NSPM_x_NSPM

RSP 11487

Grower: Kevin Mckernan

General Information

Sample Name
Tent_Outdoor
Accession Date
May 21, 2020
Reported Plant Sex
Female

The strain rarity visualization shows how distant the strain is from the other cultivars in the Kannapedia database. The y-axis represents genetic distance, getting farther as you go up. The width of the visualization at any position along the y-axis shows how many strains there are in the database at that genetic distance. So, a common strain will have a more bottom-heavy shape, while uncommon and rare cultivars will have a visualization that is generally shifted towards the top.

Rarity: Uncommon
Most Distant Most Similar

Chemical Information

Cannabinoid and terpenoid information provided by the grower.

Cannabinoids

No information provided.

Terpenoids

No information provided.

Genetic Information

Plant Type
Type I

The bell curve in the heterozygosity visualization shows the distribution of heterozygosity levels for cannabis cultivars in the Kannapedia database. The green line shows where this particular strain fits within the distribution. Heterozygosity is associated with heterosis (aka hybrid vigor) but also leads to the production of more variable offspring. When plants have two genetically different parents, heterozygosity levels will be higher than if it has been inbred or backcrossed repeatedly.

Heterozygosity: 1.08%
Least Heterozygous Most Heterozygous

The ratio of reads mapped to Y-contigs to reads mapped to the whole Cannabis genome (Y-ratios) has been demonstrated to be strongly correlated with plant sex typing. This plot shows the distribution of Y-ratios for all samples in our database which were sequenced with the same method (panel or WGS) as this sample and where this sample falls in the distribution.

Y-Ratio Distribution: 0.0401
male female RSP11487

This chart represents the Illumina sequence coverage over the Bt/Bd allele. These are the three regions in the cannabis genome that impact THCA, CBDA, CBGA production. Coverage over the Active CBDAS gene is highly correlated with Type II and Type III plants as described by Etienne de Meijer. Coverage over the THCA gene is highly correlated with Type I and Type II plants but is anti-correlated with Type III plants. Type I plants require coverage over the inactive CBDA loci and no coverage over the Active CBDA gene. Lack of coverage over the Active CBDA and Active THCA allele are presumed to be Type IV plants (CBGA dominant). While deletions of entire THCAS and CBDAS genes are the most common Bt:Bd alleles observed, it is possible to have plants with these genes where functional expression of the enzyme is disrupted by deactivating point mutations (Kojoma et al. 2006).

Bt/Bd Allele Coverage

This chart represents the Illumina sequence coverage over the CBCA synthase gene.

CBCAS Coverage

Variants (THCAS, CBDAS, and CBCAS)

Gene HGVS.c HGVS.p Annotation Annotation Impact Contig Contig Pos Ref/Alt Var Freq
THCAS c.998C>G p.Pro333Arg missense variant moderate contig741 4416830

IGV: Start, Jump

G/C
NGS:
0.182
C90:
0.000

Variants (Select Genes of Interest)

PKSG-2a

UniProt

c.781T>A p.Leu261Ile missense variant moderate contig700 1944609

IGV: Start, Jump

A/T
NGS:
0.026
C90:
0.574
PKSG-2a

UniProt

c.774G>A p.Met258Ile missense variant moderate contig700 1944616

IGV: Start, Jump

C/T
NGS:
0.123
C90:
0.000
PKSG-2a

UniProt

c.241G>A p.Val81Met missense variant moderate contig700 1945149

IGV: Start, Jump

C/T
NGS:
0.136
C90:
0.000
PKSG-2a

UniProt

c.240T>G p.Asp80Glu missense variant moderate contig700 1945150

IGV: Start, Jump

A/C
NGS:
0.136
C90:
0.000
PKSG-2a

UniProt

c.230T>C p.Val77Ala missense variant moderate contig700 1945160

IGV: Start, Jump

A/G
NGS:
0.114
C90:
0.000
PKSG-2a

UniProt

c.224A>G p.Lys75Arg missense variant moderate contig700 1945166

IGV: Start, Jump

T/C
NGS:
0.143
C90:
0.962
PKSG-2a

UniProt

c.188T>G p.Ile63Ser missense variant moderate contig700 1945202

IGV: Start, Jump

A/C
NGS:
0.101
C90:
0.000
PKSG-2a

UniProt

c.188T>A p.Ile63Asn missense variant moderate contig700 1945202

IGV: Start, Jump

A/T
NGS:
0.061
C90:
0.000
PKSG-2a

UniProt

c.187A>T p.Ile63Phe missense variant moderate contig700 1945203

IGV: Start, Jump

T/A
NGS:
0.101
C90:
0.000
PKSG-2a

UniProt

c.167C>G p.Thr56Ser missense variant moderate contig700 1945223

IGV: Start, Jump

G/C
NGS:
0.075
C90:
0.000
PKSG-2a

UniProt

c.67T>A p.Phe23Ile missense variant moderate contig700 1945567

IGV: Start, Jump

A/T
NGS:
0.825
C90:
0.904
PKSG-2a

UniProt

c.31A>T p.Thr11Ser missense variant moderate contig700 1945603

IGV: Start, Jump

T/A
NGS:
0.763
C90:
0.876
PKSG-2b

UniProt

c.1152T>A p.Asn384Lys missense variant moderate contig700 1950486

IGV: Start, Jump

A/T
NGS:
0.715
C90:
0.895
PKSG-2b

UniProt

c.1132C>G p.Leu378Val missense variant moderate contig700 1950506

IGV: Start, Jump

G/C
NGS:
0.717
C90:
0.000
PKSG-2b

UniProt

c.1117A>G p.Ile373Val missense variant moderate contig700 1950521

IGV: Start, Jump

T/C
NGS:
0.807
C90:
0.981
PKSG-2b

UniProt

c.948T>G p.Asp316Glu missense variant moderate contig700 1950690

IGV: Start, Jump

A/C
NGS:
0.456
C90:
0.000
PKSG-2b

UniProt

c.945T>G p.Ser315Arg missense variant moderate contig700 1950693

IGV: Start, Jump

A/C
NGS:
0.454
C90:
0.000
PKSG-2b

UniProt

c.944G>A p.Ser315Asn missense variant moderate contig700 1950694

IGV: Start, Jump

C/T
NGS:
0.445
C90:
0.000
PKSG-2b

UniProt

c.934C>G p.His312Asp missense variant moderate contig700 1950704

IGV: Start, Jump

G/C
NGS:
0.410
C90:
0.000
PKSG-2b

UniProt

c.774G>A p.Met258Ile missense variant moderate contig700 1950864

IGV: Start, Jump

C/T
NGS:
0.366
C90:
0.947
PKSG-2b

UniProt

c.718T>A p.Phe240Ile missense variant moderate contig700 1950920

IGV: Start, Jump

A/T
NGS:
0.114
C90:
0.000
PKSG-2b

UniProt

c.241G>A p.Val81Met missense variant moderate contig700 1951397

IGV: Start, Jump

C/T
NGS:
0.154
C90:
0.000
PKSG-2b

UniProt

c.240T>G p.Asp80Glu missense variant moderate contig700 1951398

IGV: Start, Jump

A/C
NGS:
0.156
C90:
0.000
PKSG-2b

UniProt

c.230T>C p.Val77Ala missense variant moderate contig700 1951408

IGV: Start, Jump

A/G
NGS:
0.134
C90:
0.646
PKSG-2b

UniProt

c.224A>G p.Lys75Arg missense variant moderate contig700 1951414

IGV: Start, Jump

T/C
NGS:
0.151
C90:
0.957
PKSG-2b

UniProt

c.188T>G p.Ile63Ser missense variant moderate contig700 1951450

IGV: Start, Jump

A/C
NGS:
0.118
C90:
0.000
PKSG-2b

UniProt

c.187A>T p.Ile63Phe missense variant moderate contig700 1951451

IGV: Start, Jump

T/A
NGS:
0.116
C90:
0.000
PKSG-2b

UniProt

c.167C>G p.Thr56Ser missense variant moderate contig700 1951471

IGV: Start, Jump

G/C
NGS:
0.103
C90:
0.947
PKSG-2b

UniProt

c.31A>T p.Thr11Ser missense variant moderate contig700 1951851

IGV: Start, Jump

T/A
NGS:
0.844
C90:
0.880
PKSG-2b

UniProt

c.-2_1dupATA start lost & conservative inframe insertion high contig700 1951880

IGV: Start, Jump

A/ATAT
NGS:
0.410
C90:
0.000
PKSG-4b

UniProt

c.527G>T p.Trp176Leu missense variant moderate contig700 2721146

IGV: Start, Jump

C/A
NGS:
0.033
C90:
0.000
PKSG-4b

UniProt

c.526T>C p.Trp176Arg missense variant moderate contig700 2721147

IGV: Start, Jump

A/G
NGS:
0.037
C90:
0.000
PKSG-4b

UniProt

c.496A>G p.Lys166Glu missense variant moderate contig700 2721177

IGV: Start, Jump

T/C
NGS:
0.651
C90:
0.684
PKSG-4b

UniProt

c.489delT p.Phe163fs frameshift variant high contig700 2721183

IGV: Start, Jump

CA/C
NGS:
0.792
C90:
0.761
PKSG-4b

UniProt

c.485A>G p.Lys162Arg missense variant moderate contig700 2721188

IGV: Start, Jump

T/C
NGS:
0.643
C90:
0.301
PKSG-4b

UniProt

c.431T>G p.Val144Gly missense variant moderate contig700 2721242

IGV: Start, Jump

A/C
NGS:
0.575
C90:
0.679
PKSG-4b

UniProt

c.419A>G p.Asp140Gly missense variant moderate contig700 2721254

IGV: Start, Jump

T/C
NGS:
0.430
C90:
0.440
PKSG-4b

UniProt

c.352_355delACAG p.Thr118fs frameshift variant high contig700 2721317

IGV: Start, Jump

CCTGT/C
NGS:
0.529
C90:
0.000
PKSG-4b

UniProt

c.323A>G p.Glu108Gly missense variant moderate contig700 2721350

IGV: Start, Jump

T/C
NGS:
0.469
C90:
0.000
DXR-2

UniProt

c.1319T>C p.Ile440Thr missense variant moderate contig380 285250

IGV: Start, Jump

A/G
NGS:
0.480
C90:
0.000
DXR-2

UniProt

c.431C>G p.Ala144Gly missense variant moderate contig380 287760

IGV: Start, Jump

G/C
NGS:
0.182
C90:
0.550
aPT4

UniProt

c.16G>A p.Val6Ile missense variant moderate contig121 2828672

IGV: Start, Jump

G/A
NGS:
0.158
C90:
0.000
aPT4

UniProt

c.97T>C p.Tyr33His missense variant moderate contig121 2828753

IGV: Start, Jump

T/C
NGS:
0.439
C90:
0.000
aPT4

UniProt

c.153A>C p.Lys51Asn missense variant moderate contig121 2828809

IGV: Start, Jump

A/C
NGS:
0.375
C90:
0.000
aPT4

UniProt

c.235_236delGT p.Val79fs frameshift variant high contig121 2829030

IGV: Start, Jump

ATG/A
NGS:
0.417
C90:
0.000
aPT4

UniProt

c.238delT p.Ser80fs frameshift variant high contig121 2829034

IGV: Start, Jump

AT/A
NGS:
0.421
C90:
0.000
aPT4

UniProt

c.302A>G p.Asn101Ser missense variant moderate contig121 2829099

IGV: Start, Jump

A/G
NGS:
0.349
C90:
0.000
aPT4

UniProt

c.775delT p.Tyr259fs frameshift variant high contig121 2831380

IGV: Start, Jump

AT/A
NGS:
0.224
C90:
0.000
aPT4

UniProt

c.1168T>C p.Tyr390His missense variant moderate contig121 2833503

IGV: Start, Jump

T/C
NGS:
0.546
C90:
0.000
aPT1

UniProt

c.406A>G p.Ile136Val missense variant moderate contig121 2839605

IGV: Start, Jump

A/G
NGS:
0.579
C90:
0.761
aPT1

UniProt

c.629C>T p.Thr210Ile missense variant moderate contig121 2840237

IGV: Start, Jump

C/T
NGS:
0.561
C90:
0.598
HDS-2

UniProt

c.82_93delGTAACCGGAACT p.Val28_Thr31del conservative inframe deletion moderate contig95 1989748

IGV: Start, Jump

CGTAACCGGAACT/C
NGS:
0.805
C90:
0.000
HDS-2

UniProt

c.127T>G p.Ser43Ala missense variant moderate contig95 1989794

IGV: Start, Jump

T/G
NGS:
0.721
C90:
0.000
HDS-1

UniProt

c.1378G>A p.Val460Ile missense variant moderate contig1891 886370

IGV: Start, Jump

C/T
NGS:
0.167
C90:
0.000
HDS-1

UniProt

c.-108+1_-108+2insG splice donor variant & intron variant high contig1891 889975

IGV: Start, Jump

A/AC
NGS:
0.217
C90:
0.000

Nearest genetic relatives (All Samples)

0 0.058 0.117 0.175 0.233
clone distance sibling distance more distant
  1. 0.162 NSPM1 (RSP11362)
  2. 0.171 Garlic (RSP11341)
  3. 0.177 Domnesia (RSP11184)
  4. 0.192 Gorilla Cookies (RSP11231)
  5. 0.194 SHERBERT (RSP11355)
  6. 0.198 JL Tent 2 (RSP11489)
  7. 0.202 JL X NSPM1 14 (RSP11473)
  8. 0.205 Serious Happiness (RSP10763)
  9. 0.206 JL Tent 3 (RSP11490)
  10. 0.213 Casco Kush (RSP11167)
  11. 0.213 JL X NSPM1 22 (RSP11475)
  12. 0.213 JL x NSPM1 1 (RSP11478)
  13. 0.214 Star Dawg (RSP11352)
  14. 0.215 JL Tent 1 yellow stake (RSP11488)
  15. 0.216 RKM-2018-034 (RSP11126)
  16. 0.217 Super Blue Dream (RSP11011)
  17. 0.218 Electra (RSP11366)
  18. 0.218 SFVxTK (RSP11072)
  19. 0.219 Blue Dream (RSP11017)
  20. 0.220 Dominion Skunk (RSP11354)

Nearest genetic relatives (Base Tree)

0 0.075 0.150 0.225 0.300
clone distance sibling distance more distant
  1. 0.216 RKM-2018-034 (RSP11126)
  2. 0.225 Skywalker OG (RSP10837)
  3. 0.235 Blueberry Cheesecake (RSP10684)
  4. 0.242 Hermaphrodite ResearchSample2 (RSP11050)
  5. 0.249 The Gift (RSP10988)
  6. 0.253 Pie Hoe (RSP11073)
  7. 0.255 RKM-2018-026 (RSP11118)
  8. 0.261 RKM-2018-032 (RSP11124)
  9. 0.268 RKM-2018-033 (RSP11125)
  10. 0.268 RKM-2018-009 (RSP11100)
  11. 0.277 Gold Cracker (RSP11048)
  12. 0.277 Durban Poison (RSP11014)
  13. 0.278 Liberty Haze (RSP11000)
  14. 0.280 Recon (RSP10755)
  15. 0.282 Hermaphrodite Research Sample1 (RSP11049)
  16. 0.286 Golden Goat 2 (RSP10991)
  17. 0.287 RKM-2018-005 (RSP11096)
  18. 0.287 Blueberry Cheesecake (RSP10680)
  19. 0.289 Blue Dream (RSP11033)
  20. 0.290 RKM-2018-020 (RSP11112)

Most genetically distant strains (All Samples)

0 0.125 0.250 0.375 0.500
clone distance sibling distance more distant
  1. 0.484 Cherry Blossom (RSP11323)
  2. 0.475 Cherry Blossom (RSP11318)
  3. 0.456 Tanao Sri 46 (RSP11486)
  4. 0.441 Unknown- Cherry Wine - 001 (RSP11268)
  5. 0.441 AVIDEKEL 2 0 (RSP11174)
  6. 0.440 Cherry Blossom (RSP11328)
  7. 0.435 Cherry Blossom (RSP11325)
  8. 0.433 Cherry Blossom (RSP11306)
  9. 0.419 Cherry Blossom (RSP11301)
  10. 0.415 Brunswick High (RSP11164)
  11. 0.413 Chematonic Cannatonic x Chemdawg (RSP11394)
  12. 0.411 Unknown- Cherry Wine - 003 (RSP11270)
  13. 0.411 80E (RSP11213)
  14. 0.410 Cherry Blossom (RSP11274)
  15. 0.410 Unknown- Cherry Wine - 002 (RSP11269)
  16. 0.410 Cherry Blossom (RSP11312)
  17. 0.410 JL yellow (RSP11075)
  18. 0.408 Cherry Blossom (RSP11300)
  19. 0.407 Cherry Blossom (RSP11309)
  20. 0.405 JL 2 (RSP11076)

Most genetically distant strains (Base Tree)

0 0.108 0.217 0.325 0.433
clone distance sibling distance more distant
  1. 0.426 JL yellow (RSP11075)
  2. 0.400 Carmagnola (RSP11037)
  3. 0.389 Carmagnola (RSP10979)
  4. 0.388 Cbot-2019-001 (RSP11129)
  5. 0.387 Santhica27 (RSP11047)
  6. 0.384 Cherry (RSP11143)
  7. 0.384 Monoica (RSP10241)
  8. 0.379 Futura 75 (RSP10664)
  9. 0.378 Feral (RSP10890)
  10. 0.377 Cbot-2019-005 (RSP11133)
  11. 0.375 RKM-2018-018 (RSP11110)
  12. 0.374 Ivory (RSP10668)
  13. 0.374 Blueberry Cheesecake (RSP10672)
  14. 0.373 RKM-2018-028 (RSP11120)
  15. 0.368 Tisza (RSP11044)
  16. 0.367 USO 31 (RSP10981)
  17. 0.367 Fedora 17 (RSP10661)
  18. 0.365 Lovrin (RSP10658)
  19. 0.363 Black Beauty (RSP11035)
  20. 0.359 Cbot-2019-004 (RSP11132)

Nearest genetic relative in Phylos dataset

Phylos Strain SRR8347212
Overlapping SNPs:
83
Concordance:
54

Nearest genetic relative in Lynch dataset

Lynch Strain SRR3495196
Overlapping SNPs:
2
Concordance:
2

Blockchain Registration Information

Transaction ID
21852e3155c8d4b76540f316512fc41b5d427bda0d31c546336a2eec002cfd6f
Stamping Certificate
Download PDF (39.6 KB)
SHASUM Hash
0536735a84c975890c90f520586e62d4be73d426a5891a9435dbdbbd53e703e8
QR code for RSP11487

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