DB

RSP 12936

Grower: Zamir Punja

General Information

Sample Name
DB - roots from flowering plant
Accession Date
September 27, 2023
Reported Plant Sex
Female
Microbiome
Krona Plot

The strain rarity visualization shows how distant the strain is from the other cultivars in the Kannapedia database. The y-axis represents genetic distance, getting farther as you go up. The width of the visualization at any position along the y-axis shows how many strains there are in the database at that genetic distance. So, a common strain will have a more bottom-heavy shape, while uncommon and rare cultivars will have a visualization that is generally shifted towards the top.

Rarity: Uncommon
Most Distant Most Similar

Chemical Information

Cannabinoid and terpenoid information provided by the grower.

Cannabinoids

No information provided.

Terpenoids

No information provided.

Genetic Information

Plant Type
Type I

The bell curve in the heterozygosity visualization shows the distribution of heterozygosity levels for cannabis cultivars in the Kannapedia database. The green line shows where this particular strain fits within the distribution. Heterozygosity is associated with heterosis (aka hybrid vigor) but also leads to the production of more variable offspring. When plants have two genetically different parents, heterozygosity levels will be higher than if it has been inbred or backcrossed repeatedly.

Heterozygosity: 1.28%
Least Heterozygous Most Heterozygous

The ratio of reads mapped to Y-contigs to reads mapped to the whole Cannabis genome (Y-ratios) has been demonstrated to be strongly correlated with plant sex typing. This plot shows the distribution of Y-ratios for all samples in our database which were sequenced with the same method (panel or WGS) as this sample and where this sample falls in the distribution.

Y-Ratio Distribution: 0.0275
male female RSP12936

This chart represents the Illumina sequence coverage over the Bt/Bd allele. These are the three regions in the cannabis genome that impact THCA, CBDA, CBGA production. Coverage over the Active CBDAS gene is highly correlated with Type II and Type III plants as described by Etienne de Meijer. Coverage over the THCA gene is highly correlated with Type I and Type II plants but is anti-correlated with Type III plants. Type I plants require coverage over the inactive CBDA loci and no coverage over the Active CBDA gene. Lack of coverage over the Active CBDA and Active THCA allele are presumed to be Type IV plants (CBGA dominant). While deletions of entire THCAS and CBDAS genes are the most common Bt:Bd alleles observed, it is possible to have plants with these genes where functional expression of the enzyme is disrupted by deactivating point mutations (Kojoma et al. 2006).

Bt/Bd Allele Coverage

This chart represents the Illumina sequence coverage over the CBCA synthase gene.

CBCAS Coverage

Variants (THCAS, CBDAS, and CBCAS)

No variants to report

Variants (Select Genes of Interest)

PHL-2 c.2783G>A p.Ser928Asn missense variant moderate contig2621 342826

IGV: Start, Jump

G/A
NGS:
0.107
C90:
0.890
PHL-2 c.3467A>G p.Gln1156Arg missense variant moderate contig2621 343510

IGV: Start, Jump

A/G
NGS:
0.064
C90:
0.713
DXR-2

UniProt

c.1319T>C p.Ile440Thr missense variant moderate contig380 285250

IGV: Start, Jump

A/G
NGS:
0.480
C90:
0.000
FAD2-2

UniProt

c.110C>T p.Ala37Val missense variant moderate contig83 1803259

IGV: Start, Jump

G/A
NGS:
0.013
C90:
0.000
FAD2-2

UniProt

c.62C>T p.Pro21Leu missense variant moderate contig83 1803307

IGV: Start, Jump

G/A
NGS:
0.042
C90:
0.000
ELF3

UniProt

c.772A>G p.Ser258Gly missense variant moderate contig97 242478

IGV: Start, Jump

A/G
NGS:
0.112
C90:
0.000
ELF3

UniProt

c.812G>C p.Gly271Ala missense variant moderate contig97 242518

IGV: Start, Jump

G/C
NGS:
0.114
C90:
0.938
ELF3

UniProt

c.1966C>G p.Pro656Ala missense variant moderate contig97 244797

IGV: Start, Jump

C/G
NGS:
0.123
C90:
0.632
ELF3

UniProt

c.2141C>G p.Pro714Arg missense variant moderate contig97 244972

IGV: Start, Jump

C/G
NGS:
0.088
C90:
0.335
HDS-2

UniProt

c.82_93delGTAACCGGAACT p.Val28_Thr31del conservative inframe deletion moderate contig95 1989748

IGV: Start, Jump

CGTAACCGGAACT/C
NGS:
0.805
C90:
0.000
HDS-2

UniProt

c.127T>G p.Ser43Ala missense variant moderate contig95 1989794

IGV: Start, Jump

T/G
NGS:
0.721
C90:
0.000
FAD4

UniProt

c.220C>G p.Arg74Gly missense variant moderate contig784 1690972

IGV: Start, Jump

C/G
NGS:
0.026
C90:
0.000
PIE1-2

UniProt

c.1289A>G p.Asp430Gly missense variant moderate contig1460 1192109

IGV: Start, Jump

T/C
NGS:
0.079
C90:
0.000
FLD

UniProt

c.125G>A p.Ser42Asn missense variant moderate contig1450 2047909

IGV: Start, Jump

C/T
NGS:
0.037
C90:
0.388
AAE1-3

UniProt

c.722G>A p.Arg241Lys missense variant moderate contig976 1083132

IGV: Start, Jump

C/T
NGS:
0.070
C90:
0.000
AAE1-3

UniProt

c.659G>A p.Arg220Gln missense variant moderate contig976 1083195

IGV: Start, Jump

C/T
NGS:
0.077
C90:
0.000
AAE1-3

UniProt

c.634G>C p.Gly212Arg missense variant moderate contig976 1083220

IGV: Start, Jump

C/G
NGS:
0.116
C90:
0.000
PIE1-1

UniProt

c.1222C>G p.Gln408Glu missense variant moderate contig1225 2281482

IGV: Start, Jump

C/G
NGS:
0.096
C90:
0.852

Nearest genetic relatives (All Samples)

0 0.058 0.117 0.175 0.233
closely related moderately related distantly related
  1. 0.092 DB (RSP12930)
  2. 0.186 Black Triangle (RSP11638)
  3. 0.193 The Gift (RSP10988)
  4. 0.201 501st OG (RSP11241)
  5. 0.201 PK (RSP12926)
  6. 0.210 Skywalker OG (RSP10837)
  7. 0.215 RKM-2018-021 (RSP11113)
  8. 0.215 RKM-2018-032 (RSP11124)
  9. 0.216 RKM-2018-008 (RSP11099)
  10. 0.217 GG 4 (RSP11461)
  11. 0.217 RKM-2018-017 (RSP11109)
  12. 0.217 RKM-2018-012 (RSP11103)
  13. 0.217 Animal Tree x [REDACTED] 4 (RSP12792)
  14. 0.219 PK (RSP12932)
  15. 0.224 SHERBERT (RSP11355)
  16. 0.224 Rugburn OG (RSP11353)
  17. 0.224 GG4 (RSP12096)
  18. 0.225 SFVxTK (RSP11072)
  19. 0.226 GG4 (RSP11978)
  20. 0.228 Tahoe OG (RSP11189)

Most genetically distant strains (All Samples)

0 0.125 0.250 0.375 0.500
closely related moderately related distantly related
  1. 0.468 Jamaican Lion (RSP12916)
  2. 0.467 Unknown- Cherry Wine - 003 (RSP11270)
  3. 0.461 JL yellow (RSP11075)
  4. 0.461 JL 3rd Gen Mother (RSP11214)
  5. 0.460 Cherry Blossom (RSP11317)
  6. 0.460 Unknown- Cherry Wine - 001 (RSP11268)
  7. 0.458 Jamaican Lion (RSP12915)
  8. 0.458 Jamaican Lion (RSP12917)
  9. 0.458 Tiger Tail 30 (RSP11484)
  10. 0.456 Unknown- Cherry Wine - 004 (RSP11271)
  11. 0.454 Tanao Sri 46 (RSP11486)
  12. 0.453 Cherry Blossom (RSP11311)
  13. 0.452 Unknown- Cherry Wine - 002 (RSP11269)
  14. 0.448 Jamaican Lion (RSP12913)
  15. 0.446 Cherry Blossom (RSP11298)
  16. 0.446 Cherry Fog XL (RSP11458)
  17. 0.445 Squirrel Tail 31 (RSP11485)
  18. 0.444 Cherry Blossom (RSP11308)
  19. 0.443 Northern Skunk (RSP11456)
  20. 0.443 Cherry Blossom (RSP11335)

Nearest genetic relative in Phylos dataset

Phylos Strain SRR8349242
Overlapping SNPs:
61
Concordance:
40

Nearest genetic relative in Lynch dataset

Lynch Strain SRR3495250
Overlapping SNPs:
2
Concordance:
2

Blockchain Registration Information

Transaction ID
2dc469e58d7825264844dc24324cf41af0d07075e8562e60af1f7ca99dd1efa8
Stamping Certificate
Download PDF (39.5 KB)
SHASUM Hash
79c7b2cb90779596a66a1a969384360a517890e8ef174af86288ff4b3de2eec1
QR code for RSP12936

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