RKM-2018-013

RSP 11104

Grower: R-Kiem Seeds

General Information

Accession Date
September 6, 2018
Reported Plant Sex
Female

The strain rarity visualization shows how distant the strain is from the other cultivars in the Kannapedia database. The y-axis represents genetic distance, getting farther as you go up. The width of the visualization at any position along the y-axis shows how many strains there are in the database at that genetic distance. So, a common strain will have a more bottom-heavy shape, while uncommon and rare cultivars will have a visualization that is generally shifted towards the top.

Rarity: Uncommon
Most Distant Most Similar

Chemical Information

Cannabinoid and terpenoid information provided by the grower.

Cannabinoids

No information provided.

Terpenoids

No information provided.

Genetic Information

Plant Type
Type I

The bell curve in the heterozygosity visualization shows the distribution of heterozygosity levels for cannabis cultivars in the Kannapedia database. The green line shows where this particular strain fits within the distribution. Heterozygosity is associated with heterosis (aka hybrid vigor) but also leads to the production of more variable offspring. When plants have two genetically different parents, heterozygosity levels will be higher than if it has been inbred or backcrossed repeatedly.

Heterozygosity: 0.9%
Least Heterozygous Most Heterozygous

The ratio of reads mapped to Y-contigs to reads mapped to the whole Cannabis genome (Y-ratios) has been demonstrated to be strongly correlated with plant sex typing. This plot shows the distribution of Y-ratios for all samples in our database which were sequenced with the same method (panel or WGS) as this sample and where this sample falls in the distribution.

Y-Ratio Distribution: 0.0450
male female RSP11104

This chart represents the Illumina sequence coverage over the Bt/Bd allele. These are the three regions in the cannabis genome that impact THCA, CBDA, CBGA production. Coverage over the Active CBDAS gene is highly correlated with Type II and Type III plants as described by Etienne de Meijer. Coverage over the THCA gene is highly correlated with Type I and Type II plants but is anti-correlated with Type III plants. Type I plants require coverage over the inactive CBDA loci and no coverage over the Active CBDA gene. Lack of coverage over the Active CBDA and Active THCA allele are presumed to be Type IV plants (CBGA dominant). While deletions of entire THCAS and CBDAS genes are the most common Bt:Bd alleles observed, it is possible to have plants with these genes where functional expression of the enzyme is disrupted by deactivating point mutations (Kojoma et al. 2006).

Bt/Bd Allele Coverage

This chart represents the Illumina sequence coverage over the CBCA synthase gene.

CBCAS Coverage

Variants (THCAS, CBDAS, and CBCAS)

Gene HGVS.c HGVS.p Annotation Annotation Impact Contig Contig Pos Ref/Alt Var Freq
THCAS c.998C>G p.Pro333Arg missense variant moderate contig741 4416830

IGV: Start, Jump

G/C
NGS:
0.182
C90:
0.000

Variants (Select Genes of Interest)

PKSG-2a

UniProt

c.67T>A p.Phe23Ile missense variant moderate contig700 1945567

IGV: Start, Jump

A/T
NGS:
0.825
C90:
0.904
PKSG-2a

UniProt

c.31A>T p.Thr11Ser missense variant moderate contig700 1945603

IGV: Start, Jump

T/A
NGS:
0.763
C90:
0.876
PKSG-2b

UniProt

c.1152T>A p.Asn384Lys missense variant moderate contig700 1950486

IGV: Start, Jump

A/T
NGS:
0.715
C90:
0.895
PKSG-2b

UniProt

c.1132C>G p.Leu378Val missense variant moderate contig700 1950506

IGV: Start, Jump

G/C
NGS:
0.717
C90:
0.000
PKSG-2b

UniProt

c.1117A>G p.Ile373Val missense variant moderate contig700 1950521

IGV: Start, Jump

T/C
NGS:
0.807
C90:
0.981
PKSG-2b

UniProt

c.31A>T p.Thr11Ser missense variant moderate contig700 1951851

IGV: Start, Jump

T/A
NGS:
0.844
C90:
0.880
PKSG-2b

UniProt

c.-2_1dupATA start lost & conservative inframe insertion high contig700 1951880

IGV: Start, Jump

A/ATAT
NGS:
0.410
C90:
0.000
aPT1

UniProt

c.406A>G p.Ile136Val missense variant moderate contig121 2839605

IGV: Start, Jump

A/G
NGS:
0.579
C90:
0.761
aPT1

UniProt

c.629C>T p.Thr210Ile missense variant moderate contig121 2840237

IGV: Start, Jump

C/T
NGS:
0.561
C90:
0.598

Nearest genetic relatives (All Samples)

0 0.050 0.100 0.150 0.200
clone distance sibling distance more distant
  1. 0.059 SFVxTK (RSP11072)
  2. 0.066 Skywalker OG (RSP10837)
  3. 0.070 Red Eye OG (RSP11190)
  4. 0.110 Rugburn OG (RSP11353)
  5. 0.152 Pie Hoe (RSP11073)
  6. 0.153 RKM-2018-012 (RSP11103)
  7. 0.154 Pure Power Plant (RSP11265)
  8. 0.155 Casco Kush (RSP11167)
  9. 0.162 501st OG (RSP11241)
  10. 0.164 RKM-2018-026 (RSP11118)
  11. 0.171 The Gift (RSP10988)
  12. 0.173 Gorilla Cookies (RSP11231)
  13. 0.174 RKM-2018-008 (RSP11099)
  14. 0.178 Garlic (RSP11341)
  15. 0.179 RKM-2018-034 (RSP11126)
  16. 0.193 Star Dawg (RSP11352)
  17. 0.195 RKM-2018-017 (RSP11109)
  18. 0.196 RKM-2018-021 (RSP11113)
  19. 0.196 RKM-2018-032 (RSP11124)
  20. 0.196 Sunday Driver (RSP11071)

Nearest genetic relatives (Base Tree)

0 0.075 0.150 0.225 0.300
clone distance sibling distance more distant
  1. 0.067 Skywalker OG (RSP10837)
  2. 0.154 Pie Hoe (RSP11073)
  3. 0.165 RKM-2018-026 (RSP11118)
  4. 0.165 The Gift (RSP10988)
  5. 0.178 RKM-2018-034 (RSP11126)
  6. 0.198 RKM-2018-032 (RSP11124)
  7. 0.213 Hermaphrodite ResearchSample2 (RSP11050)
  8. 0.218 RKM-2018-002 (RSP11093)
  9. 0.223 Blueberry Cheesecake (RSP10684)
  10. 0.232 RKM-2018-033 (RSP11125)
  11. 0.233 Kimbo Slice (RSP10997)
  12. 0.234 Hermaphrodite Research Sample1 (RSP11049)
  13. 0.239 RKM-2018-020 (RSP11112)
  14. 0.241 Liberty Haze (RSP11000)
  15. 0.242 RKM-2018-005 (RSP11096)
  16. 0.251 Kush Hemp E1 (RSP11128)
  17. 0.260 Gold Cracker (RSP11048)
  18. 0.272 Blueberry Cheesecake (RSP10680)
  19. 0.273 Recon (RSP10755)
  20. 0.277 RKM-2018-029 (RSP11121)

Most genetically distant strains (All Samples)

0 0.133 0.267 0.400 0.533
clone distance sibling distance more distant
  1. 0.508 Unknown- Cherry Wine - 001 (RSP11268)
  2. 0.499 Cherry Blossom (RSP11318)
  3. 0.486 Cherry Blossom (RSP11323)
  4. 0.480 Cherry Blossom (RSP11301)
  5. 0.469 Unknown- Cherry Wine - 002 (RSP11269)
  6. 0.467 Unknown- Cherry Wine - 003 (RSP11270)
  7. 0.461 Cherry Blossom (RSP11328)
  8. 0.448 Wife (RSP11148)
  9. 0.442 Cherry Blossom (RSP11315)
  10. 0.441 Cherry Blossom (RSP11325)
  11. 0.440 Cherry Blossom (RSP11312)
  12. 0.439 Cherry Blossom (RSP11334)
  13. 0.439 Cherry Blossom (RSP11300)
  14. 0.438 Cherry Blossom (RSP11331)
  15. 0.438 Cherry Blossom (RSP11274)
  16. 0.435 Cherry Blossom (RSP11298)
  17. 0.434 Cherry Blossom (RSP11309)
  18. 0.434 Cherry Blossom (RSP11311)
  19. 0.429 Cherry Blossom (RSP11310)
  20. 0.428 Cherry Blossom (RSP11319)

Most genetically distant strains (Base Tree)

0 0.100 0.200 0.300 0.400
clone distance sibling distance more distant
  1. 0.385 Cbot-2019-005 (RSP11133)
  2. 0.385 JL yellow (RSP11075)
  3. 0.379 Cherry (RSP11143)
  4. 0.369 Cherry (RSP11142)
  5. 0.358 Blueberry Cheesecake (RSP10672)
  6. 0.356 RKM-2018-006 (RSP11097)
  7. 0.356 Cbot-2019-004 (RSP11132)
  8. 0.347 Black Beauty (RSP11035)
  9. 0.344 Feral (RSP10890)
  10. 0.343 Cbot-2019-001 (RSP11129)
  11. 0.341 Carmagnola (RSP11037)
  12. 0.338 Carmagnola (RSP10979)
  13. 0.337 Monoica (RSP10241)
  14. 0.335 Futura 75 (RSP10664)
  15. 0.329 RKM-2018-003 (RSP11094)
  16. 0.327 UP Sunrise (RSP10989)
  17. 0.327 RKM-2018-023 (RSP11115)
  18. 0.326 Ivory (RSP10668)
  19. 0.324 Lovrin (RSP10658)
  20. 0.322 Fedora 17 (RSP10661)

Nearest genetic relative in Phylos dataset

Phylos Strain SRR8347029
Overlapping SNPs:
118
Concordance:
101

Nearest genetic relative in Lynch dataset

Lynch Strain SRR3495250
Overlapping SNPs:
3
Concordance:
3

Blockchain Registration Information

Transaction ID
263b6936ba2801937abfa74c8101e941f8b09c2a01c19f1702d3b86b1e1ae083
Stamping Certificate
Download PDF (860.5 KB)
SHASUM Hash
9c5522d477748b0c0a0a351a8650938311eb625b8a4ff47799382ebb0a5c5af2
QR code for RSP11104

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