1971/1974 Skunk

RSP 12498

Grower: Aficionado French Connection S.L

General Information

Accession Date
December 1, 2021
Reported Plant Sex
Female

The strain rarity visualization shows how distant the strain is from the other cultivars in the Kannapedia database. The y-axis represents genetic distance, getting farther as you go up. The width of the visualization at any position along the y-axis shows how many strains there are in the database at that genetic distance. So, a common strain will have a more bottom-heavy shape, while uncommon and rare cultivars will have a visualization that is generally shifted towards the top.

Rarity: Uncommon
Most Distant Most Similar

Chemical Information

Cannabinoid and terpenoid information provided by the grower.

Cannabinoids

No information provided.

Terpenoids

No information provided.

Genetic Information

Plant Type
Unknown

The bell curve in the heterozygosity visualization shows the distribution of heterozygosity levels for cannabis cultivars in the Kannapedia database. The green line shows where this particular strain fits within the distribution. Heterozygosity is associated with heterosis (aka hybrid vigor) but also leads to the production of more variable offspring. When plants have two genetically different parents, heterozygosity levels will be higher than if it has been inbred or backcrossed repeatedly.

Heterozygosity: 1.18%
Least Heterozygous Most Heterozygous

The ratio of reads mapped to Y-contigs to reads mapped to the whole Cannabis genome (Y-ratios) has been demonstrated to be strongly correlated with plant sex typing. This plot shows the distribution of Y-ratios for all samples in our database which were sequenced with the same method (panel or WGS) as this sample and where this sample falls in the distribution.

Y-Ratio Distribution: 0.0272
male female RSP12498

This chart represents the Illumina sequence coverage over the Bt/Bd allele. These are the three regions in the cannabis genome that impact THCA, CBDA, CBGA production. Coverage over the Active CBDAS gene is highly correlated with Type II and Type III plants as described by Etienne de Meijer. Coverage over the THCA gene is highly correlated with Type I and Type II plants but is anti-correlated with Type III plants. Type I plants require coverage over the inactive CBDA loci and no coverage over the Active CBDA gene. Lack of coverage over the Active CBDA and Active THCA allele are presumed to be Type IV plants (CBGA dominant). While deletions of entire THCAS and CBDAS genes are the most common Bt:Bd alleles observed, it is possible to have plants with these genes where functional expression of the enzyme is disrupted by deactivating point mutations (Kojoma et al. 2006).

Bt/Bd Allele Coverage

This chart represents the Illumina sequence coverage over the CBCA synthase gene.

CBCAS Coverage

Variants (THCAS, CBDAS, and CBCAS)

Gene HGVS.c HGVS.p Annotation Annotation Impact Contig Contig Pos Ref/Alt Var Freq
THCAS c.1625C>T p.Pro542Leu missense variant moderate contig741 4416203

IGV: Start, Jump

G/A
NGS:
0.018
C90:
0.000

Variants (Select Genes of Interest)

EMF1-2

UniProt

c.710A>C p.His237Pro missense variant moderate contig885 810

IGV: Start, Jump

A/C
NGS:
0.090
C90:
0.474
PHL-2 c.1096G>A p.Ala366Thr missense variant moderate contig2621 340374

IGV: Start, Jump

G/A
NGS:
0.090
C90:
0.531
PHL-2 c.2783G>A p.Ser928Asn missense variant moderate contig2621 342826

IGV: Start, Jump

G/A
NGS:
0.107
C90:
0.890
PHL-2 c.3467A>G p.Gln1156Arg missense variant moderate contig2621 343510

IGV: Start, Jump

A/G
NGS:
0.064
C90:
0.713
PKSG-2b

UniProt

c.31A>T p.Thr11Ser missense variant moderate contig700 1951851

IGV: Start, Jump

T/A
NGS:
0.844
C90:
0.880
PKSG-2b

UniProt

c.-2_1dupATA start lost & conservative inframe insertion high contig700 1951880

IGV: Start, Jump

A/ATAT
NGS:
0.410
C90:
0.000
PKSG-4b

UniProt

c.431T>G p.Val144Gly missense variant moderate contig700 2721242

IGV: Start, Jump

A/C
NGS:
0.575
C90:
0.679
PKSG-4b

UniProt

c.419A>G p.Asp140Gly missense variant moderate contig700 2721254

IGV: Start, Jump

T/C
NGS:
0.430
C90:
0.440
PKSG-4b

UniProt

c.316+2T>A splice donor variant & intron variant high contig700 2723818

IGV: Start, Jump

A/T
NGS:
0.070
C90:
0.689
DXR-2

UniProt

c.1319T>C p.Ile440Thr missense variant moderate contig380 285250

IGV: Start, Jump

A/G
NGS:
0.480
C90:
0.000
ELF3

UniProt

c.812G>C p.Gly271Ala missense variant moderate contig97 242518

IGV: Start, Jump

G/C
NGS:
0.114
C90:
0.938
ELF3

UniProt

c.1230-2_1230-1delAG splice acceptor variant & intron variant high contig97 243676

IGV: Start, Jump

TAG/T
NGS:
0.088
C90:
0.450
ELF3

UniProt

c.1466G>A p.Ser489Asn missense variant moderate contig97 244297

IGV: Start, Jump

G/A
NGS:
0.123
C90:
0.000
ELF3

UniProt

c.1966C>G p.Pro656Ala missense variant moderate contig97 244797

IGV: Start, Jump

C/G
NGS:
0.123
C90:
0.632
HDS-2

UniProt

c.82_93delGTAACCGGAACT p.Val28_Thr31del conservative inframe deletion moderate contig95 1989748

IGV: Start, Jump

CGTAACCGGAACT/C
NGS:
0.805
C90:
0.000
HDS-2

UniProt

c.127T>G p.Ser43Ala missense variant moderate contig95 1989794

IGV: Start, Jump

T/G
NGS:
0.721
C90:
0.000
AAE1-2

UniProt

c.884C>T p.Thr295Ile missense variant moderate contig81 209846

IGV: Start, Jump

C/T
NGS:
0.042
C90:
0.000
AAE1-2

UniProt

c.1006A>G p.Lys336Glu missense variant moderate contig81 209968

IGV: Start, Jump

A/G
NGS:
0.116
C90:
0.000
HDS-1

UniProt

c.1618A>G p.Ile540Val missense variant moderate contig1891 885936

IGV: Start, Jump

T/C
NGS:
0.099
C90:
0.861
HDS-1

UniProt

c.56C>G p.Ala19Gly missense variant moderate contig1891 889336

IGV: Start, Jump

G/C
NGS:
0.125
C90:
0.943
HDS-1

UniProt

c.35G>A p.Cys12Tyr missense variant moderate contig1891 889357

IGV: Start, Jump

C/T
NGS:
0.094
C90:
0.646
HDS-1

UniProt

c.-108+1_-108+2insG splice donor variant & intron variant high contig1891 889975

IGV: Start, Jump

A/AC
NGS:
0.217
C90:
0.000
EMF2

UniProt

c.1383-2delA splice acceptor variant & intron variant high contig954 3056567

IGV: Start, Jump

TA/T
NGS:
0.000
C90:
0.000
EMF2

UniProt

c.1772A>G p.Gln591Arg missense variant moderate contig954 3059929

IGV: Start, Jump

A/G
NGS:
0.092
C90:
0.876
FT

UniProt

c.334_339dupTGCTAT p.Cys112_Tyr113dup conservative inframe insertion moderate contig1561 3126370

IGV: Start, Jump

G/GTGCTAT
NGS:
0.009
C90:
0.153
AAE1-3

UniProt

c.722G>A p.Arg241Lys missense variant moderate contig976 1083132

IGV: Start, Jump

C/T
NGS:
0.070
C90:
0.000
AAE1-3

UniProt

c.634G>C p.Gly212Arg missense variant moderate contig976 1083220

IGV: Start, Jump

C/G
NGS:
0.116
C90:
0.000
AAE1-3

UniProt

c.501G>C p.Leu167Phe missense variant moderate contig976 1083524

IGV: Start, Jump

C/G
NGS:
0.004
C90:
0.000
AAE1-3

UniProt

c.199A>G p.Asn67Asp missense variant moderate contig976 1083876

IGV: Start, Jump

T/C
NGS:
0.079
C90:
0.000
AAE1-3

UniProt

c.188A>G p.Asn63Ser missense variant moderate contig976 1083887

IGV: Start, Jump

T/C
NGS:
0.077
C90:
0.000
GGR

UniProt

c.704A>T p.His235Leu missense variant moderate contig2282 549696

IGV: Start, Jump

A/T
NGS:
0.121
C90:
0.000

Nearest genetic relatives (All Samples)

0 0.050 0.100 0.150 0.200
clone distance sibling distance more distant
  1. 0.120 B52 (SRR14708255)
  2. 0.135 Mexican Sativa (SRR14708263)
  3. 0.151 Juicy Gummy x Royal Kush (RSP12484)
  4. 0.151 PCL1 (SRR14708246)
  5. 0.155 Cream Sugar (RSP12487)
  6. 0.156 Alpine Rocket (SRR14708266)
  7. 0.159 Kompolti (SRR14708277)
  8. 0.159 Urkle lime (RSP12485)
  9. 0.163 Durban Poison 1 (RSP11013)
  10. 0.164 Cherry Lime Runtz (RSP12486)
  11. 0.167 XUM1 (SRR14708205)
  12. 0.173 R2in135 (SRR14708236)
  13. 0.173 VIR 449 - Szegedi 9 (SRR14708213)
  14. 0.175 Fedora 17 (SRR14708222)
  15. 0.175 VIR 493 - Glukhovskaja 10 Zheltostebel'naja (SRR14708230)
  16. 0.176 Electra (RSP11366)
  17. 0.176 R1in136 (SRR14708225)
  18. 0.178 Big Skunk (SRR14708269)
  19. 0.178 Hindu Kush (SRR14708261)
  20. 0.179 VIR 483 (SRR14708240)

Nearest genetic relatives (Base Tree)

0 0.075 0.150 0.225 0.300
clone distance sibling distance more distant
  1. 0.214 UP Sunrise (RSP10989)
  2. 0.226 RKM-2018-023 (RSP11115)
  3. 0.229 Liberty Haze (RSP11000)
  4. 0.233 CST (RSP11002)
  5. 0.235 Durban Poison (RSP11014)
  6. 0.259 Cbot-2019-001 (RSP11129)
  7. 0.265 Blueberry Cheesecake (RSP10684)
  8. 0.267 Gold Cracker (RSP11048)
  9. 0.269 Golden Goat 2 (RSP10991)
  10. 0.271 RKM-2018-020 (RSP11112)
  11. 0.273 Cbot-2019-006 (RSP11134)
  12. 0.276 Blue Dream (RSP11033)
  13. 0.276 Pie Hoe (RSP11073)
  14. 0.280 Kimbo Slice (RSP10997)
  15. 0.283 Recon (RSP10755)
  16. 0.285 Skunk 18 (RSP11038)
  17. 0.289 The Gift (RSP10988)
  18. 0.290 RKM-2018-018 (RSP11110)
  19. 0.296 Tygra (RSP10667)
  20. 0.298 RKM-2018-027 (RSP11119)

Most genetically distant strains (All Samples)

0 0.117 0.233 0.350 0.467
clone distance sibling distance more distant
  1. 0.438 Feral (RSP11205)
  2. 0.423 80E (RSP11212)
  3. 0.419 80E (RSP11213)
  4. 0.415 Feral (RSP10890)
  5. 0.415 Carmagnola (RSP11202)
  6. 0.412 Feral (RSP10891)
  7. 0.407 Feral (RSP11206)
  8. 0.404 Feral (RSP10892)
  9. 0.397 80E (RSP11211)
  10. 0.393 Arcata Trainwreck (RSP11176)
  11. 0.389 Cherry Blossom (RSP11323)
  12. 0.388 Fatso (RSP11741)
  13. 0.385 Tiborszallasie (RSP11210)
  14. 0.382 Carmagnola (RSP11037)
  15. 0.381 Carmagnola USO 31 (RSP11204)
  16. 0.377 Carmagnola (RSP10655)
  17. 0.376 Fedora 17 (RSP11203)
  18. 0.375 CS (RSP11208)
  19. 0.373 Diana (RSP10235)
  20. 0.372 Cbot-2019-005 (RSP11133)

Most genetically distant strains (Base Tree)

0 0.108 0.217 0.325 0.433
clone distance sibling distance more distant
  1. 0.418 Feral (RSP10890)
  2. 0.397 Carmagnola (RSP11037)
  3. 0.388 RKM-2018-002 (RSP11093)
  4. 0.386 Cbot-2019-005 (RSP11133)
  5. 0.384 Monoica (RSP10241)
  6. 0.382 Carmagnola (RSP10979)
  7. 0.376 Tisza (RSP11044)
  8. 0.375 RKM-2018-009 (RSP11100)
  9. 0.374 Futura 75 (RSP10664)
  10. 0.372 KYRG-11 (RSP11051)
  11. 0.367 Fedora 17 (RSP10661)
  12. 0.366 Santhica27 (RSP11047)
  13. 0.364 Ivory (RSP10668)
  14. 0.363 RKM-2018-033 (RSP11125)
  15. 0.362 RKM-2018-034 (RSP11126)
  16. 0.358 Kush Hemp E1 (RSP11128)
  17. 0.355 Sour Raspberry (RSP10551)
  18. 0.350 Kyrgyz Gold (RSP11054)
  19. 0.349 USO 31 (RSP10981)
  20. 0.348 Jiangji (RSP10653)

Nearest genetic relative in Phylos dataset

Phylos Strain SRR8346889
Overlapping SNPs:
28
Concordance:
23

Nearest genetic relative in Lynch dataset

Lynch Strain SRR3495252
Overlapping SNPs:
6
Concordance:
6

Blockchain Registration Information

Transaction ID
7a5a2889f39db0e6623e8e90246c9d969c2eba40182edcc3de84988de327a041
Stamping Certificate
Download PDF (39.7 KB)
SHASUM Hash
17596c9c477a565c251f60a92641d34c10fcf8cf4e0b0b6fa3ae8d2779e6972f
QR code for RSP12498

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