JFG

RSP 12933

Grower: Zamir Punja

General Information

Sample Name
JFG - roots from flowering plant
Accession Date
September 27, 2023
Reported Plant Sex
Female
Microbiome
Krona Plot

The strain rarity visualization shows how distant the strain is from the other cultivars in the Kannapedia database. The y-axis represents genetic distance, getting farther as you go up. The width of the visualization at any position along the y-axis shows how many strains there are in the database at that genetic distance. So, a common strain will have a more bottom-heavy shape, while uncommon and rare cultivars will have a visualization that is generally shifted towards the top.

Rarity: Rare
Most Distant Most Similar

Chemical Information

Cannabinoid and terpenoid information provided by the grower.

Cannabinoids

No information provided.

Terpenoids

No information provided.

Genetic Information

Plant Type
Type I

The bell curve in the heterozygosity visualization shows the distribution of heterozygosity levels for cannabis cultivars in the Kannapedia database. The green line shows where this particular strain fits within the distribution. Heterozygosity is associated with heterosis (aka hybrid vigor) but also leads to the production of more variable offspring. When plants have two genetically different parents, heterozygosity levels will be higher than if it has been inbred or backcrossed repeatedly.

Heterozygosity: 1.18%
Least Heterozygous Most Heterozygous

The ratio of reads mapped to Y-contigs to reads mapped to the whole Cannabis genome (Y-ratios) has been demonstrated to be strongly correlated with plant sex typing. This plot shows the distribution of Y-ratios for all samples in our database which were sequenced with the same method (panel or WGS) as this sample and where this sample falls in the distribution.

Y-Ratio Distribution: 0.0279
male female RSP12933

This chart represents the Illumina sequence coverage over the Bt/Bd allele. These are the three regions in the cannabis genome that impact THCA, CBDA, CBGA production. Coverage over the Active CBDAS gene is highly correlated with Type II and Type III plants as described by Etienne de Meijer. Coverage over the THCA gene is highly correlated with Type I and Type II plants but is anti-correlated with Type III plants. Type I plants require coverage over the inactive CBDA loci and no coverage over the Active CBDA gene. Lack of coverage over the Active CBDA and Active THCA allele are presumed to be Type IV plants (CBGA dominant). While deletions of entire THCAS and CBDAS genes are the most common Bt:Bd alleles observed, it is possible to have plants with these genes where functional expression of the enzyme is disrupted by deactivating point mutations (Kojoma et al. 2006).

Bt/Bd Allele Coverage

This chart represents the Illumina sequence coverage over the CBCA synthase gene.

CBCAS Coverage

Variants (THCAS, CBDAS, and CBCAS)

Gene HGVS.c HGVS.p Annotation Annotation Impact Contig Contig Pos Ref/Alt Var Freq
THCAS c.749C>A p.Ala250Asp missense variant moderate contig741 4417079

IGV: Start, Jump

G/T
NGS:
0.127
C90:
0.632

Variants (Select Genes of Interest)

PHL-2 c.2783G>A p.Ser928Asn missense variant moderate contig2621 342826

IGV: Start, Jump

G/A
NGS:
0.107
C90:
0.890
PHL-2 c.2830A>G p.Asn944Asp missense variant moderate contig2621 342873

IGV: Start, Jump

A/G
NGS:
0.018
C90:
0.000
PHL-2 c.2903_2905dupGCA p.Ser968dup disruptive inframe insertion moderate contig2621 342939

IGV: Start, Jump

T/TGCA
NGS:
0.018
C90:
0.522
PKSG-4b

UniProt

c.489delT p.Phe163fs frameshift variant high contig700 2721183

IGV: Start, Jump

CA/C
NGS:
0.792
C90:
0.761
ELF3

UniProt

c.358G>A p.Gly120Arg missense variant moderate contig97 242064

IGV: Start, Jump

G/A
NGS:
0.099
C90:
0.493
ELF3

UniProt

c.772A>G p.Ser258Gly missense variant moderate contig97 242478

IGV: Start, Jump

A/G
NGS:
0.112
C90:
0.000
ELF3

UniProt

c.1630A>G p.Thr544Ala missense variant moderate contig97 244461

IGV: Start, Jump

A/G
NGS:
0.103
C90:
0.545
ELF3

UniProt

c.1803_1805delTCA p.His601del disruptive inframe deletion moderate contig97 244625

IGV: Start, Jump

ACAT/A
NGS:
0.092
C90:
0.000
ELF3

UniProt

c.2141C>G p.Pro714Arg missense variant moderate contig97 244972

IGV: Start, Jump

C/G
NGS:
0.088
C90:
0.335
FAD4

UniProt

c.121G>T p.Val41Phe missense variant moderate contig784 1690873

IGV: Start, Jump

G/T
NGS:
0.044
C90:
0.000
FAD4

UniProt

c.220C>G p.Arg74Gly missense variant moderate contig784 1690972

IGV: Start, Jump

C/G
NGS:
0.026
C90:
0.000
HDS-1

UniProt

c.56C>G p.Ala19Gly missense variant moderate contig1891 889336

IGV: Start, Jump

G/C
NGS:
0.125
C90:
0.943
HDS-1

UniProt

c.35G>A p.Cys12Tyr missense variant moderate contig1891 889357

IGV: Start, Jump

C/T
NGS:
0.094
C90:
0.646
EMF2

UniProt

c.1772A>G p.Gln591Arg missense variant moderate contig954 3059929

IGV: Start, Jump

A/G
NGS:
0.092
C90:
0.876
FLD

UniProt

c.125G>A p.Ser42Asn missense variant moderate contig1450 2047909

IGV: Start, Jump

C/T
NGS:
0.037
C90:
0.388
AAE1-3

UniProt

c.199A>G p.Asn67Asp missense variant moderate contig976 1083876

IGV: Start, Jump

T/C
NGS:
0.079
C90:
0.000
AAE1-3

UniProt

c.188A>G p.Asn63Ser missense variant moderate contig976 1083887

IGV: Start, Jump

T/C
NGS:
0.077
C90:
0.000
PIE1-1

UniProt

c.1222C>G p.Gln408Glu missense variant moderate contig1225 2281482

IGV: Start, Jump

C/G
NGS:
0.096
C90:
0.852
GGR

UniProt

c.704A>T p.His235Leu missense variant moderate contig2282 549696

IGV: Start, Jump

A/T
NGS:
0.121
C90:
0.000

Nearest genetic relatives (All Samples)

0 0.067 0.133 0.200 0.267
closely related moderately related distantly related
  1. 0.098 Powdered Donuts (RSP12939)
  2. 0.158 JFG (RSP12927)
  3. 0.203 Skywalker OG (RSP10837)
  4. 0.206 Old Family Purple (RSP12098)
  5. 0.212 SFVxTK (RSP11072)
  6. 0.220 501st OG (RSP11241)
  7. 0.223 Champelli Red 4 (RSP12804)
  8. 0.225 RKM-2018-012 (RSP11103)
  9. 0.225 East side OG (RSP12089)
  10. 0.227 Noetic OG (RSP11455)
  11. 0.228 BF (RSP12937)
  12. 0.229 Red Eye OG (RSP11190)
  13. 0.229 Purple Urkle (RSP12890)
  14. 0.229 Jilly bean (RSP11230)
  15. 0.233 BF (RSP12931)
  16. 0.238 unknown (RSP11432)
  17. 0.238 Muscovite (RSP12809)
  18. 0.239 RKM-2018-002 (RSP11093)
  19. 0.239 Eucalyptus (RSP12803)
  20. 0.240 Mexican Flan x Audios mother fu ker (RSP12906)

Most genetically distant strains (All Samples)

0 0.125 0.250 0.375 0.500
closely related moderately related distantly related
  1. 0.469 Cherry Blossom (RSP11317)
  2. 0.452 Cherry Blossom (RSP11298)
  3. 0.444 Cherry Blossom (RSP11311)
  4. 0.441 Northern Skunk (RSP11456)
  5. 0.440 Cherry Blossom (RSP11323)
  6. 0.436 Cherry Blossom (RSP11318)
  7. 0.434 Cherry Blossom (RSP11314)
  8. 0.434 Cherry Blossom (RSP11312)
  9. 0.433 Cherry Blossom (RSP11308)
  10. 0.433 80E (RSP11213)
  11. 0.432 Cherry Fog XL (RSP11458)
  12. 0.431 Cherry Blossom (RSP11328)
  13. 0.430 Cherry Blossom (RSP11335)
  14. 0.428 Cherry Blossom (RSP11306)
  15. 0.427 Cherry Blossom (RSP11330)
  16. 0.424 Cherry Blossom (RSP11309)
  17. 0.422 HM (RSP12940)
  18. 0.419 Unknown- Cherry Wine - 001 (RSP11268)
  19. 0.419 Unknown- Cherry Wine - 003 (RSP11270)
  20. 0.416 Cherry Blossom (RSP11329)

Nearest genetic relative in Phylos dataset

Phylos Strain SRR8348999
Overlapping SNPs:
83
Concordance:
54

Nearest genetic relative in Lynch dataset

Lynch Strain SRR3495250
Overlapping SNPs:
3
Concordance:
3

Blockchain Registration Information

Transaction ID
40bb81d8098593a2d3c6f1e6969d296cdd9fa31988cc50896ff360667310d972
Stamping Certificate
Download PDF (39.5 KB)
SHASUM Hash
b5efd98633d4b9e73a78eea7fbe26d0de39c427036743b49da79d6a3cefb794c
QR code for RSP12933

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