S21-002-01

RSP 12656

Grower: Humboldt DNA

General Information

Accession Date
July 20, 2022
Reported Plant Sex
not reported

The strain rarity visualization shows how distant the strain is from the other cultivars in the Kannapedia database. The y-axis represents genetic distance, getting farther as you go up. The width of the visualization at any position along the y-axis shows how many strains there are in the database at that genetic distance. So, a common strain will have a more bottom-heavy shape, while uncommon and rare cultivars will have a visualization that is generally shifted towards the top.

Rarity: Rare
Most Distant Most Similar

Chemical Information

Cannabinoid and terpenoid information provided by the grower.

Cannabinoids

No information provided.

Terpenoids

No information provided.

Genetic Information

Plant Type
Type I

The bell curve in the heterozygosity visualization shows the distribution of heterozygosity levels for cannabis cultivars in the Kannapedia database. The green line shows where this particular strain fits within the distribution. Heterozygosity is associated with heterosis (aka hybrid vigor) but also leads to the production of more variable offspring. When plants have two genetically different parents, heterozygosity levels will be higher than if it has been inbred or backcrossed repeatedly.

Heterozygosity: 1.14%
Least Heterozygous Most Heterozygous

The ratio of reads mapped to Y-contigs to reads mapped to the whole Cannabis genome (Y-ratios) has been demonstrated to be strongly correlated with plant sex typing. This plot shows the distribution of Y-ratios for all samples in our database which were sequenced with the same method (panel or WGS) as this sample and where this sample falls in the distribution.

Y-Ratio Distribution: 0.1050
male female RSP12656

This chart represents the Illumina sequence coverage over the Bt/Bd allele. These are the three regions in the cannabis genome that impact THCA, CBDA, CBGA production. Coverage over the Active CBDAS gene is highly correlated with Type II and Type III plants as described by Etienne de Meijer. Coverage over the THCA gene is highly correlated with Type I and Type II plants but is anti-correlated with Type III plants. Type I plants require coverage over the inactive CBDA loci and no coverage over the Active CBDA gene. Lack of coverage over the Active CBDA and Active THCA allele are presumed to be Type IV plants (CBGA dominant). While deletions of entire THCAS and CBDAS genes are the most common Bt:Bd alleles observed, it is possible to have plants with these genes where functional expression of the enzyme is disrupted by deactivating point mutations (Kojoma et al. 2006).

Bt/Bd Allele Coverage

This chart represents the Illumina sequence coverage over the CBCA synthase gene.

CBCAS Coverage

Variants (THCAS, CBDAS, and CBCAS)

Gene HGVS.c HGVS.p Annotation Annotation Impact Contig Contig Pos Ref/Alt Var Freq
THCAS c.749C>A p.Ala250Asp missense variant moderate contig741 4417079

IGV: Start, Jump

G/T
NGS:
0.127
C90:
0.632

Variants (Select Genes of Interest)

PHL-2 c.2933G>T p.Arg978Leu missense variant moderate contig2621 342976

IGV: Start, Jump

G/T
NGS:
0.048
C90:
0.297
PHL-2 c.2936T>G p.Val979Gly missense variant moderate contig2621 342979

IGV: Start, Jump

T/G
NGS:
0.048
C90:
0.000
PHL-2 c.3209A>G p.Gln1070Arg missense variant moderate contig2621 343252

IGV: Start, Jump

A/G
NGS:
0.103
C90:
0.885
PHL-2 c.3467A>G p.Gln1156Arg missense variant moderate contig2621 343510

IGV: Start, Jump

A/G
NGS:
0.064
C90:
0.713
PKSG-4a

UniProt

c.617A>G p.Tyr206Cys missense variant moderate contig700 1938028

IGV: Start, Jump

A/G
NGS:
0.075
C90:
0.000
PKSG-4a

UniProt

c.626_628delATA p.Asn209del disruptive inframe deletion moderate contig700 1938032

IGV: Start, Jump

CAAT/C
NGS:
0.075
C90:
0.000
PKSG-4b

UniProt

c.489delT p.Phe163fs frameshift variant high contig700 2721183

IGV: Start, Jump

CA/C
NGS:
0.792
C90:
0.761
FAD2-2

UniProt

c.161T>A p.Leu54His missense variant moderate contig83 1803208

IGV: Start, Jump

A/T
NGS:
0.156
C90:
0.000
FAD2-2

UniProt

c.64G>T p.Ala22Ser missense variant moderate contig83 1803305

IGV: Start, Jump

C/A
NGS:
0.048
C90:
0.000
ELF3

UniProt

c.358G>A p.Gly120Arg missense variant moderate contig97 242064

IGV: Start, Jump

G/A
NGS:
0.099
C90:
0.493
ELF3

UniProt

c.752G>T p.Gly251Val missense variant moderate contig97 242458

IGV: Start, Jump

G/T
NGS:
0.022
C90:
0.139
ELF3

UniProt

c.772A>G p.Ser258Gly missense variant moderate contig97 242478

IGV: Start, Jump

A/G
NGS:
0.112
C90:
0.000
ELF3

UniProt

c.812G>C p.Gly271Ala missense variant moderate contig97 242518

IGV: Start, Jump

G/C
NGS:
0.114
C90:
0.938
ELF3

UniProt

c.1630A>G p.Thr544Ala missense variant moderate contig97 244461

IGV: Start, Jump

A/G
NGS:
0.103
C90:
0.545
ELF3

UniProt

c.1803_1805delTCA p.His601del disruptive inframe deletion moderate contig97 244625

IGV: Start, Jump

ACAT/A
NGS:
0.092
C90:
0.000
ELF3

UniProt

c.1966C>G p.Pro656Ala missense variant moderate contig97 244797

IGV: Start, Jump

C/G
NGS:
0.123
C90:
0.632
ELF3

UniProt

c.2141C>G p.Pro714Arg missense variant moderate contig97 244972

IGV: Start, Jump

C/G
NGS:
0.088
C90:
0.335
HDS-2

UniProt

c.82_93delGTAACCGGAACT p.Val28_Thr31del conservative inframe deletion moderate contig95 1989748

IGV: Start, Jump

CGTAACCGGAACT/C
NGS:
0.805
C90:
0.000
HDS-2

UniProt

c.127T>G p.Ser43Ala missense variant moderate contig95 1989794

IGV: Start, Jump

T/G
NGS:
0.721
C90:
0.000
PHL-1

UniProt

c.2623A>G p.Thr875Ala missense variant moderate contig1439 1487174

IGV: Start, Jump

T/C
NGS:
0.123
C90:
0.000
PHL-1

UniProt

c.1387A>G p.Thr463Ala missense variant moderate contig1439 1489811

IGV: Start, Jump

T/C
NGS:
0.107
C90:
0.904
PIE1-2

UniProt

c.1289A>G p.Asp430Gly missense variant moderate contig1460 1192109

IGV: Start, Jump

T/C
NGS:
0.079
C90:
0.000
FLD

UniProt

c.2981T>C p.Met994Thr missense variant moderate contig1450 2044012

IGV: Start, Jump

A/G
NGS:
0.064
C90:
0.478
FLD

UniProt

c.2964C>A p.Asp988Glu missense variant moderate contig1450 2044029

IGV: Start, Jump

G/T
NGS:
0.057
C90:
0.464
FLD

UniProt

c.125G>A p.Ser42Asn missense variant moderate contig1450 2047909

IGV: Start, Jump

C/T
NGS:
0.037
C90:
0.388
PIE1-1

UniProt

c.1222C>G p.Gln408Glu missense variant moderate contig1225 2281482

IGV: Start, Jump

C/G
NGS:
0.096
C90:
0.852
PIE1-1

UniProt

c.6725C>T p.Ala2242Val missense variant moderate contig1225 2289290

IGV: Start, Jump

C/T
NGS:
0.046
C90:
0.737
GGR

UniProt

c.456T>A p.His152Gln missense variant moderate contig2282 549448

IGV: Start, Jump

T/A
NGS:
0.068
C90:
0.000
GGR

UniProt

c.460G>A p.Asp154Asn missense variant moderate contig2282 549452

IGV: Start, Jump

G/A
NGS:
0.070
C90:
0.000

Nearest genetic relatives (All Samples)

0 0.067 0.133 0.200 0.267
clone distance sibling distance more distant
  1. 0.195 A15 (RSP12655)
  2. 0.198 A13 (RSP12654)
  3. 0.213 SHERBERT (RSP11355)
  4. 0.220 Lemon OG Haze (RSP11977)
  5. 0.229 RKM-2018-021 (RSP11113)
  6. 0.229 RKM-2018-032 (RSP11124)
  7. 0.231 RKM-2018-017 (RSP11109)
  8. 0.236 Skywalker OG (RSP10837)
  9. 0.239 Thank You Jerry (RSP11459)
  10. 0.243 Tahoe OG (RSP11189)
  11. 0.243 SFVxTK (RSP11072)
  12. 0.243 GG4 (RSP12096)
  13. 0.245 C Temple (RSP11643)
  14. 0.249 GG 4 (RSP11461)
  15. 0.252 501st OG (RSP11241)
  16. 0.252 RKM-2018-026 (RSP11118)
  17. 0.253 NSPM1 (RSP11362)
  18. 0.254 GG4 (RSP11978)
  19. 0.254 Super Sour Diesel (RSP11191)
  20. 0.255 Old Family Purple (RSP12098)

Nearest genetic relatives (Base Tree)

0 0.083 0.167 0.250 0.333
clone distance sibling distance more distant
  1. 0.236 RKM-2018-032 (RSP11124)
  2. 0.241 Skywalker OG (RSP10837)
  3. 0.264 RKM-2018-026 (RSP11118)
  4. 0.267 The Gift (RSP10988)
  5. 0.271 RKM-2018-033 (RSP11125)
  6. 0.274 RKM-2018-034 (RSP11126)
  7. 0.277 Liberty Haze (RSP11000)
  8. 0.283 RKM-2018-002 (RSP11093)
  9. 0.284 Blueberry Cheesecake (RSP10684)
  10. 0.289 Pie Hoe (RSP11073)
  11. 0.293 Kush Hemp E1 (RSP11128)
  12. 0.297 Gold Cracker (RSP11048)
  13. 0.300 RKM-2018-009 (RSP11100)
  14. 0.302 Blueberry Cheesecake (RSP10680)
  15. 0.303 Hermaphrodite Research Sample1 (RSP11049)
  16. 0.306 Kimbo Slice (RSP10997)
  17. 0.306 Blue Dream (RSP11033)
  18. 0.308 Golden Goat 2 (RSP10991)
  19. 0.310 RKM-2018-020 (RSP11112)
  20. 0.314 Tisza (RSP10659)

Most genetically distant strains (All Samples)

0 0.125 0.250 0.375 0.500
clone distance sibling distance more distant
  1. 0.500 Cherry Blossom (RSP11323)
  2. 0.488 Unknown- Cherry Wine - 003 (RSP11270)
  3. 0.488 Cherry Blossom (RSP11328)
  4. 0.485 Cherry Blossom (RSP11298)
  5. 0.482 Unknown- Cherry Wine - 001 (RSP11268)
  6. 0.475 Cherry Blossom (RSP11311)
  7. 0.467 Cherry Blossom (RSP11334)
  8. 0.462 Unknown- Cherry Wine - 002 (RSP11269)
  9. 0.456 Cherry Blossom (RSP11309)
  10. 0.453 Cherry Blossom (RSP11312)
  11. 0.452 Cherry Blossom (RSP11318)
  12. 0.451 Northern Skunk (RSP11456)
  13. 0.447 Cherry Blossom (RSP11317)
  14. 0.445 Cherry Fog XL (RSP11458)
  15. 0.442 Tanao Sri 46 (RSP11486)
  16. 0.439 Unknown- Cherry Wine - 004 (RSP11271)
  17. 0.437 JL 3rd Gen Father (RSP11196)
  18. 0.437 Avidekel (RSP10938)
  19. 0.433 IUP3 (SRR14708256)
  20. 0.431 Northern Lights (RSP11501)

Most genetically distant strains (Base Tree)

0 0.108 0.217 0.325 0.433
clone distance sibling distance more distant
  1. 0.420 Cbot-2019-005 (RSP11133)
  2. 0.402 JL yellow (RSP11075)
  3. 0.392 Feral (RSP10890)
  4. 0.392 Monoica (RSP10241)
  5. 0.390 Cherry (RSP11143)
  6. 0.377 Carmagnola (RSP11037)
  7. 0.375 RKM-2018-018 (RSP11110)
  8. 0.374 Carmagnola (RSP10979)
  9. 0.373 Cherry (RSP11142)
  10. 0.372 Italian Kiss (RSP11034)
  11. 0.372 Santhica27 (RSP11047)
  12. 0.371 RKM-2018-003 (RSP11094)
  13. 0.369 USO 31 (RSP10981)
  14. 0.369 RKM-2018-022 (RSP11114)
  15. 0.368 KYRG-11 (RSP11051)
  16. 0.366 Cbot-2019-004 (RSP11132)
  17. 0.366 RKM-2018-023 (RSP11115)
  18. 0.366 Jiangji (RSP10653)
  19. 0.364 Tygra (RSP10667)
  20. 0.363 Skunk 18 (RSP11038)

Nearest genetic relative in Phylos dataset

Phylos Strain SRR4451110
Overlapping SNPs:
39
Concordance:
29

Nearest genetic relative in Lynch dataset

Lynch Strain SRR3495198
Overlapping SNPs:
6
Concordance:
6

Blockchain Registration Information

Transaction ID
369801626749c64e453e9aad61e13ada8fd6e158dd09c611af376248938842c0
Stamping Certificate
Download PDF (39.5 KB)
SHASUM Hash
30dae169019171c3df88ecf37d4b2589535d21c4143886b67397e2daa142fa9a
QR code for RSP12656

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