Cbot-2019-003

RSP 11131

Grower: Research Development Cannabinoid

General Information

Accession Date
February 5, 2019
Reported Plant Sex
not reported

The strain rarity visualization shows how distant the strain is from the other cultivars in the Kannapedia database. The y-axis represents genetic distance, getting farther as you go up. The width of the visualization at any position along the y-axis shows how many strains there are in the database at that genetic distance. So, a common strain will have a more bottom-heavy shape, while uncommon and rare cultivars will have a visualization that is generally shifted towards the top.

Rarity: Uncommon
Most Distant Most Similar

Chemical Information

Cannabinoid and terpenoid information provided by the grower.

Cannabinoids

No information provided.

Terpenoids

No information provided.

Genetic Information

Plant Type
Type III

The bell curve in the heterozygosity visualization shows the distribution of heterozygosity levels for cannabis cultivars in the Kannapedia database. The green line shows where this particular strain fits within the distribution. Heterozygosity is associated with heterosis (aka hybrid vigor) but also leads to the production of more variable offspring. When plants have two genetically different parents, heterozygosity levels will be higher than if it has been inbred or backcrossed repeatedly.

Heterozygosity: 0.91%
Least Heterozygous Most Heterozygous

The ratio of reads mapped to Y-contigs to reads mapped to the whole Cannabis genome (Y-ratios) has been demonstrated to be strongly correlated with plant sex typing. This plot shows the distribution of Y-ratios for all samples in our database which were sequenced with the same method (panel or WGS) as this sample and where this sample falls in the distribution.

Y-Ratio Distribution: 0.0406
male female RSP11131

This chart represents the Illumina sequence coverage over the Bt/Bd allele. These are the three regions in the cannabis genome that impact THCA, CBDA, CBGA production. Coverage over the Active CBDAS gene is highly correlated with Type II and Type III plants as described by Etienne de Meijer. Coverage over the THCA gene is highly correlated with Type I and Type II plants but is anti-correlated with Type III plants. Type I plants require coverage over the inactive CBDA loci and no coverage over the Active CBDA gene. Lack of coverage over the Active CBDA and Active THCA allele are presumed to be Type IV plants (CBGA dominant). While deletions of entire THCAS and CBDAS genes are the most common Bt:Bd alleles observed, it is possible to have plants with these genes where functional expression of the enzyme is disrupted by deactivating point mutations (Kojoma et al. 2006).

Bt/Bd Allele Coverage

This chart represents the Illumina sequence coverage over the CBCA synthase gene.

CBCAS Coverage

Variants (THCAS, CBDAS, and CBCAS)

No variants to report

Variants (Select Genes of Interest)

PKSG-2a

UniProt

c.67T>A p.Phe23Ile missense variant moderate contig700 1945567

IGV: Start, Jump

A/T
NGS:
0.825
C90:
0.904
PKSG-2a

UniProt

c.31A>T p.Thr11Ser missense variant moderate contig700 1945603

IGV: Start, Jump

T/A
NGS:
0.763
C90:
0.876
PKSG-2b

UniProt

c.1152T>A p.Asn384Lys missense variant moderate contig700 1950486

IGV: Start, Jump

A/T
NGS:
0.715
C90:
0.895
PKSG-2b

UniProt

c.1132C>G p.Leu378Val missense variant moderate contig700 1950506

IGV: Start, Jump

G/C
NGS:
0.717
C90:
0.000
PKSG-2b

UniProt

c.1117A>G p.Ile373Val missense variant moderate contig700 1950521

IGV: Start, Jump

T/C
NGS:
0.807
C90:
0.981
PKSG-2b

UniProt

c.948T>G p.Asp316Glu missense variant moderate contig700 1950690

IGV: Start, Jump

A/C
NGS:
0.456
C90:
0.000
PKSG-2b

UniProt

c.945T>G p.Ser315Arg missense variant moderate contig700 1950693

IGV: Start, Jump

A/C
NGS:
0.454
C90:
0.000
PKSG-2b

UniProt

c.944G>A p.Ser315Asn missense variant moderate contig700 1950694

IGV: Start, Jump

C/T
NGS:
0.445
C90:
0.000
PKSG-2b

UniProt

c.934C>G p.His312Asp missense variant moderate contig700 1950704

IGV: Start, Jump

G/C
NGS:
0.410
C90:
0.000
PKSG-2b

UniProt

c.31A>T p.Thr11Ser missense variant moderate contig700 1951851

IGV: Start, Jump

T/A
NGS:
0.844
C90:
0.880
PKSG-2b

UniProt

c.-2_1dupATA start lost & conservative inframe insertion high contig700 1951880

IGV: Start, Jump

A/ATAT
NGS:
0.410
C90:
0.000
PKSG-4b

UniProt

c.496A>G p.Lys166Glu missense variant moderate contig700 2721177

IGV: Start, Jump

T/C
NGS:
0.651
C90:
0.684
PKSG-4b

UniProt

c.492G>C p.Glu164Asp missense variant moderate contig700 2721181

IGV: Start, Jump

C/G
NGS:
0.048
C90:
0.000
PKSG-4b

UniProt

c.489delT p.Phe163fs frameshift variant high contig700 2721183

IGV: Start, Jump

CA/C
NGS:
0.792
C90:
0.761
PKSG-4b

UniProt

c.485A>G p.Lys162Arg missense variant moderate contig700 2721188

IGV: Start, Jump

T/C
NGS:
0.643
C90:
0.301
PKSG-4b

UniProt

c.431T>G p.Val144Gly missense variant moderate contig700 2721242

IGV: Start, Jump

A/C
NGS:
0.575
C90:
0.679
PKSG-4b

UniProt

c.419A>G p.Asp140Gly missense variant moderate contig700 2721254

IGV: Start, Jump

T/C
NGS:
0.430
C90:
0.440
aPT1

UniProt

c.629C>T p.Thr210Ile missense variant moderate contig121 2840237

IGV: Start, Jump

C/T
NGS:
0.561
C90:
0.598
HDS-2

UniProt

c.82_93delGTAACCGGAACT p.Val28_Thr31del conservative inframe deletion moderate contig95 1989748

IGV: Start, Jump

CGTAACCGGAACT/C
NGS:
0.805
C90:
0.000

Nearest genetic relatives (All Samples)

0 0.058 0.117 0.175 0.233
clone distance sibling distance more distant
  1. 0.119 101st AIRBORN CBD (RSP11350)
  2. 0.186 Cbot-2019-002 (RSP11130)
  3. 0.192 Midnight 05MAY2017 (RSP10941)
  4. 0.193 Suver Haze (RSP11364)
  5. 0.201 CANNATONIC (RSP11349)
  6. 0.204 RAPHAEL #2 (RSP11172)
  7. 0.204 Electra (RSP11366)
  8. 0.210 Cherry Wine (RSP11307)
  9. 0.211 Lifter (RSP11365)
  10. 0.212 Cbot-2019-001 (RSP11129)
  11. 0.212 Cherry Wine (RSP11305)
  12. 0.213 Cbot-2019-005 (RSP11133)
  13. 0.218 Cbot-2019-004 (RSP11132)
  14. 0.219 Joy (RSP11380)
  15. 0.219 Avidekel 05MAY2017 (RSP10938)
  16. 0.220 Unknown--Cherry Wine---005- (RSP11272)
  17. 0.221 Cherry Blossom (RSP11320)
  18. 0.227 AVIDEKEL USA (RSP11169)
  19. 0.229 Lift (RSP11378)
  20. 0.232 Alaska 05MAY2017 (RSP10939)

Nearest genetic relatives (Base Tree)

0 0.092 0.183 0.275 0.367
clone distance sibling distance more distant
  1. 0.217 Cbot-2019-001 (RSP11129)
  2. 0.220 Cbot-2019-005 (RSP11133)
  3. 0.226 Cbot-2019-004 (RSP11132)
  4. 0.244 Liberty Haze (RSP11000)
  5. 0.278 Blueberry Cheesecake (RSP10684)
  6. 0.283 Blueberry Cheesecake (RSP10672)
  7. 0.291 Cherry (RSP11143)
  8. 0.296 Queen Jesus (RSP10105)
  9. 0.302 UP Sunrise (RSP10989)
  10. 0.305 Durban Poison (RSP11014)
  11. 0.314 RKM-2018-031 (RSP11123)
  12. 0.319 CST (RSP11002)
  13. 0.324 Recon (RSP10755)
  14. 0.333 Hermaphrodite ResearchSample2 (RSP11050)
  15. 0.333 RKM-2018-020 (RSP11112)
  16. 0.334 Blueberry Cheesecake (RSP10680)
  17. 0.335 RKM-2018-003 (RSP11094)
  18. 0.336 RKM-2018-004 (RSP11096)
  19. 0.339 RKM-2018-006 (RSP11097)
  20. 0.340 Kimbo Slice (RSP10997)

Most genetically distant strains (All Samples)

0 0.108 0.217 0.325 0.433
clone distance sibling distance more distant
  1. 0.422 GMO x Garlic Breath (RSP12507)
  2. 0.420 GG4 (RSP11978)
  3. 0.420 Fatso (RSP11741)
  4. 0.418 80E (RSP11213)
  5. 0.417 JL yellow (RSP11075)
  6. 0.417 Northern Skunk (RSP11456)
  7. 0.414 White Label 1 (RSP11336)
  8. 0.412 Chem 91 (RSP11185)
  9. 0.412 JL 3rd Gen Mother (RSP11214)
  10. 0.409 CHEM4 (RSP12090)
  11. 0.408 BagSeed (RSP12501)
  12. 0.405 Star Dawg (RSP11352)
  13. 0.403 New York City Deisel (RSP11225)
  14. 0.401 GMO (RSP12091)
  15. 0.401 80E (RSP11211)
  16. 0.401 R1in136 (SRR14708226)
  17. 0.399 Big Bud (SRR14708270)
  18. 0.399 BagSeed (RSP12627)
  19. 0.399 UP Wendigo (RSP11261)
  20. 0.399 JL 3rd Gen Mother (RSP11197)

Most genetically distant strains (Base Tree)

0 0.108 0.217 0.325 0.433
clone distance sibling distance more distant
  1. 0.420 JL yellow (RSP11075)
  2. 0.404 The Gift (RSP10988)
  3. 0.399 RKM-2018-026 (RSP11118)
  4. 0.398 RKM-2018-022 (RSP11114)
  5. 0.395 RKM-2018-023 (RSP11115)
  6. 0.388 Skywalker OG (RSP10837)
  7. 0.386 RKM-2018-034 (RSP11126)
  8. 0.384 RKM-2018-002 (RSP11093)
  9. 0.384 RKM-2018-032 (RSP11124)
  10. 0.382 Italian Kiss (RSP11034)
  11. 0.380 Hermaphrodite Research Sample1 (RSP11049)
  12. 0.379 Kyrgyz Gold (RSP11054)
  13. 0.378 RKM-2018-009 (RSP11100)
  14. 0.378 RKM-2018-028 (RSP11120)
  15. 0.377 Skunk#18 (RSP11038)
  16. 0.377 Monoica (RSP10241)
  17. 0.375 RKM-2018-018 (RSP11110)
  18. 0.374 Carmagnola (RSP11037)
  19. 0.374 Santhica27 (RSP11047)
  20. 0.373 Kush Hemp E1 (RSP11128)

Nearest genetic relative in Phylos dataset

Phylos Strain SRR8346935
Overlapping SNPs:
122
Concordance:
89

Nearest genetic relative in Lynch dataset

Lynch Strain SRR3495181
Overlapping SNPs:
14
Concordance:
14

Blockchain Registration Information

Transaction ID
e8b7115821e6e3b34bb8ee5989782b9b72a8a1cd956e107fff1b9fa5d1626735
Stamping Certificate
Download PDF (860.8 KB)
SHASUM Hash
fc869b7b8ecc15ab484283231f5d4258de62dbec608cbf9aebdd94b42b88b6bf
QR code for RSP11131

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