RKM-2018-010

RSP 11101

Grower: R-Kiem Seeds

General Information

Accession Date
September 6, 2018
Reported Plant Sex
Female

The strain rarity visualization shows how distant the strain is from the other cultivars in the Kannapedia database. The y-axis represents genetic distance, getting farther as you go up. The width of the visualization at any position along the y-axis shows how many strains there are in the database at that genetic distance. So, a common strain will have a more bottom-heavy shape, while uncommon and rare cultivars will have a visualization that is generally shifted towards the top.

Rarity: Rare
Most Distant Most Similar

Chemical Information

Cannabinoid and terpenoid information provided by the grower.

Cannabinoids

No information provided.

Terpenoids

No information provided.

Genetic Information

Plant Type
Type I

The bell curve in the heterozygosity visualization shows the distribution of heterozygosity levels for cannabis cultivars in the Kannapedia database. The green line shows where this particular strain fits within the distribution. Heterozygosity is associated with heterosis (aka hybrid vigor) but also leads to the production of more variable offspring. When plants have two genetically different parents, heterozygosity levels will be higher than if it has been inbred or backcrossed repeatedly.

Heterozygosity: 0.76%
Least Heterozygous Most Heterozygous

The ratio of reads mapped to Y-contigs to reads mapped to the whole Cannabis genome (Y-ratios) has been demonstrated to be strongly correlated with plant sex typing. This plot shows the distribution of Y-ratios for all samples in our database which were sequenced with the same method (panel or WGS) as this sample and where this sample falls in the distribution.

Y-Ratio Distribution: 0.0393
male female RSP11101

This chart represents the Illumina sequence coverage over the Bt/Bd allele. These are the three regions in the cannabis genome that impact THCA, CBDA, CBGA production. Coverage over the Active CBDAS gene is highly correlated with Type II and Type III plants as described by Etienne de Meijer. Coverage over the THCA gene is highly correlated with Type I and Type II plants but is anti-correlated with Type III plants. Type I plants require coverage over the inactive CBDA loci and no coverage over the Active CBDA gene. Lack of coverage over the Active CBDA and Active THCA allele are presumed to be Type IV plants (CBGA dominant). While deletions of entire THCAS and CBDAS genes are the most common Bt:Bd alleles observed, it is possible to have plants with these genes where functional expression of the enzyme is disrupted by deactivating point mutations (Kojoma et al. 2006).

Bt/Bd Allele Coverage

This chart represents the Illumina sequence coverage over the CBCA synthase gene.

CBCAS Coverage

Variants (THCAS, CBDAS, and CBCAS)

Gene HGVS.c HGVS.p Annotation Annotation Impact Contig Contig Pos Ref/Alt Var Freq
THCAS c.998C>G p.Pro333Arg missense variant moderate contig741 4416830

IGV: Start, Jump

G/C
NGS:
0.182
C90:
0.000

Variants (Select Genes of Interest)

PKSG-2a

UniProt

c.67T>A p.Phe23Ile missense variant moderate contig700 1945567

IGV: Start, Jump

A/T
NGS:
0.825
C90:
0.904
PKSG-2b

UniProt

c.1152T>A p.Asn384Lys missense variant moderate contig700 1950486

IGV: Start, Jump

A/T
NGS:
0.715
C90:
0.895
PKSG-2b

UniProt

c.1132C>G p.Leu378Val missense variant moderate contig700 1950506

IGV: Start, Jump

G/C
NGS:
0.717
C90:
0.000
PKSG-2b

UniProt

c.1117A>G p.Ile373Val missense variant moderate contig700 1950521

IGV: Start, Jump

T/C
NGS:
0.807
C90:
0.981
PKSG-2b

UniProt

c.948T>G p.Asp316Glu missense variant moderate contig700 1950690

IGV: Start, Jump

A/C
NGS:
0.456
C90:
0.000
PKSG-2b

UniProt

c.944G>A p.Ser315Asn missense variant moderate contig700 1950694

IGV: Start, Jump

C/T
NGS:
0.445
C90:
0.000
PKSG-2b

UniProt

c.31A>T p.Thr11Ser missense variant moderate contig700 1951851

IGV: Start, Jump

T/A
NGS:
0.844
C90:
0.880
PKSG-4b

UniProt

c.496A>G p.Lys166Glu missense variant moderate contig700 2721177

IGV: Start, Jump

T/C
NGS:
0.651
C90:
0.684
PKSG-4b

UniProt

c.489delT p.Phe163fs frameshift variant high contig700 2721183

IGV: Start, Jump

CA/C
NGS:
0.792
C90:
0.761
PKSG-4b

UniProt

c.485A>G p.Lys162Arg missense variant moderate contig700 2721188

IGV: Start, Jump

T/C
NGS:
0.643
C90:
0.301
PKSG-4b

UniProt

c.419A>G p.Asp140Gly missense variant moderate contig700 2721254

IGV: Start, Jump

T/C
NGS:
0.430
C90:
0.440
aPT4

UniProt

c.1168T>C p.Tyr390His missense variant moderate contig121 2833503

IGV: Start, Jump

T/C
NGS:
0.546
C90:
0.000
aPT1

UniProt

c.406A>G p.Ile136Val missense variant moderate contig121 2839605

IGV: Start, Jump

A/G
NGS:
0.579
C90:
0.761
aPT1

UniProt

c.629C>T p.Thr210Ile missense variant moderate contig121 2840237

IGV: Start, Jump

C/T
NGS:
0.561
C90:
0.598

Nearest genetic relatives (All Samples)

0 0.067 0.133 0.200 0.267
clone distance sibling distance more distant
  1. 0.195 RKM-2018-011 (RSP11102)
  2. 0.208 RKM-2018-009 (RSP11100)
  3. 0.209 RKM-2018-033 (RSP11125)
  4. 0.216 T S A G E (RSP11351)
  5. 0.216 OR 05MAY2017 (RSP10940)
  6. 0.220 Blue Dream (RSP11007)
  7. 0.221 Blue Dream (RSP11017)
  8. 0.221 Doug s Varin (RSP11243)
  9. 0.227 JL Cross 13 (RSP11514)
  10. 0.227 BLACK JACK (RSP11346)
  11. 0.230 Black Jack (RSP10603)
  12. 0.230 Blue Dream (RSP11342)
  13. 0.231 Sour Raspberry (RSP10551)
  14. 0.233 Blue Dream (RSP11006)
  15. 0.234 Durban Poison #1 (RSP11013)
  16. 0.236 Hermaphrodite ResearchSample2 (RSP11050)
  17. 0.236 Colombian 8 (SRR14708214)
  18. 0.236 Rest (RSP11377)
  19. 0.238 Hindu Kush (SRR14708261)
  20. 0.239 RKM-2018-016 (RSP11108)

Nearest genetic relatives (Base Tree)

0 0.075 0.150 0.225 0.300
clone distance sibling distance more distant
  1. 0.207 RKM-2018-033 (RSP11125)
  2. 0.215 RKM-2018-009 (RSP11100)
  3. 0.223 Sour Raspberry (RSP10551)
  4. 0.230 Liberty Haze (RSP11000)
  5. 0.236 UP Sunrise (RSP10989)
  6. 0.240 Gold Cracker (RSP11048)
  7. 0.245 Italian Kiss (RSP11034)
  8. 0.245 Blue Dream (RSP11033)
  9. 0.247 RKM-2018-028 (RSP11120)
  10. 0.249 Hermaphrodite ResearchSample2 (RSP11050)
  11. 0.253 Skunk#18 (RSP11038)
  12. 0.254 CST (RSP11002)
  13. 0.256 RKM-2018-027 (RSP11119)
  14. 0.259 Durban Poison (RSP11014)
  15. 0.259 RKM-2018-018 (RSP11110)
  16. 0.263 RKM-2018-020 (RSP11112)
  17. 0.268 RKM-2018-006 (RSP11097)
  18. 0.271 Hermaphrodite Research Sample1 (RSP11049)
  19. 0.274 Recon (RSP10755)
  20. 0.280 Blueberry Cheesecake (RSP10684)

Most genetically distant strains (All Samples)

0 0.117 0.233 0.350 0.467
clone distance sibling distance more distant
  1. 0.460 Cherry Blossom (RSP11311)
  2. 0.451 Cherry Blossom (RSP11317)
  3. 0.449 Cherry Blossom (RSP11328)
  4. 0.448 Cherry Blossom (RSP11335)
  5. 0.446 Cherry Blossom (RSP11308)
  6. 0.445 Cherry Blossom (RSP11312)
  7. 0.441 Cherry Blossom (RSP11309)
  8. 0.429 Cherry Blossom (RSP11334)
  9. 0.428 Cherry Blossom (RSP11314)
  10. 0.426 Cherry Blossom (RSP11324)
  11. 0.425 80E (RSP11213)
  12. 0.423 Cherry Blossom (RSP11298)
  13. 0.422 Cherry Blossom (RSP11330)
  14. 0.421 CS (RSP11208)
  15. 0.420 Cherry Blossom (RSP11333)
  16. 0.418 Cherry Blossom (RSP11300)
  17. 0.412 BagSeed (RSP12627)
  18. 0.409 Cherry Blossom (RSP11310)
  19. 0.409 Unknown--Cherry Wine---003- (RSP11270)
  20. 0.408 Cherry Blossom (RSP11318)

Most genetically distant strains (Base Tree)

0 0.100 0.200 0.300 0.400
clone distance sibling distance more distant
  1. 0.370 Cbot-2019-005 (RSP11133)
  2. 0.366 Carmagnola (RSP11037)
  3. 0.365 Feral (RSP10890)
  4. 0.359 RKM-2018-002 (RSP11093)
  5. 0.359 Cherry (RSP11142)
  6. 0.353 Santhica27 (RSP11047)
  7. 0.345 Monoica (RSP10241)
  8. 0.342 RKM-2018-026 (RSP11118)
  9. 0.342 RKM-2018-034 (RSP11126)
  10. 0.340 Blueberry Cheesecake (RSP10672)
  11. 0.339 Skywalker OG (RSP10837)
  12. 0.335 Futura 75 (RSP10664)
  13. 0.334 Cherry (RSP11143)
  14. 0.334 Tisza (RSP11044)
  15. 0.334 Carmagnola (RSP10979)
  16. 0.333 Kush Hemp E1 (RSP11128)
  17. 0.332 KYRG-11 (RSP11051)
  18. 0.329 Ivory (RSP10668)
  19. 0.325 Kyrgyz Gold (RSP11054)
  20. 0.321 Tisza (RSP10659)

Nearest genetic relative in Phylos dataset

Phylos Strain SRR4448447
Overlapping SNPs:
100
Concordance:
65

Nearest genetic relative in Lynch dataset

Lynch Strain SRR3495314
Overlapping SNPs:
2
Concordance:
2

Blockchain Registration Information

Transaction ID
16482fc6ff9bf203788bd4d0815719182a0773447fbbcf8b3e3b9ec1a20714a3
Stamping Certificate
Download PDF (845.6 KB)
SHASUM Hash
feb0b99e9f506cd56fc16b0045854b4e5064ff96549b1c50a24f37d1832d5c39
QR code for RSP11101

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