Cbot-2019-004

RSP 11132

Grower: Research Development Cannabinoid

General Information

Accession Date
February 5, 2019
Reported Plant Sex
not reported

The strain rarity visualization shows how distant the strain is from the other cultivars in the Kannapedia database. The y-axis represents genetic distance, getting farther as you go up. The width of the visualization at any position along the y-axis shows how many strains there are in the database at that genetic distance. So, a common strain will have a more bottom-heavy shape, while uncommon and rare cultivars will have a visualization that is generally shifted towards the top.

Rarity: Rare
Most Distant Most Similar

Chemical Information

Cannabinoid and terpenoid information provided by the grower.

Cannabinoids

No information provided.

Terpenoids

No information provided.

Genetic Information

Plant Type
Type III

The bell curve in the heterozygosity visualization shows the distribution of heterozygosity levels for cannabis cultivars in the Kannapedia database. The green line shows where this particular strain fits within the distribution. Heterozygosity is associated with heterosis (aka hybrid vigor) but also leads to the production of more variable offspring. When plants have two genetically different parents, heterozygosity levels will be higher than if it has been inbred or backcrossed repeatedly.

Heterozygosity: 0.91%
Least Heterozygous Most Heterozygous

The ratio of reads mapped to Y-contigs to reads mapped to the whole Cannabis genome (Y-ratios) has been demonstrated to be strongly correlated with plant sex typing. This plot shows the distribution of Y-ratios for all samples in our database which were sequenced with the same method (panel or WGS) as this sample and where this sample falls in the distribution.

Y-Ratio Distribution: 0.0457
male female RSP11132

This chart represents the Illumina sequence coverage over the Bt/Bd allele. These are the three regions in the cannabis genome that impact THCA, CBDA, CBGA production. Coverage over the Active CBDAS gene is highly correlated with Type II and Type III plants as described by Etienne de Meijer. Coverage over the THCA gene is highly correlated with Type I and Type II plants but is anti-correlated with Type III plants. Type I plants require coverage over the inactive CBDA loci and no coverage over the Active CBDA gene. Lack of coverage over the Active CBDA and Active THCA allele are presumed to be Type IV plants (CBGA dominant). While deletions of entire THCAS and CBDAS genes are the most common Bt:Bd alleles observed, it is possible to have plants with these genes where functional expression of the enzyme is disrupted by deactivating point mutations (Kojoma et al. 2006).

Bt/Bd Allele Coverage

This chart represents the Illumina sequence coverage over the CBCA synthase gene.

CBCAS Coverage

Variants (THCAS, CBDAS, and CBCAS)

No variants to report

Variants (Select Genes of Interest)

PKSG-2a

UniProt

c.67T>A p.Phe23Ile missense variant moderate contig700 1945567

IGV: Start, Jump

A/T
NGS:
0.825
C90:
0.904
PKSG-2a

UniProt

c.31A>T p.Thr11Ser missense variant moderate contig700 1945603

IGV: Start, Jump

T/A
NGS:
0.763
C90:
0.876
PKSG-2b

UniProt

c.1152T>A p.Asn384Lys missense variant moderate contig700 1950486

IGV: Start, Jump

A/T
NGS:
0.715
C90:
0.895
PKSG-2b

UniProt

c.1132C>G p.Leu378Val missense variant moderate contig700 1950506

IGV: Start, Jump

G/C
NGS:
0.717
C90:
0.000
PKSG-2b

UniProt

c.1117A>G p.Ile373Val missense variant moderate contig700 1950521

IGV: Start, Jump

T/C
NGS:
0.807
C90:
0.981
PKSG-2b

UniProt

c.948T>G p.Asp316Glu missense variant moderate contig700 1950690

IGV: Start, Jump

A/C
NGS:
0.456
C90:
0.000
PKSG-2b

UniProt

c.945T>G p.Ser315Arg missense variant moderate contig700 1950693

IGV: Start, Jump

A/C
NGS:
0.454
C90:
0.000
PKSG-2b

UniProt

c.944G>A p.Ser315Asn missense variant moderate contig700 1950694

IGV: Start, Jump

C/T
NGS:
0.445
C90:
0.000
PKSG-2b

UniProt

c.934C>G p.His312Asp missense variant moderate contig700 1950704

IGV: Start, Jump

G/C
NGS:
0.410
C90:
0.000
PKSG-2b

UniProt

c.31A>T p.Thr11Ser missense variant moderate contig700 1951851

IGV: Start, Jump

T/A
NGS:
0.844
C90:
0.880
PKSG-2b

UniProt

c.-2_1dupATA start lost & conservative inframe insertion high contig700 1951880

IGV: Start, Jump

A/ATAT
NGS:
0.410
C90:
0.000
PKSG-4b

UniProt

c.496A>G p.Lys166Glu missense variant moderate contig700 2721177

IGV: Start, Jump

T/C
NGS:
0.651
C90:
0.684
PKSG-4b

UniProt

c.489delT p.Phe163fs frameshift variant high contig700 2721183

IGV: Start, Jump

CA/C
NGS:
0.792
C90:
0.761
PKSG-4b

UniProt

c.485A>G p.Lys162Arg missense variant moderate contig700 2721188

IGV: Start, Jump

T/C
NGS:
0.643
C90:
0.301
PKSG-4b

UniProt

c.431T>G p.Val144Gly missense variant moderate contig700 2721242

IGV: Start, Jump

A/C
NGS:
0.575
C90:
0.679
aPT1

UniProt

c.406A>G p.Ile136Val missense variant moderate contig121 2839605

IGV: Start, Jump

A/G
NGS:
0.579
C90:
0.761
HDS-2

UniProt

c.82_93delGTAACCGGAACT p.Val28_Thr31del conservative inframe deletion moderate contig95 1989748

IGV: Start, Jump

CGTAACCGGAACT/C
NGS:
0.805
C90:
0.000
HDS-2

UniProt

c.127T>G p.Ser43Ala missense variant moderate contig95 1989794

IGV: Start, Jump

T/G
NGS:
0.721
C90:
0.000

Nearest genetic relatives (All Samples)

0 0.075 0.150 0.225 0.300
clone distance sibling distance more distant
  1. 0.155 Cbot-2019-002 (RSP11130)
  2. 0.216 Cbot-2019-003 (RSP11131)
  3. 0.228 Cbot-2019-005 (RSP11133)
  4. 0.241 Black Jack (RSP10603)
  5. 0.243 BLACK JACK (RSP11346)
  6. 0.246 RKM-2018-025 (RSP11117)
  7. 0.249 Cheese (RSP10460)
  8. 0.253 RKM-2018-016 (RSP11108)
  9. 0.255 Blueberry Cheesecake (RSP10684)
  10. 0.261 Doug s Varin (RSP11243)
  11. 0.264 Serious Happiness (RSP10763)
  12. 0.264 Cherry Blossom (RSP11315)
  13. 0.266 Durban Poison 1 (RSP10996)
  14. 0.269 Durban Poison 1 (RSP11013)
  15. 0.273 RKM-2018-027 (RSP11119)
  16. 0.273 RKM-2018-003 (RSP11094)
  17. 0.273 Liberty Haze (RSP11000)
  18. 0.274 Cherry Blossom (RSP11316)
  19. 0.274 Danny Noonan (RSP11070)
  20. 0.274 RAPHAEL 2 (RSP11172)

Nearest genetic relatives (Base Tree)

0 0.083 0.167 0.250 0.333
clone distance sibling distance more distant
  1. 0.228 Cbot-2019-005 (RSP11133)
  2. 0.255 Blueberry Cheesecake (RSP10684)
  3. 0.270 RKM-2018-027 (RSP11119)
  4. 0.273 Liberty Haze (RSP11000)
  5. 0.274 RKM-2018-006 (RSP11097)
  6. 0.275 RKM-2018-003 (RSP11094)
  7. 0.276 CST (RSP11002)
  8. 0.276 Gold Cracker (RSP11048)
  9. 0.279 Hermaphrodite ResearchSample2 (RSP11050)
  10. 0.281 Durban Poison (RSP11014)
  11. 0.284 Blueberry Cheesecake (RSP10680)
  12. 0.289 Recon (RSP10755)
  13. 0.294 QUEEN JESUS (RSP10105)
  14. 0.294 RKM-2018-009 (RSP11100)
  15. 0.300 Kush Hemp E1 (RSP11128)
  16. 0.303 Blue Dream (RSP11033)
  17. 0.306 RKM-2018-005 (RSP11096)
  18. 0.307 Golden Goat 2 (RSP10991)
  19. 0.309 RKM-2018-028 (RSP11120)
  20. 0.313 Hermaphrodite Research Sample1 (RSP11049)

Most genetically distant strains (All Samples)

0 0.117 0.233 0.350 0.467
clone distance sibling distance more distant
  1. 0.461 Cherry Blossom (RSP11328)
  2. 0.455 Cherry Blossom (RSP11333)
  3. 0.453 Cherry Blossom (RSP11317)
  4. 0.437 Cherry Blossom (RSP11330)
  5. 0.436 Cherry Blossom (RSP11318)
  6. 0.420 Red Eye OG (RSP11190)
  7. 0.413 Cherry Blossom (RSP11314)
  8. 0.407 Feral (RSP11206)
  9. 0.400 Cherry Blossom (RSP11326)
  10. 0.399 Cherry Blossom (RSP11334)
  11. 0.398 Cherry Blossom (RSP11335)
  12. 0.396 CS (RSP11208)
  13. 0.395 Cherry Blossom (RSP11309)
  14. 0.395 Cherry Blossom (RSP11324)
  15. 0.395 Cherry Blossom (RSP11310)
  16. 0.394 Cherry Blossom (RSP11322)
  17. 0.393 Cherry Blossom (RSP11308)
  18. 0.392 Cherry Blossom (RSP11311)
  19. 0.391 Cherry (RSP11142)
  20. 0.390 Cherry Blossom (RSP11323)

Most genetically distant strains (Base Tree)

0 0.100 0.200 0.300 0.400
clone distance sibling distance more distant
  1. 0.391 Cherry (RSP11142)
  2. 0.388 Skywalker OG (RSP10837)
  3. 0.374 JL yellow (RSP11075)
  4. 0.373 Carmagnola (RSP11037)
  5. 0.368 RKM-2018-022 (RSP11114)
  6. 0.363 Futura 75 (RSP10664)
  7. 0.363 Feral (RSP10890)
  8. 0.358 Ivory (RSP10668)
  9. 0.357 The Gift (RSP10988)
  10. 0.356 RKM-2018-019 (RSP11111)
  11. 0.356 Santhica27 (RSP11047)
  12. 0.353 USO 31 (RSP10981)
  13. 0.352 Tisza (RSP10659)
  14. 0.352 Monoica (RSP10241)
  15. 0.349 Fedora 17 (RSP10661)
  16. 0.349 Tisza (RSP11044)
  17. 0.349 Lovrin (RSP10658)
  18. 0.347 Carmagnola (RSP10979)
  19. 0.347 RKM-2018-026 (RSP11118)
  20. 0.344 Cherry (RSP11143)

Nearest genetic relative in Phylos dataset

Phylos Strain SRR4448321
Overlapping SNPs:
119
Concordance:
77

Nearest genetic relative in Lynch dataset

Lynch Strain SRR3495250
Overlapping SNPs:
3
Concordance:
3

Blockchain Registration Information

Transaction ID
ba26ea0c305f611928401794bb975440504b6ff385d899c7f2a9f9ec9d29ca8b
Stamping Certificate
Download PDF (859.4 KB)
SHASUM Hash
c467eb2878977bf20362d263e10fab713940486601166bc1d3b08cc8b84d9caf
QR code for RSP11132

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