RKM-2018-028

RSP 11120

Grower: R-Kiem Seeds

General Information

Accession Date
October 21, 2018
Reported Plant Sex
Female

The strain rarity visualization shows how distant the strain is from the other cultivars in the Kannapedia database. The y-axis represents genetic distance, getting farther as you go up. The width of the visualization at any position along the y-axis shows how many strains there are in the database at that genetic distance. So, a common strain will have a more bottom-heavy shape, while uncommon and rare cultivars will have a visualization that is generally shifted towards the top.

Rarity: Rare
Most Distant Most Similar

Chemical Information

Cannabinoid and terpenoid information provided by the grower.

Cannabinoids

No information provided.

Terpenoids

No information provided.

Genetic Information

Plant Type
Type I

The bell curve in the heterozygosity visualization shows the distribution of heterozygosity levels for cannabis cultivars in the Kannapedia database. The green line shows where this particular strain fits within the distribution. Heterozygosity is associated with heterosis (aka hybrid vigor) but also leads to the production of more variable offspring. When plants have two genetically different parents, heterozygosity levels will be higher than if it has been inbred or backcrossed repeatedly.

Heterozygosity: 1.31%
Least Heterozygous Most Heterozygous

The ratio of reads mapped to Y-contigs to reads mapped to the whole Cannabis genome (Y-ratios) has been demonstrated to be strongly correlated with plant sex typing. This plot shows the distribution of Y-ratios for all samples in our database which were sequenced with the same method (panel or WGS) as this sample and where this sample falls in the distribution.

Y-Ratio Distribution: 0.0424
male female RSP11120

This chart represents the Illumina sequence coverage over the Bt/Bd allele. These are the three regions in the cannabis genome that impact THCA, CBDA, CBGA production. Coverage over the Active CBDAS gene is highly correlated with Type II and Type III plants as described by Etienne de Meijer. Coverage over the THCA gene is highly correlated with Type I and Type II plants but is anti-correlated with Type III plants. Type I plants require coverage over the inactive CBDA loci and no coverage over the Active CBDA gene. Lack of coverage over the Active CBDA and Active THCA allele are presumed to be Type IV plants (CBGA dominant). While deletions of entire THCAS and CBDAS genes are the most common Bt:Bd alleles observed, it is possible to have plants with these genes where functional expression of the enzyme is disrupted by deactivating point mutations (Kojoma et al. 2006).

Bt/Bd Allele Coverage

This chart represents the Illumina sequence coverage over the CBCA synthase gene.

CBCAS Coverage

Variants (THCAS, CBDAS, and CBCAS)

Gene HGVS.c HGVS.p Annotation Annotation Impact Contig Contig Pos Ref/Alt Var Freq
THCAS c.749C>A p.Ala250Asp missense variant moderate contig741 4417079

IGV: Start, Jump

G/T
NGS:
0.127
C90:
0.632

Variants (Select Genes of Interest)

PKSG-2a

UniProt

c.67T>A p.Phe23Ile missense variant moderate contig700 1945567

IGV: Start, Jump

A/T
NGS:
0.825
C90:
0.904
PKSG-2a

UniProt

c.31A>T p.Thr11Ser missense variant moderate contig700 1945603

IGV: Start, Jump

T/A
NGS:
0.763
C90:
0.876
PKSG-2b

UniProt

c.1152T>A p.Asn384Lys missense variant moderate contig700 1950486

IGV: Start, Jump

A/T
NGS:
0.715
C90:
0.895
PKSG-2b

UniProt

c.1132C>G p.Leu378Val missense variant moderate contig700 1950506

IGV: Start, Jump

G/C
NGS:
0.717
C90:
0.000
PKSG-2b

UniProt

c.1117A>G p.Ile373Val missense variant moderate contig700 1950521

IGV: Start, Jump

T/C
NGS:
0.807
C90:
0.981
PKSG-2b

UniProt

c.948T>G p.Asp316Glu missense variant moderate contig700 1950690

IGV: Start, Jump

A/C
NGS:
0.456
C90:
0.000
PKSG-2b

UniProt

c.945T>G p.Ser315Arg missense variant moderate contig700 1950693

IGV: Start, Jump

A/C
NGS:
0.454
C90:
0.000
PKSG-2b

UniProt

c.944G>A p.Ser315Asn missense variant moderate contig700 1950694

IGV: Start, Jump

C/T
NGS:
0.445
C90:
0.000
PKSG-2b

UniProt

c.31A>T p.Thr11Ser missense variant moderate contig700 1951851

IGV: Start, Jump

T/A
NGS:
0.844
C90:
0.880
PKSG-4b

UniProt

c.496A>G p.Lys166Glu missense variant moderate contig700 2721177

IGV: Start, Jump

T/C
NGS:
0.651
C90:
0.684
PKSG-4b

UniProt

c.489delT p.Phe163fs frameshift variant high contig700 2721183

IGV: Start, Jump

CA/C
NGS:
0.792
C90:
0.761
PKSG-4b

UniProt

c.485A>G p.Lys162Arg missense variant moderate contig700 2721188

IGV: Start, Jump

T/C
NGS:
0.643
C90:
0.301
PKSG-4b

UniProt

c.431T>G p.Val144Gly missense variant moderate contig700 2721242

IGV: Start, Jump

A/C
NGS:
0.575
C90:
0.679
PKSG-4b

UniProt

c.419A>G p.Asp140Gly missense variant moderate contig700 2721254

IGV: Start, Jump

T/C
NGS:
0.430
C90:
0.440
PKSG-4b

UniProt

c.352_355delACAG p.Thr118fs frameshift variant high contig700 2721317

IGV: Start, Jump

CCTGT/C
NGS:
0.529
C90:
0.000
aPT4

UniProt

c.235_236delGT p.Val79fs frameshift variant high contig121 2829030

IGV: Start, Jump

ATG/A
NGS:
0.417
C90:
0.000
aPT4

UniProt

c.238delT p.Ser80fs frameshift variant high contig121 2829034

IGV: Start, Jump

AT/A
NGS:
0.421
C90:
0.000
aPT4

UniProt

c.302A>G p.Asn101Ser missense variant moderate contig121 2829099

IGV: Start, Jump

A/G
NGS:
0.349
C90:
0.000
aPT4

UniProt

c.1168T>C p.Tyr390His missense variant moderate contig121 2833503

IGV: Start, Jump

T/C
NGS:
0.546
C90:
0.000
aPT1

UniProt

c.406A>G p.Ile136Val missense variant moderate contig121 2839605

IGV: Start, Jump

A/G
NGS:
0.579
C90:
0.761
aPT1

UniProt

c.629C>T p.Thr210Ile missense variant moderate contig121 2840237

IGV: Start, Jump

C/T
NGS:
0.561
C90:
0.598
HDS-2

UniProt

c.82_93delGTAACCGGAACT p.Val28_Thr31del conservative inframe deletion moderate contig95 1989748

IGV: Start, Jump

CGTAACCGGAACT/C
NGS:
0.805
C90:
0.000
HDS-2

UniProt

c.127T>G p.Ser43Ala missense variant moderate contig95 1989794

IGV: Start, Jump

T/G
NGS:
0.721
C90:
0.000

Nearest genetic relatives (All Samples)

0 0.067 0.133 0.200 0.267
clone distance sibling distance more distant
  1. 0.198 RKM-2018-018 (RSP11110)
  2. 0.199 Cheese (RSP10460)
  3. 0.206 RKM-2018-030 (RSP11122)
  4. 0.213 Hermaphrodite Research Sample1 (RSP11049)
  5. 0.214 CPH (RSP11367)
  6. 0.217 Hermaphrodite Research Sample1 (RSP11042)
  7. 0.219 Green Crack (RSP11339)
  8. 0.220 Hindu Kush (SRR14708261)
  9. 0.227 UP Sunrise (RSP10989)
  10. 0.227 RKM-2018-003 (RSP11094)
  11. 0.228 Bordello (RSP11228)
  12. 0.233 TI (RSP11149)
  13. 0.235 T S A G E (RSP11351)
  14. 0.235 Trump x Trump (RSP11466)
  15. 0.237 Glueberry OG (RSP11222)
  16. 0.239 Blue Dream (RSP11017)
  17. 0.239 JL Cross 13 (RSP11514)
  18. 0.243 Jacks Cleaner (RSP11347)
  19. 0.244 Rest (RSP11377)
  20. 0.244 Alpine Rocket (SRR14708266)

Nearest genetic relatives (Base Tree)

0 0.075 0.150 0.225 0.300
clone distance sibling distance more distant
  1. 0.203 RKM-2018-018 (RSP11110)
  2. 0.231 RKM-2018-003 (RSP11094)
  3. 0.232 Hermaphrodite Research Sample1 (RSP11049)
  4. 0.236 UP Sunrise (RSP10989)
  5. 0.246 Sour Raspberry (RSP10551)
  6. 0.255 Cbot-2019-006 (RSP11134)
  7. 0.262 RKM-2018-009 (RSP11100)
  8. 0.265 Liberty Haze (RSP11000)
  9. 0.266 RKM-2018-006 (RSP11097)
  10. 0.268 Italian Kiss (RSP11034)
  11. 0.273 Golden Goat 2 (RSP10991)
  12. 0.274 RKM-2018-022 (RSP11114)
  13. 0.276 Blueberry Cheesecake (RSP10684)
  14. 0.279 Gold Cracker (RSP11048)
  15. 0.280 Blue Dream (RSP11033)
  16. 0.282 Skunk#18 (RSP11038)
  17. 0.282 RKM-2018-020 (RSP11112)
  18. 0.285 RKM-2018-023 (RSP11115)
  19. 0.287 RKM-2018-031 (RSP11123)
  20. 0.291 Durban Poison (RSP11014)

Most genetically distant strains (All Samples)

0 0.125 0.250 0.375 0.500
clone distance sibling distance more distant
  1. 0.483 Cherry Blossom (RSP11317)
  2. 0.461 Cherry Blossom (RSP11311)
  3. 0.450 Cherry Blossom (RSP11314)
  4. 0.449 Cherry Blossom (RSP11298)
  5. 0.444 Cherry Blossom (RSP11328)
  6. 0.439 Cherry Blossom (RSP11330)
  7. 0.439 Cherry Blossom (RSP11309)
  8. 0.431 Tiger Tail -30- (RSP11484)
  9. 0.425 80E (RSP11213)
  10. 0.425 Cherry Blossom (RSP11334)
  11. 0.423 Right Mark (RSP11628)
  12. 0.422 Cherry Blossom (RSP11324)
  13. 0.421 Cherry Blossom (RSP11326)
  14. 0.421 Feral (RSP11205)
  15. 0.420 80E (RSP11211)
  16. 0.417 CS Indica (RSP11658)
  17. 0.416 Feral (RSP10892)
  18. 0.416 Cherry Blossom (RSP11333)
  19. 0.415 R1 (RSP11483)
  20. 0.415 Feral (RSP11206)

Most genetically distant strains (Base Tree)

0 0.108 0.217 0.325 0.433
clone distance sibling distance more distant
  1. 0.410 Feral (RSP10890)
  2. 0.402 RKM-2018-026 (RSP11118)
  3. 0.400 Cherry (RSP11142)
  4. 0.393 RKM-2018-034 (RSP11126)
  5. 0.392 JL yellow (RSP11075)
  6. 0.391 Skywalker OG (RSP10837)
  7. 0.390 Monoica (RSP10241)
  8. 0.386 Carmagnola (RSP11037)
  9. 0.381 Kyrgyz Gold (RSP11054)
  10. 0.378 Cbot-2019-005 (RSP11133)
  11. 0.377 Tisza (RSP11044)
  12. 0.373 Santhica27 (RSP11047)
  13. 0.372 USO 31 (RSP10981)
  14. 0.370 Jiangji (RSP10653)
  15. 0.368 Futura 75 (RSP10664)
  16. 0.368 Fedora 17 (RSP10661)
  17. 0.367 Tisza (RSP10659)
  18. 0.365 Carmagnola (RSP10979)
  19. 0.364 Lovrin (RSP10658)
  20. 0.361 Ivory (RSP10668)

Nearest genetic relative in Phylos dataset

Phylos Strain SRR4448319
Overlapping SNPs:
95
Concordance:
65

Nearest genetic relative in Lynch dataset

Lynch Strain SRR3495250
Overlapping SNPs:
3
Concordance:
3

Blockchain Registration Information

Transaction ID
8df1baad0dd4e0bcb0fb051b0cd805f9e8a669d18f4f2d7ecce231eaa4a0381f
Stamping Certificate
Download PDF (860.7 KB)
SHASUM Hash
7b903d28fac4997ac223c5b1d1581ff694e5f9b9c1518da2d8e30397b98a2d21
QR code for RSP11120

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