RKM-2018-027

RSP 11119

Grower: R-Kiem Seeds

General Information

Accession Date
October 21, 2018
Reported Plant Sex
Female

The strain rarity visualization shows how distant the strain is from the other cultivars in the Kannapedia database. The y-axis represents genetic distance, getting farther as you go up. The width of the visualization at any position along the y-axis shows how many strains there are in the database at that genetic distance. So, a common strain will have a more bottom-heavy shape, while uncommon and rare cultivars will have a visualization that is generally shifted towards the top.

Rarity: Uncommon
Most Distant Most Similar

Chemical Information

Cannabinoid and terpenoid information provided by the grower.

Cannabinoids

No information provided.

Terpenoids

No information provided.

Genetic Information

Plant Type
Type I

The bell curve in the heterozygosity visualization shows the distribution of heterozygosity levels for cannabis cultivars in the Kannapedia database. The green line shows where this particular strain fits within the distribution. Heterozygosity is associated with heterosis (aka hybrid vigor) but also leads to the production of more variable offspring. When plants have two genetically different parents, heterozygosity levels will be higher than if it has been inbred or backcrossed repeatedly.

Heterozygosity: 1.33%
Least Heterozygous Most Heterozygous

The ratio of reads mapped to Y-contigs to reads mapped to the whole Cannabis genome (Y-ratios) has been demonstrated to be strongly correlated with plant sex typing. This plot shows the distribution of Y-ratios for all samples in our database which were sequenced with the same method (panel or WGS) as this sample and where this sample falls in the distribution.

Y-Ratio Distribution: 0.0426
male female RSP11119

This chart represents the Illumina sequence coverage over the Bt/Bd allele. These are the three regions in the cannabis genome that impact THCA, CBDA, CBGA production. Coverage over the Active CBDAS gene is highly correlated with Type II and Type III plants as described by Etienne de Meijer. Coverage over the THCA gene is highly correlated with Type I and Type II plants but is anti-correlated with Type III plants. Type I plants require coverage over the inactive CBDA loci and no coverage over the Active CBDA gene. Lack of coverage over the Active CBDA and Active THCA allele are presumed to be Type IV plants (CBGA dominant). While deletions of entire THCAS and CBDAS genes are the most common Bt:Bd alleles observed, it is possible to have plants with these genes where functional expression of the enzyme is disrupted by deactivating point mutations (Kojoma et al. 2006).

Bt/Bd Allele Coverage

This chart represents the Illumina sequence coverage over the CBCA synthase gene.

CBCAS Coverage

Variants (THCAS, CBDAS, and CBCAS)

Gene HGVS.c HGVS.p Annotation Annotation Impact Contig Contig Pos Ref/Alt Var Freq
THCAS c.749C>A p.Ala250Asp missense variant moderate contig741 4417079

IGV: Start, Jump

G/T
NGS:
0.127
C90:
0.632

Variants (Select Genes of Interest)

PKSG-2a

UniProt

c.67T>A p.Phe23Ile missense variant moderate contig700 1945567

IGV: Start, Jump

A/T
NGS:
0.825
C90:
0.904
PKSG-2a

UniProt

c.31A>T p.Thr11Ser missense variant moderate contig700 1945603

IGV: Start, Jump

T/A
NGS:
0.763
C90:
0.876
PKSG-2b

UniProt

c.1152T>A p.Asn384Lys missense variant moderate contig700 1950486

IGV: Start, Jump

A/T
NGS:
0.715
C90:
0.895
PKSG-2b

UniProt

c.1132C>G p.Leu378Val missense variant moderate contig700 1950506

IGV: Start, Jump

G/C
NGS:
0.717
C90:
0.000
PKSG-2b

UniProt

c.1117A>G p.Ile373Val missense variant moderate contig700 1950521

IGV: Start, Jump

T/C
NGS:
0.807
C90:
0.981
PKSG-2b

UniProt

c.948T>G p.Asp316Glu missense variant moderate contig700 1950690

IGV: Start, Jump

A/C
NGS:
0.456
C90:
0.000
PKSG-2b

UniProt

c.945T>G p.Ser315Arg missense variant moderate contig700 1950693

IGV: Start, Jump

A/C
NGS:
0.454
C90:
0.000
PKSG-2b

UniProt

c.944G>A p.Ser315Asn missense variant moderate contig700 1950694

IGV: Start, Jump

C/T
NGS:
0.445
C90:
0.000
PKSG-2b

UniProt

c.934C>G p.His312Asp missense variant moderate contig700 1950704

IGV: Start, Jump

G/C
NGS:
0.410
C90:
0.000
PKSG-2b

UniProt

c.31A>T p.Thr11Ser missense variant moderate contig700 1951851

IGV: Start, Jump

T/A
NGS:
0.844
C90:
0.880
PKSG-2b

UniProt

c.-2_1dupATA start lost & conservative inframe insertion high contig700 1951880

IGV: Start, Jump

A/ATAT
NGS:
0.410
C90:
0.000
PKSG-4b

UniProt

c.496A>G p.Lys166Glu missense variant moderate contig700 2721177

IGV: Start, Jump

T/C
NGS:
0.651
C90:
0.684
PKSG-4b

UniProt

c.489delT p.Phe163fs frameshift variant high contig700 2721183

IGV: Start, Jump

CA/C
NGS:
0.792
C90:
0.761
PKSG-4b

UniProt

c.485A>G p.Lys162Arg missense variant moderate contig700 2721188

IGV: Start, Jump

T/C
NGS:
0.643
C90:
0.301
PKSG-4b

UniProt

c.431T>G p.Val144Gly missense variant moderate contig700 2721242

IGV: Start, Jump

A/C
NGS:
0.575
C90:
0.679
PKSG-4b

UniProt

c.419A>G p.Asp140Gly missense variant moderate contig700 2721254

IGV: Start, Jump

T/C
NGS:
0.430
C90:
0.440
PKSG-4b

UniProt

c.352_355delACAG p.Thr118fs frameshift variant high contig700 2721317

IGV: Start, Jump

CCTGT/C
NGS:
0.529
C90:
0.000
aPT4

UniProt

c.775delT p.Tyr259fs frameshift variant high contig121 2831380

IGV: Start, Jump

AT/A
NGS:
0.224
C90:
0.000
aPT4

UniProt

c.1168T>C p.Tyr390His missense variant moderate contig121 2833503

IGV: Start, Jump

T/C
NGS:
0.546
C90:
0.000
aPT1

UniProt

c.406A>G p.Ile136Val missense variant moderate contig121 2839605

IGV: Start, Jump

A/G
NGS:
0.579
C90:
0.761
aPT1

UniProt

c.629C>T p.Thr210Ile missense variant moderate contig121 2840237

IGV: Start, Jump

C/T
NGS:
0.561
C90:
0.598
HDS-2

UniProt

c.82_93delGTAACCGGAACT p.Val28_Thr31del conservative inframe deletion moderate contig95 1989748

IGV: Start, Jump

CGTAACCGGAACT/C
NGS:
0.805
C90:
0.000
HDS-2

UniProt

c.127T>G p.Ser43Ala missense variant moderate contig95 1989794

IGV: Start, Jump

T/G
NGS:
0.721
C90:
0.000

Nearest genetic relatives (All Samples)

0 0.058 0.117 0.175 0.233
clone distance sibling distance more distant
  1. 0.026 BLACK JACK (RSP11346)
  2. 0.031 RKM-2018-025 (RSP11117)
  3. 0.031 RKM-2018-016 (RSP11108)
  4. 0.039 Black Jack (RSP10603)
  5. 0.168 Gold Cracker (RSP11041)
  6. 0.177 Durban Poison 1 (RSP10996)
  7. 0.177 Gold Cracker (RSP11048)
  8. 0.179 Saint Jack (RSP11179)
  9. 0.182 Durban Poison 1 (RSP11013)
  10. 0.183 Durban Poison (RSP10998)
  11. 0.185 RKM-2018-006 (RSP11097)
  12. 0.186 Cheese (RSP10460)
  13. 0.186 Durban Poison (RSP11014)
  14. 0.192 White Label 1 (RSP11336)
  15. 0.197 RKM-2018-011 (RSP11102)
  16. 0.201 Blue Dream (RSP11007)
  17. 0.202 Tangerine Haze (RSP10995)
  18. 0.207 Super Blue Dream (RSP11011)
  19. 0.211 Durban Poison (RSP11226)
  20. 0.212 OR (RSP10940)

Nearest genetic relatives (Base Tree)

0 0.075 0.150 0.225 0.300
clone distance sibling distance more distant
  1. 0.177 Gold Cracker (RSP11048)
  2. 0.180 Durban Poison (RSP11014)
  3. 0.192 RKM-2018-006 (RSP11097)
  4. 0.213 RKM-2018-023 (RSP11115)
  5. 0.218 Italian Kiss (RSP11034)
  6. 0.218 UP Sunrise (RSP10989)
  7. 0.223 RKM-2018-020 (RSP11112)
  8. 0.227 RKM-2018-003 (RSP11094)
  9. 0.228 Blue Dream (RSP11033)
  10. 0.242 Blueberry Cheesecake (RSP10684)
  11. 0.244 RKM-2018-031 (RSP11123)
  12. 0.245 CST (RSP11002)
  13. 0.252 Liberty Haze (RSP11000)
  14. 0.254 Golden Goat 2 (RSP10991)
  15. 0.255 Hermaphrodite Research Sample1 (RSP11049)
  16. 0.261 Skunk 18 (RSP11038)
  17. 0.263 RKM-2018-009 (RSP11100)
  18. 0.268 Recon (RSP10755)
  19. 0.270 Cbot-2019-004 (RSP11132)
  20. 0.271 Hermaphrodite ResearchSample2 (RSP11050)

Most genetically distant strains (All Samples)

0 0.125 0.250 0.375 0.500
clone distance sibling distance more distant
  1. 0.474 Cherry Blossom (RSP11323)
  2. 0.440 Cherry Blossom (RSP11311)
  3. 0.433 Cherry Blossom (RSP11328)
  4. 0.429 Unknown- Cherry Wine - 001 (RSP11268)
  5. 0.423 Cherry Blossom (RSP11318)
  6. 0.417 Cherry Blossom (RSP11314)
  7. 0.413 Cherry Blossom (RSP11334)
  8. 0.408 Cherry Blossom (RSP11335)
  9. 0.408 Cherry Blossom (RSP11309)
  10. 0.407 Cherry Blossom (RSP11308)
  11. 0.404 Cherry Blossom (RSP11298)
  12. 0.404 Cherry Blossom (RSP11327)
  13. 0.403 Cherry Blossom (RSP11317)
  14. 0.402 Unknown- Cherry Wine - 002 (RSP11269)
  15. 0.402 Cherry Blossom (RSP11274)
  16. 0.401 Cherry Blossom CBG (RSP11303)
  17. 0.399 Cherry Blossom (RSP11312)
  18. 0.398 Feral (RSP11205)
  19. 0.395 Cherry Blossom (RSP11301)
  20. 0.394 CS (RSP11208)

Most genetically distant strains (Base Tree)

0 0.100 0.200 0.300 0.400
clone distance sibling distance more distant
  1. 0.386 Cbot-2019-005 (RSP11133)
  2. 0.374 Feral (RSP10890)
  3. 0.373 Kush Hemp E1 (RSP11128)
  4. 0.358 Skywalker OG (RSP10837)
  5. 0.355 RKM-2018-034 (RSP11126)
  6. 0.354 Carmagnola (RSP11037)
  7. 0.353 Santhica27 (RSP11047)
  8. 0.352 RKM-2018-026 (RSP11118)
  9. 0.349 Ivory (RSP10668)
  10. 0.347 Futura 75 (RSP10664)
  11. 0.344 Monoica (RSP10241)
  12. 0.342 The Gift (RSP10988)
  13. 0.341 Tisza (RSP11044)
  14. 0.336 USO 31 (RSP10981)
  15. 0.335 RKM-2018-019 (RSP11111)
  16. 0.334 Cherry (RSP11143)
  17. 0.334 Tisza (RSP10659)
  18. 0.333 KYRG-11 (RSP11051)
  19. 0.333 Lovrin (RSP10658)
  20. 0.332 RKM-2018-002 (RSP11093)

Nearest genetic relative in Phylos dataset

Phylos Strain SRR8349002
Overlapping SNPs:
90
Concordance:
77

Nearest genetic relative in Lynch dataset

Lynch Strain SRR3495298
Overlapping SNPs:
13
Concordance:
10

Blockchain Registration Information

Transaction ID
986f258a8f212418d52f38c9b022089b2a54789174f3af1a5ca02a8a32479e12
Stamping Certificate
Download PDF (858.4 KB)
SHASUM Hash
2f4d9e3f3ff59fc6529b7fa99f06c5000159847b8ce40bb183f4554db8db29f2
QR code for RSP11119

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