Unknown-(Cherry Wine)-(001)

RSP 11268

Grower: Keaton Baker

General Information

Sample Name
MATTEL 001
Accession Date
September 26, 2019
Reported Plant Sex
Female
DNA Extracted From
Stem

The strain rarity visualization shows how distant the strain is from the other cultivars in the Kannapedia database. The y-axis represents genetic distance, getting farther as you go up. The width of the visualization at any position along the y-axis shows how many strains there are in the database at that genetic distance. So, a common strain will have a more bottom-heavy shape, while uncommon and rare cultivars will have a visualization that is generally shifted towards the top.

Rarity: Rare
Most Distant Most Similar

Chemical Information

Cannabinoid and terpenoid information provided by the grower.

Cannabinoids

No information provided.

Terpenoids

No information provided.

Genetic Information

Plant Type
Type III

The bell curve in the heterozygosity visualization shows the distribution of heterozygosity levels for cannabis cultivars in the Kannapedia database. The green line shows where this particular strain fits within the distribution. Heterozygosity is associated with heterosis (aka hybrid vigor) but also leads to the production of more variable offspring. When plants have two genetically different parents, heterozygosity levels will be higher than if it has been inbred or backcrossed repeatedly.

Heterozygosity: 0.93%
Least Heterozygous Most Heterozygous

The ratio of reads mapped to Y-contigs to reads mapped to the whole Cannabis genome (Y-ratios) has been demonstrated to be strongly correlated with plant sex typing. This plot shows the distribution of Y-ratios for all samples in our database which were sequenced with the same method (panel or WGS) as this sample and where this sample falls in the distribution.

Y-Ratio Distribution: 0.0424
male female RSP11268

This chart represents the Illumina sequence coverage over the Bt/Bd allele. These are the three regions in the cannabis genome that impact THCA, CBDA, CBGA production. Coverage over the Active CBDAS gene is highly correlated with Type II and Type III plants as described by Etienne de Meijer. Coverage over the THCA gene is highly correlated with Type I and Type II plants but is anti-correlated with Type III plants. Type I plants require coverage over the inactive CBDA loci and no coverage over the Active CBDA gene. Lack of coverage over the Active CBDA and Active THCA allele are presumed to be Type IV plants (CBGA dominant). While deletions of entire THCAS and CBDAS genes are the most common Bt:Bd alleles observed, it is possible to have plants with these genes where functional expression of the enzyme is disrupted by deactivating point mutations (Kojoma et al. 2006).

Bt/Bd Allele Coverage

This chart represents the Illumina sequence coverage over the CBCA synthase gene.

CBCAS Coverage

Variants (THCAS, CBDAS, and CBCAS)

CBDAS c.221C>G p.Thr74Ser missense variant moderate contig1772 2082447

IGV: Start, Jump

C/G
NGS:
0.136
C90:
0.057
CBDAS c.1420A>C p.Lys474Gln missense variant moderate contig1772 2083646

IGV: Start, Jump

A/C
NGS:
0.132
C90:
0.813

Variants (Select Genes of Interest)

PKSG-2a

UniProt

c.67T>A p.Phe23Ile missense variant moderate contig700 1945567

IGV: Start, Jump

A/T
NGS:
0.825
C90:
0.904
PKSG-2a

UniProt

c.31A>T p.Thr11Ser missense variant moderate contig700 1945603

IGV: Start, Jump

T/A
NGS:
0.763
C90:
0.876
PKSG-2b

UniProt

c.1152T>A p.Asn384Lys missense variant moderate contig700 1950486

IGV: Start, Jump

A/T
NGS:
0.715
C90:
0.895
PKSG-2b

UniProt

c.1132C>G p.Leu378Val missense variant moderate contig700 1950506

IGV: Start, Jump

G/C
NGS:
0.717
C90:
0.000
PKSG-2b

UniProt

c.1117A>G p.Ile373Val missense variant moderate contig700 1950521

IGV: Start, Jump

T/C
NGS:
0.807
C90:
0.981
PKSG-2b

UniProt

c.774G>A p.Met258Ile missense variant moderate contig700 1950864

IGV: Start, Jump

C/T
NGS:
0.366
C90:
0.947
PKSG-2b

UniProt

c.31A>T p.Thr11Ser missense variant moderate contig700 1951851

IGV: Start, Jump

T/A
NGS:
0.844
C90:
0.880
PKSG-4b

UniProt

c.496A>G p.Lys166Glu missense variant moderate contig700 2721177

IGV: Start, Jump

T/C
NGS:
0.651
C90:
0.684
PKSG-4b

UniProt

c.489delT p.Phe163fs frameshift variant high contig700 2721183

IGV: Start, Jump

CA/C
NGS:
0.792
C90:
0.761
PKSG-4b

UniProt

c.485A>G p.Lys162Arg missense variant moderate contig700 2721188

IGV: Start, Jump

T/C
NGS:
0.643
C90:
0.301
PKSG-4b

UniProt

c.431T>G p.Val144Gly missense variant moderate contig700 2721242

IGV: Start, Jump

A/C
NGS:
0.575
C90:
0.679
PKSG-4b

UniProt

c.419A>G p.Asp140Gly missense variant moderate contig700 2721254

IGV: Start, Jump

T/C
NGS:
0.430
C90:
0.440
PKSG-4b

UniProt

c.352_355delACAG p.Thr118fs frameshift variant high contig700 2721317

IGV: Start, Jump

CCTGT/C
NGS:
0.529
C90:
0.000
PKSG-4b

UniProt

c.323A>G p.Glu108Gly missense variant moderate contig700 2721350

IGV: Start, Jump

T/C
NGS:
0.469
C90:
0.000
HDS-2

UniProt

c.82_93delGTAACCGGAACT p.Val28_Thr31del conservative inframe deletion moderate contig95 1989748

IGV: Start, Jump

CGTAACCGGAACT/C
NGS:
0.805
C90:
0.000
HDS-2

UniProt

c.127T>G p.Ser43Ala missense variant moderate contig95 1989794

IGV: Start, Jump

T/G
NGS:
0.721
C90:
0.000

Nearest genetic relatives (All Samples)

0 0.067 0.133 0.200 0.267
clone distance sibling distance more distant
  1. 0.138 Cherry Blossom (RSP11321)
  2. 0.148 Unknown--Cherry Wine---002- (RSP11269)
  3. 0.189 Cherry Blossom (RSP11274)
  4. 0.204 Unknown--Cherry Wine---003- (RSP11270)
  5. 0.208 Cherry Blossom (RSP11318)
  6. 0.212 Cherry Blossom (RSP11301)
  7. 0.222 Unknown--Cherry Wine---004- (RSP11271)
  8. 0.225 Cherry Blossom (RSP11334)
  9. 0.228 Unknown--Cherry Wine---005- (RSP11272)
  10. 0.229 Cherry Blossom (RSP11311)
  11. 0.233 Cherry Blossom (RSP11328)
  12. 0.236 13 Cherries (RSP11442)
  13. 0.237 Cherry Blossom (RSP11332)
  14. 0.242 Cherry Blossom (RSP11308)
  15. 0.243 Cherry Blossom (RSP11335)
  16. 0.244 Cherry Blossom (RSP11327)
  17. 0.252 Cherry Blossom (RSP11315)
  18. 0.254 Cherry Blossom (RSP11310)
  19. 0.255 Cherry Blossom (RSP11320)
  20. 0.256 Cherry Blossom (RSP11323)

Most genetically distant strains (All Samples)

0 0.125 0.250 0.375 0.500
clone distance sibling distance more distant
  1. 0.489 GG4 (RSP11978)
  2. 0.489 Star Dawg (RSP11352)
  3. 0.487 RKM-2018-013 (RSP11104)
  4. 0.486 Abacus (RSP11266)
  5. 0.486 80E (RSP11213)
  6. 0.483 Red Eye OG (RSP11190)
  7. 0.479 80E (RSP11211)
  8. 0.475 Fatso (RSP11741)
  9. 0.475 ILM (RSP12623)
  10. 0.474 Garlic (RSP11341)
  11. 0.474 501st OG (RSP11241)
  12. 0.474 RKM-2018-032 (RSP11124)
  13. 0.473 Lemon OG Haze (RSP11977)
  14. 0.472 RKM-2018-021 (RSP11113)
  15. 0.472 RKM-2018-017 (RSP11109)
  16. 0.472 Kush Hemp E1 (RSP11128)
  17. 0.472 Rugburn OG (RSP11353)
  18. 0.472 80E (RSP11212)
  19. 0.470 GMO (RSP12091)
  20. 0.469 Skywalker OG (RSP10837)

Nearest genetic relative in Phylos dataset

Phylos Strain SRR4448204
Overlapping SNPs:
69
Concordance:
45

Nearest genetic relative in Lynch dataset

Lynch Strain SRR3495303
Overlapping SNPs:
2
Concordance:
2

Blockchain Registration Information

Transaction ID
f6c6e7ca31cb6832e6abf03efc251164dba68d164ae9b772cb914f800ab183e6
Stamping Certificate
Download PDF (859.6 KB)
SHASUM Hash
3cd68d642545e6db3dad2e5674d07730ee74ee85fd40ef3a282b3236bb728aa3
QR code for RSP11268

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