RKM-2018-024

RSP 11116

Grower: R-Kiem Seeds

General Information

Accession Date
October 21, 2018
Reported Plant Sex
Female

The strain rarity visualization shows how distant the strain is from the other cultivars in the Kannapedia database. The y-axis represents genetic distance, getting farther as you go up. The width of the visualization at any position along the y-axis shows how many strains there are in the database at that genetic distance. So, a common strain will have a more bottom-heavy shape, while uncommon and rare cultivars will have a visualization that is generally shifted towards the top.

Rarity: Rare
Most Distant Most Similar

Chemical Information

Cannabinoid and terpenoid information provided by the grower.

Cannabinoids

No information provided.

Terpenoids

No information provided.

Genetic Information

Plant Type
Type I

The bell curve in the heterozygosity visualization shows the distribution of heterozygosity levels for cannabis cultivars in the Kannapedia database. The green line shows where this particular strain fits within the distribution. Heterozygosity is associated with heterosis (aka hybrid vigor) but also leads to the production of more variable offspring. When plants have two genetically different parents, heterozygosity levels will be higher than if it has been inbred or backcrossed repeatedly.

Heterozygosity: 1.21%
Least Heterozygous Most Heterozygous

The ratio of reads mapped to Y-contigs to reads mapped to the whole Cannabis genome (Y-ratios) has been demonstrated to be strongly correlated with plant sex typing. This plot shows the distribution of Y-ratios for all samples in our database which were sequenced with the same method (panel or WGS) as this sample and where this sample falls in the distribution.

Y-Ratio Distribution: 0.0406
male female RSP11116

This chart represents the Illumina sequence coverage over the Bt/Bd allele. These are the three regions in the cannabis genome that impact THCA, CBDA, CBGA production. Coverage over the Active CBDAS gene is highly correlated with Type II and Type III plants as described by Etienne de Meijer. Coverage over the THCA gene is highly correlated with Type I and Type II plants but is anti-correlated with Type III plants. Type I plants require coverage over the inactive CBDA loci and no coverage over the Active CBDA gene. Lack of coverage over the Active CBDA and Active THCA allele are presumed to be Type IV plants (CBGA dominant). While deletions of entire THCAS and CBDAS genes are the most common Bt:Bd alleles observed, it is possible to have plants with these genes where functional expression of the enzyme is disrupted by deactivating point mutations (Kojoma et al. 2006).

Bt/Bd Allele Coverage

This chart represents the Illumina sequence coverage over the CBCA synthase gene.

CBCAS Coverage

Variants (THCAS, CBDAS, and CBCAS)

THCAS c.1229G>A p.Gly410Glu missense variant moderate contig741 4416599

IGV: Start, Jump

C/T
NGS:
0.039
C90:
0.000
THCAS c.373G>C p.Val125Leu missense variant moderate contig741 4417455

IGV: Start, Jump

C/G
NGS:
0.050
C90:
0.000

Variants (Select Genes of Interest)

PKSG-4a

UniProt

c.493G>A p.Gly165Ser missense variant moderate contig700 1937904

IGV: Start, Jump

G/A
NGS:
0.103
C90:
0.000
PKSG-2a

UniProt

c.1117A>G p.Ile373Val missense variant moderate contig700 1944273

IGV: Start, Jump

T/C
NGS:
0.101
C90:
0.967
PKSG-2a

UniProt

c.948T>G p.Asp316Glu missense variant moderate contig700 1944442

IGV: Start, Jump

A/C
NGS:
0.079
C90:
0.000
PKSG-2a

UniProt

c.945T>G p.Ser315Arg missense variant moderate contig700 1944445

IGV: Start, Jump

A/C
NGS:
0.079
C90:
0.000
PKSG-2a

UniProt

c.943_944insAGGA p.Ser315fs frameshift variant high contig700 1944446

IGV: Start, Jump

C/CTCCT
NGS:
0.002
C90:
0.000
PKSG-2a

UniProt

c.944G>A p.Ser315Asn missense variant moderate contig700 1944446

IGV: Start, Jump

C/T
NGS:
0.079
C90:
0.000
PKSG-2a

UniProt

c.934C>G p.His312Asp missense variant moderate contig700 1944456

IGV: Start, Jump

G/C
NGS:
0.070
C90:
0.000
PKSG-2a

UniProt

c.67T>A p.Phe23Ile missense variant moderate contig700 1945567

IGV: Start, Jump

A/T
NGS:
0.825
C90:
0.904
PKSG-2b

UniProt

c.1117A>G p.Ile373Val missense variant moderate contig700 1950521

IGV: Start, Jump

T/C
NGS:
0.807
C90:
0.981
PKSG-2b

UniProt

c.31A>T p.Thr11Ser missense variant moderate contig700 1951851

IGV: Start, Jump

T/A
NGS:
0.844
C90:
0.880
PKSG-4b

UniProt

c.544G>T p.Gly182Trp missense variant moderate contig700 2721129

IGV: Start, Jump

C/A
NGS:
0.055
C90:
0.000
PKSG-4b

UniProt

c.496A>G p.Lys166Glu missense variant moderate contig700 2721177

IGV: Start, Jump

T/C
NGS:
0.651
C90:
0.684
PKSG-4b

UniProt

c.489delT p.Phe163fs frameshift variant high contig700 2721183

IGV: Start, Jump

CA/C
NGS:
0.792
C90:
0.761
PKSG-4b

UniProt

c.485A>G p.Lys162Arg missense variant moderate contig700 2721188

IGV: Start, Jump

T/C
NGS:
0.643
C90:
0.301
PKSG-4b

UniProt

c.431T>G p.Val144Gly missense variant moderate contig700 2721242

IGV: Start, Jump

A/C
NGS:
0.575
C90:
0.679
PKSG-4b

UniProt

c.419A>G p.Asp140Gly missense variant moderate contig700 2721254

IGV: Start, Jump

T/C
NGS:
0.430
C90:
0.440
PKSG-4b

UniProt

c.352_355delACAG p.Thr118fs frameshift variant high contig700 2721317

IGV: Start, Jump

CCTGT/C
NGS:
0.529
C90:
0.000
PKSG-4b

UniProt

c.353_354insCC p.Gly119fs frameshift variant high contig700 2721319

IGV: Start, Jump

T/TGG
NGS:
0.175
C90:
0.000
PKSG-4b

UniProt

c.338G>A p.Gly113Glu missense variant moderate contig700 2721335

IGV: Start, Jump

C/T
NGS:
0.046
C90:
0.000
PKSG-4b

UniProt

c.323A>G p.Glu108Gly missense variant moderate contig700 2721350

IGV: Start, Jump

T/C
NGS:
0.469
C90:
0.000
aPT4

UniProt

c.775delT p.Tyr259fs frameshift variant high contig121 2831380

IGV: Start, Jump

AT/A
NGS:
0.224
C90:
0.000
aPT4

UniProt

c.1168T>C p.Tyr390His missense variant moderate contig121 2833503

IGV: Start, Jump

T/C
NGS:
0.546
C90:
0.000
aPT1

UniProt

c.406A>G p.Ile136Val missense variant moderate contig121 2839605

IGV: Start, Jump

A/G
NGS:
0.579
C90:
0.761
aPT1

UniProt

c.629C>T p.Thr210Ile missense variant moderate contig121 2840237

IGV: Start, Jump

C/T
NGS:
0.561
C90:
0.598
HDS-2

UniProt

c.82_93delGTAACCGGAACT p.Val28_Thr31del conservative inframe deletion moderate contig95 1989748

IGV: Start, Jump

CGTAACCGGAACT/C
NGS:
0.805
C90:
0.000
HDS-2

UniProt

c.127T>G p.Ser43Ala missense variant moderate contig95 1989794

IGV: Start, Jump

T/G
NGS:
0.721
C90:
0.000

Nearest genetic relatives (All Samples)

0 0.067 0.133 0.200 0.267
clone distance sibling distance more distant
  1. 0.222 Jacks Cleaner (RSP11347)
  2. 0.225 RKM-2018-023 (RSP11115)
  3. 0.245 OR (RSP10940)
  4. 0.245 UP Green Flash (RSP11259)
  5. 0.248 Tangerine Haze (RSP10995)
  6. 0.250 BLACK JACK (RSP11346)
  7. 0.253 T S A G E (RSP11351)
  8. 0.254 Hermaphrodite Research Sample1 (RSP11042)
  9. 0.257 Hermaphrodite Research Sample1 (RSP11049)
  10. 0.257 Italian Kiss (RSP10990)
  11. 0.258 Italian Kiss (RSP11034)
  12. 0.258 UP Sunrise (RSP10989)
  13. 0.259 Black Jack (RSP10603)
  14. 0.260 Gold Cracker (RSP11041)
  15. 0.261 UP The Straits (RSP11260)
  16. 0.261 RKM-2018-025 (RSP11117)
  17. 0.262 RKM-2018-016 (RSP11108)
  18. 0.262 Blue Dream (RSP11342)
  19. 0.265 EREZ USA (RSP11170)
  20. 0.266 Snoops Dream (RSP11003)

Nearest genetic relatives (Base Tree)

0 0.083 0.167 0.250 0.333
clone distance sibling distance more distant
  1. 0.223 RKM-2018-023 (RSP11115)
  2. 0.257 Hermaphrodite Research Sample1 (RSP11049)
  3. 0.258 Italian Kiss (RSP11034)
  4. 0.266 UP Sunrise (RSP10989)
  5. 0.271 RKM-2018-027 (RSP11119)
  6. 0.273 RKM-2018-018 (RSP11110)
  7. 0.273 Liberty Haze (RSP11000)
  8. 0.276 Gold Cracker (RSP11048)
  9. 0.277 Skunk 18 (RSP11038)
  10. 0.284 Blue Dream (RSP11033)
  11. 0.285 RKM-2018-009 (RSP11100)
  12. 0.291 RKM-2018-031 (RSP11123)
  13. 0.292 RKM-2018-006 (RSP11097)
  14. 0.295 RKM-2018-028 (RSP11120)
  15. 0.297 RKM-2018-003 (RSP11094)
  16. 0.298 Sour Raspberry (RSP10551)
  17. 0.299 RKM-2018-029 (RSP11121)
  18. 0.313 Blueberry Cheesecake (RSP10680)
  19. 0.313 RKM-2018-020 (RSP11112)
  20. 0.313 CST (RSP11002)

Most genetically distant strains (All Samples)

0 0.125 0.250 0.375 0.500
clone distance sibling distance more distant
  1. 0.485 Cherry Blossom (RSP11334)
  2. 0.483 Cherry Blossom (RSP11324)
  3. 0.482 Cherry Blossom (RSP11311)
  4. 0.472 Cherry Blossom (RSP11333)
  5. 0.459 Cherry Blossom (RSP11331)
  6. 0.458 Cherry Blossom (RSP11335)
  7. 0.457 Cherry Blossom (RSP11308)
  8. 0.454 Cherry Blossom (RSP11317)
  9. 0.453 Cherry Blossom (RSP11312)
  10. 0.449 Cherry Blossom (RSP11314)
  11. 0.448 Cherry Blossom (RSP11328)
  12. 0.447 Cherry Blossom (RSP11329)
  13. 0.445 Cherry Blossom (RSP11300)
  14. 0.443 Cherry Blossom CBG (RSP11303)
  15. 0.443 Cherry Blossom (RSP11323)
  16. 0.441 Cherry Blossom (RSP11321)
  17. 0.438 Cherry Blossom (RSP11319)
  18. 0.436 Cbot-2019-005 (RSP11133)
  19. 0.435 Cherry Blossom (RSP11302)
  20. 0.432 Cherry Blossom (RSP11298)

Most genetically distant strains (Base Tree)

0 0.117 0.233 0.350 0.467
clone distance sibling distance more distant
  1. 0.451 Cbot-2019-005 (RSP11133)
  2. 0.397 RKM-2018-034 (RSP11126)
  3. 0.396 Cherry (RSP11142)
  4. 0.392 Kush Hemp E1 (RSP11128)
  5. 0.382 Cbot-2019-004 (RSP11132)
  6. 0.374 Santhica27 (RSP11047)
  7. 0.368 Feral (RSP10890)
  8. 0.366 Ivory (RSP10668)
  9. 0.364 RKM-2018-026 (RSP11118)
  10. 0.363 Jiangji (RSP10653)
  11. 0.362 RKM-2018-019 (RSP11111)
  12. 0.362 Lovrin (RSP10658)
  13. 0.358 Futura 75 (RSP10664)
  14. 0.357 Kyrgyz Gold (RSP11054)
  15. 0.356 Carmagnola (RSP11037)
  16. 0.355 Fedora 17 (RSP10661)
  17. 0.354 The Gift (RSP10988)
  18. 0.354 Monoica (RSP10241)
  19. 0.354 Cherry (RSP11143)
  20. 0.354 Skywalker OG (RSP10837)

Nearest genetic relative in Phylos dataset

Phylos Strain SRR4448210
Overlapping SNPs:
92
Concordance:
69

Nearest genetic relative in Lynch dataset

Lynch Strain SRR3495250
Overlapping SNPs:
3
Concordance:
3

Blockchain Registration Information

Transaction ID
625eb3d16f0c2464435280e20338340df553d470061edaebe2ccde96bc0368a5
Stamping Certificate
Download PDF (860.3 KB)
SHASUM Hash
8284f3dd09398feeca1a968c893688ff2e8d3c1732687307ad4d368e8fde2211
QR code for RSP11116

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