Blueberry Rainbow Belts #1

RSP 12798

Grower: Devi

General Information

Sample Name
Blueberry Rainbow Belts #1-20221019
Accession Date
October 19, 2022
Reported Plant Sex
Female
DNA Extracted From
Unknown

The strain rarity visualization shows how distant the strain is from the other cultivars in the Kannapedia database. The y-axis represents genetic distance, getting farther as you go up. The width of the visualization at any position along the y-axis shows how many strains there are in the database at that genetic distance. So, a common strain will have a more bottom-heavy shape, while uncommon and rare cultivars will have a visualization that is generally shifted towards the top.

Rarity: Rare
Most Distant Most Similar

Chemical Information

Cannabinoid and terpenoid information provided by the grower.

Cannabinoids

No information provided.

Terpenoids

No information provided.

Genetic Information

Plant Type
Type I

The bell curve in the heterozygosity visualization shows the distribution of heterozygosity levels for cannabis cultivars in the Kannapedia database. The green line shows where this particular strain fits within the distribution. Heterozygosity is associated with heterosis (aka hybrid vigor) but also leads to the production of more variable offspring. When plants have two genetically different parents, heterozygosity levels will be higher than if it has been inbred or backcrossed repeatedly.

Heterozygosity: 0.95%
Least Heterozygous Most Heterozygous

The ratio of reads mapped to Y-contigs to reads mapped to the whole Cannabis genome (Y-ratios) has been demonstrated to be strongly correlated with plant sex typing. This plot shows the distribution of Y-ratios for all samples in our database which were sequenced with the same method (panel or WGS) as this sample and where this sample falls in the distribution.

Y-Ratio Distribution: 0.0630
male female RSP12798

This chart represents the Illumina sequence coverage over the Bt/Bd allele. These are the three regions in the cannabis genome that impact THCA, CBDA, CBGA production. Coverage over the Active CBDAS gene is highly correlated with Type II and Type III plants as described by Etienne de Meijer. Coverage over the THCA gene is highly correlated with Type I and Type II plants but is anti-correlated with Type III plants. Type I plants require coverage over the inactive CBDA loci and no coverage over the Active CBDA gene. Lack of coverage over the Active CBDA and Active THCA allele are presumed to be Type IV plants (CBGA dominant). While deletions of entire THCAS and CBDAS genes are the most common Bt:Bd alleles observed, it is possible to have plants with these genes where functional expression of the enzyme is disrupted by deactivating point mutations (Kojoma et al. 2006).

Bt/Bd Allele Coverage

This chart represents the Illumina sequence coverage over the CBCA synthase gene.

CBCAS Coverage

Variants (THCAS, CBDAS, and CBCAS)

Gene HGVS.c HGVS.p Annotation Annotation Impact Contig Contig Pos Ref/Alt Var Freq
THCAS c.998C>G p.Pro333Arg missense variant moderate contig741 4416830

IGV: Start, Jump

G/C
NGS:
0.182
C90:
0.000

Variants (Select Genes of Interest)

PKSG-4b

UniProt

c.535_545delATTGGAGTGGG p.Ile179fs frameshift variant high contig700 2721127

IGV: Start, Jump

CCCCACTCCAAT/C
NGS:
0.105
C90:
0.000
PKSG-4b

UniProt

c.523C>T p.His175Tyr missense variant moderate contig700 2721150

IGV: Start, Jump

G/A
NGS:
0.112
C90:
0.325
PKSG-4b

UniProt

c.489delT p.Phe163fs frameshift variant high contig700 2721183

IGV: Start, Jump

CA/C
NGS:
0.792
C90:
0.761
PKSG-4b

UniProt

c.353_354insCC p.Gly119fs frameshift variant high contig700 2721319

IGV: Start, Jump

T/TGG
NGS:
0.175
C90:
0.000
FAD2-2

UniProt

c.172G>T p.Asp58Tyr missense variant moderate contig83 1803197

IGV: Start, Jump

C/A
NGS:
0.070
C90:
0.000
FAD2-2

UniProt

c.161T>A p.Leu54His missense variant moderate contig83 1803208

IGV: Start, Jump

A/T
NGS:
0.156
C90:
0.000
ELF3

UniProt

c.358G>A p.Gly120Arg missense variant moderate contig97 242064

IGV: Start, Jump

G/A
NGS:
0.099
C90:
0.493
ELF3

UniProt

c.772A>G p.Ser258Gly missense variant moderate contig97 242478

IGV: Start, Jump

A/G
NGS:
0.112
C90:
0.000
ELF3

UniProt

c.1803_1805delTCA p.His601del disruptive inframe deletion moderate contig97 244625

IGV: Start, Jump

ACAT/A
NGS:
0.092
C90:
0.000
ELF3

UniProt

c.2198G>T p.Arg733Leu missense variant moderate contig97 245029

IGV: Start, Jump

G/T
NGS:
0.125
C90:
0.531
aPT1

UniProt

c.406A>G p.Ile136Val missense variant moderate contig121 2839605

IGV: Start, Jump

A/G
NGS:
0.579
C90:
0.761
HDS-2

UniProt

c.82_93delGTAACCGGAACT p.Val28_Thr31del conservative inframe deletion moderate contig95 1989748

IGV: Start, Jump

CGTAACCGGAACT/C
NGS:
0.805
C90:
0.000
PHL-1

UniProt

c.1387A>G p.Thr463Ala missense variant moderate contig1439 1489811

IGV: Start, Jump

T/C
NGS:
0.107
C90:
0.904
HDS-1

UniProt

c.2141G>C p.Gly714Ala missense variant moderate contig1891 884225

IGV: Start, Jump

C/G
NGS:
0.018
C90:
0.000
HDS-1

UniProt

c.56C>G p.Ala19Gly missense variant moderate contig1891 889336

IGV: Start, Jump

G/C
NGS:
0.125
C90:
0.943
HDS-1

UniProt

c.35G>A p.Cys12Tyr missense variant moderate contig1891 889357

IGV: Start, Jump

C/T
NGS:
0.094
C90:
0.646
EMF2

UniProt

c.1772A>G p.Gln591Arg missense variant moderate contig954 3059929

IGV: Start, Jump

A/G
NGS:
0.092
C90:
0.876
PIE1-1

UniProt

c.1222C>G p.Gln408Glu missense variant moderate contig1225 2281482

IGV: Start, Jump

C/G
NGS:
0.096
C90:
0.852
PIE1-1

UniProt

c.6725C>T p.Ala2242Val missense variant moderate contig1225 2289290

IGV: Start, Jump

C/T
NGS:
0.046
C90:
0.737
GGR

UniProt

c.456T>A p.His152Gln missense variant moderate contig2282 549448

IGV: Start, Jump

T/A
NGS:
0.068
C90:
0.000
GGR

UniProt

c.460G>A p.Asp154Asn missense variant moderate contig2282 549452

IGV: Start, Jump

G/A
NGS:
0.070
C90:
0.000

Nearest genetic relatives (All Samples)

0 0.075 0.150 0.225 0.300
closely related moderately related distantly related
  1. 0.216 The Gift (RSP10988)
  2. 0.220 Rainbow Belts 3 0 11 (RSP12801)
  3. 0.221 Animal Tree (RSP12791)
  4. 0.222 Animal Tree x Skittles 4 (RSP12792)
  5. 0.225 Purple Pakistani (RSP12800)
  6. 0.233 RKM-2018-008 (RSP11099)
  7. 0.235 Rainbow Belts 2 0 (RSP12802)
  8. 0.235 Animal Tree x Skittles 2 (RSP12793)
  9. 0.254 Eucalyptus (RSP12803)
  10. 0.256 SFVxTK (RSP11072)
  11. 0.256 El Presidente (RSP12807)
  12. 0.260 RKM-2018-017 (RSP11109)
  13. 0.261 Goin Bananas (RSP12509)
  14. 0.262 RKM-2018-032 (RSP11124)
  15. 0.262 RKM-2018-021 (RSP11113)
  16. 0.263 S21-002-01 (RSP12656)
  17. 0.264 Zombie Presidente (RSP12808)
  18. 0.265 Kush Hemp E1 (RSP11128)
  19. 0.267 Wedding Cake x MAC (RSP11464)
  20. 0.268 GMO x Poison Momosa (RSP12500)

Most genetically distant strains (All Samples)

0 0.125 0.250 0.375 0.500
closely related moderately related distantly related
  1. 0.478 Cherry Blossom (RSP11323)
  2. 0.456 Cherry Blossom (RSP11298)
  3. 0.450 Cherry Blossom (RSP11312)
  4. 0.447 80E (RSP11213)
  5. 0.446 Cherry Blossom (RSP11318)
  6. 0.445 Cherry Blossom (RSP11328)
  7. 0.441 Unknown- Cherry Wine - 003 (RSP11270)
  8. 0.440 Cherry Blossom (RSP11309)
  9. 0.440 Cherry Blossom (RSP11306)
  10. 0.438 Chematonic Cannatonic x Chemdawg (RSP11394)
  11. 0.437 Unknown- Cherry Wine - 001 (RSP11268)
  12. 0.433 Tanao Sri 46 (RSP11486)
  13. 0.428 Cherry Blossom (RSP11327)
  14. 0.428 Cherry Blossom (RSP11322)
  15. 0.427 Unknown- Cherry Wine - 002 (RSP11269)
  16. 0.425 Cherry Blossom (RSP11274)
  17. 0.425 BagSeed (RSP12627)
  18. 0.421 Cherry Blossom (RSP11330)
  19. 0.421 Avidekel (RSP10938)
  20. 0.421 Cherry Blossom (RSP11311)

Nearest genetic relative in Phylos dataset

Phylos Strain SRR8347159
Overlapping SNPs:
82
Concordance:
49

Nearest genetic relative in Lynch dataset

Lynch Strain SRR3495315
Overlapping SNPs:
11
Concordance:
11

Blockchain Registration Information

Transaction ID
515e4efa850776ec7314a31558fadd7e20b0fa96d3a928c6d9941d72d1ecff60
Stamping Certificate
Download PDF (39.7 KB)
SHASUM Hash
05c33eaa3aaca488dd92f17ac75a642a664be88a7163424c903d2cb04157f57b
QR code for RSP12798

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