Styrofoam Cup x Cuban Linx #4

RSP 12970

Grower: Team Elite Genetics

General Information

Sample Name
S X L #4 - 20231207
Accession Date
December 6, 2023
Reported Plant Sex
Female

The strain rarity visualization shows how distant the strain is from the other cultivars in the Kannapedia database. The y-axis represents genetic distance, getting farther as you go up. The width of the visualization at any position along the y-axis shows how many strains there are in the database at that genetic distance. So, a common strain will have a more bottom-heavy shape, while uncommon and rare cultivars will have a visualization that is generally shifted towards the top.

Rarity: Uncommon
Most Distant Most Similar

Chemical Information

Cannabinoid and terpenoid information provided by the grower.

Cannabinoids

No information provided.

Terpenoids

No information provided.

Genetic Information

Plant Type
Type I

The bell curve in the heterozygosity visualization shows the distribution of heterozygosity levels for cannabis cultivars in the Kannapedia database. The green line shows where this particular strain fits within the distribution. Heterozygosity is associated with heterosis (aka hybrid vigor) but also leads to the production of more variable offspring. When plants have two genetically different parents, heterozygosity levels will be higher than if it has been inbred or backcrossed repeatedly.

Heterozygosity: 1.13%
Least Heterozygous Most Heterozygous

The ratio of reads mapped to Y-contigs to reads mapped to the whole Cannabis genome (Y-ratios) has been demonstrated to be strongly correlated with plant sex typing. This plot shows the distribution of Y-ratios for all samples in our database which were sequenced with the same method (panel or WGS) as this sample and where this sample falls in the distribution.

Y-Ratio Distribution: 0.0315
male female RSP12970

This chart represents the Illumina sequence coverage over the Bt/Bd allele. These are the three regions in the cannabis genome that impact THCA, CBDA, CBGA production. Coverage over the Active CBDAS gene is highly correlated with Type II and Type III plants as described by Etienne de Meijer. Coverage over the THCA gene is highly correlated with Type I and Type II plants but is anti-correlated with Type III plants. Type I plants require coverage over the inactive CBDA loci and no coverage over the Active CBDA gene. Lack of coverage over the Active CBDA and Active THCA allele are presumed to be Type IV plants (CBGA dominant). While deletions of entire THCAS and CBDAS genes are the most common Bt:Bd alleles observed, it is possible to have plants with these genes where functional expression of the enzyme is disrupted by deactivating point mutations (Kojoma et al. 2006).

Bt/Bd Allele Coverage

This chart represents the Illumina sequence coverage over the CBCA synthase gene.

CBCAS Coverage

Variants (THCAS, CBDAS, and CBCAS)

Gene HGVS.c HGVS.p Annotation Annotation Impact Contig Contig Pos Ref/Alt Var Freq
THCAS c.749C>A p.Ala250Asp missense variant moderate contig741 4417079

IGV: Start, Jump

G/T
NGS:
0.127
C90:
0.632

Variants (Select Genes of Interest)

PHL-2 c.1057A>G p.Arg353Gly missense variant moderate contig2621 340335

IGV: Start, Jump

A/G
NGS:
0.096
C90:
0.555
PHL-2 c.1096G>A p.Ala366Thr missense variant moderate contig2621 340374

IGV: Start, Jump

G/A
NGS:
0.090
C90:
0.531
PHL-2 c.1540A>G p.Thr514Ala missense variant moderate contig2621 340818

IGV: Start, Jump

A/G
NGS:
0.042
C90:
0.282
PHL-2 c.2564T>A p.Phe855Tyr missense variant moderate contig2621 342607

IGV: Start, Jump

T/A
NGS:
0.064
C90:
0.732
PHL-2 c.2578T>A p.Leu860Ile missense variant moderate contig2621 342621

IGV: Start, Jump

T/A
NGS:
0.068
C90:
0.746
PHL-2 c.2756A>C p.Glu919Ala missense variant moderate contig2621 342799

IGV: Start, Jump

A/C
NGS:
0.064
C90:
0.675
PHL-2 c.2783G>A p.Ser928Asn missense variant moderate contig2621 342826

IGV: Start, Jump

G/A
NGS:
0.107
C90:
0.890
PHL-2 c.2903_2905dupGCA p.Ser968dup disruptive inframe insertion moderate contig2621 342939

IGV: Start, Jump

T/TGCA
NGS:
0.018
C90:
0.522
PHL-2 c.3002A>G p.Tyr1001Cys missense variant moderate contig2621 343045

IGV: Start, Jump

A/G
NGS:
0.055
C90:
0.316
PHL-2 c.3027G>T p.Lys1009Asn missense variant moderate contig2621 343070

IGV: Start, Jump

G/T
NGS:
0.055
C90:
0.311
PHL-2 c.3033T>G p.Cys1011Trp missense variant moderate contig2621 343076

IGV: Start, Jump

T/G
NGS:
0.055
C90:
0.292
PHL-2 c.3202A>C p.Thr1068Pro missense variant moderate contig2621 343245

IGV: Start, Jump

A/C
NGS:
0.053
C90:
0.000
PHL-2 c.3209A>G p.Gln1070Arg missense variant moderate contig2621 343252

IGV: Start, Jump

A/G
NGS:
0.103
C90:
0.885
PHL-2 c.3467A>G p.Gln1156Arg missense variant moderate contig2621 343510

IGV: Start, Jump

A/G
NGS:
0.064
C90:
0.713
PHL-2 c.3552delG p.Lys1185fs frameshift variant high contig2621 343593

IGV: Start, Jump

CG/C
NGS:
0.004
C90:
0.000
DXR-2

UniProt

c.1319T>C p.Ile440Thr missense variant moderate contig380 285250

IGV: Start, Jump

A/G
NGS:
0.480
C90:
0.000
DXR-2

UniProt

c.186A>T p.Arg62Ser missense variant moderate contig380 288577

IGV: Start, Jump

T/A
NGS:
0.000
C90:
0.000
FAD2-2

UniProt

c.64G>T p.Ala22Ser missense variant moderate contig83 1803305

IGV: Start, Jump

C/A
NGS:
0.048
C90:
0.000
ELF3

UniProt

c.358G>A p.Gly120Arg missense variant moderate contig97 242064

IGV: Start, Jump

G/A
NGS:
0.099
C90:
0.493
ELF3

UniProt

c.520A>C p.Asn174His missense variant moderate contig97 242226

IGV: Start, Jump

A/C
NGS:
0.094
C90:
0.502
ELF3

UniProt

c.772A>G p.Ser258Gly missense variant moderate contig97 242478

IGV: Start, Jump

A/G
NGS:
0.112
C90:
0.000
ELF3

UniProt

c.812G>C p.Gly271Ala missense variant moderate contig97 242518

IGV: Start, Jump

G/C
NGS:
0.114
C90:
0.938
ELF3

UniProt

c.1466G>A p.Ser489Asn missense variant moderate contig97 244297

IGV: Start, Jump

G/A
NGS:
0.123
C90:
0.000
ELF3

UniProt

c.1630A>G p.Thr544Ala missense variant moderate contig97 244461

IGV: Start, Jump

A/G
NGS:
0.103
C90:
0.545
ELF3

UniProt

c.1803_1805delTCA p.His601del disruptive inframe deletion moderate contig97 244625

IGV: Start, Jump

ACAT/A
NGS:
0.092
C90:
0.000
ELF3

UniProt

c.1966C>G p.Pro656Ala missense variant moderate contig97 244797

IGV: Start, Jump

C/G
NGS:
0.123
C90:
0.632
ELF3

UniProt

c.2141C>G p.Pro714Arg missense variant moderate contig97 244972

IGV: Start, Jump

C/G
NGS:
0.088
C90:
0.335
ELF3

UniProt

c.2198G>T p.Arg733Leu missense variant moderate contig97 245029

IGV: Start, Jump

G/T
NGS:
0.125
C90:
0.531
aPT4

UniProt

c.35A>C p.Gln12Pro missense variant moderate contig121 2828691

IGV: Start, Jump

A/C
NGS:
0.075
C90:
0.000
aPT1

UniProt

c.406A>G p.Ile136Val missense variant moderate contig121 2839605

IGV: Start, Jump

A/G
NGS:
0.579
C90:
0.761
HDS-2

UniProt

c.82_93delGTAACCGGAACT p.Val28_Thr31del conservative inframe deletion moderate contig95 1989748

IGV: Start, Jump

CGTAACCGGAACT/C
NGS:
0.805
C90:
0.000
HDS-2

UniProt

c.127T>G p.Ser43Ala missense variant moderate contig95 1989794

IGV: Start, Jump

T/G
NGS:
0.721
C90:
0.000
AAE1-2

UniProt

c.688G>A p.Asp230Asn missense variant moderate contig81 209650

IGV: Start, Jump

G/A
NGS:
0.018
C90:
0.000
AAE1-2

UniProt

c.1006A>G p.Lys336Glu missense variant moderate contig81 209968

IGV: Start, Jump

A/G
NGS:
0.116
C90:
0.000
AAE1-2

UniProt

c.1177G>A p.Asp393Asn missense variant moderate contig81 210139

IGV: Start, Jump

G/A
NGS:
0.013
C90:
0.000
PHL-1

UniProt

c.2623A>G p.Thr875Ala missense variant moderate contig1439 1487174

IGV: Start, Jump

T/C
NGS:
0.123
C90:
0.000
TFL1

UniProt

c.304G>A p.Asp102Asn missense variant & splice region variant moderate contig1636 520613

IGV: Start, Jump

C/T
NGS:
0.024
C90:
0.000
HDS-1

UniProt

c.1618A>G p.Ile540Val missense variant moderate contig1891 885936

IGV: Start, Jump

T/C
NGS:
0.099
C90:
0.861
HDS-1

UniProt

c.1378G>A p.Val460Ile missense variant moderate contig1891 886370

IGV: Start, Jump

C/T
NGS:
0.167
C90:
0.000
HDS-1

UniProt

c.56C>G p.Ala19Gly missense variant moderate contig1891 889336

IGV: Start, Jump

G/C
NGS:
0.125
C90:
0.943
PIE1-2

UniProt

c.1289A>G p.Asp430Gly missense variant moderate contig1460 1192109

IGV: Start, Jump

T/C
NGS:
0.079
C90:
0.000
EMF2

UniProt

c.1772A>G p.Gln591Arg missense variant moderate contig954 3059929

IGV: Start, Jump

A/G
NGS:
0.092
C90:
0.876
FLD

UniProt

c.2981T>C p.Met994Thr missense variant moderate contig1450 2044012

IGV: Start, Jump

A/G
NGS:
0.064
C90:
0.478
FLD

UniProt

c.2964C>A p.Asp988Glu missense variant moderate contig1450 2044029

IGV: Start, Jump

G/T
NGS:
0.057
C90:
0.464
FLD

UniProt

c.2929T>C p.Phe977Leu missense variant moderate contig1450 2044103

IGV: Start, Jump

A/G
NGS:
0.026
C90:
0.349
FLD

UniProt

c.2686G>A p.Ala896Thr missense variant moderate contig1450 2044848

IGV: Start, Jump

C/T
NGS:
0.035
C90:
0.225
FLD

UniProt

c.125G>A p.Ser42Asn missense variant moderate contig1450 2047909

IGV: Start, Jump

C/T
NGS:
0.037
C90:
0.388
AAE1-3

UniProt

c.722G>A p.Arg241Lys missense variant moderate contig976 1083132

IGV: Start, Jump

C/T
NGS:
0.070
C90:
0.000
AAE1-3

UniProt

c.659G>A p.Arg220Gln missense variant moderate contig976 1083195

IGV: Start, Jump

C/T
NGS:
0.077
C90:
0.000
AAE1-3

UniProt

c.634G>C p.Gly212Arg missense variant moderate contig976 1083220

IGV: Start, Jump

C/G
NGS:
0.116
C90:
0.000
AAE1-3

UniProt

c.199A>G p.Asn67Asp missense variant moderate contig976 1083876

IGV: Start, Jump

T/C
NGS:
0.079
C90:
0.000
AAE1-3

UniProt

c.188A>G p.Asn63Ser missense variant moderate contig976 1083887

IGV: Start, Jump

T/C
NGS:
0.077
C90:
0.000
PIE1-1

UniProt

c.1222C>G p.Gln408Glu missense variant moderate contig1225 2281482

IGV: Start, Jump

C/G
NGS:
0.096
C90:
0.852
GGR

UniProt

c.704A>T p.His235Leu missense variant moderate contig2282 549696

IGV: Start, Jump

A/T
NGS:
0.121
C90:
0.000

Nearest genetic relatives (All Samples)

0 0.058 0.117 0.175 0.233
clone distance sibling distance more distant
  1. 0.163 Garlic (RSP11341)
  2. 0.172 GMO (RSP12091)
  3. 0.176 Styrofoam Cup x Diesel Truck OG 16 (RSP12972)
  4. 0.176 Styrofoam Cup x Cuban Linx 6 (RSP12982)
  5. 0.177 Styrofoam Cup x Cuban Linx 1 (RSP12984)
  6. 0.182 Styrofoam Cup x Orange Juice 5 (RSP12974)
  7. 0.185 Skywalker OG (RSP10837)
  8. 0.195 Fatso (RSP11741)
  9. 0.198 SFVxTK (RSP11072)
  10. 0.208 Styrofoam Cup x Orange Juice 1 (RSP12980)
  11. 0.214 Red Eye OG (RSP11190)
  12. 0.215 RKM-2018-013 (RSP11104)
  13. 0.217 Rugburn OG (RSP11353)
  14. 0.219 NSPM1 (RSP11362)
  15. 0.220 Absolute OG (RSP11455)
  16. 0.221 RKM-2018-012 (RSP11103)
  17. 0.222 El Gordo (RSP12938)
  18. 0.223 GMO x Garlic Breath (RSP12507)
  19. 0.224 Pearadise x Orange Juice 4 (RSP12975)
  20. 0.225 Styrofoam Cup x Pearadise 6 (RSP12986)

Nearest genetic relatives (Base Tree)

0 0.092 0.183 0.275 0.367
clone distance sibling distance more distant
  1. 0.188 Skywalker OG (RSP10837)
  2. 0.241 RKM-2018-026 (RSP11118)
  3. 0.251 RKM-2018-034 (RSP11126)
  4. 0.256 The Gift (RSP10988)
  5. 0.264 RKM-2018-032 (RSP11124)
  6. 0.264 Pie Hoe (RSP11073)
  7. 0.272 RKM-2018-004 (RSP11096)
  8. 0.277 RKM-2018-002 (RSP11093)
  9. 0.285 Hermaphrodite ResearchSample2 (RSP11050)
  10. 0.295 Sour Raspberry (RSP10551)
  11. 0.300 RKM-2018-033 (RSP11125)
  12. 0.305 Blueberry Cheesecake (RSP10684)
  13. 0.308 Hermaphrodite Research Sample1 (RSP11049)
  14. 0.317 Blueberry Cheesecake (RSP10680)
  15. 0.319 Kimbo Slice (RSP10997)
  16. 0.324 RKM-2018-009 (RSP11100)
  17. 0.330 RKM-2018-020 (RSP11112)
  18. 0.343 RKM-2018-029 (RSP11121)
  19. 0.345 RKM-2018-022 (RSP11114)
  20. 0.346 CST (RSP11002)

Most genetically distant strains (All Samples)

0 0.133 0.267 0.400 0.533
clone distance sibling distance more distant
  1. 0.517 Cherry Blossom (RSP11318)
  2. 0.496 Unknown- Cherry Wine - 001 (RSP11268)
  3. 0.485 Cherry Blossom (RSP11325)
  4. 0.484 Cherry Blossom (RSP11323)
  5. 0.483 Cherry Blossom (RSP11274)
  6. 0.476 Tanao Sri 46 (RSP11486)
  7. 0.474 Northern Skunk (RSP11456)
  8. 0.469 Unknown- Cherry Wine - 002 (RSP11269)
  9. 0.468 AVIDEKEL 2 0 (RSP11174)
  10. 0.468 Cherry Blossom (RSP11300)
  11. 0.467 Cherry Blossom (RSP11315)
  12. 0.466 Cherry Blossom (RSP11301)
  13. 0.464 Cherry Blossom (RSP11306)
  14. 0.461 Brunswick High (RSP11164)
  15. 0.459 Cherry Blossom (RSP11319)
  16. 0.458 Cherry Blossom (RSP11312)
  17. 0.457 Cherry Blossom (RSP11331)
  18. 0.457 Ringo s Gift Katie s Cut (RSP11624)
  19. 0.454 CBD2 V 2 (RSP12669)
  20. 0.454 JL X NSPM1 12 (RSP11472)

Most genetically distant strains (Base Tree)

0 0.117 0.233 0.350 0.467
clone distance sibling distance more distant
  1. 0.456 Cbot-2019-005 (RSP11133)
  2. 0.433 JL yellow (RSP11075)
  3. 0.426 Cherry (RSP11143)
  4. 0.420 Black Beauty (RSP11035)
  5. 0.415 Cbot-2019-001 (RSP11129)
  6. 0.414 Cherry (RSP11142)
  7. 0.412 RKM-2018-023 (RSP11115)
  8. 0.409 RKM-2018-006 (RSP11097)
  9. 0.408 Cbot-2019-004 (RSP11132)
  10. 0.403 UP Sunrise (RSP10989)
  11. 0.401 Cbot-2019-006 (RSP11134)
  12. 0.401 Blueberry Cheesecake (RSP10672)
  13. 0.396 Tygra (RSP10667)
  14. 0.391 Fedora 17 (RSP10661)
  15. 0.390 Monoica (RSP10241)
  16. 0.389 RKM-2018-028 (RSP11120)
  17. 0.388 RKM-2018-018 (RSP11110)
  18. 0.386 Queen Jesus (RSP10105)
  19. 0.386 RKM-2018-019 (RSP11111)
  20. 0.386 Carmagnola (RSP11037)

Nearest genetic relative in Phylos dataset

Phylos Strain SRR8346928
Overlapping SNPs:
92
Concordance:
62

Nearest genetic relative in Lynch dataset

Lynch Strain SRR3495248
Overlapping SNPs:
3
Concordance:
3

Blockchain Registration Information

Transaction ID
36895a36109294157a0c8da3dfb4175ea3ddd61c172d7e47a644372cb17cf9f2
Stamping Certificate
Download PDF (39.6 KB)
SHASUM Hash
2f1ac34330bb34550c14a7cdb9ac08d4cb21182de70882b9e93f0a72100f2521
QR code for RSP12970

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