Black Triangle

RSP 11638

Grower: Happy Valley

Summary

Black Triangle (RSP11638/Happy Valley) is a genetic Type I plant and its genetics appear uncommon. The sample's closest relative in the database is RKM-2018-008 (RSP11099/R-Kiem Seeds) and it is a potential sibling. The heterozygosity rate is 1.13% which is average (58.2 percentile).

General Information

Accession Date
September 17, 2020
Reported Plant Sex
Female

The strain rarity visualization shows how distant the strain is from the other cultivars in the Kannapedia database. The y-axis represents genetic distance, getting farther as you go up. The width of the visualization at any position along the y-axis shows how many strains there are in the database at that genetic distance. So, a common strain will have a more bottom-heavy shape, while uncommon and rare cultivars will have a visualization that is generally shifted towards the top.

Rarity: Uncommon
Most Distant Most Similar

Chemical Information

Cannabinoid and terpenoid information provided by the grower.

Cannabinoids

No information provided.

Terpenoids

No information provided.

Genetic Information

Plant Type
Type I

The bell curve in the heterozygosity visualization shows the distribution of heterozygosity levels for cannabis cultivars in the Kannapedia database. The green line shows where this particular strain fits within the distribution. Heterozygosity is associated with heterosis (aka hybrid vigor) but also leads to the production of more variable offspring. When plants have two genetically different parents, heterozygosity levels will be higher than if it has been inbred or backcrossed repeatedly.

Heterozygosity: 1.13%
Least Heterozygous Most Heterozygous

The ratio of reads mapped to Y-contigs to reads mapped to the whole Cannabis genome (Y-ratios) has been demonstrated to be strongly correlated with plant sex typing. This plot shows the distribution of Y-ratios for all samples in our database which were sequenced with the same method (panel or WGS) as this sample and where this sample falls in the distribution.

Y-Ratio Distribution: 0.0433
male female RSP11638

This chart represents the Illumina sequence coverage over the Bt/Bd allele. These are the three regions in the cannabis genome that impact THCA, CBDA, CBGA production. Coverage over the Active CBDAS gene is highly correlated with Type II and Type III plants as described by Etienne de Meijer. Coverage over the THCA gene is highly correlated with Type I and Type II plants but is anti-correlated with Type III plants. Type I plants require coverage over the inactive CBDA loci and no coverage over the Active CBDA gene. Lack of coverage over the Active CBDA and Active THCA allele are presumed to be Type IV plants (CBGA dominant). While deletions of entire THCAS and CBDAS genes are the most common Bt:Bd alleles observed, it is possible to have plants with these genes where functional expression of the enzyme is disrupted by deactivating point mutations (Kojoma et al. 2006).

Bt/Bd Allele Coverage

This chart represents the Illumina sequence coverage over the CBCA synthase gene.

CBCAS Coverage

Variants (THCAS, CBDAS, and CBCAS)

THCAS c.998C>G p.Pro333Arg missense variant moderate contig741 4416830

IGV: Start, Jump

G/C
NGS:
0.182
C90:
0.000
THCAS c.749C>A p.Ala250Asp missense variant moderate contig741 4417079

IGV: Start, Jump

G/T
NGS:
0.127
C90:
0.632

Variants (Select Genes of Interest)

PKSG-2a

UniProt

c.67T>A p.Phe23Ile missense variant moderate contig700 1945567

IGV: Start, Jump

A/T
NGS:
0.825
C90:
0.904
PKSG-2a

UniProt

c.31A>T p.Thr11Ser missense variant moderate contig700 1945603

IGV: Start, Jump

T/A
NGS:
0.763
C90:
0.876
PKSG-2b

UniProt

c.1152T>A p.Asn384Lys missense variant moderate contig700 1950486

IGV: Start, Jump

A/T
NGS:
0.715
C90:
0.895
PKSG-2b

UniProt

c.1132C>G p.Leu378Val missense variant moderate contig700 1950506

IGV: Start, Jump

G/C
NGS:
0.717
C90:
0.000
PKSG-2b

UniProt

c.1117A>G p.Ile373Val missense variant moderate contig700 1950521

IGV: Start, Jump

T/C
NGS:
0.807
C90:
0.981
PKSG-2b

UniProt

c.948T>G p.Asp316Glu missense variant moderate contig700 1950690

IGV: Start, Jump

A/C
NGS:
0.456
C90:
0.000
PKSG-2b

UniProt

c.945T>G p.Ser315Arg missense variant moderate contig700 1950693

IGV: Start, Jump

A/C
NGS:
0.454
C90:
0.000
PKSG-2b

UniProt

c.774G>A p.Met258Ile missense variant moderate contig700 1950864

IGV: Start, Jump

C/T
NGS:
0.366
C90:
0.947
PKSG-2b

UniProt

c.31A>T p.Thr11Ser missense variant moderate contig700 1951851

IGV: Start, Jump

T/A
NGS:
0.844
C90:
0.880
DXR-2

UniProt

c.1319T>C p.Ile440Thr missense variant moderate contig380 285250

IGV: Start, Jump

A/G
NGS:
0.480
C90:
0.000
DXR-2

UniProt

c.431C>G p.Ala144Gly missense variant moderate contig380 287760

IGV: Start, Jump

G/C
NGS:
0.182
C90:
0.550
aPT4

UniProt

c.153A>C p.Lys51Asn missense variant moderate contig121 2828809

IGV: Start, Jump

A/C
NGS:
0.375
C90:
0.000
aPT4

UniProt

c.216A>T p.Lys72Asn missense variant moderate contig121 2828872

IGV: Start, Jump

A/T
NGS:
0.075
C90:
0.000
aPT4

UniProt

c.235_236delGT p.Val79fs frameshift variant high contig121 2829030

IGV: Start, Jump

ATG/A
NGS:
0.417
C90:
0.000
aPT4

UniProt

c.238delT p.Ser80fs frameshift variant high contig121 2829034

IGV: Start, Jump

AT/A
NGS:
0.421
C90:
0.000
aPT4

UniProt

c.302A>G p.Asn101Ser missense variant moderate contig121 2829099

IGV: Start, Jump

A/G
NGS:
0.349
C90:
0.000
aPT4

UniProt

c.1111C>A p.Leu371Ile missense variant moderate contig121 2833296

IGV: Start, Jump

C/A
NGS:
0.044
C90:
0.000
aPT4

UniProt

c.1168T>C p.Tyr390His missense variant moderate contig121 2833503

IGV: Start, Jump

T/C
NGS:
0.546
C90:
0.000
aPT1

UniProt

c.406A>G p.Ile136Val missense variant moderate contig121 2839605

IGV: Start, Jump

A/G
NGS:
0.579
C90:
0.761
aPT1

UniProt

c.629C>T p.Thr210Ile missense variant moderate contig121 2840237

IGV: Start, Jump

C/T
NGS:
0.561
C90:
0.598
HDS-2

UniProt

c.82_93delGTAACCGGAACT p.Val28_Thr31del conservative inframe deletion moderate contig95 1989748

IGV: Start, Jump

CGTAACCGGAACT/C
NGS:
0.805
C90:
0.000
HDS-2

UniProt

c.127T>G p.Ser43Ala missense variant moderate contig95 1989794

IGV: Start, Jump

T/G
NGS:
0.721
C90:
0.000
HDS-1

UniProt

c.-108+1_-108+2insG splice donor variant & intron variant high contig1891 889975

IGV: Start, Jump

A/AC
NGS:
0.217
C90:
0.000

Nearest genetic relatives (All Samples)

0 0.058 0.117 0.175 0.233
clone distance sibling distance more distant
  1. 0.172 RKM-2018-008 (RSP11099)
  2. 0.179 The Gift (RSP10988)
  3. 0.183 Tahoe OG (RSP11189)
  4. 0.196 Noetic OG (RSP11455)
  5. 0.198 NSPM1 (RSP11362)
  6. 0.202 Skywalker OG (RSP10837)
  7. 0.204 SFVxTK (RSP11072)
  8. 0.207 Casco Kush (RSP11167)
  9. 0.209 Pure Power Plant (RSP11265)
  10. 0.209 Rugburn OG (RSP11353)
  11. 0.209 SHERBERT (RSP11355)
  12. 0.213 501st OG (RSP11241)
  13. 0.218 Red Eye OG (RSP11190)
  14. 0.219 RKM-2018-013 (RSP11104)
  15. 0.220 Gorilla Cookies (RSP11231)
  16. 0.220 Serious Happiness (RSP10763)
  17. 0.227 RKM-2018-012 (RSP11103)
  18. 0.229 RKM-2018-032 (RSP11124)
  19. 0.231 RKM-2018-021 (RSP11113)
  20. 0.232 NSPM x NSPM (RSP11487)

Nearest genetic relatives (Base Tree)

0 0.083 0.167 0.250 0.333
clone distance sibling distance more distant
  1. 0.184 The Gift (RSP10988)
  2. 0.201 Skywalker OG (RSP10837)
  3. 0.234 RKM-2018-032 (RSP11124)
  4. 0.251 RKM-2018-034 (RSP11126)
  5. 0.256 Blueberry Cheesecake (RSP10684)
  6. 0.261 Pie Hoe (RSP11073)
  7. 0.277 RKM-2018-005 (RSP11096)
  8. 0.286 Liberty Haze (RSP11000)
  9. 0.288 RKM-2018-033 (RSP11125)
  10. 0.290 Kimbo Slice (RSP10997)
  11. 0.298 RKM-2018-002 (RSP11093)
  12. 0.299 RKM-2018-026 (RSP11118)
  13. 0.307 Hermaphrodite ResearchSample2 (RSP11050)
  14. 0.311 RKM-2018-020 (RSP11112)
  15. 0.321 QUEEN JESUS (RSP10105)
  16. 0.322 Recon (RSP10755)
  17. 0.325 RKM-2018-031 (RSP11123)
  18. 0.326 Durban Poison (RSP11014)
  19. 0.327 RKM-2018-019 (RSP11111)
  20. 0.327 RKM-2018-029 (RSP11121)

Most genetically distant strains (All Samples)

0 0.125 0.250 0.375 0.500
clone distance sibling distance more distant
  1. 0.471 Tanao Sri 46 (RSP11486)
  2. 0.466 JL yellow (RSP11075)
  3. 0.465 Tanao Sri-white 80 (RSP11621)
  4. 0.458 Cherry Blossom (RSP11306)
  5. 0.456 80E (RSP11213)
  6. 0.456 JL 3rd Gen Mother (RSP11214)
  7. 0.451 Unknown- Cherry Wine - 001 (RSP11268)
  8. 0.447 Squirrel Tail 81 (RSP11622)
  9. 0.443 Tiger Tail 30 (RSP11484)
  10. 0.441 Cherry Blossom (RSP11323)
  11. 0.440 AVIDEKEL 2 0 (RSP11174)
  12. 0.439 Cherry Blossom (RSP11325)
  13. 0.438 Danny Noonan (RSP11070)
  14. 0.435 Cbot-2019-004 (RSP11132)
  15. 0.433 80E (RSP11211)
  16. 0.428 Tanao Sri-white 79 (RSP11620)
  17. 0.427 Tiger Tail 78 (RSP11619)
  18. 0.427 JL 3rd Gen Mother (RSP11197)
  19. 0.427 80E (RSP11212)
  20. 0.426 JL 4th Gen 5 (RSP11199)

Most genetically distant strains (Base Tree)

0 0.117 0.233 0.350 0.467
clone distance sibling distance more distant
  1. 0.467 JL yellow (RSP11075)
  2. 0.441 Cbot-2019-004 (RSP11132)
  3. 0.423 Carmagnola (RSP11037)
  4. 0.423 Monoica (RSP10241)
  5. 0.417 Italian Kiss (RSP11034)
  6. 0.416 Feral (RSP10890)
  7. 0.414 Cbot-2019-005 (RSP11133)
  8. 0.408 Lovrin (RSP10658)
  9. 0.404 Cherry (RSP11143)
  10. 0.402 Fedora 17 (RSP10661)
  11. 0.399 Ivory (RSP10668)
  12. 0.398 RKM-2018-006 (RSP11097)
  13. 0.398 Kyrgyz Gold (RSP11054)
  14. 0.397 Futura 75 (RSP10664)
  15. 0.396 Santhica27 (RSP11047)
  16. 0.395 Carmagnola (RSP10979)
  17. 0.394 USO 31 (RSP10981)
  18. 0.393 Black Beauty (RSP11035)
  19. 0.388 Tisza (RSP11044)
  20. 0.388 RKM-2018-027 (RSP11119)

Nearest genetic relative in Phylos dataset

Phylos Strain SRR8346455
Overlapping SNPs:
85
Concordance:
62

Nearest genetic relative in Lynch dataset

Lynch Strain SRR3495242
Overlapping SNPs:
3
Concordance:
3

Blockchain Registration Information

Transaction ID
f6cbb15eee1273c6dcc4bc7353b220623941f0ff45b8f6a912c016b0068b1589
Stamping Certificate
Download PDF (39.5 KB)
SHASUM Hash
41677d3dc9f58a8c9db5e84bd0f304dfc5df3ef09097a6b0b4f14a8c3355d64c
QR code for RSP11638

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