Unknown-(Cherry Wine)-(002)

RSP 11269

Grower: Keaton Baker

General Information

Sample Name
Big Bud-002
Accession Date
September 26, 2019
Reported Plant Sex
Female

The strain rarity visualization shows how distant the strain is from the other cultivars in the Kannapedia database. The y-axis represents genetic distance, getting farther as you go up. The width of the visualization at any position along the y-axis shows how many strains there are in the database at that genetic distance. So, a common strain will have a more bottom-heavy shape, while uncommon and rare cultivars will have a visualization that is generally shifted towards the top.

Rarity: Uncommon
Most Distant Most Similar

Chemical Information

Cannabinoid and terpenoid information provided by the grower.

Cannabinoids

No information provided.

Terpenoids

No information provided.

Genetic Information

Plant Type
Type III

The bell curve in the heterozygosity visualization shows the distribution of heterozygosity levels for cannabis cultivars in the Kannapedia database. The green line shows where this particular strain fits within the distribution. Heterozygosity is associated with heterosis (aka hybrid vigor) but also leads to the production of more variable offspring. When plants have two genetically different parents, heterozygosity levels will be higher than if it has been inbred or backcrossed repeatedly.

Heterozygosity: 1.04%
Least Heterozygous Most Heterozygous

The ratio of reads mapped to Y-contigs to reads mapped to the whole Cannabis genome (Y-ratios) has been demonstrated to be strongly correlated with plant sex typing. This plot shows the distribution of Y-ratios for all samples in our database which were sequenced with the same method (panel or WGS) as this sample and where this sample falls in the distribution.

Y-Ratio Distribution: 0.0406
male female RSP11269

This chart represents the Illumina sequence coverage over the Bt/Bd allele. These are the three regions in the cannabis genome that impact THCA, CBDA, CBGA production. Coverage over the Active CBDAS gene is highly correlated with Type II and Type III plants as described by Etienne de Meijer. Coverage over the THCA gene is highly correlated with Type I and Type II plants but is anti-correlated with Type III plants. Type I plants require coverage over the inactive CBDA loci and no coverage over the Active CBDA gene. Lack of coverage over the Active CBDA and Active THCA allele are presumed to be Type IV plants (CBGA dominant). While deletions of entire THCAS and CBDAS genes are the most common Bt:Bd alleles observed, it is possible to have plants with these genes where functional expression of the enzyme is disrupted by deactivating point mutations (Kojoma et al. 2006).

Bt/Bd Allele Coverage

This chart represents the Illumina sequence coverage over the CBCA synthase gene.

CBCAS Coverage

Variants (THCAS, CBDAS, and CBCAS)

CBDAS c.8G>A p.Cys3Tyr missense variant moderate contig1772 2082234

IGV: Start, Jump

G/A
NGS:
0.057
C90:
0.000
CBDAS c.221C>G p.Thr74Ser missense variant moderate contig1772 2082447

IGV: Start, Jump

C/G
NGS:
0.136
C90:
0.057
CBDAS c.1420A>C p.Lys474Gln missense variant moderate contig1772 2083646

IGV: Start, Jump

A/C
NGS:
0.132
C90:
0.813

Variants (Select Genes of Interest)

GPPs1

UniProt

c.845_848delAAAG p.Glu282fs frameshift variant high contig676 169629

IGV: Start, Jump

TGAAA/T
NGS:
0.118
C90:
0.000
PKSG-4a

UniProt

c.493G>A p.Gly165Ser missense variant moderate contig700 1937904

IGV: Start, Jump

G/A
NGS:
0.103
C90:
0.000
PKSG-4a

UniProt

c.1191_1193delTTA p.Tyr398del disruptive inframe deletion moderate contig700 1938600

IGV: Start, Jump

AATT/A
NGS:
0.167
C90:
0.000
PKSG-2a

UniProt

c.67T>A p.Phe23Ile missense variant moderate contig700 1945567

IGV: Start, Jump

A/T
NGS:
0.825
C90:
0.904
PKSG-2a

UniProt

c.31A>T p.Thr11Ser missense variant moderate contig700 1945603

IGV: Start, Jump

T/A
NGS:
0.763
C90:
0.876
PKSG-2b

UniProt

c.1152T>A p.Asn384Lys missense variant moderate contig700 1950486

IGV: Start, Jump

A/T
NGS:
0.715
C90:
0.895
PKSG-2b

UniProt

c.1132C>G p.Leu378Val missense variant moderate contig700 1950506

IGV: Start, Jump

G/C
NGS:
0.717
C90:
0.000
PKSG-2b

UniProt

c.1117A>G p.Ile373Val missense variant moderate contig700 1950521

IGV: Start, Jump

T/C
NGS:
0.807
C90:
0.981
PKSG-2b

UniProt

c.774G>A p.Met258Ile missense variant moderate contig700 1950864

IGV: Start, Jump

C/T
NGS:
0.366
C90:
0.947
PKSG-2b

UniProt

c.241G>A p.Val81Met missense variant moderate contig700 1951397

IGV: Start, Jump

C/T
NGS:
0.154
C90:
0.000
PKSG-2b

UniProt

c.240T>G p.Asp80Glu missense variant moderate contig700 1951398

IGV: Start, Jump

A/C
NGS:
0.156
C90:
0.000
PKSG-2b

UniProt

c.230T>C p.Val77Ala missense variant moderate contig700 1951408

IGV: Start, Jump

A/G
NGS:
0.134
C90:
0.646
PKSG-2b

UniProt

c.224A>G p.Lys75Arg missense variant moderate contig700 1951414

IGV: Start, Jump

T/C
NGS:
0.151
C90:
0.957
PKSG-2b

UniProt

c.31A>T p.Thr11Ser missense variant moderate contig700 1951851

IGV: Start, Jump

T/A
NGS:
0.844
C90:
0.880
PKSG-4b

UniProt

c.496A>G p.Lys166Glu missense variant moderate contig700 2721177

IGV: Start, Jump

T/C
NGS:
0.651
C90:
0.684
PKSG-4b

UniProt

c.489delT p.Phe163fs frameshift variant high contig700 2721183

IGV: Start, Jump

CA/C
NGS:
0.792
C90:
0.761
PKSG-4b

UniProt

c.485A>G p.Lys162Arg missense variant moderate contig700 2721188

IGV: Start, Jump

T/C
NGS:
0.643
C90:
0.301
PKSG-4b

UniProt

c.352_355delACAG p.Thr118fs frameshift variant high contig700 2721317

IGV: Start, Jump

CCTGT/C
NGS:
0.529
C90:
0.000
PKSG-4b

UniProt

c.324A>C p.Glu108Asp missense variant moderate contig700 2721349

IGV: Start, Jump

T/G
NGS:
0.094
C90:
0.000
PKSG-4b

UniProt

c.323A>G p.Glu108Gly missense variant moderate contig700 2721350

IGV: Start, Jump

T/C
NGS:
0.469
C90:
0.000
DXR-2

UniProt

c.1319T>C p.Ile440Thr missense variant moderate contig380 285250

IGV: Start, Jump

A/G
NGS:
0.480
C90:
0.000
HDS-2

UniProt

c.82_93delGTAACCGGAACT p.Val28_Thr31del conservative inframe deletion moderate contig95 1989748

IGV: Start, Jump

CGTAACCGGAACT/C
NGS:
0.805
C90:
0.000
HDS-2

UniProt

c.127T>G p.Ser43Ala missense variant moderate contig95 1989794

IGV: Start, Jump

T/G
NGS:
0.721
C90:
0.000

Nearest genetic relatives (All Samples)

0 0.067 0.133 0.200 0.267
clone distance sibling distance more distant
  1. 0.112 Unknown- Cherry Wine - 003 (RSP11270)
  2. 0.144 Cherry Blossom (RSP11320)
  3. 0.157 Unknown- Cherry Wine - 001 (RSP11268)
  4. 0.171 Cherry Blossom (RSP11321)
  5. 0.187 Cherry Blossom (RSP11309)
  6. 0.187 Cherry Blossom (RSP11298)
  7. 0.190 Unknown- Cherry Wine - 004 (RSP11271)
  8. 0.199 Cherry Blossom (RSP11316)
  9. 0.205 Cherry Blossom (RSP11308)
  10. 0.206 Cherry Blossom (RSP11335)
  11. 0.210 Cherry Blossom (RSP11274)
  12. 0.216 Cherry Blossom (RSP11332)
  13. 0.216 Unknown- Cherry Wine - 005 (RSP11272)
  14. 0.217 Cherry Blossom (RSP11310)
  15. 0.230 Cherry Blossom (RSP11311)
  16. 0.241 Cherry Blossom (RSP11312)
  17. 0.242 Cherry Blossom (RSP11300)
  18. 0.252 Cherry Blossom (RSP11334)
  19. 0.258 Cherry Blossom (RSP11301)
  20. 0.266 Cherry Blossom (RSP11304)

Nearest genetic relatives (Base Tree)

0 0.100 0.200 0.300 0.400
clone distance sibling distance more distant
  1. 0.312 Cherry (RSP11143)
  2. 0.326 Durban Poison (RSP11014)
  3. 0.329 UP Sunrise (RSP10989)
  4. 0.336 Blueberry Cheesecake (RSP10672)
  5. 0.338 Cherry (RSP11142)
  6. 0.340 Cbot-2019-004 (RSP11132)
  7. 0.341 Blueberry Cheesecake (RSP10684)
  8. 0.348 Liberty Haze (RSP11000)
  9. 0.354 QUEEN JESUS (RSP10105)
  10. 0.366 RKM-2018-003 (RSP11094)
  11. 0.368 CST (RSP11002)
  12. 0.386 RKM-2018-019 (RSP11111)
  13. 0.390 RKM-2018-023 (RSP11115)
  14. 0.390 Hermaphrodite ResearchSample2 (RSP11050)
  15. 0.390 Tygra (RSP10667)
  16. 0.392 Jiangji (RSP10653)
  17. 0.393 Tisza (RSP11044)
  18. 0.394 RKM-2018-028 (RSP11120)
  19. 0.395 Blueberry Cheesecake (RSP10680)
  20. 0.396 RKM-2018-005 (RSP11096)

Most genetically distant strains (All Samples)

0 0.125 0.250 0.375 0.500
clone distance sibling distance more distant
  1. 0.493 Red Eye OG (RSP11190)
  2. 0.477 RKM-2018-026 (RSP11118)
  3. 0.475 Abacus (RSP11266)
  4. 0.473 Garlic (RSP11341)
  5. 0.469 RKM-2018-013 (RSP11104)
  6. 0.469 Rugburn OG (RSP11353)
  7. 0.469 Skywalker OG (RSP10837)
  8. 0.467 SFVxTK (RSP11072)
  9. 0.459 Chem 91 (RSP11185)
  10. 0.459 501st OG (RSP11241)
  11. 0.455 80E (RSP11213)
  12. 0.453 JL yellow (RSP11075)
  13. 0.451 RKM-2018-002 (RSP11093)
  14. 0.451 Kush Hemp E1 (RSP11128)
  15. 0.450 JL 3rd Gen Mother (RSP11214)
  16. 0.450 JL 4th Gen 5 (RSP11199)
  17. 0.450 Jacks Cleaner (RSP11347)
  18. 0.449 Hermaphrodite Research Sample1 (RSP11042)
  19. 0.449 80E (RSP11211)
  20. 0.447 CS (RSP11208)

Most genetically distant strains (Base Tree)

0 0.125 0.250 0.375 0.500
clone distance sibling distance more distant
  1. 0.481 RKM-2018-026 (RSP11118)
  2. 0.471 Skywalker OG (RSP10837)
  3. 0.453 Kush Hemp E1 (RSP11128)
  4. 0.452 JL yellow (RSP11075)
  5. 0.451 RKM-2018-002 (RSP11093)
  6. 0.447 Hermaphrodite Research Sample1 (RSP11049)
  7. 0.440 RKM-2018-032 (RSP11124)
  8. 0.437 Pie Hoe (RSP11073)
  9. 0.432 RKM-2018-022 (RSP11114)
  10. 0.431 Italian Kiss (RSP11034)
  11. 0.428 Cbot-2019-006 (RSP11134)
  12. 0.428 Carmagnola (RSP11037)
  13. 0.425 RKM-2018-009 (RSP11100)
  14. 0.425 Futura 75 (RSP10664)
  15. 0.424 RKM-2018-033 (RSP11125)
  16. 0.424 Ivory (RSP10668)
  17. 0.423 RKM-2018-006 (RSP11097)
  18. 0.422 Blue Dream (RSP11033)
  19. 0.421 Lovrin (RSP10658)
  20. 0.421 Kimbo Slice (RSP10997)

Nearest genetic relative in Phylos dataset

Phylos Strain SRR8347252
Overlapping SNPs:
70
Concordance:
43

Nearest genetic relative in Lynch dataset

Lynch Strain SRR3495303
Overlapping SNPs:
2
Concordance:
2

Blockchain Registration Information

Transaction ID
2196457af1b35a8c66c5de6a537ffa8dd2403be7e75918e874f1caa5979cb0e7
Stamping Certificate
Download PDF (854.1 KB)
SHASUM Hash
e5d8698c5089492a0f4c00befc91d971c064fb8ae2a0bd46249bde284d795a4f
QR code for RSP11269

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