RKM-2018-029

RSP 11121

Grower: R-Kiem Seeds

General Information

Accession Date
October 21, 2018
Reported Plant Sex
Male

The strain rarity visualization shows how distant the strain is from the other cultivars in the Kannapedia database. The y-axis represents genetic distance, getting farther as you go up. The width of the visualization at any position along the y-axis shows how many strains there are in the database at that genetic distance. So, a common strain will have a more bottom-heavy shape, while uncommon and rare cultivars will have a visualization that is generally shifted towards the top.

Rarity: Rare
Most Distant Most Similar

Chemical Information

Cannabinoid and terpenoid information provided by the grower.

Cannabinoids

No information provided.

Terpenoids

No information provided.

Genetic Information

Plant Type
Type I

The bell curve in the heterozygosity visualization shows the distribution of heterozygosity levels for cannabis cultivars in the Kannapedia database. The green line shows where this particular strain fits within the distribution. Heterozygosity is associated with heterosis (aka hybrid vigor) but also leads to the production of more variable offspring. When plants have two genetically different parents, heterozygosity levels will be higher than if it has been inbred or backcrossed repeatedly.

Heterozygosity: 2.1%
Least Heterozygous Most Heterozygous

The ratio of reads mapped to Y-contigs to reads mapped to the whole Cannabis genome (Y-ratios) has been demonstrated to be strongly correlated with plant sex typing. This plot shows the distribution of Y-ratios for all samples in our database which were sequenced with the same method (panel or WGS) as this sample and where this sample falls in the distribution.

Y-Ratio Distribution: 0.0977
male female RSP11121

This chart represents the Illumina sequence coverage over the Bt/Bd allele. These are the three regions in the cannabis genome that impact THCA, CBDA, CBGA production. Coverage over the Active CBDAS gene is highly correlated with Type II and Type III plants as described by Etienne de Meijer. Coverage over the THCA gene is highly correlated with Type I and Type II plants but is anti-correlated with Type III plants. Type I plants require coverage over the inactive CBDA loci and no coverage over the Active CBDA gene. Lack of coverage over the Active CBDA and Active THCA allele are presumed to be Type IV plants (CBGA dominant). While deletions of entire THCAS and CBDAS genes are the most common Bt:Bd alleles observed, it is possible to have plants with these genes where functional expression of the enzyme is disrupted by deactivating point mutations (Kojoma et al. 2006).

Bt/Bd Allele Coverage

This chart represents the Illumina sequence coverage over the CBCA synthase gene.

CBCAS Coverage

Variants (THCAS, CBDAS, and CBCAS)

THCAS c.998C>G p.Pro333Arg missense variant moderate contig741 4416830

IGV: Start, Jump

G/C
NGS:
0.182
C90:
0.000
THCAS c.922A>G p.Thr308Ala missense variant moderate contig741 4416906

IGV: Start, Jump

T/C
NGS:
0.004
C90:
0.000
THCAS c.373G>C p.Val125Leu missense variant moderate contig741 4417455

IGV: Start, Jump

C/G
NGS:
0.050
C90:
0.000

Variants (Select Genes of Interest)

GPPs1

UniProt

c.160A>G p.Lys54Glu missense variant moderate contig676 168409

IGV: Start, Jump

A/G
NGS:
0.026
C90:
0.000
GPPs1

UniProt

c.472T>A p.Leu158Met missense variant moderate contig676 168721

IGV: Start, Jump

T/A
NGS:
0.020
C90:
0.000
GPPs1

UniProt

c.807_814delGCATTTTT p.His270fs frameshift variant high contig676 169595

IGV: Start, Jump

GTGCATTTT/G
NGS:
0.015
C90:
0.000
PKSG-2a

UniProt

c.67T>G p.Phe23Val missense variant moderate contig700 1945567

IGV: Start, Jump

A/C
NGS:
0.002
C90:
0.000
PKSG-2a

UniProt

c.67T>A p.Phe23Ile missense variant moderate contig700 1945567

IGV: Start, Jump

A/T
NGS:
0.825
C90:
0.904
PKSG-2a

UniProt

c.31A>T p.Thr11Ser missense variant moderate contig700 1945603

IGV: Start, Jump

T/A
NGS:
0.763
C90:
0.876
PKSG-2b

UniProt

c.1152T>A p.Asn384Lys missense variant moderate contig700 1950486

IGV: Start, Jump

A/T
NGS:
0.715
C90:
0.895
PKSG-2b

UniProt

c.1132C>G p.Leu378Val missense variant moderate contig700 1950506

IGV: Start, Jump

G/C
NGS:
0.717
C90:
0.000
PKSG-2b

UniProt

c.1117A>G p.Ile373Val missense variant moderate contig700 1950521

IGV: Start, Jump

T/C
NGS:
0.807
C90:
0.981
PKSG-2b

UniProt

c.774G>A p.Met258Ile missense variant moderate contig700 1950864

IGV: Start, Jump

C/T
NGS:
0.366
C90:
0.947
PKSG-2b

UniProt

c.31A>T p.Thr11Ser missense variant moderate contig700 1951851

IGV: Start, Jump

T/A
NGS:
0.844
C90:
0.880
PKSG-2b

UniProt

c.-2_1dupATA start lost & conservative inframe insertion high contig700 1951880

IGV: Start, Jump

A/ATAT
NGS:
0.410
C90:
0.000
PKSG-4b

UniProt

c.496A>G p.Lys166Glu missense variant moderate contig700 2721177

IGV: Start, Jump

T/C
NGS:
0.651
C90:
0.684
PKSG-4b

UniProt

c.489delT p.Phe163fs frameshift variant high contig700 2721183

IGV: Start, Jump

CA/C
NGS:
0.792
C90:
0.761
PKSG-4b

UniProt

c.485A>G p.Lys162Arg missense variant moderate contig700 2721188

IGV: Start, Jump

T/C
NGS:
0.643
C90:
0.301
PKSG-4b

UniProt

c.431T>G p.Val144Gly missense variant moderate contig700 2721242

IGV: Start, Jump

A/C
NGS:
0.575
C90:
0.679
PKSG-4b

UniProt

c.419A>G p.Asp140Gly missense variant moderate contig700 2721254

IGV: Start, Jump

T/C
NGS:
0.430
C90:
0.440
PKSG-4b

UniProt

c.352_355delACAG p.Thr118fs frameshift variant high contig700 2721317

IGV: Start, Jump

CCTGT/C
NGS:
0.529
C90:
0.000
PKSG-4b

UniProt

c.353_354insCC p.Gly119fs frameshift variant high contig700 2721319

IGV: Start, Jump

T/TGG
NGS:
0.175
C90:
0.000
PKSG-4b

UniProt

c.323A>G p.Glu108Gly missense variant moderate contig700 2721350

IGV: Start, Jump

T/C
NGS:
0.469
C90:
0.000
DXR-2

UniProt

c.1319T>C p.Ile440Thr missense variant moderate contig380 285250

IGV: Start, Jump

A/G
NGS:
0.480
C90:
0.000
aPT4

UniProt

c.35A>C p.Gln12Pro missense variant moderate contig121 2828691

IGV: Start, Jump

A/C
NGS:
0.075
C90:
0.000
aPT4

UniProt

c.235_236delGT p.Val79fs frameshift variant high contig121 2829030

IGV: Start, Jump

ATG/A
NGS:
0.417
C90:
0.000
aPT4

UniProt

c.238delT p.Ser80fs frameshift variant high contig121 2829034

IGV: Start, Jump

AT/A
NGS:
0.421
C90:
0.000
aPT4

UniProt

c.450G>A p.Met150Ile missense variant moderate contig121 2830645

IGV: Start, Jump

G/A
NGS:
0.007
C90:
0.000
aPT1

UniProt

c.406A>G p.Ile136Val missense variant moderate contig121 2839605

IGV: Start, Jump

A/G
NGS:
0.579
C90:
0.761
HDS-2

UniProt

c.82_93delGTAACCGGAACT p.Val28_Thr31del conservative inframe deletion moderate contig95 1989748

IGV: Start, Jump

CGTAACCGGAACT/C
NGS:
0.805
C90:
0.000
HDS-2

UniProt

c.127T>G p.Ser43Ala missense variant moderate contig95 1989794

IGV: Start, Jump

T/G
NGS:
0.721
C90:
0.000

Nearest genetic relatives (All Samples)

0 0.067 0.133 0.200 0.267
clone distance sibling distance more distant
  1. 0.125 RKM-2018-015 (RSP11107)
  2. 0.222 Blue Dream (RSP11017)
  3. 0.228 Serious Happiness (RSP10763)
  4. 0.232 Blue Dream (RSP11006)
  5. 0.234 Golden Goat 2 (RSP10991)
  6. 0.235 Domnesia (RSP11184)
  7. 0.237 Blue Dream (RSP11009)
  8. 0.238 Super Blue Dream (RSP11011)
  9. 0.238 Blue Dream (RSP11010)
  10. 0.240 Blueberry Cheesecake (RSP10684)
  11. 0.240 Snoops Dream (RSP11031)
  12. 0.240 Blue Dream (RSP11008)
  13. 0.242 Golden Road (RSP11345)
  14. 0.242 Blue Dream (RSP11007)
  15. 0.244 NSPM1 (RSP11362)
  16. 0.244 Durban Poison 1 (RSP11013)
  17. 0.245 Snoops Dream (RSP11003)
  18. 0.245 Liberty Haze (RSP11000)
  19. 0.246 Blue Dream (RSP11004)
  20. 0.246 Blue Dream (RSP11012)

Nearest genetic relatives (Base Tree)

0 0.075 0.150 0.225 0.300
clone distance sibling distance more distant
  1. 0.235 Golden Goat 2 (RSP10991)
  2. 0.239 Blueberry Cheesecake (RSP10684)
  3. 0.249 Liberty Haze (RSP11000)
  4. 0.249 Durban Poison (RSP11014)
  5. 0.251 RKM-2018-033 (RSP11125)
  6. 0.256 RKM-2018-005 (RSP11096)
  7. 0.257 Sour Raspberry (RSP10551)
  8. 0.258 Blue Dream (RSP11033)
  9. 0.258 Blueberry Cheesecake (RSP10680)
  10. 0.259 Gold Cracker (RSP11048)
  11. 0.259 CST (RSP11002)
  12. 0.263 RKM-2018-032 (RSP11124)
  13. 0.263 RKM-2018-023 (RSP11115)
  14. 0.263 Hermaphrodite Research Sample1 (RSP11049)
  15. 0.263 Cbot-2019-006 (RSP11134)
  16. 0.265 RKM-2018-003 (RSP11094)
  17. 0.265 Hermaphrodite ResearchSample2 (RSP11050)
  18. 0.270 Kimbo Slice (RSP10997)
  19. 0.272 Recon (RSP10755)
  20. 0.273 RKM-2018-031 (RSP11123)

Most genetically distant strains (All Samples)

0 0.117 0.233 0.350 0.467
clone distance sibling distance more distant
  1. 0.442 Cherry Blossom (RSP11323)
  2. 0.436 Cherry Blossom (RSP11318)
  3. 0.435 Cherry Blossom (RSP11334)
  4. 0.434 Cherry Blossom (RSP11311)
  5. 0.432 Unknown- Cherry Wine - 001 (RSP11268)
  6. 0.422 Cherry Blossom (RSP11333)
  7. 0.419 Cherry Blossom (RSP11314)
  8. 0.416 Cherry Blossom (RSP11324)
  9. 0.414 Cherry Blossom (RSP11301)
  10. 0.413 Cherry Blossom (RSP11328)
  11. 0.405 Cherry Blossom (RSP11300)
  12. 0.404 Unknown- Cherry Wine - 002 (RSP11269)
  13. 0.403 Cbot-2019-005 (RSP11133)
  14. 0.402 Cherry Blossom (RSP11274)
  15. 0.399 Cherry Blossom (RSP11325)
  16. 0.398 Cherry Blossom (RSP11331)
  17. 0.397 Cherry Blossom (RSP11308)
  18. 0.395 Unknown- Cherry Wine - 004 (RSP11271)
  19. 0.393 80E (RSP11213)
  20. 0.393 Cherry Blossom (RSP11306)

Most genetically distant strains (Base Tree)

0 0.108 0.217 0.325 0.433
clone distance sibling distance more distant
  1. 0.408 Cbot-2019-005 (RSP11133)
  2. 0.360 Kush Hemp E1 (RSP11128)
  3. 0.339 Cherry (RSP11142)
  4. 0.336 Cbot-2019-004 (RSP11132)
  5. 0.332 Cherry (RSP11143)
  6. 0.332 JL yellow (RSP11075)
  7. 0.331 RKM-2018-006 (RSP11097)
  8. 0.330 Cbot-2019-001 (RSP11129)
  9. 0.325 Carmagnola (RSP11037)
  10. 0.323 Black Beauty (RSP11035)
  11. 0.319 RKM-2018-022 (RSP11114)
  12. 0.318 RKM-2018-034 (RSP11126)
  13. 0.317 Santhica27 (RSP11047)
  14. 0.316 Carmagnola (RSP10979)
  15. 0.311 RKM-2018-002 (RSP11093)
  16. 0.308 RKM-2018-026 (RSP11118)
  17. 0.308 Fedora 17 (RSP10661)
  18. 0.307 Monoica (RSP10241)
  19. 0.306 Feral (RSP10890)
  20. 0.306 Ivory (RSP10668)

Nearest genetic relative in Phylos dataset

Phylos Strain SRR8346886
Overlapping SNPs:
91
Concordance:
58

Nearest genetic relative in Lynch dataset

Lynch Strain SRR3495196
Overlapping SNPs:
3
Concordance:
3

Blockchain Registration Information

Transaction ID
ed55a07609d6a2e97fbf3298dce345a368efd777aa64d915ec856c0bce9966fe
Stamping Certificate
Download PDF (868.0 KB)
SHASUM Hash
b5dfaf6100b6d6e2f49090166c89860af8b50bc0d79455a2553b9416564a6ae7
QR code for RSP11121

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