LEMONCAKE

RSP 11340

Grower: Happy Valley

Summary

LEMONCAKE (RSP11340/Happy Valley) is a genetic Type I plant and its genetics appear rare. The sample's closest relative in the database is RKM-2018-008 (RSP11099/R-Kiem Seeds) and it is a potential sibling. The heterozygosity rate is 1.18% which is average (65.5 percentile).

General Information

Sample Name
Lemon Cake
Accession Date
October 17, 2019
Reported Plant Sex
Female

The strain rarity visualization shows how distant the strain is from the other cultivars in the Kannapedia database. The y-axis represents genetic distance, getting farther as you go up. The width of the visualization at any position along the y-axis shows how many strains there are in the database at that genetic distance. So, a common strain will have a more bottom-heavy shape, while uncommon and rare cultivars will have a visualization that is generally shifted towards the top.

Rarity: Rare
Most Distant Most Similar

Chemical Information

Cannabinoid and terpenoid information provided by the grower.

Cannabinoids

No information provided.

Terpenoids

No information provided.

Genetic Information

Plant Type
Type I

The bell curve in the heterozygosity visualization shows the distribution of heterozygosity levels for cannabis cultivars in the Kannapedia database. The green line shows where this particular strain fits within the distribution. Heterozygosity is associated with heterosis (aka hybrid vigor) but also leads to the production of more variable offspring. When plants have two genetically different parents, heterozygosity levels will be higher than if it has been inbred or backcrossed repeatedly.

Heterozygosity: 1.18%
Least Heterozygous Most Heterozygous

The ratio of reads mapped to Y-contigs to reads mapped to the whole Cannabis genome (Y-ratios) has been demonstrated to be strongly correlated with plant sex typing. This plot shows the distribution of Y-ratios for all samples in our database which were sequenced with the same method (panel or WGS) as this sample and where this sample falls in the distribution.

Y-Ratio Distribution: 0.0481
male female RSP11340

This chart represents the Illumina sequence coverage over the Bt/Bd allele. These are the three regions in the cannabis genome that impact THCA, CBDA, CBGA production. Coverage over the Active CBDAS gene is highly correlated with Type II and Type III plants as described by Etienne de Meijer. Coverage over the THCA gene is highly correlated with Type I and Type II plants but is anti-correlated with Type III plants. Type I plants require coverage over the inactive CBDA loci and no coverage over the Active CBDA gene. Lack of coverage over the Active CBDA and Active THCA allele are presumed to be Type IV plants (CBGA dominant). While deletions of entire THCAS and CBDAS genes are the most common Bt:Bd alleles observed, it is possible to have plants with these genes where functional expression of the enzyme is disrupted by deactivating point mutations (Kojoma et al. 2006).

Bt/Bd Allele Coverage

This chart represents the Illumina sequence coverage over the CBCA synthase gene.

CBCAS Coverage

Variants (THCAS, CBDAS, and CBCAS)

Gene HGVS.c HGVS.p Annotation Annotation Impact Contig Contig Pos Ref/Alt Var Freq
THCAS c.998C>G p.Pro333Arg missense variant moderate contig741 4416830

IGV: Start, Jump

G/C
NGS:
0.182
C90:
0.000

Variants (Select Genes of Interest)

PKSG-2a

UniProt

c.67T>A p.Phe23Ile missense variant moderate contig700 1945567

IGV: Start, Jump

A/T
NGS:
0.825
C90:
0.904
PKSG-2a

UniProt

c.31A>T p.Thr11Ser missense variant moderate contig700 1945603

IGV: Start, Jump

T/A
NGS:
0.763
C90:
0.876
PKSG-2b

UniProt

c.1152T>A p.Asn384Lys missense variant moderate contig700 1950486

IGV: Start, Jump

A/T
NGS:
0.715
C90:
0.895
PKSG-2b

UniProt

c.1132C>G p.Leu378Val missense variant moderate contig700 1950506

IGV: Start, Jump

G/C
NGS:
0.717
C90:
0.000
PKSG-2b

UniProt

c.1117A>G p.Ile373Val missense variant moderate contig700 1950521

IGV: Start, Jump

T/C
NGS:
0.807
C90:
0.981
PKSG-2b

UniProt

c.995C>T p.Ser332Phe missense variant moderate contig700 1950643

IGV: Start, Jump

G/A
NGS:
0.042
C90:
0.000
PKSG-2b

UniProt

c.31A>T p.Thr11Ser missense variant moderate contig700 1951851

IGV: Start, Jump

T/A
NGS:
0.844
C90:
0.880
PKSG-4b

UniProt

c.496A>G p.Lys166Glu missense variant moderate contig700 2721177

IGV: Start, Jump

T/C
NGS:
0.651
C90:
0.684
PKSG-4b

UniProt

c.489delT p.Phe163fs frameshift variant high contig700 2721183

IGV: Start, Jump

CA/C
NGS:
0.792
C90:
0.761
PKSG-4b

UniProt

c.485A>G p.Lys162Arg missense variant moderate contig700 2721188

IGV: Start, Jump

T/C
NGS:
0.643
C90:
0.301
PKSG-4b

UniProt

c.431T>G p.Val144Gly missense variant moderate contig700 2721242

IGV: Start, Jump

A/C
NGS:
0.575
C90:
0.679
PKSG-4b

UniProt

c.419A>G p.Asp140Gly missense variant moderate contig700 2721254

IGV: Start, Jump

T/C
NGS:
0.430
C90:
0.440
PKSG-4b

UniProt

c.352_355delACAG p.Thr118fs frameshift variant high contig700 2721317

IGV: Start, Jump

CCTGT/C
NGS:
0.529
C90:
0.000
DXR-2

UniProt

c.1319T>C p.Ile440Thr missense variant moderate contig380 285250

IGV: Start, Jump

A/G
NGS:
0.480
C90:
0.000
DXR-2

UniProt

c.431C>G p.Ala144Gly missense variant moderate contig380 287760

IGV: Start, Jump

G/C
NGS:
0.182
C90:
0.550
aPT4

UniProt

c.97T>C p.Tyr33His missense variant moderate contig121 2828753

IGV: Start, Jump

T/C
NGS:
0.439
C90:
0.000
aPT4

UniProt

c.153A>C p.Lys51Asn missense variant moderate contig121 2828809

IGV: Start, Jump

A/C
NGS:
0.375
C90:
0.000
aPT4

UniProt

c.775delT p.Tyr259fs frameshift variant high contig121 2831380

IGV: Start, Jump

AT/A
NGS:
0.224
C90:
0.000
aPT4

UniProt

c.1168T>C p.Tyr390His missense variant moderate contig121 2833503

IGV: Start, Jump

T/C
NGS:
0.546
C90:
0.000
aPT1

UniProt

c.406A>G p.Ile136Val missense variant moderate contig121 2839605

IGV: Start, Jump

A/G
NGS:
0.579
C90:
0.761
aPT1

UniProt

c.629C>T p.Thr210Ile missense variant moderate contig121 2840237

IGV: Start, Jump

C/T
NGS:
0.561
C90:
0.598
HDS-2

UniProt

c.82_93delGTAACCGGAACT p.Val28_Thr31del conservative inframe deletion moderate contig95 1989748

IGV: Start, Jump

CGTAACCGGAACT/C
NGS:
0.805
C90:
0.000
HDS-2

UniProt

c.127T>G p.Ser43Ala missense variant moderate contig95 1989794

IGV: Start, Jump

T/G
NGS:
0.721
C90:
0.000

Nearest genetic relatives (All Samples)

0 0.067 0.133 0.200 0.267
clone distance sibling distance more distant
  1. 0.185 The Gift (RSP10988)
  2. 0.193 RKM-2018-008 (RSP11099)
  3. 0.225 GMO x Poison Momosa (RSP12626)
  4. 0.226 Square Wave (RSP11344)
  5. 0.229 Electra (RSP11366)
  6. 0.229 Miss X (RSP10999)
  7. 0.232 Goin Bananas (RSP12509)
  8. 0.233 Pure Power Plant (RSP11265)
  9. 0.233 Sunday Driver (RSP11071)
  10. 0.235 Doug s Varin (RSP11243)
  11. 0.237 Wedding Pie (RSP11745)
  12. 0.237 GMO x The inhaler (RSP12508)
  13. 0.238 Deadhead OG (RSP11463)
  14. 0.239 Blueberry Cheesecake (RSP10680)
  15. 0.239 Grape Stomper (RSP11180)
  16. 0.240 Black Triangle (RSP11638)
  17. 0.240 Trump x Trump (RSP11466)
  18. 0.240 Hermaphrodite ResearchSample2 (RSP11050)
  19. 0.242 SourD (RSP12092)
  20. 0.243 Pie Scream (RSP12482)

Nearest genetic relatives (Base Tree)

0 0.083 0.167 0.250 0.333
clone distance sibling distance more distant
  1. 0.208 The Gift (RSP10988)
  2. 0.240 Blueberry Cheesecake (RSP10680)
  3. 0.256 Pie Hoe (RSP11073)
  4. 0.260 Kimbo Slice (RSP10997)
  5. 0.260 Liberty Haze (RSP11000)
  6. 0.263 Kush Hemp E1 (RSP11128)
  7. 0.264 Blueberry Cheesecake (RSP10684)
  8. 0.266 Hermaphrodite ResearchSample2 (RSP11050)
  9. 0.270 RKM-2018-032 (RSP11124)
  10. 0.273 RKM-2018-026 (RSP11118)
  11. 0.281 Skunk#18 (RSP11038)
  12. 0.281 RKM-2018-019 (RSP11111)
  13. 0.284 Recon (RSP10755)
  14. 0.284 RKM-2018-022 (RSP11114)
  15. 0.285 Queen Jesus (RSP10105)
  16. 0.286 RKM-2018-034 (RSP11126)
  17. 0.293 Skywalker OG (RSP10837)
  18. 0.299 Tisza (RSP10659)
  19. 0.302 Jiangji (RSP10653)
  20. 0.308 Durban Poison (RSP11014)

Most genetically distant strains (All Samples)

0 0.117 0.233 0.350 0.467
clone distance sibling distance more distant
  1. 0.460 Cherry Blossom (RSP11323)
  2. 0.454 Tanao Sri -46- (RSP11486)
  3. 0.440 Cherry Blossom (RSP11301)
  4. 0.438 Cherry Blossom (RSP11298)
  5. 0.427 Cherry Blossom (RSP11328)
  6. 0.420 Unknown--Cherry Wine---003- (RSP11270)
  7. 0.418 Cherry Blossom (RSP11309)
  8. 0.417 JL 4th Gen 5 (RSP11199)
  9. 0.416 Chematonic -Cannatonic x Chemdawg- (RSP11394)
  10. 0.414 Cherry Blossom (RSP11322)
  11. 0.411 Unknown--Cherry Wine---001- (RSP11268)
  12. 0.410 Unknown--Cherry Wine---002- (RSP11269)
  13. 0.408 JL yellow (RSP11075)
  14. 0.406 AVIDEKEL 2 0 (RSP11174)
  15. 0.405 Cherry Blossom (RSP11318)
  16. 0.400 80E (RSP11213)
  17. 0.399 80E (RSP11211)
  18. 0.397 JL#2 (RSP11076)
  19. 0.394 Cherry Blossom (RSP11306)
  20. 0.393 JL 4th Gen 2 (RSP11194)

Most genetically distant strains (Base Tree)

0 0.100 0.200 0.300 0.400
clone distance sibling distance more distant
  1. 0.396 JL yellow (RSP11075)
  2. 0.396 Cherry (RSP11143)
  3. 0.366 RKM-2018-009 (RSP11100)
  4. 0.361 Cbot-2019-005 (RSP11133)
  5. 0.360 Monoica (RSP10241)
  6. 0.359 RKM-2018-002 (RSP11093)
  7. 0.358 Carmagnola (RSP11037)
  8. 0.357 Cherry (RSP11142)
  9. 0.356 Feral (RSP10890)
  10. 0.356 Blue Dream (RSP11033)
  11. 0.354 Hermaphrodite Research Sample1 (RSP11049)
  12. 0.353 Carmagnola (RSP10979)
  13. 0.352 RKM-2018-027 (RSP11119)
  14. 0.349 Italian Kiss (RSP11034)
  15. 0.349 Cbot-2019-001 (RSP11129)
  16. 0.348 Cbot-2019-004 (RSP11132)
  17. 0.341 RKM-2018-006 (RSP11097)
  18. 0.339 RKM-2018-031 (RSP11123)
  19. 0.339 RKM-2018-018 (RSP11110)
  20. 0.338 RKM-2018-028 (RSP11120)

Nearest genetic relative in Phylos dataset

Phylos Strain SRR8347280
Overlapping SNPs:
63
Concordance:
42

Nearest genetic relative in Lynch dataset

Lynch Strain SRR3495207
Overlapping SNPs:
6
Concordance:
6

Blockchain Registration Information

Transaction ID
8ce4b0a37dd78985f698a5a44fc291e1fd050cb6cd71312714448c00837baea1
Stamping Certificate
Download PDF (852.5 KB)
SHASUM Hash
e46b3779595ce9333b54736c764c5634dc891b80150e38466b678da4b29d1e49
QR code for RSP11340

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