JFG

RSP 12927

Grower: Zamir Punja

General Information

Sample Name
JFG - roots from veg plant
Accession Date
September 27, 2023
Reported Plant Sex
Female
Microbiome
Krona Plot

The strain rarity visualization shows how distant the strain is from the other cultivars in the Kannapedia database. The y-axis represents genetic distance, getting farther as you go up. The width of the visualization at any position along the y-axis shows how many strains there are in the database at that genetic distance. So, a common strain will have a more bottom-heavy shape, while uncommon and rare cultivars will have a visualization that is generally shifted towards the top.

Rarity: Uncommon
Most Distant Most Similar

Chemical Information

Cannabinoid and terpenoid information provided by the grower.

Cannabinoids

No information provided.

Terpenoids

No information provided.

Genetic Information

Plant Type
Unknown

The bell curve in the heterozygosity visualization shows the distribution of heterozygosity levels for cannabis cultivars in the Kannapedia database. The green line shows where this particular strain fits within the distribution. Heterozygosity is associated with heterosis (aka hybrid vigor) but also leads to the production of more variable offspring. When plants have two genetically different parents, heterozygosity levels will be higher than if it has been inbred or backcrossed repeatedly.

Heterozygosity: 1.26%
Least Heterozygous Most Heterozygous

The ratio of reads mapped to Y-contigs to reads mapped to the whole Cannabis genome (Y-ratios) has been demonstrated to be strongly correlated with plant sex typing. This plot shows the distribution of Y-ratios for all samples in our database which were sequenced with the same method (panel or WGS) as this sample and where this sample falls in the distribution.

Y-Ratio Distribution: 0.0266
male female RSP12927

This chart represents the Illumina sequence coverage over the Bt/Bd allele. These are the three regions in the cannabis genome that impact THCA, CBDA, CBGA production. Coverage over the Active CBDAS gene is highly correlated with Type II and Type III plants as described by Etienne de Meijer. Coverage over the THCA gene is highly correlated with Type I and Type II plants but is anti-correlated with Type III plants. Type I plants require coverage over the inactive CBDA loci and no coverage over the Active CBDA gene. Lack of coverage over the Active CBDA and Active THCA allele are presumed to be Type IV plants (CBGA dominant). While deletions of entire THCAS and CBDAS genes are the most common Bt:Bd alleles observed, it is possible to have plants with these genes where functional expression of the enzyme is disrupted by deactivating point mutations (Kojoma et al. 2006).

Bt/Bd Allele Coverage

This chart represents the Illumina sequence coverage over the CBCA synthase gene.

CBCAS Coverage

Variants (THCAS, CBDAS, and CBCAS)

No variants to report

Variants (Select Genes of Interest)

PHL-2 c.455A>C p.Asp152Ala missense variant moderate contig2621 339191

IGV: Start, Jump

A/C
NGS:
0.018
C90:
0.507
PHL-2 c.2830A>G p.Asn944Asp missense variant moderate contig2621 342873

IGV: Start, Jump

A/G
NGS:
0.018
C90:
0.000
PHL-2 c.3467A>G p.Gln1156Arg missense variant moderate contig2621 343510

IGV: Start, Jump

A/G
NGS:
0.064
C90:
0.713
PKSG-4b

UniProt

c.353_354insCC p.Gly119fs frameshift variant high contig700 2721319

IGV: Start, Jump

T/TGG
NGS:
0.175
C90:
0.000
PKSG-4b

UniProt

c.206T>C p.Leu69Ser missense variant moderate contig700 2724229

IGV: Start, Jump

A/G
NGS:
0.044
C90:
0.000
FAD2-2

UniProt

c.161T>A p.Leu54His missense variant moderate contig83 1803208

IGV: Start, Jump

A/T
NGS:
0.156
C90:
0.000
FAD2-2

UniProt

c.154G>A p.Val52Ile missense variant moderate contig83 1803215

IGV: Start, Jump

C/T
NGS:
0.022
C90:
0.000
FAD2-2

UniProt

c.62C>T p.Pro21Leu missense variant moderate contig83 1803307

IGV: Start, Jump

G/A
NGS:
0.042
C90:
0.000
ELF3

UniProt

c.1230-2_1230-1delAG splice acceptor variant & intron variant high contig97 243676

IGV: Start, Jump

TAG/T
NGS:
0.088
C90:
0.450
ELF3

UniProt

c.1466G>A p.Ser489Asn missense variant moderate contig97 244297

IGV: Start, Jump

G/A
NGS:
0.123
C90:
0.000
ELF3

UniProt

c.1966C>G p.Pro656Ala missense variant moderate contig97 244797

IGV: Start, Jump

C/G
NGS:
0.123
C90:
0.632
ELF3

UniProt

c.2141C>G p.Pro714Arg missense variant moderate contig97 244972

IGV: Start, Jump

C/G
NGS:
0.088
C90:
0.335
aPT4

UniProt

c.35A>C p.Gln12Pro missense variant moderate contig121 2828691

IGV: Start, Jump

A/C
NGS:
0.075
C90:
0.000
HDS-2

UniProt

c.82_93delGTAACCGGAACT p.Val28_Thr31del conservative inframe deletion moderate contig95 1989748

IGV: Start, Jump

CGTAACCGGAACT/C
NGS:
0.805
C90:
0.000
HDS-2

UniProt

c.127T>G p.Ser43Ala missense variant moderate contig95 1989794

IGV: Start, Jump

T/G
NGS:
0.721
C90:
0.000
Edestin

UniProt

c.16T>C p.Ser6Pro missense variant moderate contig850 3065274

IGV: Start, Jump

A/G
NGS:
0.059
C90:
0.000
HDS-1

UniProt

c.1378G>A p.Val460Ile missense variant moderate contig1891 886370

IGV: Start, Jump

C/T
NGS:
0.167
C90:
0.000
HDS-1

UniProt

c.136G>A p.Val46Ile missense variant moderate contig1891 889256

IGV: Start, Jump

C/T
NGS:
0.088
C90:
0.593
HDS-1

UniProt

c.56C>G p.Ala19Gly missense variant moderate contig1891 889336

IGV: Start, Jump

G/C
NGS:
0.125
C90:
0.943
HDS-1

UniProt

c.35G>A p.Cys12Tyr missense variant moderate contig1891 889357

IGV: Start, Jump

C/T
NGS:
0.094
C90:
0.646
EMF2

UniProt

c.1772A>G p.Gln591Arg missense variant moderate contig954 3059929

IGV: Start, Jump

A/G
NGS:
0.092
C90:
0.876
FLD

UniProt

c.2981T>C p.Met994Thr missense variant moderate contig1450 2044012

IGV: Start, Jump

A/G
NGS:
0.064
C90:
0.478
FLD

UniProt

c.2686G>A p.Ala896Thr missense variant moderate contig1450 2044848

IGV: Start, Jump

C/T
NGS:
0.035
C90:
0.225
GGR

UniProt

c.704A>T p.His235Leu missense variant moderate contig2282 549696

IGV: Start, Jump

A/T
NGS:
0.121
C90:
0.000
PKSB-3

UniProt

c.1850_1852dupTGA p.Met617dup disruptive inframe insertion moderate contig93 3339945

IGV: Start, Jump

A/AGAT
NGS:
0.031
C90:
0.000

Nearest genetic relatives (All Samples)

0 0.058 0.117 0.175 0.233
clone distance sibling distance more distant
  1. 0.092 Powdered Donuts (RSP12939)
  2. 0.096 JFG (RSP12933)
  3. 0.177 Old Family Purple (RSP12098)
  4. 0.195 Skywalker OG (RSP10837)
  5. 0.196 SFVxTK (RSP11072)
  6. 0.196 BF (RSP12931)
  7. 0.199 501st OG (RSP11241)
  8. 0.200 Champelli Red 4 (RSP12804)
  9. 0.208 Absolute OG (RSP11455)
  10. 0.209 Jilly bean (RSP11230)
  11. 0.210 East side OG (RSP12089)
  12. 0.217 unknown (RSP11432)
  13. 0.217 Red Eye OG (RSP11190)
  14. 0.217 PG (RSP12934)
  15. 0.218 Eucalyptus (RSP12803)
  16. 0.219 RKM-2018-012 (RSP11103)
  17. 0.222 PG (RSP12928)
  18. 0.224 BF (RSP12937)
  19. 0.225 RKM-2018-002 (RSP11093)
  20. 0.225 Purple Urkle (RSP12890)

Nearest genetic relatives (Base Tree)

0 0.083 0.167 0.250 0.333
clone distance sibling distance more distant
  1. 0.201 Skywalker OG (RSP10837)
  2. 0.237 RKM-2018-002 (RSP11093)
  3. 0.248 Hermaphrodite ResearchSample2 (RSP11050)
  4. 0.253 Pie Hoe (RSP11073)
  5. 0.253 Liberty Haze (RSP11000)
  6. 0.261 Gold Cracker (RSP11048)
  7. 0.264 RKM-2018-034 (RSP11126)
  8. 0.265 RKM-2018-004 (RSP11096)
  9. 0.265 Durban Poison (RSP11014)
  10. 0.267 The Gift (RSP10988)
  11. 0.271 RKM-2018-032 (RSP11124)
  12. 0.271 RKM-2018-033 (RSP11125)
  13. 0.274 RKM-2018-020 (RSP11112)
  14. 0.276 RKM-2018-026 (RSP11118)
  15. 0.277 Blueberry Cheesecake (RSP10684)
  16. 0.293 Blueberry Cheesecake (RSP10680)
  17. 0.294 Kimbo Slice (RSP10997)
  18. 0.295 Hermaphrodite Research Sample1 (RSP11049)
  19. 0.301 Sour Raspberry (RSP10551)
  20. 0.303 Skunk 18 (RSP11038)

Most genetically distant strains (All Samples)

0 0.117 0.233 0.350 0.467
clone distance sibling distance more distant
  1. 0.453 Cherry Blossom (RSP11317)
  2. 0.447 Northern Skunk (RSP11456)
  3. 0.437 Cherry Blossom (RSP11298)
  4. 0.436 80E (RSP11213)
  5. 0.434 Cherry Blossom (RSP11323)
  6. 0.432 Cherry Blossom (RSP11311)
  7. 0.428 Cherry Blossom (RSP11328)
  8. 0.420 Cherry Blossom (RSP11318)
  9. 0.418 Cherry Blossom (RSP11309)
  10. 0.417 Cherry Fog XL (RSP11458)
  11. 0.415 Cherry Blossom (RSP11312)
  12. 0.415 Cherry Blossom (RSP11330)
  13. 0.415 Cherry Blossom (RSP11308)
  14. 0.413 Cherry Blossom (RSP11314)
  15. 0.412 80E (RSP11211)
  16. 0.411 Cherry Blossom (RSP11306)
  17. 0.411 Unknown- Cherry Wine - 001 (RSP11268)
  18. 0.410 Cherry Blossom (RSP11335)
  19. 0.410 Cherry Blossom (RSP11324)
  20. 0.408 Cherry Blossom (RSP11334)

Most genetically distant strains (Base Tree)

0 0.108 0.217 0.325 0.433
clone distance sibling distance more distant
  1. 0.402 Cbot-2019-005 (RSP11133)
  2. 0.386 Cherry (RSP11142)
  3. 0.383 Carmagnola (RSP11037)
  4. 0.379 Monoica (RSP10241)
  5. 0.377 Futura 75 (RSP10664)
  6. 0.374 RKM-2018-022 (RSP11114)
  7. 0.374 Feral (RSP10890)
  8. 0.372 Cherry (RSP11143)
  9. 0.372 JL yellow (RSP11075)
  10. 0.366 Fedora 17 (RSP10661)
  11. 0.361 KYRG-11 (RSP11051)
  12. 0.360 USO 31 (RSP10981)
  13. 0.360 Black Beauty (RSP11035)
  14. 0.359 RKM-2018-019 (RSP11111)
  15. 0.359 Santhica27 (RSP11047)
  16. 0.358 Lovrin (RSP10658)
  17. 0.355 Tisza (RSP11044)
  18. 0.355 Kyrgyz Gold (RSP11054)
  19. 0.355 Ivory (RSP10668)
  20. 0.353 Jiangji (RSP10653)

Nearest genetic relative in Phylos dataset

Phylos Strain SRR8348999
Overlapping SNPs:
95
Concordance:
65

Nearest genetic relative in Lynch dataset

Lynch Strain SRR3495250
Overlapping SNPs:
3
Concordance:
3

Blockchain Registration Information

Transaction ID
09b893b90e90872383a89693c0e94c22b25ffabf7f096e89b1627e7aa739861d
Stamping Certificate
Download PDF (39.5 KB)
SHASUM Hash
7208da704ad7203d12676affe21e8339f327c6c5a733361012500b06ad8ce9a5
QR code for RSP12927

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