
  6. 0.165 RKM-2018-021 (RSP11113)
  7. 0.172 Skywalker OG (RSP10837)
  8. 0.172 SFVxTK (RSP11072)
  9. 0.174 RKM-2018-013 (RSP11104)
  10. 0.177 Rugburn OG (RSP11353)
  11. 0.179 LEMONCAKE (RSP11340)
  12. 0.186 RKM-2018-034 (RSP11126)
  13. 0.194 Kimbo Slice (RSP10997)
  14. 0.195 JABBA S STASH (RSP11348)
  15. 0.196 Red Eye OG (RSP11190)
  16. 0.197 Kush Hemp E1 (RSP11128)
  17. 0.199 Pie Hoe (RSP11073)
  18. 0.202 RKM-2018-012 (RSP11103)
  19. 0.211 SHERBERT (RSP11355)
  20. 0.211 Square Wave (RSP11344)

Nearest genetic relatives (Base Tree)

0 0.075 0.150 0.225 0.300
clone distance sibling distance more distant
  1. 0.007 The Gift (RSP10988)
  2. 0.160 RKM-2018-032 (RSP11124)
  3. 0.168 Skywalker OG (RSP10837)
  4. 0.183 RKM-2018-034 (RSP11126)
  5. 0.191 Kimbo Slice (RSP10997)
  6. 0.193 Kush Hemp E1 (RSP11128)
  7. 0.201 Pie Hoe (RSP11073)
  8. 0.211 RKM-2018-026 (RSP11118)
  9. 0.223 Blueberry Cheesecake (RSP10684)
  10. 0.230 Hermaphrodite ResearchSample2 (RSP11050)
  11. 0.241 RKM-2018-033 (RSP11125)
  12. 0.249 Blueberry Cheesecake (RSP10680)
  13. 0.255 Liberty Haze (RSP11000)
  14. 0.265 Recon (RSP10755)
  15. 0.266 Golden Goat 2 (RSP10991)
  16. 0.269 RKM-2018-020 (RSP11112)
  17. 0.274 Skunk 18 (RSP11038)
  18. 0.277 Hermaphrodite Research Sample1 (RSP11049)
  19. 0.279 Sour Raspberry (RSP10551)
  20. 0.281 RKM-2018-029 (RSP11121)

Most genetically distant strains (All Samples)

0 0.125 0.250 0.375 0.500
clone distance sibling distance more distant
  1. 0.473 Unknown- Cherry Wine - 001 (RSP11268)
  2. 0.463 Cherry Blossom (RSP11328)
  3. 0.463 Cherry Blossom (RSP11323)
  4. 0.462 Cherry Blossom (RSP11318)
  5. 0.447 Unknown- Cherry Wine - 003 (RSP11270)
  6. 0.441 Cherry Blossom (RSP11301)
  7. 0.440 Unknown- Cherry Wine - 002 (RSP11269)
  8. 0.436 Cherry Blossom (RSP11309)
  9. 0.434 Cherry Blossom (RSP11274)
  10. 0.428 Cherry Blossom (RSP11298)
  11. 0.425 Cherry Blossom (RSP11325)
  12. 0.424 Cherry Blossom (RSP11312)
  13. 0.423 Cherry Blossom (RSP11306)
  14. 0.423 Cherry Blossom (RSP11300)
  15. 0.415 Wife (RSP11148)
  16. 0.409 Cherry Blossom (RSP11311)
  17. 0.408 Cherry Blossom (RSP11331)
  18. 0.405 Cherry Blossom (RSP11330)
  19. 0.403 AVIDEKEL USA (RSP11169)
  20. 0.403 Cherry Blossom (RSP11314)

Most genetically distant strains (Base Tree)

0 0.100 0.200 0.300 0.400
clone distance sibling distance more distant
  1. 0.377 JL yellow (RSP11075)
  2. 0.377 Cbot-2019-005 (RSP11133)
  3. 0.376 Cherry (RSP11142)
  4. 0.372 Cbot-2019-004 (RSP11132)
  5. 0.365 Cherry (RSP11143)
  6. 0.362 RKM-2018-006 (RSP11097)
  7. 0.354 Blueberry Cheesecake (RSP10672)
  8. 0.350 Feral (RSP10890)
  9. 0.347 Carmagnola (RSP11037)
  10. 0.344 Monoica (RSP10241)
  11. 0.344 RKM-2018-003 (RSP11094)
  12. 0.344 Carmagnola (RSP10979)
  13. 0.340 UP Sunrise (RSP10989)
  14. 0.339 RKM-2018-027 (RSP11119)
  15. 0.336 Santhica27 (RSP11047)
  16. 0.335 Lovrin (RSP10658)
  17. 0.335 Black Beauty (RSP11035)
  18. 0.333 Italian Kiss (RSP11034)
  19. 0.333 Cbot-2019-001 (RSP11129)
  20. 0.329 RKM-2018-031 (RSP11123)

Nearest genetic relative in Phylos dataset

Phylos Strain SRR4448505
Overlapping SNPs:
108
Concordance:
108

Nearest genetic relative in Lynch dataset

Lynch Strain SRR3495308
Overlapping SNPs:
13
Concordance:
13

Blockchain Registration Information

Transaction ID
b52b1ebc5e2e2ec762a96cd640d4bec3844f8d4363ab829f703de712f9c3d638
Stamping Certificate
Download PDF (853.3 KB)
SHASUM Hash
77c367b7cb3927aa2729d44240e2dbcbb7245cb44455629c74a5fb02117e514c
QR code for RSP11099

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