Cherry Blossom

RSP 11309

Grower: Yabba Cannaba

General Information

Sample Name
CK1
Accession Date
October 16, 2019
Reported Plant Sex
Female
DNA Extracted From
Stem

The strain rarity visualization shows how distant the strain is from the other cultivars in the Kannapedia database. The y-axis represents genetic distance, getting farther as you go up. The width of the visualization at any position along the y-axis shows how many strains there are in the database at that genetic distance. So, a common strain will have a more bottom-heavy shape, while uncommon and rare cultivars will have a visualization that is generally shifted towards the top.

Rarity: Rare
Most Distant Most Similar

Chemical Information

Cannabinoid and terpenoid information provided by the grower.

Cannabinoids

No information provided.

Terpenoids

No information provided.

Genetic Information

Plant Type
Type III

The bell curve in the heterozygosity visualization shows the distribution of heterozygosity levels for cannabis cultivars in the Kannapedia database. The green line shows where this particular strain fits within the distribution. Heterozygosity is associated with heterosis (aka hybrid vigor) but also leads to the production of more variable offspring. When plants have two genetically different parents, heterozygosity levels will be higher than if it has been inbred or backcrossed repeatedly.

Heterozygosity: 1.06%
Least Heterozygous Most Heterozygous

The ratio of reads mapped to Y-contigs to reads mapped to the whole Cannabis genome (Y-ratios) has been demonstrated to be strongly correlated with plant sex typing. This plot shows the distribution of Y-ratios for all samples in our database which were sequenced with the same method (panel or WGS) as this sample and where this sample falls in the distribution.

Y-Ratio Distribution: 0.0481
male female RSP11309

This chart represents the Illumina sequence coverage over the Bt/Bd allele. These are the three regions in the cannabis genome that impact THCA, CBDA, CBGA production. Coverage over the Active CBDAS gene is highly correlated with Type II and Type III plants as described by Etienne de Meijer. Coverage over the THCA gene is highly correlated with Type I and Type II plants but is anti-correlated with Type III plants. Type I plants require coverage over the inactive CBDA loci and no coverage over the Active CBDA gene. Lack of coverage over the Active CBDA and Active THCA allele are presumed to be Type IV plants (CBGA dominant). While deletions of entire THCAS and CBDAS genes are the most common Bt:Bd alleles observed, it is possible to have plants with these genes where functional expression of the enzyme is disrupted by deactivating point mutations (Kojoma et al. 2006).

Bt/Bd Allele Coverage

This chart represents the Illumina sequence coverage over the CBCA synthase gene.

CBCAS Coverage

Variants (THCAS, CBDAS, and CBCAS)

CBDAS c.221C>G p.Thr74Ser missense variant moderate contig1772 2082447

IGV: Start, Jump

C/G
NGS:
0.136
C90:
0.057
CBDAS c.1420A>C p.Lys474Gln missense variant moderate contig1772 2083646

IGV: Start, Jump

A/C
NGS:
0.132
C90:
0.813

Variants (Select Genes of Interest)

PKSG-2a

UniProt

c.67T>A p.Phe23Ile missense variant moderate contig700 1945567

IGV: Start, Jump

A/T
NGS:
0.825
C90:
0.904
PKSG-2a

UniProt

c.31A>T p.Thr11Ser missense variant moderate contig700 1945603

IGV: Start, Jump

T/A
NGS:
0.763
C90:
0.876
PKSG-2b

UniProt

c.1152T>A p.Asn384Lys missense variant moderate contig700 1950486

IGV: Start, Jump

A/T
NGS:
0.715
C90:
0.895
PKSG-2b

UniProt

c.1132C>G p.Leu378Val missense variant moderate contig700 1950506

IGV: Start, Jump

G/C
NGS:
0.717
C90:
0.000
PKSG-2b

UniProt

c.1117A>G p.Ile373Val missense variant moderate contig700 1950521

IGV: Start, Jump

T/C
NGS:
0.807
C90:
0.981
PKSG-2b

UniProt

c.774G>A p.Met258Ile missense variant moderate contig700 1950864

IGV: Start, Jump

C/T
NGS:
0.366
C90:
0.947
PKSG-2b

UniProt

c.31A>T p.Thr11Ser missense variant moderate contig700 1951851

IGV: Start, Jump

T/A
NGS:
0.844
C90:
0.880
PKSG-4b

UniProt

c.496A>G p.Lys166Glu missense variant moderate contig700 2721177

IGV: Start, Jump

T/C
NGS:
0.651
C90:
0.684
PKSG-4b

UniProt

c.489delT p.Phe163fs frameshift variant high contig700 2721183

IGV: Start, Jump

CA/C
NGS:
0.792
C90:
0.761
PKSG-4b

UniProt

c.485A>G p.Lys162Arg missense variant moderate contig700 2721188

IGV: Start, Jump

T/C
NGS:
0.643
C90:
0.301
PKSG-4b

UniProt

c.431T>G p.Val144Gly missense variant moderate contig700 2721242

IGV: Start, Jump

A/C
NGS:
0.575
C90:
0.679
PKSG-4b

UniProt

c.419A>G p.Asp140Gly missense variant moderate contig700 2721254

IGV: Start, Jump

T/C
NGS:
0.430
C90:
0.440
PKSG-4b

UniProt

c.352_355delACAG p.Thr118fs frameshift variant high contig700 2721317

IGV: Start, Jump

CCTGT/C
NGS:
0.529
C90:
0.000
PKSG-4b

UniProt

c.323A>G p.Glu108Gly missense variant moderate contig700 2721350

IGV: Start, Jump

T/C
NGS:
0.469
C90:
0.000
DXR-2

UniProt

c.1319T>C p.Ile440Thr missense variant moderate contig380 285250

IGV: Start, Jump

A/G
NGS:
0.480
C90:
0.000
HDS-2

UniProt

c.82_93delGTAACCGGAACT p.Val28_Thr31del conservative inframe deletion moderate contig95 1989748

IGV: Start, Jump

CGTAACCGGAACT/C
NGS:
0.805
C90:
0.000
HDS-2

UniProt

c.127T>G p.Ser43Ala missense variant moderate contig95 1989794

IGV: Start, Jump

T/G
NGS:
0.721
C90:
0.000

Nearest genetic relatives (All Samples)

0 0.067 0.133 0.200 0.267
clone distance sibling distance more distant
  1. 0.114 Unknown--Cherry Wine---003- (RSP11270)
  2. 0.127 Cherry Blossom (RSP11298)
  3. 0.156 Cherry Blossom (RSP11320)
  4. 0.171 Cherry Blossom (RSP11328)
  5. 0.173 Cherry Blossom (RSP11312)
  6. 0.181 Unknown--Cherry Wine---002- (RSP11269)
  7. 0.188 Unknown--Cherry Wine---005- (RSP11272)
  8. 0.190 Cherry Blossom (RSP11332)
  9. 0.210 Cherry Blossom CBG (RSP11303)
  10. 0.221 Cherry Blossom (RSP11310)
  11. 0.225 Cherry Blossom (RSP11330)
  12. 0.233 Cherry Wine (RSP11145)
  13. 0.245 Cherry Blossom (RSP11300)
  14. 0.248 Cherry Blossom (RSP11316)
  15. 0.251 Joy (RSP11380)
  16. 0.257 Cherry Blossom (RSP11317)
  17. 0.260 Cherry Blossom (RSP11301)
  18. 0.262 Cherry Blossom (RSP11313)
  19. 0.263 Cherry Blossom (RSP11304)
  20. 0.263 Electra (RSP11366)

Most genetically distant strains (All Samples)

0 0.125 0.250 0.375 0.500
clone distance sibling distance more distant
  1. 0.489 RKM-2018-022 (RSP11114)
  2. 0.482 Queen Dream CBG (RSP11287)
  3. 0.481 Kush Hemp E1 (RSP11128)
  4. 0.475 Danny Noonan (RSP11070)
  5. 0.473 Abacus (RSP11266)
  6. 0.466 Green Crack (RSP11339)
  7. 0.462 White Label 1 (RSP11336)
  8. 0.460 B52 (SRR14708255)
  9. 0.458 GMO x Garlic Breath (RSP12507)
  10. 0.458 Queen Dream CBG (RSP11282)
  11. 0.457 Tangerine Haze (RSP10995)
  12. 0.457 RKM-2018-011 (RSP11102)
  13. 0.454 RKM-2018-006 (RSP11097)
  14. 0.453 JL 3rd Gen Father (RSP11196)
  15. 0.452 Ruderalis Indica (SRR14708267)
  16. 0.452 Hindu Kush (SRR14708261)
  17. 0.450 Alpine Rocket (SRR14708266)
  18. 0.450 RKM-2018-030 (RSP11122)
  19. 0.449 Pie Scream (RSP12482)
  20. 0.449 Hermaphrodite Research Sample1 (RSP11042)

Nearest genetic relative in Phylos dataset

Phylos Strain SRR8347252
Overlapping SNPs:
66
Concordance:
39

Nearest genetic relative in Lynch dataset

Lynch Strain SRR3495257
Overlapping SNPs:
4
Concordance:
3

Blockchain Registration Information

Transaction ID
2ddb87a49cb466269d842f9e2b08a0879650f095c6df97a1472bf8ed032963ce
Stamping Certificate
Download PDF (860.2 KB)
SHASUM Hash
26cd5e46a522788647edb7f6b092c2b20e1bc07651cc0f6e4e586aaf077ca511
QR code for RSP11309

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