RKM-2018-009

RSP 11100

Grower: R-Kiem Seeds

General Information

Accession Date
September 6, 2018
Reported Plant Sex
Female
DNA Extracted From
Stem

The strain rarity visualization shows how distant the strain is from the other cultivars in the Kannapedia database. The y-axis represents genetic distance, getting farther as you go up. The width of the visualization at any position along the y-axis shows how many strains there are in the database at that genetic distance. So, a common strain will have a more bottom-heavy shape, while uncommon and rare cultivars will have a visualization that is generally shifted towards the top.

Rarity: Rare
Most Distant Most Similar

Chemical Information

Cannabinoid and terpenoid information provided by the grower.

Cannabinoids

No information provided.

Terpenoids

No information provided.

Genetic Information

Plant Type
Type I

The bell curve in the heterozygosity visualization shows the distribution of heterozygosity levels for cannabis cultivars in the Kannapedia database. The green line shows where this particular strain fits within the distribution. Heterozygosity is associated with heterosis (aka hybrid vigor) but also leads to the production of more variable offspring. When plants have two genetically different parents, heterozygosity levels will be higher than if it has been inbred or backcrossed repeatedly.

Heterozygosity: 0.84%
Least Heterozygous Most Heterozygous

The ratio of reads mapped to Y-contigs to reads mapped to the whole Cannabis genome (Y-ratios) has been demonstrated to be strongly correlated with plant sex typing. This plot shows the distribution of Y-ratios for all samples in our database which were sequenced with the same method (panel or WGS) as this sample and where this sample falls in the distribution.

Y-Ratio Distribution: 0.0386
male female RSP11100

This chart represents the Illumina sequence coverage over the Bt/Bd allele. These are the three regions in the cannabis genome that impact THCA, CBDA, CBGA production. Coverage over the Active CBDAS gene is highly correlated with Type II and Type III plants as described by Etienne de Meijer. Coverage over the THCA gene is highly correlated with Type I and Type II plants but is anti-correlated with Type III plants. Type I plants require coverage over the inactive CBDA loci and no coverage over the Active CBDA gene. Lack of coverage over the Active CBDA and Active THCA allele are presumed to be Type IV plants (CBGA dominant). While deletions of entire THCAS and CBDAS genes are the most common Bt:Bd alleles observed, it is possible to have plants with these genes where functional expression of the enzyme is disrupted by deactivating point mutations (Kojoma et al. 2006).

Bt/Bd Allele Coverage

This chart represents the Illumina sequence coverage over the CBCA synthase gene.

CBCAS Coverage

Variants (THCAS, CBDAS, and CBCAS)

Gene HGVS.c HGVS.p Annotation Annotation Impact Contig Contig Pos Ref/Alt Var Freq
THCAS c.998C>G p.Pro333Arg missense variant moderate contig741 4416830

IGV: Start, Jump

G/C
NGS:
0.182
C90:
0.000

Variants (Select Genes of Interest)

PKSG-2a

UniProt

c.67T>A p.Phe23Ile missense variant moderate contig700 1945567

IGV: Start, Jump

A/T
NGS:
0.825
C90:
0.904
PKSG-2b

UniProt

c.1152T>A p.Asn384Lys missense variant moderate contig700 1950486

IGV: Start, Jump

A/T
NGS:
0.715
C90:
0.895
PKSG-2b

UniProt

c.1132C>G p.Leu378Val missense variant moderate contig700 1950506

IGV: Start, Jump

G/C
NGS:
0.717
C90:
0.000
PKSG-2b

UniProt

c.1117A>G p.Ile373Val missense variant moderate contig700 1950521

IGV: Start, Jump

T/C
NGS:
0.807
C90:
0.981
PKSG-2b

UniProt

c.948T>G p.Asp316Glu missense variant moderate contig700 1950690

IGV: Start, Jump

A/C
NGS:
0.456
C90:
0.000
PKSG-2b

UniProt

c.945T>G p.Ser315Arg missense variant moderate contig700 1950693

IGV: Start, Jump

A/C
NGS:
0.454
C90:
0.000
PKSG-2b

UniProt

c.944G>A p.Ser315Asn missense variant moderate contig700 1950694

IGV: Start, Jump

C/T
NGS:
0.445
C90:
0.000
PKSG-2b

UniProt

c.934C>G p.His312Asp missense variant moderate contig700 1950704

IGV: Start, Jump

G/C
NGS:
0.410
C90:
0.000
PKSG-2b

UniProt

c.31A>T p.Thr11Ser missense variant moderate contig700 1951851

IGV: Start, Jump

T/A
NGS:
0.844
C90:
0.880
PKSG-2b

UniProt

c.-2_1dupATA start lost & conservative inframe insertion high contig700 1951880

IGV: Start, Jump

A/ATAT
NGS:
0.410
C90:
0.000
PKSG-4b

UniProt

c.527G>T p.Trp176Leu missense variant moderate contig700 2721146

IGV: Start, Jump

C/A
NGS:
0.033
C90:
0.000
PKSG-4b

UniProt

c.526T>C p.Trp176Arg missense variant moderate contig700 2721147

IGV: Start, Jump

A/G
NGS:
0.037
C90:
0.000
PKSG-4b

UniProt

c.496A>G p.Lys166Glu missense variant moderate contig700 2721177

IGV: Start, Jump

T/C
NGS:
0.651
C90:
0.684
PKSG-4b

UniProt

c.489delT p.Phe163fs frameshift variant high contig700 2721183

IGV: Start, Jump

CA/C
NGS:
0.792
C90:
0.761
PKSG-4b

UniProt

c.485A>G p.Lys162Arg missense variant moderate contig700 2721188

IGV: Start, Jump

T/C
NGS:
0.643
C90:
0.301
PKSG-4b

UniProt

c.431T>G p.Val144Gly missense variant moderate contig700 2721242

IGV: Start, Jump

A/C
NGS:
0.575
C90:
0.679
PKSG-4b

UniProt

c.419A>G p.Asp140Gly missense variant moderate contig700 2721254

IGV: Start, Jump

T/C
NGS:
0.430
C90:
0.440
aPT4

UniProt

c.302A>G p.Asn101Ser missense variant moderate contig121 2829099

IGV: Start, Jump

A/G
NGS:
0.349
C90:
0.000
aPT4

UniProt

c.1168T>C p.Tyr390His missense variant moderate contig121 2833503

IGV: Start, Jump

T/C
NGS:
0.546
C90:
0.000
aPT1

UniProt

c.629C>T p.Thr210Ile missense variant moderate contig121 2840237

IGV: Start, Jump

C/T
NGS:
0.561
C90:
0.598

Nearest genetic relatives (All Samples)

0 0.058 0.117 0.175 0.233
clone distance sibling distance more distant
  1. 0.182 JL Cross 13 (RSP11514)
  2. 0.191 OR 05MAY2017 (RSP10940)
  3. 0.208 RKM-2018-010 (RSP11101)
  4. 0.209 Blue Dream (RSP11007)
  5. 0.210 RKM-2018-011 (RSP11102)
  6. 0.210 Serious Happiness (RSP10763)
  7. 0.212 Dominion Skunk (RSP11354)
  8. 0.215 Blue Dream (RSP11006)
  9. 0.215 Mother s Milk No 31 (RSP11623)
  10. 0.216 JL Cross 3 (RSP11504)
  11. 0.219 Power Plant (RSP11223)
  12. 0.220 Blue Dream (RSP11009)
  13. 0.221 Headcheese (RSP11192)
  14. 0.222 Domnesia (RSP11184)
  15. 0.222 Blue Dream (RSP11017)
  16. 0.223 Super Blue Dream (RSP11011)
  17. 0.225 Blue Dream (RSP11010)
  18. 0.226 Lime OG (RSP12101)
  19. 0.226 JL Cross 1 (RSP11502)
  20. 0.226 Blue Dream (RSP11342)

Most genetically distant strains (All Samples)

0 0.133 0.267 0.400 0.533
clone distance sibling distance more distant
  1. 0.506 Cherry Blossom (RSP11311)
  2. 0.462 Cherry Blossom (RSP11328)
  3. 0.450 Cherry Blossom (RSP11314)
  4. 0.449 Cherry Blossom (RSP11335)
  5. 0.449 Cherry Blossom (RSP11308)
  6. 0.447 Cherry Blossom (RSP11312)
  7. 0.438 Cherry Blossom (RSP11317)
  8. 0.435 Cherry Blossom (RSP11298)
  9. 0.434 Cherry Blossom (RSP11309)
  10. 0.427 Cherry Blossom (RSP11334)
  11. 0.426 Unknown--Cherry Wine---003- (RSP11270)
  12. 0.423 Cherry Blossom (RSP11323)
  13. 0.423 Avidekel 05MAY2017 (RSP10938)
  14. 0.418 Cherry Blossom (RSP11300)
  15. 0.414 Unknown--Cherry Wine---001- (RSP11268)
  16. 0.411 Cherry Blossom (RSP11324)
  17. 0.410 Unknown--Cherry Wine---002- (RSP11269)
  18. 0.409 Cherry Blossom (RSP11333)
  19. 0.409 Cherry Blossom (RSP11330)
  20. 0.407 Cherry Blossom (RSP11332)

Nearest genetic relative in Phylos dataset

Phylos Strain SRR8349039
Overlapping SNPs:
118
Concordance:
84

Nearest genetic relative in Lynch dataset

Lynch Strain SRR3495250
Overlapping SNPs:
3
Concordance:
3

Blockchain Registration Information

Transaction ID
60f0ab929a77d14e936a3f598723943772986f589f76aea5a63aaefff31bb4e2
Stamping Certificate
Download PDF (867.9 KB)
SHASUM Hash
aeebc9b1623262de492fe8d8fbd9b46f9afdf3b7c50cb661e93d6b086799b806
QR code for RSP11100

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