Cbot-2019-005

RSP 11133

Grower: Research Development Cannabinoid

General Information

Accession Date
February 5, 2019
Reported Plant Sex
not reported

The strain rarity visualization shows how distant the strain is from the other cultivars in the Kannapedia database. The y-axis represents genetic distance, getting farther as you go up. The width of the visualization at any position along the y-axis shows how many strains there are in the database at that genetic distance. So, a common strain will have a more bottom-heavy shape, while uncommon and rare cultivars will have a visualization that is generally shifted towards the top.

Rarity: Rare
Most Distant Most Similar

Chemical Information

Cannabinoid and terpenoid information provided by the grower.

Cannabinoids

No information provided.

Terpenoids

No information provided.

Genetic Information

Plant Type
Type III

The bell curve in the heterozygosity visualization shows the distribution of heterozygosity levels for cannabis cultivars in the Kannapedia database. The green line shows where this particular strain fits within the distribution. Heterozygosity is associated with heterosis (aka hybrid vigor) but also leads to the production of more variable offspring. When plants have two genetically different parents, heterozygosity levels will be higher than if it has been inbred or backcrossed repeatedly.

Heterozygosity: 0.81%
Least Heterozygous Most Heterozygous

The ratio of reads mapped to Y-contigs to reads mapped to the whole Cannabis genome (Y-ratios) has been demonstrated to be strongly correlated with plant sex typing. This plot shows the distribution of Y-ratios for all samples in our database which were sequenced with the same method (panel or WGS) as this sample and where this sample falls in the distribution.

Y-Ratio Distribution: 0.0424
male female RSP11133

This chart represents the Illumina sequence coverage over the Bt/Bd allele. These are the three regions in the cannabis genome that impact THCA, CBDA, CBGA production. Coverage over the Active CBDAS gene is highly correlated with Type II and Type III plants as described by Etienne de Meijer. Coverage over the THCA gene is highly correlated with Type I and Type II plants but is anti-correlated with Type III plants. Type I plants require coverage over the inactive CBDA loci and no coverage over the Active CBDA gene. Lack of coverage over the Active CBDA and Active THCA allele are presumed to be Type IV plants (CBGA dominant). While deletions of entire THCAS and CBDAS genes are the most common Bt:Bd alleles observed, it is possible to have plants with these genes where functional expression of the enzyme is disrupted by deactivating point mutations (Kojoma et al. 2006).

Bt/Bd Allele Coverage

This chart represents the Illumina sequence coverage over the CBCA synthase gene.

CBCAS Coverage

Variants (THCAS, CBDAS, and CBCAS)

No variants to report

Variants (Select Genes of Interest)

PKSG-2a

UniProt

c.67T>A p.Phe23Ile missense variant moderate contig700 1945567

IGV: Start, Jump

A/T
NGS:
0.825
C90:
0.904
PKSG-2a

UniProt

c.31A>T p.Thr11Ser missense variant moderate contig700 1945603

IGV: Start, Jump

T/A
NGS:
0.763
C90:
0.876
PKSG-2b

UniProt

c.1152T>A p.Asn384Lys missense variant moderate contig700 1950486

IGV: Start, Jump

A/T
NGS:
0.715
C90:
0.895
PKSG-2b

UniProt

c.1132C>G p.Leu378Val missense variant moderate contig700 1950506

IGV: Start, Jump

G/C
NGS:
0.717
C90:
0.000
PKSG-2b

UniProt

c.1117A>G p.Ile373Val missense variant moderate contig700 1950521

IGV: Start, Jump

T/C
NGS:
0.807
C90:
0.981
PKSG-2b

UniProt

c.948T>G p.Asp316Glu missense variant moderate contig700 1950690

IGV: Start, Jump

A/C
NGS:
0.456
C90:
0.000
PKSG-2b

UniProt

c.945T>G p.Ser315Arg missense variant moderate contig700 1950693

IGV: Start, Jump

A/C
NGS:
0.454
C90:
0.000
PKSG-2b

UniProt

c.944G>A p.Ser315Asn missense variant moderate contig700 1950694

IGV: Start, Jump

C/T
NGS:
0.445
C90:
0.000
PKSG-2b

UniProt

c.934C>G p.His312Asp missense variant moderate contig700 1950704

IGV: Start, Jump

G/C
NGS:
0.410
C90:
0.000
PKSG-2b

UniProt

c.774G>A p.Met258Ile missense variant moderate contig700 1950864

IGV: Start, Jump

C/T
NGS:
0.366
C90:
0.947
PKSG-2b

UniProt

c.31A>T p.Thr11Ser missense variant moderate contig700 1951851

IGV: Start, Jump

T/A
NGS:
0.844
C90:
0.880
PKSG-2b

UniProt

c.-2_1dupATA start lost & conservative inframe insertion high contig700 1951880

IGV: Start, Jump

A/ATAT
NGS:
0.410
C90:
0.000
PKSG-4b

UniProt

c.496A>G p.Lys166Glu missense variant moderate contig700 2721177

IGV: Start, Jump

T/C
NGS:
0.651
C90:
0.684
PKSG-4b

UniProt

c.489delT p.Phe163fs frameshift variant high contig700 2721183

IGV: Start, Jump

CA/C
NGS:
0.792
C90:
0.761
PKSG-4b

UniProt

c.484_485insGGTTGAGGAAG p.Lys162fs frameshift variant high contig700 2721188

IGV: Start, Jump

T/TCTTCCTCAACC
NGS:
0.002
C90:
0.000
PKSG-4b

UniProt

c.485A>G p.Lys162Arg missense variant moderate contig700 2721188

IGV: Start, Jump

T/C
NGS:
0.643
C90:
0.301
PKSG-4b

UniProt

c.431T>G p.Val144Gly missense variant moderate contig700 2721242

IGV: Start, Jump

A/C
NGS:
0.575
C90:
0.679
PKSG-4b

UniProt

c.352_355delACAG p.Thr118fs frameshift variant high contig700 2721317

IGV: Start, Jump

CCTGT/C
NGS:
0.529
C90:
0.000
DXR-2

UniProt

c.1319T>C p.Ile440Thr missense variant moderate contig380 285250

IGV: Start, Jump

A/G
NGS:
0.480
C90:
0.000
aPT1

UniProt

c.629C>T p.Thr210Ile missense variant moderate contig121 2840237

IGV: Start, Jump

C/T
NGS:
0.561
C90:
0.598
HDS-2

UniProt

c.82_93delGTAACCGGAACT p.Val28_Thr31del conservative inframe deletion moderate contig95 1989748

IGV: Start, Jump

CGTAACCGGAACT/C
NGS:
0.805
C90:
0.000
HDS-2

UniProt

c.127T>G p.Ser43Ala missense variant moderate contig95 1989794

IGV: Start, Jump

T/G
NGS:
0.721
C90:
0.000

Nearest genetic relatives (All Samples)

0 0.075 0.150 0.225 0.300
clone distance sibling distance more distant
  1. 0.213 Cbot-2019-003 (RSP11131)
  2. 0.222 AVIDEKEL 2 0 (RSP11174)
  3. 0.226 Cbot-2019-004 (RSP11132)
  4. 0.256 CANNATONIC (RSP11349)
  5. 0.261 101st AIRBORN CBD (RSP11350)
  6. 0.266 Cbot-2019-002 (RSP11130)
  7. 0.267 Kush Hemp E1 (RSP11128)
  8. 0.268 Charlotte Dream (RSP11412)
  9. 0.278 Medxotic (RSP11410)
  10. 0.282 Miss X (RSP10999)
  11. 0.284 Queen Dream CBG (RSP11295)
  12. 0.291 Queen Dream (RSP11278)
  13. 0.292 JL Cross 6 (RSP11507)
  14. 0.292 13 Cherries (RSP11442)
  15. 0.295 Trump x Trump (RSP11466)
  16. 0.295 Ringo s Angel (RSP10085)
  17. 0.297 Wilburs Great Adventure (RSP11727)
  18. 0.298 Cherry Blossom (RSP11314)
  19. 0.299 High Amperage (RSP11726)
  20. 0.299 Cherry Blossom (RSP11331)

Nearest genetic relatives (Base Tree)

0 0.092 0.183 0.275 0.367
clone distance sibling distance more distant
  1. 0.231 Cbot-2019-004 (RSP11132)
  2. 0.279 Kush Hemp E1 (RSP11128)
  3. 0.315 Recon (RSP10755)
  4. 0.319 Blueberry Cheesecake (RSP10684)
  5. 0.322 Liberty Haze (RSP11000)
  6. 0.332 Durban Poison (RSP11014)
  7. 0.341 Queen Jesus (RSP10105)
  8. 0.345 Gold Cracker (RSP11048)
  9. 0.346 RKM-2018-020 (RSP11112)
  10. 0.348 UP Sunrise (RSP10989)
  11. 0.349 Hermaphrodite ResearchSample2 (RSP11050)
  12. 0.353 Cherry (RSP11142)
  13. 0.354 Pie Hoe (RSP11073)
  14. 0.356 RKM-2018-003 (RSP11094)
  15. 0.360 RKM-2018-019 (RSP11111)
  16. 0.361 Jiangji (RSP10653)
  17. 0.362 Cbot-2019-001 (RSP11129)
  18. 0.363 Cherry (RSP11143)
  19. 0.364 Cbot-2019-006 (RSP11134)
  20. 0.365 KYRG-11 (RSP11051)

Most genetically distant strains (All Samples)

0 0.125 0.250 0.375 0.500
clone distance sibling distance more distant
  1. 0.483 Cherry Blossom (RSP11318)
  2. 0.470 JL Tent 4 (RSP11491)
  3. 0.469 JL Cross 9 (RSP11510)
  4. 0.469 Cherry Blossom (RSP11326)
  5. 0.467 JL yellow (RSP11075)
  6. 0.464 JL Cross 2 (RSP11503)
  7. 0.464 JL Cross 15 (RSP11516)
  8. 0.464 JL Cross 22 (RSP11523)
  9. 0.463 JL 3rd Gen Mother (RSP11214)
  10. 0.462 JL X NSPM1 30 (RSP11476)
  11. 0.461 JL 3rd Gen Mother (RSP11197)
  12. 0.458 JL Cross 23 (RSP11524)
  13. 0.457 JL Tent 1 yellow stake (RSP11488)
  14. 0.454 JL Cross 19 (RSP11520)
  15. 0.451 Chem 91 (RSP11185)
  16. 0.447 Dave Pineapple (RSP11626)
  17. 0.445 Cherry Blossom (RSP11306)
  18. 0.444 JL x NSPM1 1 (RSP11478)
  19. 0.442 JL Cross 21 (RSP11522)
  20. 0.442 Cherry Blossom (RSP11323)

Most genetically distant strains (Base Tree)

0 0.117 0.233 0.350 0.467
clone distance sibling distance more distant
  1. 0.466 JL yellow (RSP11075)
  2. 0.423 RKM-2018-002 (RSP11093)
  3. 0.418 Skywalker OG (RSP10837)
  4. 0.417 RKM-2018-018 (RSP11110)
  5. 0.417 Hermaphrodite Research Sample1 (RSP11049)
  6. 0.413 Italian Kiss (RSP11034)
  7. 0.410 Monoica (RSP10241)
  8. 0.410 The Gift (RSP10988)
  9. 0.409 Black Beauty (RSP11035)
  10. 0.405 RKM-2018-023 (RSP11115)
  11. 0.405 RKM-2018-009 (RSP11100)
  12. 0.404 Sour Raspberry (RSP10551)
  13. 0.403 Feral (RSP10890)
  14. 0.402 Tygra (RSP10667)
  15. 0.401 RKM-2018-004 (RSP11096)
  16. 0.400 Lovrin (RSP10658)
  17. 0.399 Carmagnola (RSP11037)
  18. 0.398 Fedora 17 (RSP10661)
  19. 0.397 Ivory (RSP10668)
  20. 0.397 Kyrgyz Gold (RSP11054)

Nearest genetic relative in Phylos dataset

Phylos Strain SRR8347304
Overlapping SNPs:
123
Concordance:
87

Nearest genetic relative in Lynch dataset

Lynch Strain SRR3495250
Overlapping SNPs:
3
Concordance:
3

Blockchain Registration Information

Transaction ID
6a730f9bfdf2012f40fa6f202a4ece08037764e9f2633cf33c3715723bdc3422
Stamping Certificate
Download PDF (844.9 KB)
SHASUM Hash
d84ffd750f97c43adbc75da8f4c3ced744b7c299981deb36d4d083d299d31ee8
QR code for RSP11133

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