Cbot-2019-006

RSP 11134

Grower: Research Development Cannabinoid

General Information

Accession Date
February 5, 2019
Reported Plant Sex
not reported

The strain rarity visualization shows how distant the strain is from the other cultivars in the Kannapedia database. The y-axis represents genetic distance, getting farther as you go up. The width of the visualization at any position along the y-axis shows how many strains there are in the database at that genetic distance. So, a common strain will have a more bottom-heavy shape, while uncommon and rare cultivars will have a visualization that is generally shifted towards the top.

Rarity: Rare
Most Distant Most Similar

Chemical Information

Cannabinoid and terpenoid information provided by the grower.

Cannabinoids

No information provided.

Terpenoids

No information provided.

Genetic Information

Plant Type
Type I

The bell curve in the heterozygosity visualization shows the distribution of heterozygosity levels for cannabis cultivars in the Kannapedia database. The green line shows where this particular strain fits within the distribution. Heterozygosity is associated with heterosis (aka hybrid vigor) but also leads to the production of more variable offspring. When plants have two genetically different parents, heterozygosity levels will be higher than if it has been inbred or backcrossed repeatedly.

Heterozygosity: 0.97%
Least Heterozygous Most Heterozygous

The ratio of reads mapped to Y-contigs to reads mapped to the whole Cannabis genome (Y-ratios) has been demonstrated to be strongly correlated with plant sex typing. This plot shows the distribution of Y-ratios for all samples in our database which were sequenced with the same method (panel or WGS) as this sample and where this sample falls in the distribution.

Y-Ratio Distribution: 0.0392
male female RSP11134

This chart represents the Illumina sequence coverage over the Bt/Bd allele. These are the three regions in the cannabis genome that impact THCA, CBDA, CBGA production. Coverage over the Active CBDAS gene is highly correlated with Type II and Type III plants as described by Etienne de Meijer. Coverage over the THCA gene is highly correlated with Type I and Type II plants but is anti-correlated with Type III plants. Type I plants require coverage over the inactive CBDA loci and no coverage over the Active CBDA gene. Lack of coverage over the Active CBDA and Active THCA allele are presumed to be Type IV plants (CBGA dominant). While deletions of entire THCAS and CBDAS genes are the most common Bt:Bd alleles observed, it is possible to have plants with these genes where functional expression of the enzyme is disrupted by deactivating point mutations (Kojoma et al. 2006).

Bt/Bd Allele Coverage

This chart represents the Illumina sequence coverage over the CBCA synthase gene.

CBCAS Coverage

Variants (THCAS, CBDAS, and CBCAS)

No variants to report

Variants (Select Genes of Interest)

PKSG-2a

UniProt

c.67T>A p.Phe23Ile missense variant moderate contig700 1945567

IGV: Start, Jump

A/T
NGS:
0.825
C90:
0.904
PKSG-2a

UniProt

c.31A>T p.Thr11Ser missense variant moderate contig700 1945603

IGV: Start, Jump

T/A
NGS:
0.763
C90:
0.876
PKSG-2b

UniProt

c.1152T>A p.Asn384Lys missense variant moderate contig700 1950486

IGV: Start, Jump

A/T
NGS:
0.715
C90:
0.895
PKSG-2b

UniProt

c.1132C>G p.Leu378Val missense variant moderate contig700 1950506

IGV: Start, Jump

G/C
NGS:
0.717
C90:
0.000
PKSG-2b

UniProt

c.1117A>G p.Ile373Val missense variant moderate contig700 1950521

IGV: Start, Jump

T/C
NGS:
0.807
C90:
0.981
PKSG-2b

UniProt

c.948T>G p.Asp316Glu missense variant moderate contig700 1950690

IGV: Start, Jump

A/C
NGS:
0.456
C90:
0.000
PKSG-2b

UniProt

c.945T>G p.Ser315Arg missense variant moderate contig700 1950693

IGV: Start, Jump

A/C
NGS:
0.454
C90:
0.000
PKSG-2b

UniProt

c.944G>A p.Ser315Asn missense variant moderate contig700 1950694

IGV: Start, Jump

C/T
NGS:
0.445
C90:
0.000
PKSG-2b

UniProt

c.934C>G p.His312Asp missense variant moderate contig700 1950704

IGV: Start, Jump

G/C
NGS:
0.410
C90:
0.000
PKSG-2b

UniProt

c.31A>T p.Thr11Ser missense variant moderate contig700 1951851

IGV: Start, Jump

T/A
NGS:
0.844
C90:
0.880
PKSG-2b

UniProt

c.-2_1dupATA start lost & conservative inframe insertion high contig700 1951880

IGV: Start, Jump

A/ATAT
NGS:
0.410
C90:
0.000
PKSG-4b

UniProt

c.496A>G p.Lys166Glu missense variant moderate contig700 2721177

IGV: Start, Jump

T/C
NGS:
0.651
C90:
0.684
PKSG-4b

UniProt

c.489delT p.Phe163fs frameshift variant high contig700 2721183

IGV: Start, Jump

CA/C
NGS:
0.792
C90:
0.761
PKSG-4b

UniProt

c.485A>G p.Lys162Arg missense variant moderate contig700 2721188

IGV: Start, Jump

T/C
NGS:
0.643
C90:
0.301
PKSG-4b

UniProt

c.431T>G p.Val144Gly missense variant moderate contig700 2721242

IGV: Start, Jump

A/C
NGS:
0.575
C90:
0.679
PKSG-4b

UniProt

c.352_355delACAG p.Thr118fs frameshift variant high contig700 2721317

IGV: Start, Jump

CCTGT/C
NGS:
0.529
C90:
0.000
aPT1

UniProt

c.629C>T p.Thr210Ile missense variant moderate contig121 2840237

IGV: Start, Jump

C/T
NGS:
0.561
C90:
0.598
HDS-2

UniProt

c.82_93delGTAACCGGAACT p.Val28_Thr31del conservative inframe deletion moderate contig95 1989748

IGV: Start, Jump

CGTAACCGGAACT/C
NGS:
0.805
C90:
0.000

Nearest genetic relatives (All Samples)

0 0.067 0.133 0.200 0.267
clone distance sibling distance more distant
  1. 0.201 Glueberry OG (RSP11222)
  2. 0.216 RKM-2018-001 (RSP11092)
  3. 0.216 Liberty Haze (RSP11000)
  4. 0.223 Domnesia (RSP11184)
  5. 0.228 Golden Goat 2 (RSP10991)
  6. 0.228 Recon (RSP10755)
  7. 0.230 Gold Cracker (RSP11041)
  8. 0.233 RKM-2018-030 (RSP11122)
  9. 0.234 Liberty Haze (RSP10946)
  10. 0.236 Erez (RSP10942)
  11. 0.237 UP All Day Jay (RSP11258)
  12. 0.237 Black Jack (RSP10603)
  13. 0.238 Serious Happiness (RSP10763)
  14. 0.238 Alaska USA (RSP11171)
  15. 0.240 UP Sunrise (RSP10989)
  16. 0.240 Gold Cracker (RSP11048)
  17. 0.242 BLACK JACK (RSP11346)
  18. 0.243 Doug s Varin (RSP11243)
  19. 0.243 RKM-2018-028 (RSP11120)
  20. 0.245 Durban Poison 1 (RSP11013)

Nearest genetic relatives (Base Tree)

0 0.075 0.150 0.225 0.300
clone distance sibling distance more distant
  1. 0.222 Liberty Haze (RSP11000)
  2. 0.225 Recon (RSP10755)
  3. 0.230 Golden Goat 2 (RSP10991)
  4. 0.242 RKM-2018-033 (RSP11125)
  5. 0.243 Blueberry Cheesecake (RSP10684)
  6. 0.244 Gold Cracker (RSP11048)
  7. 0.248 RKM-2018-028 (RSP11120)
  8. 0.253 RKM-2018-020 (RSP11112)
  9. 0.253 Italian Kiss (RSP11034)
  10. 0.255 Black Beauty (RSP11035)
  11. 0.262 Durban Poison (RSP11014)
  12. 0.262 UP Sunrise (RSP10989)
  13. 0.262 Hermaphrodite ResearchSample2 (RSP11050)
  14. 0.263 RKM-2018-029 (RSP11121)
  15. 0.264 RKM-2018-023 (RSP11115)
  16. 0.265 RKM-2018-022 (RSP11114)
  17. 0.270 RKM-2018-032 (RSP11124)
  18. 0.270 Skunk 18 (RSP11038)
  19. 0.273 RKM-2018-009 (RSP11100)
  20. 0.273 RKM-2018-018 (RSP11110)

Most genetically distant strains (All Samples)

0 0.117 0.233 0.350 0.467
clone distance sibling distance more distant
  1. 0.458 Cherry Blossom (RSP11311)
  2. 0.453 Cherry Blossom (RSP11323)
  3. 0.452 Unknown- Cherry Wine - 001 (RSP11268)
  4. 0.436 Cherry Blossom (RSP11318)
  5. 0.435 Cherry Blossom (RSP11274)
  6. 0.433 Cherry Blossom (RSP11328)
  7. 0.431 Cherry Blossom (RSP11306)
  8. 0.430 Cherry Blossom (RSP11325)
  9. 0.428 Cherry Blossom (RSP11317)
  10. 0.428 Unknown- Cherry Wine - 002 (RSP11269)
  11. 0.426 Cherry Blossom (RSP11334)
  12. 0.426 Cherry Blossom (RSP11324)
  13. 0.425 Cherry Blossom (RSP11314)
  14. 0.423 Cherry Blossom (RSP11308)
  15. 0.421 Cherry Blossom (RSP11335)
  16. 0.419 Cherry Blossom (RSP11333)
  17. 0.412 Unknown- Cherry Wine - 003 (RSP11270)
  18. 0.409 Cherry Blossom (RSP11315)
  19. 0.406 Unknown- Cherry Wine - 004 (RSP11271)
  20. 0.398 Avidekel (RSP10938)

Most genetically distant strains (Base Tree)

0 0.100 0.200 0.300 0.400
clone distance sibling distance more distant
  1. 0.375 Cbot-2019-005 (RSP11133)
  2. 0.356 Cherry (RSP11142)
  3. 0.345 Feral (RSP10890)
  4. 0.343 JL yellow (RSP11075)
  5. 0.342 Santhica27 (RSP11047)
  6. 0.341 RKM-2018-034 (RSP11126)
  7. 0.338 Ivory (RSP10668)
  8. 0.338 Carmagnola (RSP11037)
  9. 0.331 Kyrgyz Gold (RSP11054)
  10. 0.329 Monoica (RSP10241)
  11. 0.327 Skywalker OG (RSP10837)
  12. 0.326 Cherry (RSP11143)
  13. 0.325 RKM-2018-026 (RSP11118)
  14. 0.324 USO 31 (RSP10981)
  15. 0.324 Carmagnola (RSP10979)
  16. 0.320 Futura 75 (RSP10664)
  17. 0.318 Lovrin (RSP10658)
  18. 0.317 Blueberry Cheesecake (RSP10672)
  19. 0.317 Jiangji (RSP10653)
  20. 0.316 Cbot-2019-001 (RSP11129)

Nearest genetic relative in Phylos dataset

Phylos Strain SRR4451143
Overlapping SNPs:
114
Concordance:
76

Nearest genetic relative in Lynch dataset

Lynch Strain SRR3495170
Overlapping SNPs:
10
Concordance:
8

Blockchain Registration Information

Transaction ID
5535236153dd1e3c9655467bea1e08a7b07248bb47b1ce682cf139e5ddccf688
Stamping Certificate
Download PDF (858.0 KB)
SHASUM Hash
e261302f94f0a0a734b0f5b1036ca425e790a8f8a94345767281e9cb2c7b2ad8
QR code for RSP11134

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