CBG Berry

RSP 11446

Grower: Blue Forest Farms

General Information

Sample Name
CBG #4
Accession Date
March 31, 2020
Reported Plant Sex
Female
DNA Extracted From
Stem

The strain rarity visualization shows how distant the strain is from the other cultivars in the Kannapedia database. The y-axis represents genetic distance, getting farther as you go up. The width of the visualization at any position along the y-axis shows how many strains there are in the database at that genetic distance. So, a common strain will have a more bottom-heavy shape, while uncommon and rare cultivars will have a visualization that is generally shifted towards the top.

Rarity: Uncommon
Most Distant Most Similar

Chemical Information

Cannabinoid and terpenoid information provided by the grower.

Cannabinoids

No information provided.

Terpenoids

No information provided.

Genetic Information

Plant Type
Type I

The bell curve in the heterozygosity visualization shows the distribution of heterozygosity levels for cannabis cultivars in the Kannapedia database. The green line shows where this particular strain fits within the distribution. Heterozygosity is associated with heterosis (aka hybrid vigor) but also leads to the production of more variable offspring. When plants have two genetically different parents, heterozygosity levels will be higher than if it has been inbred or backcrossed repeatedly.

Heterozygosity: 1.09%
Least Heterozygous Most Heterozygous

The ratio of reads mapped to Y-contigs to reads mapped to the whole Cannabis genome (Y-ratios) has been demonstrated to be strongly correlated with plant sex typing. This plot shows the distribution of Y-ratios for all samples in our database which were sequenced with the same method (panel or WGS) as this sample and where this sample falls in the distribution.

Y-Ratio Distribution: 0.0502
male female RSP11446

This chart represents the Illumina sequence coverage over the Bt/Bd allele. These are the three regions in the cannabis genome that impact THCA, CBDA, CBGA production. Coverage over the Active CBDAS gene is highly correlated with Type II and Type III plants as described by Etienne de Meijer. Coverage over the THCA gene is highly correlated with Type I and Type II plants but is anti-correlated with Type III plants. Type I plants require coverage over the inactive CBDA loci and no coverage over the Active CBDA gene. Lack of coverage over the Active CBDA and Active THCA allele are presumed to be Type IV plants (CBGA dominant). While deletions of entire THCAS and CBDAS genes are the most common Bt:Bd alleles observed, it is possible to have plants with these genes where functional expression of the enzyme is disrupted by deactivating point mutations (Kojoma et al. 2006).

Bt/Bd Allele Coverage

This chart represents the Illumina sequence coverage over the CBCA synthase gene.

CBCAS Coverage

Variants (THCAS, CBDAS, and CBCAS)

THCAS c.1064G>A p.Ser355Asn missense variant moderate contig741 4416764

IGV: Start, Jump

C/T
NGS:
0.018
C90:
0.000
THCAS c.998C>G p.Pro333Arg missense variant moderate contig741 4416830

IGV: Start, Jump

G/C
NGS:
0.182
C90:
0.000

Variants (Select Genes of Interest)

GPPs1

UniProt

c.845_848delAAAG p.Glu282fs frameshift variant high contig676 169629

IGV: Start, Jump

TGAAA/T
NGS:
0.118
C90:
0.000
PKSG-2a

UniProt

c.67T>A p.Phe23Ile missense variant moderate contig700 1945567

IGV: Start, Jump

A/T
NGS:
0.825
C90:
0.904
PKSG-2a

UniProt

c.31A>T p.Thr11Ser missense variant moderate contig700 1945603

IGV: Start, Jump

T/A
NGS:
0.763
C90:
0.876
PKSG-2b

UniProt

c.1152T>A p.Asn384Lys missense variant moderate contig700 1950486

IGV: Start, Jump

A/T
NGS:
0.715
C90:
0.895
PKSG-2b

UniProt

c.1132C>G p.Leu378Val missense variant moderate contig700 1950506

IGV: Start, Jump

G/C
NGS:
0.717
C90:
0.000
PKSG-2b

UniProt

c.1117A>G p.Ile373Val missense variant moderate contig700 1950521

IGV: Start, Jump

T/C
NGS:
0.807
C90:
0.981
PKSG-2b

UniProt

c.948T>G p.Asp316Glu missense variant moderate contig700 1950690

IGV: Start, Jump

A/C
NGS:
0.456
C90:
0.000
PKSG-2b

UniProt

c.945T>G p.Ser315Arg missense variant moderate contig700 1950693

IGV: Start, Jump

A/C
NGS:
0.454
C90:
0.000
PKSG-2b

UniProt

c.944G>A p.Ser315Asn missense variant moderate contig700 1950694

IGV: Start, Jump

C/T
NGS:
0.445
C90:
0.000
PKSG-2b

UniProt

c.934C>G p.His312Asp missense variant moderate contig700 1950704

IGV: Start, Jump

G/C
NGS:
0.410
C90:
0.000
PKSG-2b

UniProt

c.31A>T p.Thr11Ser missense variant moderate contig700 1951851

IGV: Start, Jump

T/A
NGS:
0.844
C90:
0.880
PKSG-2b

UniProt

c.-2_1dupATA start lost & conservative inframe insertion high contig700 1951880

IGV: Start, Jump

A/ATAT
NGS:
0.410
C90:
0.000
DXR-2

UniProt

c.1319T>C p.Ile440Thr missense variant moderate contig380 285250

IGV: Start, Jump

A/G
NGS:
0.480
C90:
0.000
aPT4

UniProt

c.97T>C p.Tyr33His missense variant moderate contig121 2828753

IGV: Start, Jump

T/C
NGS:
0.439
C90:
0.000
aPT4

UniProt

c.153A>C p.Lys51Asn missense variant moderate contig121 2828809

IGV: Start, Jump

A/C
NGS:
0.375
C90:
0.000
aPT4

UniProt

c.235_236delGT p.Val79fs frameshift variant high contig121 2829030

IGV: Start, Jump

ATG/A
NGS:
0.417
C90:
0.000
aPT4

UniProt

c.238delT p.Ser80fs frameshift variant high contig121 2829034

IGV: Start, Jump

AT/A
NGS:
0.421
C90:
0.000
aPT4

UniProt

c.302A>G p.Asn101Ser missense variant moderate contig121 2829099

IGV: Start, Jump

A/G
NGS:
0.349
C90:
0.000
aPT4

UniProt

c.1168T>C p.Tyr390His missense variant moderate contig121 2833503

IGV: Start, Jump

T/C
NGS:
0.546
C90:
0.000
aPT1

UniProt

c.629C>T p.Thr210Ile missense variant moderate contig121 2840237

IGV: Start, Jump

C/T
NGS:
0.561
C90:
0.598
HDS-2

UniProt

c.82_93delGTAACCGGAACT p.Val28_Thr31del conservative inframe deletion moderate contig95 1989748

IGV: Start, Jump

CGTAACCGGAACT/C
NGS:
0.805
C90:
0.000
HDS-2

UniProt

c.127T>G p.Ser43Ala missense variant moderate contig95 1989794

IGV: Start, Jump

T/G
NGS:
0.721
C90:
0.000

Nearest genetic relatives (All Samples)

0 0.058 0.117 0.175 0.233
closely related moderately related distantly related
  1. 0.110 Doug s Varin (RSP11243)
  2. 0.141 CBG-#40 (RSP11444)
  3. 0.145 Electra (RSP11366)
  4. 0.154 CBG #30 (RSP11447)
  5. 0.156 Lift (RSP11378)
  6. 0.165 CBG Berry (RSP11445)
  7. 0.171 Joy (RSP11380)
  8. 0.174 UnObtanium (RSP11611)
  9. 0.186 Durban Poison #1 (RSP11013)
  10. 0.194 Suver Haze (RSP11364)
  11. 0.196 Serious Happiness (RSP10763)
  12. 0.200 Trump x Trump (RSP11466)
  13. 0.203 Blueberry Cheesecake (RSP10684)
  14. 0.206 Durban Poison #1 (RSP10996)
  15. 0.208 Lifter (RSP11365)
  16. 0.210 Liberty Haze (RSP11000)
  17. 0.211 Rest (RSP11377)
  18. 0.214 JL X NSPM1 14 (RSP11473)
  19. 0.214 Domnesia (RSP11184)
  20. 0.214 CBG Fiber (RSP11440)

Most genetically distant strains (All Samples)

0 0.117 0.233 0.350 0.467
closely related moderately related distantly related
  1. 0.446 80E (RSP11213)
  2. 0.437 Cherry Blossom (RSP11323)
  3. 0.424 80E (RSP11211)
  4. 0.423 80E (RSP11212)
  5. 0.420 R1in136 (SRR14708226)
  6. 0.414 Feral (RSP11206)
  7. 0.407 Cherry Blossom (RSP11306)
  8. 0.404 CS (RSP11208)
  9. 0.404 Feral (RSP11205)
  10. 0.402 Chem 91 (RSP11185)
  11. 0.401 VIR 223 - Bernburgskaya Odnodomnaya - bm (SRR14708217)
  12. 0.398 VIR 483 (SRR14708239)
  13. 0.397 R1in136 (SRR14708237)
  14. 0.397 GMO x Garlic Breath (RSP12507)
  15. 0.396 Delta-llosa (SRR14708272)
  16. 0.395 Juso14 (SRR14708259)
  17. 0.395 Feral (RSP10890)
  18. 0.394 Chematonic -Cannatonic x Chemdawg- (RSP11394)
  19. 0.394 Feral (RSP10892)
  20. 0.394 Carmagnola (RSP11037)

Nearest genetic relative in Phylos dataset

Phylos Strain SRR4451151
Overlapping SNPs:
81
Concordance:
48

Nearest genetic relative in Lynch dataset

Lynch Strain SRR3495180
Overlapping SNPs:
6
Concordance:
6

Blockchain Registration Information

Transaction ID
9aff1861bdd1afc98890204fce9563e45a434667b1870b6c9323cb61db18d4e8
Stamping Certificate
Download PDF (39.9 KB)
SHASUM Hash
0f99fa84bec6bb974e892b34c21906f9cf20483bfd815d86e945c562b97d2fd2
QR code for RSP11446

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