HM

RSP 12941

Grower: Zamir Punja

General Information

Sample Name
HM #17 roots from mother plant organic
Accession Date
September 28, 2023
Reported Plant Sex
Female
Microbiome
Krona Plot

The strain rarity visualization shows how distant the strain is from the other cultivars in the Kannapedia database. The y-axis represents genetic distance, getting farther as you go up. The width of the visualization at any position along the y-axis shows how many strains there are in the database at that genetic distance. So, a common strain will have a more bottom-heavy shape, while uncommon and rare cultivars will have a visualization that is generally shifted towards the top.

Rarity: Rare
Most Distant Most Similar

Chemical Information

Cannabinoid and terpenoid information provided by the grower.

Cannabinoids

No information provided.

Terpenoids

No information provided.

Genetic Information

Plant Type
Type I

The bell curve in the heterozygosity visualization shows the distribution of heterozygosity levels for cannabis cultivars in the Kannapedia database. The green line shows where this particular strain fits within the distribution. Heterozygosity is associated with heterosis (aka hybrid vigor) but also leads to the production of more variable offspring. When plants have two genetically different parents, heterozygosity levels will be higher than if it has been inbred or backcrossed repeatedly.

Heterozygosity: 1.46%
Least Heterozygous Most Heterozygous

The ratio of reads mapped to Y-contigs to reads mapped to the whole Cannabis genome (Y-ratios) has been demonstrated to be strongly correlated with plant sex typing. This plot shows the distribution of Y-ratios for all samples in our database which were sequenced with the same method (panel or WGS) as this sample and where this sample falls in the distribution.

Y-Ratio Distribution: 0.0234
male female RSP12941

This chart represents the Illumina sequence coverage over the Bt/Bd allele. These are the three regions in the cannabis genome that impact THCA, CBDA, CBGA production. Coverage over the Active CBDAS gene is highly correlated with Type II and Type III plants as described by Etienne de Meijer. Coverage over the THCA gene is highly correlated with Type I and Type II plants but is anti-correlated with Type III plants. Type I plants require coverage over the inactive CBDA loci and no coverage over the Active CBDA gene. Lack of coverage over the Active CBDA and Active THCA allele are presumed to be Type IV plants (CBGA dominant). While deletions of entire THCAS and CBDAS genes are the most common Bt:Bd alleles observed, it is possible to have plants with these genes where functional expression of the enzyme is disrupted by deactivating point mutations (Kojoma et al. 2006).

Bt/Bd Allele Coverage

This chart represents the Illumina sequence coverage over the CBCA synthase gene.

CBCAS Coverage

Variants (THCAS, CBDAS, and CBCAS)

Gene HGVS.c HGVS.p Annotation Annotation Impact Contig Contig Pos Ref/Alt Var Freq
THCAS c.749C>A p.Ala250Asp missense variant moderate contig741 4417079

IGV: Start, Jump

G/T
NGS:
0.127
C90:
0.632

Variants (Select Genes of Interest)

ELF3

UniProt

c.2198G>T p.Arg733Leu missense variant moderate contig97 245029

IGV: Start, Jump

G/T
NGS:
0.125
C90:
0.531
aPT1

UniProt

c.406A>G p.Ile136Val missense variant moderate contig121 2839605

IGV: Start, Jump

A/G
NGS:
0.579
C90:
0.761
HDS-2

UniProt

c.82_93delGTAACCGGAACT p.Val28_Thr31del conservative inframe deletion moderate contig95 1989748

IGV: Start, Jump

CGTAACCGGAACT/C
NGS:
0.805
C90:
0.000
HDS-2

UniProt

c.127T>G p.Ser43Ala missense variant moderate contig95 1989794

IGV: Start, Jump

T/G
NGS:
0.721
C90:
0.000
FLD

UniProt

c.2686G>A p.Ala896Thr missense variant moderate contig1450 2044848

IGV: Start, Jump

C/T
NGS:
0.035
C90:
0.225
PIE1-1

UniProt

c.1222C>G p.Gln408Glu missense variant moderate contig1225 2281482

IGV: Start, Jump

C/G
NGS:
0.096
C90:
0.852

Nearest genetic relatives (All Samples)

0 0.067 0.133 0.200 0.267
clone distance sibling distance more distant
  1. 0.199 Eucalyptus (RSP12803)
  2. 0.200 East Coast Sour Diesel (RSP12922)
  3. 0.209 RKM-2018-034 (RSP11126)
  4. 0.217 Peach Ringz (RSP12899)
  5. 0.227 RKM-2018-026 (RSP11118)
  6. 0.230 Cherry Lime Runtz (RSP12486)
  7. 0.230 Rootbeer Rolex 50 (RSP12645)
  8. 0.232 CDSD (RSP12929)
  9. 0.233 Gorilla Cookies (RSP11231)
  10. 0.237 Skywalker OG (RSP10837)
  11. 0.238 HM (RSP12940)
  12. 0.239 CDSD (RSP12935)
  13. 0.242 GMO x Zkittlez 43 (RSP11976)
  14. 0.242 Deadhead OG (RSP11463)
  15. 0.245 SFVxTK (RSP11072)
  16. 0.248 Star Dawg (RSP11352)
  17. 0.249 PK (RSP12932)
  18. 0.249 Purple Urkle (RSP12890)
  19. 0.251 JABBA S STASH (RSP11348)
  20. 0.251 RKM-2018-012 (RSP11103)

Nearest genetic relatives (Base Tree)

0 0.083 0.167 0.250 0.333
clone distance sibling distance more distant
  1. 0.210 RKM-2018-034 (RSP11126)
  2. 0.240 Skywalker OG (RSP10837)
  3. 0.244 RKM-2018-026 (RSP11118)
  4. 0.270 The Gift (RSP10988)
  5. 0.276 RKM-2018-004 (RSP11096)
  6. 0.276 Pie Hoe (RSP11073)
  7. 0.277 Hermaphrodite ResearchSample2 (RSP11050)
  8. 0.277 Blueberry Cheesecake (RSP10680)
  9. 0.280 RKM-2018-002 (RSP11093)
  10. 0.283 RKM-2018-032 (RSP11124)
  11. 0.284 Kimbo Slice (RSP10997)
  12. 0.307 RKM-2018-033 (RSP11125)
  13. 0.309 RKM-2018-009 (RSP11100)
  14. 0.313 Recon (RSP10755)
  15. 0.316 Blueberry Cheesecake (RSP10684)
  16. 0.317 Jiangji (RSP10653)
  17. 0.319 Hermaphrodite Research Sample1 (RSP11049)
  18. 0.321 Queen Jesus (RSP10105)
  19. 0.322 RKM-2018-020 (RSP11112)
  20. 0.323 CST (RSP11002)

Most genetically distant strains (All Samples)

0 0.125 0.250 0.375 0.500
clone distance sibling distance more distant
  1. 0.475 Cherry Blossom (RSP11306)
  2. 0.465 Cherry Blossom (RSP11325)
  3. 0.460 Tanao Sri 46 (RSP11486)
  4. 0.459 Cherry Blossom (RSP11328)
  5. 0.456 Cherry Blossom (RSP11318)
  6. 0.454 Cherry Blossom (RSP11330)
  7. 0.450 Cherry Blossom (RSP11323)
  8. 0.449 Cherry Blossom (RSP11312)
  9. 0.448 Cherry Blossom (RSP11309)
  10. 0.448 Cherry Blossom (RSP11322)
  11. 0.445 Cherry Blossom (RSP11298)
  12. 0.445 Cherry Blossom (RSP11300)
  13. 0.444 Wife (RSP11148)
  14. 0.443 Cherry Blossom (RSP11317)
  15. 0.440 Chematonic Cannatonic x Chemdawg (RSP11394)
  16. 0.440 Unknown- Cherry Wine - 003 (RSP11270)
  17. 0.436 Brunswick High (RSP11164)
  18. 0.431 Cherry Blossom (RSP11301)
  19. 0.431 Ringo s Gift Katie s Cut (RSP11624)
  20. 0.430 Cherry Blossom (RSP11315)

Most genetically distant strains (Base Tree)

0 0.108 0.217 0.325 0.433
clone distance sibling distance more distant
  1. 0.410 Cbot-2019-001 (RSP11129)
  2. 0.402 Cherry (RSP11143)
  3. 0.399 JL yellow (RSP11075)
  4. 0.398 Cherry (RSP11142)
  5. 0.397 RKM-2018-028 (RSP11120)
  6. 0.381 RKM-2018-031 (RSP11123)
  7. 0.380 RKM-2018-006 (RSP11097)
  8. 0.379 RKM-2018-022 (RSP11114)
  9. 0.378 Monoica (RSP10241)
  10. 0.377 RKM-2018-023 (RSP11115)
  11. 0.376 Blueberry Cheesecake (RSP10672)
  12. 0.375 RKM-2018-027 (RSP11119)
  13. 0.373 RKM-2018-018 (RSP11110)
  14. 0.372 Cbot-2019-005 (RSP11133)
  15. 0.372 Italian Kiss (RSP11034)
  16. 0.367 UP Sunrise (RSP10989)
  17. 0.367 Cbot-2019-006 (RSP11134)
  18. 0.364 Futura 75 (RSP10664)
  19. 0.364 Black Beauty (RSP11035)
  20. 0.363 Fedora 17 (RSP10661)

Nearest genetic relative in Phylos dataset

Phylos Strain SRR4451181
Overlapping SNPs:
92
Concordance:
65

Nearest genetic relative in Lynch dataset

Lynch Strain SRR3495160
Overlapping SNPs:
4
Concordance:
4

Blockchain Registration Information

Transaction ID
1b19dac248e5350d941a87b784fe0dbf3d0f4fef2e08cb6fc026d3938e0989ac
Stamping Certificate
Download PDF (39.3 KB)
SHASUM Hash
ac02da7cfdd96c664d68d885dd5e07afcf6472f85f1da094084bdc2e4d310245
QR code for RSP12941

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