Jamaican Lion

RSP 12920

Grower: kevin McKernan

General Information

Sample Name
JL-Male-Root
Accession Date
September 14, 2023
Reported Plant Sex
Male
Microbiome
Krona Plot

The strain rarity visualization shows how distant the strain is from the other cultivars in the Kannapedia database. The y-axis represents genetic distance, getting farther as you go up. The width of the visualization at any position along the y-axis shows how many strains there are in the database at that genetic distance. So, a common strain will have a more bottom-heavy shape, while uncommon and rare cultivars will have a visualization that is generally shifted towards the top.

Rarity: Rare
Most Distant Most Similar

Chemical Information

Cannabinoid and terpenoid information provided by the grower.

Cannabinoids

No information provided.

Terpenoids

No information provided.

Genetic Information

Plant Type
Type II

The bell curve in the heterozygosity visualization shows the distribution of heterozygosity levels for cannabis cultivars in the Kannapedia database. The green line shows where this particular strain fits within the distribution. Heterozygosity is associated with heterosis (aka hybrid vigor) but also leads to the production of more variable offspring. When plants have two genetically different parents, heterozygosity levels will be higher than if it has been inbred or backcrossed repeatedly.

Heterozygosity: 1.18%
Least Heterozygous Most Heterozygous

The ratio of reads mapped to Y-contigs to reads mapped to the whole Cannabis genome (Y-ratios) has been demonstrated to be strongly correlated with plant sex typing. This plot shows the distribution of Y-ratios for all samples in our database which were sequenced with the same method (panel or WGS) as this sample and where this sample falls in the distribution.

Y-Ratio Distribution: 0.0582
male female RSP12920

This chart represents the Illumina sequence coverage over the Bt/Bd allele. These are the three regions in the cannabis genome that impact THCA, CBDA, CBGA production. Coverage over the Active CBDAS gene is highly correlated with Type II and Type III plants as described by Etienne de Meijer. Coverage over the THCA gene is highly correlated with Type I and Type II plants but is anti-correlated with Type III plants. Type I plants require coverage over the inactive CBDA loci and no coverage over the Active CBDA gene. Lack of coverage over the Active CBDA and Active THCA allele are presumed to be Type IV plants (CBGA dominant). While deletions of entire THCAS and CBDAS genes are the most common Bt:Bd alleles observed, it is possible to have plants with these genes where functional expression of the enzyme is disrupted by deactivating point mutations (Kojoma et al. 2006).

Bt/Bd Allele Coverage

This chart represents the Illumina sequence coverage over the CBCA synthase gene.

CBCAS Coverage

Variants (THCAS, CBDAS, and CBCAS)

Gene HGVS.c HGVS.p Annotation Annotation Impact Contig Contig Pos Ref/Alt Var Freq
THCAS c.998C>G p.Pro333Arg missense variant moderate contig741 4416830

IGV: Start, Jump

G/C
NGS:
0.182
C90:
0.000

Variants (Select Genes of Interest)

PHL-2 c.1540A>G p.Thr514Ala missense variant moderate contig2621 340818

IGV: Start, Jump

A/G
NGS:
0.042
C90:
0.282
PHL-2 c.2756A>C p.Glu919Ala missense variant moderate contig2621 342799

IGV: Start, Jump

A/C
NGS:
0.064
C90:
0.675
PHL-2 c.3202A>C p.Thr1068Pro missense variant moderate contig2621 343245

IGV: Start, Jump

A/C
NGS:
0.053
C90:
0.000
PHL-2 c.3209A>G p.Gln1070Arg missense variant moderate contig2621 343252

IGV: Start, Jump

A/G
NGS:
0.103
C90:
0.885
PKSG-4a

UniProt

c.1000T>C p.Tyr334His missense variant moderate contig700 1938411

IGV: Start, Jump

T/C
NGS:
0.002
C90:
0.804
PKSG-2a

UniProt

c.774G>A p.Met258Ile missense variant moderate contig700 1944616

IGV: Start, Jump

C/T
NGS:
0.123
C90:
0.000
PKSG-4b

UniProt

c.352_355delACAG p.Thr118fs frameshift variant high contig700 2721317

IGV: Start, Jump

CCTGT/C
NGS:
0.529
C90:
0.000
PKSG-4b

UniProt

c.229G>A p.Gly77Ser missense variant moderate contig700 2724206

IGV: Start, Jump

C/T
NGS:
0.042
C90:
0.000
PKSG-4b

UniProt

c.216G>C p.Leu72Phe missense variant moderate contig700 2724219

IGV: Start, Jump

C/G
NGS:
0.044
C90:
0.000
PKSG-4b

UniProt

c.206T>C p.Leu69Ser missense variant moderate contig700 2724229

IGV: Start, Jump

A/G
NGS:
0.044
C90:
0.000
DXR-1

UniProt

c.139A>G p.Arg47Gly missense variant moderate contig380 235296

IGV: Start, Jump

T/C
NGS:
0.020
C90:
0.000
FAD2-2

UniProt

c.62C>T p.Pro21Leu missense variant moderate contig83 1803307

IGV: Start, Jump

G/A
NGS:
0.042
C90:
0.000
ELF3

UniProt

c.358G>A p.Gly120Arg missense variant moderate contig97 242064

IGV: Start, Jump

G/A
NGS:
0.099
C90:
0.493
ELF3

UniProt

c.520A>C p.Asn174His missense variant moderate contig97 242226

IGV: Start, Jump

A/C
NGS:
0.094
C90:
0.502
ELF3

UniProt

c.752G>T p.Gly251Val missense variant moderate contig97 242458

IGV: Start, Jump

G/T
NGS:
0.022
C90:
0.139
ELF3

UniProt

c.772A>G p.Ser258Gly missense variant moderate contig97 242478

IGV: Start, Jump

A/G
NGS:
0.112
C90:
0.000
ELF3

UniProt

c.1230-2_1230-1delAG splice acceptor variant & intron variant high contig97 243676

IGV: Start, Jump

TAG/T
NGS:
0.088
C90:
0.450
ELF3

UniProt

c.1466G>A p.Ser489Asn missense variant moderate contig97 244297

IGV: Start, Jump

G/A
NGS:
0.123
C90:
0.000
ELF3

UniProt

c.1803_1805delTCA p.His601del disruptive inframe deletion moderate contig97 244625

IGV: Start, Jump

ACAT/A
NGS:
0.092
C90:
0.000
ELF3

UniProt

c.1966C>G p.Pro656Ala missense variant moderate contig97 244797

IGV: Start, Jump

C/G
NGS:
0.123
C90:
0.632
ELF3

UniProt

c.2198G>T p.Arg733Leu missense variant moderate contig97 245029

IGV: Start, Jump

G/T
NGS:
0.125
C90:
0.531
ELF5

UniProt

c.853C>T p.Pro285Ser missense variant moderate contig382 880715

IGV: Start, Jump

C/T
NGS:
0.007
C90:
0.153
aPT4

UniProt

c.97T>C p.Tyr33His missense variant moderate contig121 2828753

IGV: Start, Jump

T/C
NGS:
0.439
C90:
0.000
aPT1

UniProt

c.864C>G p.Asn288Lys missense variant moderate contig121 2842407

IGV: Start, Jump

C/G
NGS:
0.075
C90:
0.062
HDS-2

UniProt

c.82_93delGTAACCGGAACT p.Val28_Thr31del conservative inframe deletion moderate contig95 1989748

IGV: Start, Jump

CGTAACCGGAACT/C
NGS:
0.805
C90:
0.000
PHL-1

UniProt

c.2551A>G p.Thr851Ala missense variant moderate contig1439 1487246

IGV: Start, Jump

T/C
NGS:
0.116
C90:
0.890
HDS-1

UniProt

c.136G>A p.Val46Ile missense variant moderate contig1891 889256

IGV: Start, Jump

C/T
NGS:
0.088
C90:
0.593
HDS-1

UniProt

c.56C>G p.Ala19Gly missense variant moderate contig1891 889336

IGV: Start, Jump

G/C
NGS:
0.125
C90:
0.943
HDS-1

UniProt

c.35G>A p.Cys12Tyr missense variant moderate contig1891 889357

IGV: Start, Jump

C/T
NGS:
0.094
C90:
0.646
FLD

UniProt

c.1292delG p.Gly431fs frameshift variant high contig1450 2046741

IGV: Start, Jump

TC/T
NGS:
0.000
C90:
0.000
AAE1-3

UniProt

c.475G>A p.Gly159Arg missense variant moderate contig976 1083550

IGV: Start, Jump

C/T
NGS:
0.048
C90:
0.000
AAE1-3

UniProt

c.199A>G p.Asn67Asp missense variant moderate contig976 1083876

IGV: Start, Jump

T/C
NGS:
0.079
C90:
0.000
AAE1-3

UniProt

c.188A>G p.Asn63Ser missense variant moderate contig976 1083887

IGV: Start, Jump

T/C
NGS:
0.077
C90:
0.000
AAE1-3

UniProt

c.181G>A p.Val61Ile missense variant moderate contig976 1083894

IGV: Start, Jump

C/T
NGS:
0.055
C90:
0.000
AAE1-3

UniProt

c.167A>G p.Glu56Gly missense variant moderate contig976 1083908

IGV: Start, Jump

T/C
NGS:
0.070
C90:
0.000
AAE1-3

UniProt

c.125A>G p.Glu42Gly missense variant moderate contig976 1083950

IGV: Start, Jump

T/C
NGS:
0.064
C90:
0.000
AAE1-3

UniProt

c.79A>G p.Thr27Ala missense variant moderate contig976 1083996

IGV: Start, Jump

T/C
NGS:
0.068
C90:
0.000
AAE1-3

UniProt

c.52G>A p.Gly18Ser missense variant moderate contig976 1084023

IGV: Start, Jump

C/T
NGS:
0.064
C90:
0.000
AAE1-3

UniProt

c.3G>A p.Met1? start lost high contig976 1084072

IGV: Start, Jump

C/T
NGS:
0.046
C90:
0.416
PIE1-1

UniProt

c.1222C>G p.Gln408Glu missense variant moderate contig1225 2281482

IGV: Start, Jump

C/G
NGS:
0.096
C90:
0.852
GGR

UniProt

c.704A>T p.His235Leu missense variant moderate contig2282 549696

IGV: Start, Jump

A/T
NGS:
0.121
C90:
0.000

Nearest genetic relatives (All Samples)

0 0.075 0.150 0.225 0.300
clone distance sibling distance more distant
  1. 0.043 Jamaican Lion (RSP12918)
  2. 0.053 Jamaican Lion (RSP12919)
  3. 0.216 Jamaican Lion (RSP12913)
  4. 0.223 JL 4th Gen 2 (RSP11194)
  5. 0.226 JL 4th Gen 1 (RSP11193)
  6. 0.231 JL 4th Gen 5 (RSP11199)
  7. 0.233 JL 3rd Gen Mother (RSP11214)
  8. 0.236 JL 4th Gen 4 (RSP11198)
  9. 0.240 JL yellow (RSP11075)
  10. 0.241 JL 3rd Gen Father (RSP11196)
  11. 0.243 Jamaican Lion (RSP12915)
  12. 0.244 JL 2 (RSP11076)
  13. 0.247 JL 4th Gen 7 (RSP11153)
  14. 0.251 Blueberry Cheesecake (RSP10672)
  15. 0.255 JL 3rd Gen Mother (RSP11197)
  16. 0.256 Jamaican Lion (RSP12917)
  17. 0.259 JL 4th Gen 3 (RSP11195)
  18. 0.259 Blueberry Cheesecake (RSP10671)
  19. 0.264 Blueberry Cheesecake (RSP10670)
  20. 0.273 CST (RSP11002)

Nearest genetic relatives (Base Tree)

0 0.092 0.183 0.275 0.367
clone distance sibling distance more distant
  1. 0.249 JL yellow (RSP11075)
  2. 0.251 Blueberry Cheesecake (RSP10672)
  3. 0.282 CST (RSP11002)
  4. 0.285 RKM-2018-027 (RSP11119)
  5. 0.288 Durban Poison (RSP11014)
  6. 0.292 Recon (RSP10755)
  7. 0.319 Gold Cracker (RSP11048)
  8. 0.319 UP Sunrise (RSP10989)
  9. 0.319 RKM-2018-023 (RSP11115)
  10. 0.331 Cbot-2019-001 (RSP11129)
  11. 0.331 Hermaphrodite ResearchSample2 (RSP11050)
  12. 0.332 Blue Dream (RSP11033)
  13. 0.334 Kimbo Slice (RSP10997)
  14. 0.334 Tygra (RSP10667)
  15. 0.335 KYRG-11 (RSP11051)
  16. 0.335 RKM-2018-031 (RSP11123)
  17. 0.336 Ivory (RSP10668)
  18. 0.338 Tisza (RSP10659)
  19. 0.339 RKM-2018-020 (RSP11112)
  20. 0.340 Liberty Haze (RSP11000)

Most genetically distant strains (All Samples)

0 0.125 0.250 0.375 0.500
clone distance sibling distance more distant
  1. 0.471 Cherry Blossom (RSP11323)
  2. 0.471 JL Cross 11 (RSP11512)
  3. 0.454 Cherry Blossom (RSP11318)
  4. 0.446 Cherry Blossom (RSP11306)
  5. 0.443 JL Cross 13 (RSP11514)
  6. 0.441 Chem 91 (RSP11185)
  7. 0.441 Cherry Blossom (RSP11329)
  8. 0.437 GG4 (RSP11978)
  9. 0.436 C Temple (RSP11643)
  10. 0.436 GG 4 (RSP11461)
  11. 0.433 Motor Breath 15 (RSP12093)
  12. 0.432 Super Sour Diesel (RSP11191)
  13. 0.432 Cherry Blossom (RSP11312)
  14. 0.431 Cherry Blossom CBG (RSP11303)
  15. 0.430 Sour Tsunami x Cataract Ku (RSP11183)
  16. 0.428 DB (RSP12936)
  17. 0.428 QQD2 (RSP11450)
  18. 0.427 Cherry Blossom (RSP11326)
  19. 0.427 Cherry Blossom (RSP11328)
  20. 0.427 New York City Deisel (RSP11225)

Most genetically distant strains (Base Tree)

0 0.117 0.233 0.350 0.467
clone distance sibling distance more distant
  1. 0.438 RKM-2018-002 (RSP11093)
  2. 0.415 RKM-2018-028 (RSP11120)
  3. 0.406 RKM-2018-026 (RSP11118)
  4. 0.406 RKM-2018-032 (RSP11124)
  5. 0.394 Skywalker OG (RSP10837)
  6. 0.393 RKM-2018-009 (RSP11100)
  7. 0.392 RKM-2018-018 (RSP11110)
  8. 0.391 Kush Hemp E1 (RSP11128)
  9. 0.390 RKM-2018-033 (RSP11125)
  10. 0.386 Blueberry Cheesecake (RSP10680)
  11. 0.380 Hermaphrodite Research Sample1 (RSP11049)
  12. 0.379 Cbot-2019-006 (RSP11134)
  13. 0.378 Cbot-2019-005 (RSP11133)
  14. 0.378 Carmagnola (RSP11037)
  15. 0.377 Cbot-2019-004 (RSP11132)
  16. 0.377 Feral (RSP10890)
  17. 0.376 Fedora 17 (RSP10661)
  18. 0.374 Futura 75 (RSP10664)
  19. 0.372 Black Beauty (RSP11035)
  20. 0.372 RKM-2018-019 (RSP11111)

Nearest genetic relative in Phylos dataset

Phylos Strain SRR4448297
Overlapping SNPs:
90
Concordance:
51

Nearest genetic relative in Lynch dataset

Lynch Strain SRR3495202
Overlapping SNPs:
1
Concordance:
1

Blockchain Registration Information

Transaction ID
4028a60fb6dea8a5e9418ee68d401c215e8c492586c1eb3dbd92fb4bdbe26553
Stamping Certificate
Download PDF (39.7 KB)
SHASUM Hash
45c311ca33cb42e94950a92e63833fd5d323c11c5e4cc3e4a56724e05d8d6bc2
QR code for RSP12920

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