CST

RSP 11002

Grower: DigiPath Labs

General Information

Sample Name
F20170120-01-12
Accession Date
August 28, 2017
Reported Plant Sex
Female

The strain rarity visualization shows how distant the strain is from the other cultivars in the Kannapedia database. The y-axis represents genetic distance, getting farther as you go up. The width of the visualization at any position along the y-axis shows how many strains there are in the database at that genetic distance. So, a common strain will have a more bottom-heavy shape, while uncommon and rare cultivars will have a visualization that is generally shifted towards the top.

Rarity: Uncommon
Most Distant Most Similar

Chemical Information

Cannabinoid and terpenoid information provided by the grower.

Cannabinoids

THC + THCA
14.3%
CBD + CBDA
9.678%
THCV + THCVA
0.014%
CBC + CBCA
0.023%
CBG + CBGA
0.252%
CBN + CBNA
0.065%

Terpenoids

α-Bisabolol
0.%
Borneol
n/a
Camphene
0.005%
Carene
0.038%
Caryophyllene oxide
0.%
β-Caryophyllene
0.044%
Fenchol
n/a
Geraniol
0.%
α-Humulene
0.06%
Limonene
0.047%
Linalool
0.02%
Myrcene
0.229%
α-Phellandrene
n/a
Terpinolene
0.215%
α-Terpineol
n/a
α-Terpinene
0.021%
γ-Terpinene
0.312%
Total Nerolidol
0.004%
Total Ocimene
0.089%
α-Pinene
0.308%
β-Pinene
0.178%

Genetic Information

Plant Type
Type II

The bell curve in the heterozygosity visualization shows the distribution of heterozygosity levels for cannabis cultivars in the Kannapedia database. The green line shows where this particular strain fits within the distribution. Heterozygosity is associated with heterosis (aka hybrid vigor) but also leads to the production of more variable offspring. When plants have two genetically different parents, heterozygosity levels will be higher than if it has been inbred or backcrossed repeatedly.

Heterozygosity: 0.72%
Least Heterozygous Most Heterozygous

The ratio of reads mapped to Y-contigs to reads mapped to the whole Cannabis genome (Y-ratios) has been demonstrated to be strongly correlated with plant sex typing. This plot shows the distribution of Y-ratios for all samples in our database which were sequenced with the same method (panel or WGS) as this sample and where this sample falls in the distribution.

Y-Ratio Distribution: 0.0493
male female RSP11002

This chart represents the Illumina sequence coverage over the Bt/Bd allele. These are the three regions in the cannabis genome that impact THCA, CBDA, CBGA production. Coverage over the Active CBDAS gene is highly correlated with Type II and Type III plants as described by Etienne de Meijer. Coverage over the THCA gene is highly correlated with Type I and Type II plants but is anti-correlated with Type III plants. Type I plants require coverage over the inactive CBDA loci and no coverage over the Active CBDA gene. Lack of coverage over the Active CBDA and Active THCA allele are presumed to be Type IV plants (CBGA dominant). While deletions of entire THCAS and CBDAS genes are the most common Bt:Bd alleles observed, it is possible to have plants with these genes where functional expression of the enzyme is disrupted by deactivating point mutations (Kojoma et al. 2006).

Bt/Bd Allele Coverage

This chart represents the Illumina sequence coverage over the CBCA synthase gene.

CBCAS Coverage

Variants (THCAS, CBDAS, and CBCAS)

Gene HGVS.c HGVS.p Annotation Annotation Impact Contig Contig Pos Ref/Alt Var Freq
THCAS c.187A>C p.Ile63Leu missense variant moderate contig741 4417641

IGV: Start, Jump

T/G
NGS:
0.059
C90:
0.679

Variants (Select Genes of Interest)

PKSG-2a

UniProt

c.67T>A p.Phe23Ile missense variant moderate contig700 1945567

IGV: Start, Jump

A/T
NGS:
0.825
C90:
0.904
PKSG-2a

UniProt

c.31A>T p.Thr11Ser missense variant moderate contig700 1945603

IGV: Start, Jump

T/A
NGS:
0.763
C90:
0.876
PKSG-4b

UniProt

c.496A>G p.Lys166Glu missense variant moderate contig700 2721177

IGV: Start, Jump

T/C
NGS:
0.651
C90:
0.684
PKSG-4b

UniProt

c.489delT p.Phe163fs frameshift variant high contig700 2721183

IGV: Start, Jump

CA/C
NGS:
0.792
C90:
0.761
PKSG-4b

UniProt

c.485A>G p.Lys162Arg missense variant moderate contig700 2721188

IGV: Start, Jump

T/C
NGS:
0.643
C90:
0.301
PKSG-4b

UniProt

c.431T>G p.Val144Gly missense variant moderate contig700 2721242

IGV: Start, Jump

A/C
NGS:
0.575
C90:
0.679
PKSG-4b

UniProt

c.419A>G p.Asp140Gly missense variant moderate contig700 2721254

IGV: Start, Jump

T/C
NGS:
0.430
C90:
0.440
aPT1

UniProt

c.406A>G p.Ile136Val missense variant moderate contig121 2839605

IGV: Start, Jump

A/G
NGS:
0.579
C90:
0.761

Nearest genetic relatives (All Samples)

0 0.058 0.117 0.175 0.233
clone distance sibling distance more distant
  1. 0.010 Sour Tsunami (RSP11187)
  2. 0.187 Blue Dream (RSP11008)
  3. 0.190 Blue Dream (RSP11010)
  4. 0.193 Blue Dream (RSP11007)
  5. 0.193 Durban Poison #1 (RSP11013)
  6. 0.196 Durban Poison #1 (RSP10996)
  7. 0.197 Blue Dream (RSP11342)
  8. 0.200 Snoops Dream (RSP11003)
  9. 0.200 Blue Dream (RSP11004)
  10. 0.203 Snoops Dream (RSP11031)
  11. 0.204 Blue Dream (RSP11033)
  12. 0.207 Electra (RSP11366)
  13. 0.208 Blue Dream (RSP11006)
  14. 0.208 Lift (RSP11378)
  15. 0.209 Super Blue Dream (RSP11011)
  16. 0.210 Blue Dream (RSP11009)
  17. 0.211 Durban Poison (RSP11014)
  18. 0.212 Blue Dream (RSP11032)
  19. 0.212 Black Jack (RSP10603)
  20. 0.213 Blue Dream (RSP11005)

Nearest genetic relatives (Base Tree)

0 0.075 0.150 0.225 0.300
clone distance sibling distance more distant
  1. 0.200 Durban Poison (RSP11014)
  2. 0.217 Blue Dream (RSP11033)
  3. 0.225 Blueberry Cheesecake (RSP10684)
  4. 0.228 Golden Goat 2 (RSP10991)
  5. 0.234 Liberty Haze (RSP11000)
  6. 0.240 Italian Kiss (RSP11034)
  7. 0.241 RKM-2018-003 (RSP11094)
  8. 0.243 RKM-2018-027 (RSP11119)
  9. 0.243 RKM-2018-031 (RSP11123)
  10. 0.244 UP Sunrise (RSP10989)
  11. 0.251 Gold Cracker (RSP11048)
  12. 0.257 RKM-2018-023 (RSP11115)
  13. 0.258 Recon (RSP10755)
  14. 0.262 Blueberry Cheesecake (RSP10672)
  15. 0.265 RKM-2018-029 (RSP11121)
  16. 0.268 Queen Jesus (RSP10105)
  17. 0.271 RKM-2018-020 (RSP11112)
  18. 0.280 Hermaphrodite Research Sample1 (RSP11049)
  19. 0.282 Tisza (RSP10659)
  20. 0.284 Skunk#18 (RSP11038)

Most genetically distant strains (All Samples)

0 0.117 0.233 0.350 0.467
clone distance sibling distance more distant
  1. 0.435 Cherry Blossom (RSP11323)
  2. 0.431 Cherry Blossom (RSP11318)
  3. 0.427 Cherry Blossom (RSP11328)
  4. 0.421 Cherry Blossom (RSP11333)
  5. 0.420 Cherry Blossom (RSP11314)
  6. 0.412 Cherry Blossom (RSP11311)
  7. 0.411 Cherry Blossom (RSP11317)
  8. 0.408 Red Eye OG (RSP11190)
  9. 0.406 80E (RSP11213)
  10. 0.402 Cherry Blossom (RSP11312)
  11. 0.402 CS (RSP11208)
  12. 0.392 Cherry Blossom (RSP11309)
  13. 0.388 Cherry Blossom (RSP11335)
  14. 0.388 80E (RSP11212)
  15. 0.386 Cherry Blossom (RSP11308)
  16. 0.384 Cherry Blossom (RSP11334)
  17. 0.383 Cherry Blossom (RSP11302)
  18. 0.382 Cherry Blossom (RSP11324)
  19. 0.379 East side OG (RSP12089)
  20. 0.378 Carmaleonte (RSP11207)

Most genetically distant strains (Base Tree)

0 0.100 0.200 0.300 0.400
clone distance sibling distance more distant
  1. 0.375 Cbot-2019-005 (RSP11133)
  2. 0.372 RKM-2018-034 (RSP11126)
  3. 0.369 RKM-2018-026 (RSP11118)
  4. 0.359 Skywalker OG (RSP10837)
  5. 0.359 Santhica27 (RSP11047)
  6. 0.358 Carmagnola (RSP11037)
  7. 0.355 Kush Hemp E1 (RSP11128)
  8. 0.354 Cbot-2019-001 (RSP11129)
  9. 0.354 Monoica (RSP10241)
  10. 0.349 Fedora 17 (RSP10661)
  11. 0.346 Feral (RSP10890)
  12. 0.342 Carmagnola (RSP10979)
  13. 0.341 Ivory (RSP10668)
  14. 0.339 Futura 75 (RSP10664)
  15. 0.338 RKM-2018-019 (RSP11111)
  16. 0.335 USO 31 (RSP10981)
  17. 0.334 KYRG-11 (RSP11051)
  18. 0.334 Pie Hoe (RSP11073)
  19. 0.330 The Gift (RSP10988)
  20. 0.330 RKM-2018-002 (RSP11093)

Nearest genetic relative in Phylos dataset

Phylos Strain SRR8347163
Overlapping SNPs:
79
Concordance:
54

Nearest genetic relative in Lynch dataset

Lynch Strain SRR3495180
Overlapping SNPs:
5
Concordance:
5

Blockchain Registration Information

Transaction ID
3af7f4c0e763b00d28ae9fd08ece580da83f0342d5ed20888d4778d4e5a2a0e7
Stamping Certificate
Download PDF (858.1 KB)
SHASUM Hash
e4823b31bb90d2b282d511f1e22507f426d3b921dc20aecb92385ff7f6ab1d4e
QR code for RSP11002

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