Cbot-2019-001

RSP 11129

Grower: Research Development Cannabinoid

General Information

Accession Date
February 5, 2019
Reported Plant Sex
Female
DNA Extracted From
Stem

The strain rarity visualization shows how distant the strain is from the other cultivars in the Kannapedia database. The y-axis represents genetic distance, getting farther as you go up. The width of the visualization at any position along the y-axis shows how many strains there are in the database at that genetic distance. So, a common strain will have a more bottom-heavy shape, while uncommon and rare cultivars will have a visualization that is generally shifted towards the top.

Rarity: Uncommon
Most Distant Most Similar

Chemical Information

Cannabinoid and terpenoid information provided by the grower.

Cannabinoids

No information provided.

Terpenoids

No information provided.

Genetic Information

Plant Type
Type II

The bell curve in the heterozygosity visualization shows the distribution of heterozygosity levels for cannabis cultivars in the Kannapedia database. The green line shows where this particular strain fits within the distribution. Heterozygosity is associated with heterosis (aka hybrid vigor) but also leads to the production of more variable offspring. When plants have two genetically different parents, heterozygosity levels will be higher than if it has been inbred or backcrossed repeatedly.

Heterozygosity: 0.84%
Least Heterozygous Most Heterozygous

The ratio of reads mapped to Y-contigs to reads mapped to the whole Cannabis genome (Y-ratios) has been demonstrated to be strongly correlated with plant sex typing. This plot shows the distribution of Y-ratios for all samples in our database which were sequenced with the same method (panel or WGS) as this sample and where this sample falls in the distribution.

Y-Ratio Distribution: 0.0380
male female RSP11129

This chart represents the Illumina sequence coverage over the Bt/Bd allele. These are the three regions in the cannabis genome that impact THCA, CBDA, CBGA production. Coverage over the Active CBDAS gene is highly correlated with Type II and Type III plants as described by Etienne de Meijer. Coverage over the THCA gene is highly correlated with Type I and Type II plants but is anti-correlated with Type III plants. Type I plants require coverage over the inactive CBDA loci and no coverage over the Active CBDA gene. Lack of coverage over the Active CBDA and Active THCA allele are presumed to be Type IV plants (CBGA dominant). While deletions of entire THCAS and CBDAS genes are the most common Bt:Bd alleles observed, it is possible to have plants with these genes where functional expression of the enzyme is disrupted by deactivating point mutations (Kojoma et al. 2006).

Bt/Bd Allele Coverage

This chart represents the Illumina sequence coverage over the CBCA synthase gene.

CBCAS Coverage

Variants (THCAS, CBDAS, and CBCAS)

No variants to report

Variants (Select Genes of Interest)

PKSG-2a

UniProt

c.67T>A p.Phe23Ile missense variant moderate contig700 1945567

IGV: Start, Jump

A/T
NGS:
0.825
C90:
0.904
PKSG-2a

UniProt

c.31A>T p.Thr11Ser missense variant moderate contig700 1945603

IGV: Start, Jump

T/A
NGS:
0.763
C90:
0.876
PKSG-2b

UniProt

c.1152T>A p.Asn384Lys missense variant moderate contig700 1950486

IGV: Start, Jump

A/T
NGS:
0.715
C90:
0.895
PKSG-2b

UniProt

c.1132C>G p.Leu378Val missense variant moderate contig700 1950506

IGV: Start, Jump

G/C
NGS:
0.717
C90:
0.000
PKSG-2b

UniProt

c.1117A>G p.Ile373Val missense variant moderate contig700 1950521

IGV: Start, Jump

T/C
NGS:
0.807
C90:
0.981
PKSG-2b

UniProt

c.948T>G p.Asp316Glu missense variant moderate contig700 1950690

IGV: Start, Jump

A/C
NGS:
0.456
C90:
0.000
PKSG-2b

UniProt

c.945T>G p.Ser315Arg missense variant moderate contig700 1950693

IGV: Start, Jump

A/C
NGS:
0.454
C90:
0.000
PKSG-2b

UniProt

c.31A>T p.Thr11Ser missense variant moderate contig700 1951851

IGV: Start, Jump

T/A
NGS:
0.844
C90:
0.880
PKSG-4b

UniProt

c.496A>G p.Lys166Glu missense variant moderate contig700 2721177

IGV: Start, Jump

T/C
NGS:
0.651
C90:
0.684
PKSG-4b

UniProt

c.492G>C p.Glu164Asp missense variant moderate contig700 2721181

IGV: Start, Jump

CTCA/GTCA
NGS:
0.048
C90:
0.000
PKSG-4b

UniProt

c.489_491delTGA p.Phe163_Glu164delinsLeu disruptive inframe deletion moderate contig700 2721181

IGV: Start, Jump

CTCA/C
NGS:
0.002
C90:
0.000
PKSG-4b

UniProt

c.489delT p.Phe163fs frameshift variant high contig700 2721183

IGV: Start, Jump

CA/C
NGS:
0.792
C90:
0.761
PKSG-4b

UniProt

c.485A>G p.Lys162Arg missense variant moderate contig700 2721188

IGV: Start, Jump

T/C
NGS:
0.643
C90:
0.301
PKSG-4b

UniProt

c.431T>G p.Val144Gly missense variant moderate contig700 2721242

IGV: Start, Jump

A/C
NGS:
0.575
C90:
0.679
PKSG-4b

UniProt

c.419A>G p.Asp140Gly missense variant moderate contig700 2721254

IGV: Start, Jump

T/C
NGS:
0.430
C90:
0.440
aPT4

UniProt

c.1168T>C p.Tyr390His missense variant moderate contig121 2833503

IGV: Start, Jump

T/C
NGS:
0.546
C90:
0.000
aPT1

UniProt

c.629C>T p.Thr210Ile missense variant moderate contig121 2840237

IGV: Start, Jump

C/T
NGS:
0.561
C90:
0.598
HDS-2

UniProt

c.82_93delGTAACCGGAACT p.Val28_Thr31del conservative inframe deletion moderate contig95 1989748

IGV: Start, Jump

CGTAACCGGAACT/C
NGS:
0.805
C90:
0.000
HDS-2

UniProt

c.127T>G p.Ser43Ala missense variant moderate contig95 1989794

IGV: Start, Jump

T/G
NGS:
0.721
C90:
0.000

Nearest genetic relatives (All Samples)

0 0.058 0.117 0.175 0.233
clone distance sibling distance more distant
  1. 0.188 AVIDEKEL 2 0 (RSP11174)
  2. 0.189 101st AIRBORN CBD (RSP11350)
  3. 0.193 Queen Dream (RSP11291)
  4. 0.195 Wife (RSP11148)
  5. 0.195 Queen Dream CBG (RSP11296)
  6. 0.197 CANNATONIC (RSP11349)
  7. 0.198 Queen Dream CBG (RSP11279)
  8. 0.199 Electra (RSP11366)
  9. 0.199 Queen Dream CBG (RSP11297)
  10. 0.204 Hot Blonde (RSP11292)
  11. 0.205 Queen Dream CBG (RSP11276)
  12. 0.208 Queen Dream CBG (RSP11285)
  13. 0.208 Medxotic (RSP11410)
  14. 0.209 Ringo s Gift -Katie s Cut- (RSP11624)
  15. 0.212 Cbot-2019-003 (RSP11131)
  16. 0.215 Queen Dream CBG (RSP11283)
  17. 0.217 QLF4 (RSP11441)
  18. 0.220 Queen Dream CBG (RSP11293)
  19. 0.221 Chematonic -Cannatonic x Chemdawg- (RSP11394)
  20. 0.222 Liberty Haze (RSP11000)

Most genetically distant strains (All Samples)

0 0.117 0.233 0.350 0.467
clone distance sibling distance more distant
  1. 0.439 GMO x Zkittlez #43 (RSP11976)
  2. 0.438 Northern Skunk (RSP11456)
  3. 0.435 80E (RSP11211)
  4. 0.432 80E (RSP11213)
  5. 0.427 Star Dawg (RSP11352)
  6. 0.422 GMO x Garlic Breath (RSP12507)
  7. 0.419 Fatso (RSP11741)
  8. 0.416 Cherry Blossom (RSP11334)
  9. 0.415 BagSeed (RSP12501)
  10. 0.415 CHEM4 (RSP12090)
  11. 0.412 Ruderalis Indica (SRR14708267)
  12. 0.412 BagSeed (RSP12627)
  13. 0.411 Northern Lights (RSP11501)
  14. 0.409 80E (RSP11212)
  15. 0.406 JL 3rd Gen Father (RSP11196)
  16. 0.403 New York City Deisel (RSP11225)
  17. 0.403 Juicy Gummy x Royal Kush (RSP12484)
  18. 0.402 Right Mark (RSP11628)
  19. 0.400 RKM-2018-026 (RSP11118)
  20. 0.399 Cherry Blossom (RSP11333)

Nearest genetic relative in Phylos dataset

Phylos Strain SRR4448694
Overlapping SNPs:
122
Concordance:
100

Nearest genetic relative in Lynch dataset

Lynch Strain SRR3495250
Overlapping SNPs:
3
Concordance:
3

Blockchain Registration Information

Transaction ID
899d461f0a098ea22ce20254a16b2073e04b1ce434c355bef00708e8ea6fea8a
Stamping Certificate
Download PDF (862.1 KB)
SHASUM Hash
cd9022391af50374119bb57964d3326744b7a83b5d79a23901e5295d417688e7
QR code for RSP11129

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