RKM-2018-030

RSP 11122

Grower: R-Kiem Seeds

General Information

Accession Date
October 21, 2018
Reported Plant Sex
Female

The strain rarity visualization shows how distant the strain is from the other cultivars in the Kannapedia database. The y-axis represents genetic distance, getting farther as you go up. The width of the visualization at any position along the y-axis shows how many strains there are in the database at that genetic distance. So, a common strain will have a more bottom-heavy shape, while uncommon and rare cultivars will have a visualization that is generally shifted towards the top.

Rarity: Rare
Most Distant Most Similar

Chemical Information

Cannabinoid and terpenoid information provided by the grower.

Cannabinoids

No information provided.

Terpenoids

No information provided.

Genetic Information

Plant Type
Type I

The bell curve in the heterozygosity visualization shows the distribution of heterozygosity levels for cannabis cultivars in the Kannapedia database. The green line shows where this particular strain fits within the distribution. Heterozygosity is associated with heterosis (aka hybrid vigor) but also leads to the production of more variable offspring. When plants have two genetically different parents, heterozygosity levels will be higher than if it has been inbred or backcrossed repeatedly.

Heterozygosity: 1.41%
Least Heterozygous Most Heterozygous

The ratio of reads mapped to Y-contigs to reads mapped to the whole Cannabis genome (Y-ratios) has been demonstrated to be strongly correlated with plant sex typing. This plot shows the distribution of Y-ratios for all samples in our database which were sequenced with the same method (panel or WGS) as this sample and where this sample falls in the distribution.

Y-Ratio Distribution: 0.0411
male female RSP11122

This chart represents the Illumina sequence coverage over the Bt/Bd allele. These are the three regions in the cannabis genome that impact THCA, CBDA, CBGA production. Coverage over the Active CBDAS gene is highly correlated with Type II and Type III plants as described by Etienne de Meijer. Coverage over the THCA gene is highly correlated with Type I and Type II plants but is anti-correlated with Type III plants. Type I plants require coverage over the inactive CBDA loci and no coverage over the Active CBDA gene. Lack of coverage over the Active CBDA and Active THCA allele are presumed to be Type IV plants (CBGA dominant). While deletions of entire THCAS and CBDAS genes are the most common Bt:Bd alleles observed, it is possible to have plants with these genes where functional expression of the enzyme is disrupted by deactivating point mutations (Kojoma et al. 2006).

Bt/Bd Allele Coverage

This chart represents the Illumina sequence coverage over the CBCA synthase gene.

CBCAS Coverage

Variants (THCAS, CBDAS, and CBCAS)

Gene HGVS.c HGVS.p Annotation Annotation Impact Contig Contig Pos Ref/Alt Var Freq
THCAS c.998C>G p.Pro333Arg missense variant moderate contig741 4416830

IGV: Start, Jump

G/C
NGS:
0.182
C90:
0.000

Variants (Select Genes of Interest)

PKSG-4a

UniProt

c.617A>G p.Tyr206Cys missense variant moderate contig700 1938028

IGV: Start, Jump

A/G
NGS:
0.075
C90:
0.000
PKSG-4a

UniProt

c.626_628delATA p.Asn209del disruptive inframe deletion moderate contig700 1938032

IGV: Start, Jump

CAAT/C
NGS:
0.075
C90:
0.000
PKSG-4a

UniProt

c.1191_1193delTTA p.Tyr398del disruptive inframe deletion moderate contig700 1938600

IGV: Start, Jump

AATT/A
NGS:
0.167
C90:
0.000
PKSG-2a

UniProt

c.1136G>A p.Arg379His missense variant moderate contig700 1944254

IGV: Start, Jump

C/T
NGS:
0.020
C90:
0.000
PKSG-2a

UniProt

c.948T>G p.Asp316Glu missense variant moderate contig700 1944442

IGV: Start, Jump

A/C
NGS:
0.079
C90:
0.000
PKSG-2a

UniProt

c.945T>G p.Ser315Arg missense variant moderate contig700 1944445

IGV: Start, Jump

A/C
NGS:
0.079
C90:
0.000
PKSG-2a

UniProt

c.944G>A p.Ser315Asn missense variant moderate contig700 1944446

IGV: Start, Jump

C/T
NGS:
0.079
C90:
0.000
PKSG-2a

UniProt

c.938_939insGATG p.Lys314fs frameshift variant high contig700 1944451

IGV: Start, Jump

T/TCATC
NGS:
0.002
C90:
0.000
PKSG-2a

UniProt

c.934C>G p.His312Asp missense variant moderate contig700 1944456

IGV: Start, Jump

G/C
NGS:
0.070
C90:
0.000
PKSG-2a

UniProt

c.67T>A p.Phe23Ile missense variant moderate contig700 1945567

IGV: Start, Jump

A/T
NGS:
0.825
C90:
0.904
PKSG-2a

UniProt

c.31A>T p.Thr11Ser missense variant moderate contig700 1945603

IGV: Start, Jump

T/A
NGS:
0.763
C90:
0.876
PKSG-2b

UniProt

c.1132C>G p.Leu378Val missense variant moderate contig700 1950506

IGV: Start, Jump

G/C
NGS:
0.717
C90:
0.000
PKSG-2b

UniProt

c.1117A>G p.Ile373Val missense variant moderate contig700 1950521

IGV: Start, Jump

T/C
NGS:
0.807
C90:
0.981
PKSG-2b

UniProt

c.948T>G p.Asp316Glu missense variant moderate contig700 1950690

IGV: Start, Jump

A/C
NGS:
0.456
C90:
0.000
PKSG-2b

UniProt

c.945T>G p.Ser315Arg missense variant moderate contig700 1950693

IGV: Start, Jump

A/C
NGS:
0.454
C90:
0.000
PKSG-2b

UniProt

c.944G>A p.Ser315Asn missense variant moderate contig700 1950694

IGV: Start, Jump

C/T
NGS:
0.445
C90:
0.000
PKSG-2b

UniProt

c.934C>G p.His312Asp missense variant moderate contig700 1950704

IGV: Start, Jump

G/C
NGS:
0.410
C90:
0.000
PKSG-2b

UniProt

c.67A>T p.Ile23Phe missense variant moderate contig700 1951815

IGV: Start, Jump

T/A
NGS:
0.033
C90:
0.325
PKSG-2b

UniProt

c.31A>T p.Thr11Ser missense variant moderate contig700 1951851

IGV: Start, Jump

T/A
NGS:
0.844
C90:
0.880
PKSG-2b

UniProt

c.-2_1dupATA start lost & conservative inframe insertion high contig700 1951880

IGV: Start, Jump

A/ATAT
NGS:
0.410
C90:
0.000
DXR-2

UniProt

c.1319T>C p.Ile440Thr missense variant moderate contig380 285250

IGV: Start, Jump

A/G
NGS:
0.480
C90:
0.000
aPT1

UniProt

c.406A>G p.Ile136Val missense variant moderate contig121 2839605

IGV: Start, Jump

A/G
NGS:
0.579
C90:
0.761
HDS-2

UniProt

c.82_93delGTAACCGGAACT p.Val28_Thr31del conservative inframe deletion moderate contig95 1989748

IGV: Start, Jump

CGTAACCGGAACT/C
NGS:
0.805
C90:
0.000
HDS-2

UniProt

c.127T>G p.Ser43Ala missense variant moderate contig95 1989794

IGV: Start, Jump

T/G
NGS:
0.721
C90:
0.000

Nearest genetic relatives (All Samples)

0 0.067 0.133 0.200 0.267
clone distance sibling distance more distant
  1. 0.187 Cheese (RSP10460)
  2. 0.188 RKM-2018-028 (RSP11120)
  3. 0.211 RKM-2018-003 (RSP11094)
  4. 0.226 Saint Jack (RSP11179)
  5. 0.228 RKM-2018-007 (RSP11098)
  6. 0.230 Tangerine Haze (RSP10995)
  7. 0.231 Black Jack (RSP10603)
  8. 0.232 Sour D (RSP11343)
  9. 0.232 CST (RSP11002)
  10. 0.233 Serious Happiness (RSP10763)
  11. 0.233 Cbot-2019-006 (RSP11134)
  12. 0.233 BLACK JACK (RSP11346)
  13. 0.234 Italian Kiss (RSP10990)
  14. 0.235 Alaska USA (RSP11171)
  15. 0.235 Durban Poison 1 (RSP10996)
  16. 0.235 Erez (RSP10942)
  17. 0.237 T S A G E (RSP11351)
  18. 0.240 Domnesia (RSP11184)
  19. 0.240 Italian Kiss (RSP11034)
  20. 0.241 RKM-2018-010 (RSP11101)

Nearest genetic relatives (Base Tree)

0 0.067 0.133 0.200 0.267
clone distance sibling distance more distant
  1. 0.190 RKM-2018-028 (RSP11120)
  2. 0.219 RKM-2018-003 (RSP11094)
  3. 0.227 CST (RSP11002)
  4. 0.234 Cbot-2019-006 (RSP11134)
  5. 0.235 RKM-2018-022 (RSP11114)
  6. 0.241 Sour Raspberry (RSP10551)
  7. 0.243 Italian Kiss (RSP11034)
  8. 0.244 RKM-2018-023 (RSP11115)
  9. 0.245 Gold Cracker (RSP11048)
  10. 0.246 Hermaphrodite Research Sample1 (RSP11049)
  11. 0.246 RKM-2018-027 (RSP11119)
  12. 0.250 RKM-2018-031 (RSP11123)
  13. 0.251 Blueberry Cheesecake (RSP10684)
  14. 0.252 RKM-2018-018 (RSP11110)
  15. 0.254 RKM-2018-029 (RSP11121)
  16. 0.255 Durban Poison (RSP11014)
  17. 0.256 RKM-2018-020 (RSP11112)
  18. 0.256 Golden Goat 2 (RSP10991)
  19. 0.262 Liberty Haze (RSP11000)
  20. 0.265 RKM-2018-009 (RSP11100)

Most genetically distant strains (All Samples)

0 0.125 0.250 0.375 0.500
clone distance sibling distance more distant
  1. 0.482 Cherry Blossom (RSP11323)
  2. 0.470 Cherry Blossom (RSP11328)
  3. 0.468 Cherry Blossom (RSP11314)
  4. 0.466 Cherry Blossom (RSP11311)
  5. 0.462 Cherry Blossom (RSP11334)
  6. 0.460 Cherry Blossom (RSP11317)
  7. 0.458 Cherry Blossom (RSP11318)
  8. 0.450 Unknown- Cherry Wine - 001 (RSP11268)
  9. 0.440 Cherry Blossom (RSP11309)
  10. 0.440 Cherry Blossom (RSP11308)
  11. 0.439 Cherry Blossom (RSP11335)
  12. 0.438 Cherry Blossom (RSP11324)
  13. 0.437 Cherry Blossom (RSP11333)
  14. 0.436 Cherry Blossom CBG (RSP11303)
  15. 0.432 Cherry Blossom (RSP11312)
  16. 0.431 Cherry Blossom (RSP11301)
  17. 0.430 Cherry Blossom (RSP11330)
  18. 0.429 Cherry Blossom (RSP11327)
  19. 0.428 Cherry Blossom (RSP11325)
  20. 0.423 Cherry Blossom (RSP11274)

Most genetically distant strains (Base Tree)

0 0.108 0.217 0.325 0.433
clone distance sibling distance more distant
  1. 0.402 Cbot-2019-005 (RSP11133)
  2. 0.363 Carmagnola (RSP11037)
  3. 0.361 Feral (RSP10890)
  4. 0.357 USO 31 (RSP10981)
  5. 0.356 Cherry (RSP11142)
  6. 0.354 JL yellow (RSP11075)
  7. 0.353 Cbot-2019-001 (RSP11129)
  8. 0.351 Ivory (RSP10668)
  9. 0.351 RKM-2018-034 (RSP11126)
  10. 0.350 Santhica27 (RSP11047)
  11. 0.343 Kyrgyz Gold (RSP11054)
  12. 0.342 Monoica (RSP10241)
  13. 0.340 Skywalker OG (RSP10837)
  14. 0.338 Carmagnola (RSP10979)
  15. 0.337 Lovrin (RSP10658)
  16. 0.328 Cherry (RSP11143)
  17. 0.328 RKM-2018-026 (RSP11118)
  18. 0.326 Tisza (RSP11044)
  19. 0.326 Tisza (RSP10659)
  20. 0.326 The Gift (RSP10988)

Nearest genetic relative in Phylos dataset

Phylos Strain SRR4448285
Overlapping SNPs:
92
Concordance:
64

Nearest genetic relative in Lynch dataset

Lynch Strain SRR3495251
Overlapping SNPs:
4
Concordance:
4

Blockchain Registration Information

Transaction ID
b891672f938aa85b239ff6a5b1f65932f6fb17e2421fda9ab5b3bbfa120338fb
Stamping Certificate
Download PDF (864.3 KB)
SHASUM Hash
f06f9e8312288c635569c148eb2d2a3fd8842ab86dd4f7176ef8d507cfe7cada
QR code for RSP11122

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